• 한국원자력학회:학술대회논문집
• /
• 한국원자력학회 2002년도 춘계공동학술발표회요약집
• /
• pp.41.1-41
• /
• 2002

• 한국원자력학회:학술대회논문집
• /
• 한국원자력학회 1999년도 추계학술발표회요약집
• /
• pp.54.1-54
• /
• 1999

• 한국식품과학회지
• /
• 제27권6호
• /
• pp.909-914
• /
• 1995

알파-락트알부민(${\alpha}-La$)의 열처리에 의한 겔 특성을 조사하기 위하여 ${\alpha}-La$ 농도, 염의 종류와 농도, 티올시약(NEM, DTT)의 농도를 달리해 $90^{\circ}C$에서 40분간 가열하여 만든 ${\alpha}-La$의 겔형성 시간과 용해성을 측정하였다. 겔형성 시간은 ${\alpha}-La$, NaCl, $CaCl_2$, DTT의 농도가 증가할수록 감소하였으나 NEM의 경우는 그와 반대로 나타났고 ${\alpha}-La$ 용액의 겔형성은 NEM $20{\sim}50\;mM$ 경우를 제외하고는 전부 40분 내에 이루어졌다. 용해성은 ${\alpha}-La$, NaCl, $CaCl_2$, DTT의 농도가 증가함에 따라 감소하였으나 NEM의 경우는 NEM의 농도증가에 따라 용해성은 증가하는 양상을 나타내었다. 표준완충용액에 용해된 겔의 용해성은 각각 $10.4{\sim}51.3%$, $9.2{\sim}35.4%$, $11.1{\sim}35.0%$, $8.0{\sim}9.5%$, 그리고 $96.8{\sim}56.2%$였고, 8M urea와 0.5% SDS를 함유한 표준완충용액에서는 더 높은 값인 $41.8{\sim}81.3%$, $41.9{\sim}64.1%$, $43.5{\sim}69.8%$, $29.6{\sim}38.5%$, 그리고 $77.4{\sim}98.9%$로 각각 나타났다. DTT를 함유한 것은 모든 조건에서 거의 100%에 달하는 용해성을 나타내었다. 이상으로 ${\alpha}-La$의 겔형성 속도와 용해성은 여러인자 즉, 단백질 농도, 염의 종류와 농도, 티올시약의 농도 등에 의해 영향을 받고, 겔형성 속도가 증가될수록 겔의 용해성은 감소함을 알수 있었다.

• 한국식품영양학회지
• /
• 제10권2호
• /
• pp.151-159
• /
• 1997

근원섬유단백질의 이온강도에 따른 Ca-ATPase 활성, Mg-ATPase 활성 및 EDTA-ATPase 활성은 오징어와 대합에서 그 차이점이 뚜렷하였으며, activity-pH curve에서 오징어 actomyosin의 Ca-ATPase 활성은 biphasic response가 소실되었고 대합의 actomyosin은 미약한 bipasic response가 나타났다. 또한 저농도의 dioxane에 의하여 오징어의 근원섬유단백질은 급격한 활성의 감소를 보였으나 대합의 근원섬유단백질은 활성이 증가되었다. 그리고 에탄올과 메탄올은 오징어와 대합의 myosin 및 MM에 대하여 저농도에서 활성을 증가시켰다. 한편 NEM으로 근원섬유단백질을 modification시키며 10-6M 이하의 NEM 농도에서는 활성이 증가되었으나 10-5M 이상의 농도가 되면 활성의 급격한 감소가 나타났다.

• 한국잠사곤충학회지
• /
• 제35권2호
• /
• pp.100-104
• /
• 1993

누에 7개 품종을 이면교배하여 F1세대의 숙잠체종과 F2 세대의 산란수에 대한 유전분석의 결과는 다음과 같다. 분산분석의 결과에서 유전적 효과인 상가적 효과, 우성 효과, 모체 효과 및 정역간의 효과는 숙잠체종과 산란수에서 모두 유의하였다. 숙잠체중과 산란수는 우성 효과가 상가적 효과보다 컸고, 광의의 유전력은 숙잠체중과 산란수에서 각각 0.986, 0.936으로 높았다. 협의의 유전력은 숙잠체중이 0.773으로 높았으나 산란수는 0.228로 낮았다. vr-Wr graph에서 숙잠체중은 불완전 우성이고, 산란수는 초우성의 유전현상을 보였다. 일반 조합능력의 효과에서 숙잠체중은 잠 107과 잠 124의 품종이, 산란수는 S1이 각각 그 효과가 정으로 컸다. 특정조합의 효과에서 숙잠체중은 S1XC51조합, S1X잠 124 조함, 잠 107X잠 108 조합이 각각 정으로 높은 효과를 보였다. 산란수는 잠107XN63 조합, S1XC51 조합, N74X잠 108 조합, 잠 107X잠 108 조합이 각각 정으로 높은 효과를 보였다.

