• Title/Summary/Keyword: NEAT1

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Study on New Extraction Method of Microcystins from Cyanobacteria (남조류로부터 마이크로시스틴을 추출하는 새로운 추출법 연구)

  • Pyo, Dong Jin;Shin, Hyun Du
    • Journal of the Korean Chemical Society
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    • v.45 no.2
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    • pp.149-155
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    • 2001
  • A new analytical method of cyanobacterial toxins, i, e, microcysins was deveeloped using supercritical fluid extraction(SFE). The microcystins mcluded in the study are sparsely soluble in neat supercritical fluid CO$_2$ However, the microcystins were successfully extracted with a temary mixture(90% CO$_2$,9.0% methanol 1.0% water) at 40$^{\circ}$C and 250 atm. The SFE method developed in this study has several advantages over solid-phase extraction(SPE) sample preparation for the analysis of microcystins. Sample handling steps are minimized thus reducing possible losses of analytes and saving analysis time. No clean-up steps are employed in this SFE method. Althouhgh many methods have been described for microcystim RR and LR, the method using solid-phase extraction with ODS cartridges is the most commonly used. However, the adsorbing power of ODS caridges for microcystins is weak, so we have attempted to use a more polar CN cartridge, to increase the adsorbing power for microcystins. Lyophilized cells(100mg) were wxtracted with 5% (v/v) acetic acid. The extract was centrifuged and then the supernatant was applied to a CN cartridge. The cartridge which contained microcystins was rinsed with 5 ml of water and 5 ml of 0.5 M acetic acid. followed by 5 ml of 5% acetonitrile in water , and were determined by HPLC. Better recoveries and chromatogram were observed than with ODS cartridge.

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A Study on the Image of Dental Hygienists in Dental Patients and Caregivers (치과환자 및 보호자가 인지한 치과위생사 이미지)

  • Kang, Boo-Wol
    • Journal of Korean society of Dental Hygiene
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    • v.7 no.2
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    • pp.197-211
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    • 2007
  • The purpose of this study was to examine the image of dental hygienists. The subjects in the study were 396 patients and their caregivers who visited 14 dental hospitals and dentists' offices in and around Seoul. After a survey was conducted, the following findings were given: 1. The people investigated gave a mean of 4.0 to the image of dental hygienists. Dental hygienists rated highest in terms of looking neat and tidy(4.33), and were given the lowest marks in arbitrary job performance(3.46). 2. There appeared four different types of dental hygienist images, which made a 59.824 prediction of the image of dental hygienists in general. A typical image(4.11) was most dominant, followed by professional one(4.07), personal one(4.01) and social one(3.73). 3. The relations between the general characteristics of the respondents and their image of dental hygienists could be described as below: 1) By age, those who were in their 60s(4.12) had the best image of dental hygienists, and the teenagers(3.90) had the worst image of them. 2) By gender, the men(4.05) looked at dental hygienists more positively than the women(3.96). They had a significantly different opinion on the social image of dental hygienists according to gender(pE0.05). 3) By marital status, the married people(4.00) had a better image of dental hygienists than the unmarried ones(3.95). 4) As for the influence of the type of dental institutions, they had a more favorable image of dental hygienists in dentists' offices(4.13) than in dental hospitals(3.88). There was a broad significant difference in the way they looked at the professional and personal images of dental hygienists according to the type of dental institutions(pE0.001). 5) As to the impact of educational level, those who received community-college or higher education(4.01) had the best image of dental hygienists, and those who received middle-school or lower education(3.91) had the worst image of them. 6) By occupation, the government workers and students(4.07) had the best image of dental hygienists, and the company employees(3.90) had the worst image of them. They took a significantly different view of the social image of dental hygienists according to occupation(pE0.05). 7) Concerning the impact of the frequency of receiving dental treatment, the respondents who had received it seven times or more(4.16) had the best image of dental hygienists, and those who hadn't(3.79) the worst image of them. There was a wide significant gap according to that frequency. The way they looked at the typical, social and professional images of dental hygienists was quite different significantly according to that frequency as well(pE0.001). 8) As for the influence of whether they were accompanied by caregivers or not, the people who weren't accompanied by caregivers(3.99) had a better image of dental hygienists than those who were(3.97).

