• Proceedings of the Korean Nuclear Society Conference
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• 2002.05a
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• pp.41.1-41
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• 2002

• Proceedings of the Korean Nuclear Society Conference
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• 1999.10a
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• pp.54.1-54
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• 1999

• Korean Journal of Food Science and Technology
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• v.27 no.6
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• pp.909-914
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• 1995

In order to investigate gelation properties of Gelation Properties of ${\alpha}-lactalbumin$(${\alpha}-La$), gelling times and protein solubilities of ${\alpha}-La$ gels prepared in 0.1 M Tris-HCI buffer(pH 8.0) followed by heating at $90^{\circ}C$ for 40 minutes under different ${\alpha}-La$ concentration, NaCl, $CaCl_2$, N-ethylmaleimide(NEM), and dithiothreitol(DTT) concentration were measured. Gelling times decreased with increasing concentration of ${\alpha}-La$, NaCl, $CaCl_2$, and DTT, but increased with increasing concentration of NEM. ${\alpha}-La$ solutions made from all treatments were gelled within 40 minutes with the exception of NEM at $20{\sim}50\;mM$. Solubilities decreased with increasing concentration of ${\alpha}-La$, NaCl, $CaCl_2$, and DTT, but the solubility of NEM-modified gels increased with increasing concentration of NEM. As the results, solubilities In standard buffer were $10.4{\sim}51.3%$, $9.2{\sim}35.4%$, $11.1{\sim}35.0%$, $8.0{\sim}9.5%$, and $96.8{\sim}56.2%$, respectively. Solubilities in standard buffer containing 8 M urea and 0.5% SDS were higher than those in standard buffer, and were $41.8{\sim}81.3%$, $41.9{\sim}64.1%$, $43.5{\sim}69.8%$, $29.6{\sim}38.5%$, and $77.4{\sim}98.9%$, respectively. Solubilities in the presence of DTT were almost close to 100% in all conditions. These results indicates that the gelation rate and solubility are influenced by many factors, i.e. protein concentration, kind and concentration of salts, concentration of thiol reagents. The solubility of gel decreased with increasing the gelation rate.

• The Korean Journal of Food And Nutrition
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• v.10 no.2
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• pp.151-159
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• 1997

In order to compare and examine the general characteristics of myofibrillar proteins which is an important protein source as a food resource and relates directly with muscle contraction, we have extracted the myofibrillar proteins from squid and clam. The ionic strength of myofibrillar proteins connected with Ca-ATPase activity, Mg-ATPase activity and EDTA-ATPase activity showed distinct differences between squid and clam. In the activity-pH curve, actomyosin of the clam had a weak biphasic response. In the low concentration of dioxane, myofibrillar proteins of the squid showed a sudden decrease in activity but myofibrillar proteins of the clam showed in increase in activity. Ethanol and metanol in low concentration caused myosin and HMM from the squid and clam to increase their activities. If we cause modification by NEM, under 10-6M concentration, the activity was increased but above 10-5M concentration, there was a sudden decrease in activity.

• Journal of Sericultural and Entomological Science
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• v.35 no.2
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• pp.100-104
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• 1993

The genetics analysis for weight of matured silkworm(WMS) and number of eggs produced per moth(NEM) was studied by the seven parents diallel. Mean squares of additive effect, dominant effect, maternal effect and reciprocal effect were significant for two characters observed. The component of genetic variance analysis for WMS and NEM showed that dominant effect was higher than additive effect. Narrow sense heritability(h2ns) estimates were 0.773 and 0.228, in the WMS and NEM. The estimate of broad sense heritability(h2bs) value was higher than that of h2ns because of the low importance of dominance effect. Incomplete dominance was shown by Vr-Wr graphic analysis in the weight of matured silkworm and overdominance in the number of eggs produced per moth. In general combining ability effect, Jam 107 and Jam 124 was showed positively high for WMS and Jam 107 and S1 was expressed positively high for NEM. In specific combining ability effect, hybrids in S1XC51, S1XJam124 and Jam 107XJam 108 were exhibited positively high for WMS and Jam 107XN63, S1XC51, N74XJam 108 and Jam 107XJam 108 were found positively high for NEM.