• BMB Reports
• /
• 제31권3호
• /
• pp.258-263
• /
• 1998

Acetolactate synthase (ALS) is the common enzyme in the biosynthetic pathways leading to leucine, valine, and isoleucine. Tobacco ALS was expressed in E. coli and purified to homogeneity. The recombinant tobacco ALS was inactivated by thiol-specific reagents, N-ethylmaleimide (NEM) and 5,5'-dithio-bis-(2-nitrobenzoic acid) (DTNB). Inactivation of the ALS by NEM followed pseudo-first order kinetics and was first order with respect to the modifier. The substrate pyruvate protected the enzyme against the inactivation by NEM and DTNB. Extrapolation to complete inactivation of the enzyme by DTNB showed modification of approximately 2 out of 4 total cysteinyl residues (or 2 cysteinyl and 1 cysteinyl residues), with approximately 1 residue protected by pyruvate. The tobacco ALS was also inactivated by the tryptophanspecific reagent, N-bromosuccinimide (NBS), and was similarly protected by pyruvate. The kinetics of the inactivation was first-order with respect to NBS. The present data suggest that cysteinyl and tryptophanyl residues play a key role in the catalytic function of the enzyme.

• Journal of Korean Vacuum Science & Technology
• /
• 제6권1호
• /
• pp.43-45
• /
• 2002

We have theoretically investigated steady-state carrier transport and current self-oscillation in negative-effective-mass (NEM) p$\^$+/pp$\^$+/diodes. The current self-oscillation here is a result of the formation and traveling of electric field domains in the p base having a NEM. The dependence of self-oscillating frequency on the applied dc voltage is obtained by detailed numerical simulations. In the calculations, we have considered the scatterings by carrier-impurity, carrier-acoustic phonon, carrier-polar-phonon, and carrier-nonpolar-phonon-hole interactions . This kind of NEM oscillator allows us to reach a current oscillation with terahertz frequency, thus it may be used as a broadband source of terahertz radiation.

• 한국반도체및디스플레이장비학회:학술대회논문집
• /
• 한국반도체및디스플레이장비학회 2005년도 추계 학술대회
• /
• pp.27-30
• /
• 2005

A nanoelectromechanical (NEM) switching device based on carbon nanotube (CNT) was investigated using atomistic simulations. The model schematics for a CNT based three-terminal NEM switching device fabrication were presented. for the CNT-based three-terminal NEM switch, the interactions between the CNT-lever and the drain electrode or the substrate were very important. When the electrostatic force applied to the CNT-lever was the critical point, the CNT-lever was rapidly bent because of the attractive foroe between the CNT-lever and the drain. The energy curves for the pull-in and the pull-out processes showed the hysteresis loop that was induced by the adhesion of the CNT on the copper, which was the interatomic interaction between the CNT and the copper.

• Steel and Composite Structures
• /
• 제31권1호
• /
• pp.43-51
• /
• 2019

This paper is concerned with the numerical calculation of mixed-mode stress intensity factors (SIFs) of 2-D isotropic functionally graded materials (FGMs) by the natural element method (more exactly, Petrov-Galerkin NEM). The spatial variation of elastic modulus in non-homogeneous FGMs is reflected into the modified interaction integral ${\tilde{M}}^{(1,2)}$. The local NEM grid near the crack tip is refined, and the directly approximated strain and stress fields by PG-NEM are enhanced and smoothened by the patch recovery technique. Two numerical examples with the exponentially varying elastic modulus are taken to illustrate the proposed method. The mixed-mode SIFs are parametrically computed with respect to the exponent index in the elastic modulus and external loading and the crack angle and compared with the other reported results. It has been justified from the numerical results that the present method successfully and accurately calculates the mixed-mode stress intensity factors of 2-D non-homogeneous functionally graded materials.

• Structural Engineering and Mechanics
• /
• 제84권6호
• /
• pp.723-731
• /
• 2022

Functionally graded materials (FGMs) have been spotlighted as an advanced composite material, accordingly the intensive studies have focused on FGMs to examine their mechanical behaviors. Among them is thermal buckling which has been a challenging subject, because its behavior is connected directly to the safety of structural system. In this context, this paper presents the numerical analysis of thermal buckling of metal-ceramic functionally graded (FG) plates. For an accurate and effective buckling analysis, a new numerical method is developed by making use of (1,1,0) hierarchical model and 2-D natural element method (NEM). Based on 3-D elasticity theory, the displacement field is expressed by a product of 1-D assumed thickness monomials and 2-D in-plane functions which are approximated by NEM. The numerical method is compared with the reference solutions through the benchmark test, from which its numerical accuracy has been verified. Using the developed numerical method, the critical buckling temperatures of metal-ceramic FG plates are parametrically investigated with respect to the major design parameters.