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A research on the emotion GUI design of touch mobile for Grooming user by using a multidimensional standard analysis (다차원 척도 분석법을 통한 Grooming 사용자의 터치폰 감성 GUI 디자인에 대한 연구)

  • Kim, Ji-Hye;Whang, Min-Cheol;Kim, Yong-Woo;Lim, Joa-Sang
    • Science of Emotion and Sensibility
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    • v.12 no.4
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    • pp.501-510
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    • 2009
  • This study is to establish GUI (graphic user interface) in mobile touch phone for grooming user by using two dimensional emotion model determined by multi-dimensional scale method. The processes conducted in the research were as the followings: First of all, visceral, behavioral, and reflective factors of emotion (Norman, 2002) was defined from investigating the life styles of the Grooming users. Secondly, factor analysis was performed to extract the representative emotional words. In the third step, they were mapped into the two-dimensional emotion model through multi-dimensional scaling. Finally, the mapped emotional words were tried to be related to GUI factors of touch phones and normalizing their relation degree between 0 and 1. This study determined GUI factors significantly related to representative emotions described as special, self-centered, sophisticated, free, passionate, neat for application to mobile touch phone. This study determined the major emotion factors that should be considered the most important while designing the GUI factors.

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Characterization of Poly(methyl methacrylate)-tin (IV) Chloride Blend by TG-DTG-DTA, IR and Pyrolysis-GC-MS Techniques

  • Arshad, Muhammad;Masud, Khalid;Arif, Muhammad;Rehman, Saeed-Ur;Saeed, Aamer;Zaidi, Jamshed Hussain
    • Bulletin of the Korean Chemical Society
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    • v.32 no.9
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    • pp.3295-3305
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    • 2011
  • Thermal behavior of poly (methyl methacrylate) was analyzed in the presence of tin (IV) chloride. Five different proportions - polymer to additive - were selected for casting films from common solvent. TG, DTG and DTA were employed to monitor thermal degradation of the systems. IR and py-GC-MS helped identify the decomposition products. The blends start degrading at a temperature lower than that of the neat polymer and higher than that of the pure additive. Complex formation between tin of additive and carbonyl oxygen (pendent groups of MMA units) was noticed in the films soon after the mixing of the components in the blends. The samples were also heated at three different temperatures to determine the composition of residues left after the expulsion of volatiles. The polymer, blends and additive exhibited a one step, two-step and three-step degradation, respectively. $T_0$ is highest for the polymer, lowest for the additive and is either $60^{\circ}C$ or $70^{\circ}C$ for the blends. The amount of residue increases down the series [moving from blend-1 (minimum additive concentration) to blend-5 (maximum additive concentration)]. For blend-1, it is 7% of the original mass whereas it is 16% for blend-5. $T_{max}$ also goes up as the concentration of additive in the blends is elevated. The complexation appears to be the cause of observed stabilization. Some new products of degradation were noted apart from those reported earlier. These included methanol, isobutyric acid, acid chloride, etc. Molecular-level mixing of the constituents and "positioning effect" of the additive may have brought about the formation of new compounds. Routes are proposed for the appearance of these substances. Horizontal burning tests were also conducted on polymer and blends and the results are discussed. Activation energies and reaction orders were calculated. Activation energy is highest for the polymer, i.e., 138.9 Kcal/mol while the range for blends is from 51 to 39 Kcal/mol. Stability zones are highlighted for the blends. The interaction between the blended parts seems to be chemical in nature.

Mechanical and Electrical Properties of Electrospun CNT/PVDF Nanofiber for Micro-Actuator (미세-작동기를 위한 전기방사 CNT/PVDF 나노섬유 기반의 탄소 복합재의 기계적 및 전기적 특성 평가)

  • Gu, Ga-Young;Wang, Zuo-Jia;Kwon, Dong-Jun;Park, Joung-Man
    • Composites Research
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    • v.26 no.1
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    • pp.14-20
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    • 2013
  • The electrospun PVDF containing CNT was made for fabricating materials of the actuator. The electrochemical and their actuating movement were evaluated for the actuator performance in the electrochemical environment. The actuator (which was fabricated by electrospinning) had some advantages, i.e., good dispersion and flexible properties. In the electrospinning process, the final product would have different forms based on different essential factors. In this work, electrospun nanofibers were aligned by using the drum-type collector, and the morphology was identified via the field emission-scanning electron microscope (FE-SEM). The uniform dispersion of CNT in PVDF nanofiber was observed by electron probe X-ray micro-analysis (EPMA) test. The results of tensile strength and electrical resistivity provided the aligned state. The electrospun CNT/PVDF nanofiber sheet on the aligned direction showed better mechanical and electrical properties than the case of the vertically-aligned direction. The efficiency and electrical capacities of electrospun CNT/PVDF nanofiber sheets were compared with the cast PVDF sheet for actuator application. Electrospun CNT/PVDF nanofiber sheet exhibited much better the case of actuator performance than cast neat PVDF actuator, due to the excellent electrical connecting areas.

