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http://dx.doi.org/10.4490/ALGAE.2005.20.1.025

Analysis of Small-Subunit rDNA Sequences Obtained from Korean Peridinium bipes f. occultatum (Dinophyceae)  

Ki, Jang-Seu (Department of Life Science, Hanyang University)
Cho, Soo-Yeon (Department of Life Science, Hanyang University)
Han, Myung-Soo (Department of Life Science, Hanyang University)
Publication Information
ALGAE / v.20, no.1, 2005 , pp. 25-30 More about this Journal
Abstract
To clarify some confusions concerning identification of the Korean Peridinium species, genotypic analysis was performed with their SSU rDNA sequences. PCR was used to amplify the partial SSU rDNA of Peridinium isolates collected from three different Korean waters (Juam, Sang-sa and Togyo Reservoirs). The PCR products were allowed directly to sequence, which revealed each 942 bp of rDNA sequence. Analyses of the rDNA sequences showed that all the Korean isolates had the same genotype (100% sequence homology), and they were nearly identical to a Japanese strain of P. bipes f. occultatum (NIES 364; 99.8% sequence similarity). The sequence-based comparisons could clearly resolve P. bipes f. occultatum isolated from three different Korean waters.
Keywords
Peridinium bipes; phylogenetic analysis; small-subunit rDNA;
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