• BMB Reports
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• v.31 no.3
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• pp.258-263
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• 1998

Acetolactate synthase (ALS) is the common enzyme in the biosynthetic pathways leading to leucine, valine, and isoleucine. Tobacco ALS was expressed in E. coli and purified to homogeneity. The recombinant tobacco ALS was inactivated by thiol-specific reagents, N-ethylmaleimide (NEM) and 5,5'-dithio-bis-(2-nitrobenzoic acid) (DTNB). Inactivation of the ALS by NEM followed pseudo-first order kinetics and was first order with respect to the modifier. The substrate pyruvate protected the enzyme against the inactivation by NEM and DTNB. Extrapolation to complete inactivation of the enzyme by DTNB showed modification of approximately 2 out of 4 total cysteinyl residues (or 2 cysteinyl and 1 cysteinyl residues), with approximately 1 residue protected by pyruvate. The tobacco ALS was also inactivated by the tryptophanspecific reagent, N-bromosuccinimide (NBS), and was similarly protected by pyruvate. The kinetics of the inactivation was first-order with respect to NBS. The present data suggest that cysteinyl and tryptophanyl residues play a key role in the catalytic function of the enzyme.

• Journal of Korean Vacuum Science & Technology
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• v.6 no.1
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• pp.43-45
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• 2002

We have theoretically investigated steady-state carrier transport and current self-oscillation in negative-effective-mass (NEM) p$\^$+/pp$\^$+/diodes. The current self-oscillation here is a result of the formation and traveling of electric field domains in the p base having a NEM. The dependence of self-oscillating frequency on the applied dc voltage is obtained by detailed numerical simulations. In the calculations, we have considered the scatterings by carrier-impurity, carrier-acoustic phonon, carrier-polar-phonon, and carrier-nonpolar-phonon-hole interactions . This kind of NEM oscillator allows us to reach a current oscillation with terahertz frequency, thus it may be used as a broadband source of terahertz radiation.

• Proceedings of the Korean Society Of Semiconductor Equipment Technology
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• 2005.09a
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• pp.27-30
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• 2005

A nanoelectromechanical (NEM) switching device based on carbon nanotube (CNT) was investigated using atomistic simulations. The model schematics for a CNT based three-terminal NEM switching device fabrication were presented. for the CNT-based three-terminal NEM switch, the interactions between the CNT-lever and the drain electrode or the substrate were very important. When the electrostatic force applied to the CNT-lever was the critical point, the CNT-lever was rapidly bent because of the attractive foroe between the CNT-lever and the drain. The energy curves for the pull-in and the pull-out processes showed the hysteresis loop that was induced by the adhesion of the CNT on the copper, which was the interatomic interaction between the CNT and the copper.

• Steel and Composite Structures
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• v.31 no.1
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• pp.43-51
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• 2019

This paper is concerned with the numerical calculation of mixed-mode stress intensity factors (SIFs) of 2-D isotropic functionally graded materials (FGMs) by the natural element method (more exactly, Petrov-Galerkin NEM). The spatial variation of elastic modulus in non-homogeneous FGMs is reflected into the modified interaction integral ${\tilde{M}}^{(1,2)}$. The local NEM grid near the crack tip is refined, and the directly approximated strain and stress fields by PG-NEM are enhanced and smoothened by the patch recovery technique. Two numerical examples with the exponentially varying elastic modulus are taken to illustrate the proposed method. The mixed-mode SIFs are parametrically computed with respect to the exponent index in the elastic modulus and external loading and the crack angle and compared with the other reported results. It has been justified from the numerical results that the present method successfully and accurately calculates the mixed-mode stress intensity factors of 2-D non-homogeneous functionally graded materials.

• Structural Engineering and Mechanics
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• v.84 no.6
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• pp.723-731
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• 2022

Functionally graded materials (FGMs) have been spotlighted as an advanced composite material, accordingly the intensive studies have focused on FGMs to examine their mechanical behaviors. Among them is thermal buckling which has been a challenging subject, because its behavior is connected directly to the safety of structural system. In this context, this paper presents the numerical analysis of thermal buckling of metal-ceramic functionally graded (FG) plates. For an accurate and effective buckling analysis, a new numerical method is developed by making use of (1,1,0) hierarchical model and 2-D natural element method (NEM). Based on 3-D elasticity theory, the displacement field is expressed by a product of 1-D assumed thickness monomials and 2-D in-plane functions which are approximated by NEM. The numerical method is compared with the reference solutions through the benchmark test, from which its numerical accuracy has been verified. Using the developed numerical method, the critical buckling temperatures of metal-ceramic FG plates are parametrically investigated with respect to the major design parameters.