An Analysis on the Empathic Changing Process of the Members in Empathy Training Program (공감훈련프로그램 참여아동의 공감표현 변화과정 분석)

  • Kim, Mi-Young
    • The Korean Journal of Elementary Counseling
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    • v.7 no.1
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    • pp.205-226
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    • 2008
  • The purpose of the study you have seen is to verify the effectiveness of existing quantitative research and to put the Empathy Training Program to practical use for participating children. From looking into this, the changes in empathic understanding that came to light in relationships between teacher and children and children and children are sure to have that effect. For this work, I established the following subject of inquiry: What kind of changing processes can be seen in the empathic understanding of participating children in the Empathy Training Program? To resolve the above line of inquiry, six female sixth grade elementary school students were chosen and they progressed through twelve sessions of the Empathy Training Program. The children were given a sentence completion exam, recognition work, neat writing exam and a school adaptation exam both before and after participation in the program, making data for analysis. To analyze, first, participants had one or two meetings of forty to fifty minutes each. Progress through the program's curriculum was recorded and through the repeating and copying method, to be sure participating children's empathic understanding was revealed, empathic language and behavior was routinely chosen. Next, according the above criteria I looked into visible changes of the participating children's empathic expressions, classifying and analyzing changes in empathic understanding and six instances of common changes in the emphatic understanding of the participants relationships were analyzed and put together. Next I will summarize the findings we have seen in this research: First, if we look into changes in common empathic understanding from the beginning, using the criteria of empathic language, each individual showed understanding at the beginning and passed and progressed through stages of care, insight and emotional expressions. Second, when we looked at the criteria of empathic behavior from the beginning to the end, one's line of vision and ability to concentrate one's attention was connected. Next, the act of nodding one's head looked like a brief nod at first but at the end, it was not just a simple nod but rather they could feel deep empathy. The condition and substance of the facial expression was seen to match and at the very end the child was expressive and stretched out arms to hold and pat the other person and the act of holding hands could also be seen. Among lots of empathic behavior the final stage was shown by half of the children. Third, from the first stage to the last stage there were many cases revealed. The more the children went the more complete their empathic language became. Their vocabulary increased and became more diverse with empathic actions. Also, when comparing actions and expressions from the beginning with the end, visible expressions became more natural and sincere at the end. The result of the research we have seen is that through receiving experience of empathic understanding, participating children showed a sense of self-confidence and they looked to make peaceful expressions while not being aggressive or defensive about problems. In addition, from understanding empathic expressions, participating children's relationships felt closer. This outcome within this group in this case will be applied and the formation of empathic understanding can be used by the children internally to solve their own problems, acquire close relationships with their teachers and others. It will also contribute to smooth classroom management.

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A Study on the Image Evaluation for the Improvement of the Landscape of Horticultural Complex in Rural Area (농촌지역 시설원예단지의 경관 개선을 위한 이미지평가)

  • Kong, Minjae;Lee, Siyoung;Kang, Donghyeon;Park, Minjung;Yun, Sungwook;Shin, Jihoon;Son, Jinkwan
    • Journal of Bio-Environment Control
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    • v.26 no.2
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    • pp.78-86
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    • 2017
  • Humans are provided with a wide range of public benefits from ecosystems and agricultural ecosystems, but the establishment of the horticulture complex could be a space that hampers the public function of the agricultural ecosystem. In this study, we sought to focus the function of landscape creation of agricultural landscape and tried to analyze the landscape of the horticulture complex. Therefore, This study aims to suggest ways to build a greenhouse complex which is being indiscreetly introduced in the agriculture landscape through environmentally-friendly manner and minimize the function of the ecosystem service. We divided the greenhouse complex into two categories of Plastic Greenhouse(v) and Glass Greenhouse(g), and compared them to the Netherland and Japan counterparts. Each image of research areas was selected by 3 pics and polled by a total of 101 people. The results of the Evaluation of Landscape Image are as shown in the figure. Netherland Glass Greenhouse scored 1.80 in terms of 'Neat' which is one of the given 15 adjectives. Study results shows that Korean Plastic Greenhouse landscapes need to endeavor Japanese vinly greenhouses and Dutch glasshouses. Consequently, an analysis on the elements of landscapes including green area, variant elements, separation distance is essential in order to improve our country's greenhouse complex landscapes. In this regard, continuous research is required to improve rural landscapes and harmonize large-scale horticultural facilities into the existing agricultural ecosystem.

A study on influential factors for dental hygiene students' image of dental hygienist (치위생과 학생들의 치과위생사 이미지에 영향을 미치는 요인 연구)

  • Lee, Kyeong-Hee;Yoon, Mi-Sook;Ha, Myung-Ok
    • Journal of Korean society of Dental Hygiene
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    • v.10 no.5
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    • pp.831-839
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    • 2010
  • Objectives : The purpose of this study was to examine dental hygiene students' image of dental hygienist and factors affecting the image of dental hygienist in an effort to provide some information useful for dental hygiene education. It's basically meant to recreate the image of dental hygienist as a professional. Methods : The subjects in this study were 310 sophomores and seniors who ere selected by convenience sampling from three different three-year-course colleges located in Gyeonggi Province, Chungcheong Province and Gwangju. After a survey was conducted, the answer sheets from 277 respondents were analyzed except for 33 incomplete ones. The collected data were analyzed with a SPSSWIN 17.0 program, and statistical data on frequency and percentage were obtained to find out the general characteristics of the subjects, and another statistical data on mean and standard deviation for each item were acquired to check their image of dental hygienist. Besides, t-test, ANOVA and Kruscal-Wallis test, one of nonparametric test, were utilized to look for connections between their general characteristics and image of dental hygienist. Results : 1. They gave a mean of 3.53 to the image of dental hygienist, which showed that the dental hygiene students took a favorable view of dental hygienists in general. 2. Their response to four items showed that they looked at the image of dental hygienist in a positive manner. The four items respectively described dental hygienists as professionals, as ones who played a crucial role in the improvement of national oral health, as ones with professional knowledge and sophisticated knowhow, and as neat and decent people. And their response to the following five items indicated that they looked at the image of dental hygienists in a negative way. One was that dental hygienists were considered to be dental nurses, and another was that the occupation of dental hygienist was a demanding and stressful job. The third was that there was no single, fixed title to call dental hygienists, and the fourth was that dental hygiene wasn't recognized as an independent area. The fifth was that the social standing of dental hygienists was sort of high. 3. In regard to the impact of the general characteristics of the dental hygiene students on their image of dental hygienist, they had a more favorable image of dental hygienist when they perceived dental hygiene in a more positive manner(p<0.001). Conclusions : In order to boost the image of dental hygienist, the working conditions of dental hygienists should be improved, and educational programs should be prepared to provided them extended continuing educational opportunities. And the kind of educational environments that could nurture dental hygienists who are convinced and have a vision should be created.

Studies on the detrimental factors affecting in vitro maturation and fertilization of bovine follicular oocytes (소 난포란(卵胞卵)의 체외성숙(體外成熟) 및 체외수정(體外受精)에 영향(影響)을 미치는 요인(要因)에 관한 연구(硏究))

  • Lee, Man-hee;Kim, Sang-keun
    • Korean Journal of Veterinary Research
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    • v.31 no.2
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    • pp.179-187
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    • 1991
  • These studies were carried out to investigate the effects of the size of follicles, semen types, capacitation methods, and additions of hormones, estrous cow serum(ECS), fetal calf serum(FCS), bovine follicular fluid(BFF) and matured cumulus cell(MCC) to the medium on in vitro maturation and fertilization of bovine follicular oocytes. The ovaries were obtained from slaughtered Korean native cows. The follicular oocytes surrounded with cumulus cells were recovered by aspirating follicular fluid from the visible follicles of diameter 3~5mm. The follicular oocytes were cultured in TCM-199 medium containing hormones, FCS, ECS, BFF and MCC for 24~48hrs. in an incubator with 5% $CO_2$ in air at $38.5^{\circ}C$ and then matured oocytes were again cultured for 18~20 hrs. with motile capacitated spermatozoas the TCF (Tyrode calcium free) solution containing $100{\mu}g/ml$ of heparin. The results obtained in these experiments were summarized as follows: 1. The oocytes classified as "A,B,C,D and Degenerative" depending morphological integrity and those 61.4%, 12.1%, 19.2%, 4.2% and 3.0% of the total oocytes recovered, respectively. The maturation and fertilization rate of the A, B, C class follicular oocytes, cultured in the TCM-199 medium supplemented with 10% FCS were 89.1%, 78.0%, 52.6% and 78.1%, 66.1, 33.3%, respectively. 2. The average number of the follicular oocytes recovered from follicles size, 1~2mm, 3~5mm and above 5mm in diameter were 67, 98 and 63, respectively. The maturation and fertilization rate of the follicular oocytes, cultured in the TCM-199 medium were 56.7%, 82.5%, 46.0% and 44.8%, 71.4%, 28.6%, respectively. 3. The fertilization and cleavage rate of the follicular oocytes, inseminated with spermatozoas of epididymal cauda, neat and frozen semen were 63.3%, 73.3%, 70.0% and 32.7%, 37.8% 38.3%, respectively. 4. The fertilization and cleavage rate of follicular oocytes, fertilized with capacitated spermatozoas by heparin, BFF and HIS methods were 70.0%, 53.8%, 34.2% and 38.3%, 23.1%. 17.1%, respectively. And the fertilization and cleavage rate were higher method of heparin. 5. The maturation and fertilization rate of follicular oocytes, cultured in the TCM-199 medium supplemented with 5%, 10%, 15%, 20% and FSH, HCG, 17, $\beta$-estradiol were 76.0~82.3% and 26.2~70.0%, and those values were higher the supplementation than non-supplementation. 6. The maturation and fertilization rate of the follicular oocytes cultured in TCM-199 medium supplemented with 5~20% ECS and FCS were 74.0~80.6%, 26.2~30.0% and 71.7~76.9%, 51.9~58.0%, and the values were higher the supplement of ECS than FCS. 7. The maturation rate (68.0~64.6%) and fertilization rate(59.6~60.4%) of follicular oocytes cultured in TCM-199 medium supplemented with 10% FCS and 20~30% BFF were higher than those of follicular oocytes cultured TCM-199 medium supplemented with 10% FCS and 10% and 50% BFF. 8. The maturation rate(76.5%) and fertilization rate (61.7%) of follicular oocytes cultured in TCM-199 medium supplemented with 10% FCS and $1{\times}10^6/ml$ MCC were higher than those of follicular oocytes cultured in TCM-199 medium supplemented with 10% FCS and $1{\times}10^{{4}{\sim}{5}}/ml$ and $1{\times}10^8/ml$ cumulus cells.

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A study on dermatologic diseases of workers exposed to cutting oil (절삭유 취급 근로자의 피부질환에 관한 연구)

  • Chun, Byung-Chul;Kim, Hee-Ok;Kim, Soon-Duck;Oh, Chil-Hwan;Yum, Yong-Tae
    • Journal of Preventive Medicine and Public Health
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    • v.29 no.4 s.55
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    • pp.785-799
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    • 1996
  • We investigated the 1,004 workers who worked in a automobile factory to study the epidemiologic characterists of dermatoses due to cutting oils. Among the workers, 667(66.4%) answered the questionaire. They are belong to 5 departments of the factory-the Engine-Work(258 workers), Gasoline engine Assembly(210), Diesel engine Assembly(96), Power train Work(86), Power train Assembly(17). We measured the oil mist concentration in air of the departments and examined the workers who had dermatologic symptoms. The results were follows; 1) Oil mist concentration ; Of all measured points(52),9 points(17.2%) exeeded $5mg/m^3$- the time-weighed PEL-and one department had a upper confidence limit(95%) higher than $5mg/m^3$. 2) Dermatologists examined 213 workers. 172 of them complained any skin symptoms at that time - itching(32.5%), papule(21.6%), scale(15.7%), vesicle(12.5%) in order. The abnormal skin site found by dermatologist were palm(29.3%), finger & nail(24.6%), forearm(16.2%), back of hand(8.4%) in order. 3) As the result of physical examination, we found that 160 workers had skin diseases. Contact dermatitis was the most common; 69 workers had contact dermatitis alone(43.1%), 11 had contact dermatitis with acne(6.9%), 10 had contact dermatitis with folliculitis(6.3%), 1 had contact dermatitis with acne & folliculitis, and 1 had contact dermatitis with abnormal pigmentation. Others were folliculitis(9 workers, 5.6%), acne(8, 5.0%), folliculitis & acne (2, 1.2%), keratosis(1, 0.6%), abnormal pigmentation (1, 0.6%), and non-specific hand eczema (47, 29.3%). 4) The prevalence of any skin diseases was 34.0 pet 100 in cutting oil users, and 13.3 per 100 in non- users. Especially, the prevalence of contact dermatitis was 23.0 per 100 in cutting oil users and 23.0 per 100 in non-users. 5) We tried patch test(standard serise, oil serise, organic solvents) on 49 patients to differentiate allergic contact dermatitis from irritant contact dermatitis and found 20 were positive. 6) In a multivariate analysis(independant=age, tenure, kinds of cutting oil), the risk of skin diseases was higher in the water-based cutting oil user and both oil user than non-user or neat oil user(odds ratio were 2.16 and 2.78, respectively). And the risk of contact dermatitis was much higher at the same groups(odds ratio were 5.16 and 6.82, respectively).

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