• Asian-Australasian Journal of Animal Sciences
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• v.27 no.9
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• pp.1254-1262
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• 2014

Lymphoid enhancer binding factor 1 (LEF-1) is a member of the T-cell specific factor (TCF) family, which plays a key role in the development of breast endothelial cells. Moreover, LEF-1 gene has been identified as a candidate gene for teat number trait. In the present study, we detected two novel mutations (NC_010450.3:g. 99514A>G, 119846C>T) by DNA sequencing and polymerase chain reaction-restriction fragment length polymorphism in exon 4 and intron 9 of LEF-1 in Guanzhong Black, Hanjiang Black, Bamei and Large White pigs. Furthermore, we analyzed the association between the genetic variations with teat number trait in these breeds. The 99514A>G mutation showed an extremely significant statistical relevance between different genotypes and teat number trait in Guanzhong (p<0.001) and Large White (p = 0.002), and significant relevance in Hanjiang (p = 0.017); the 119846C>T mutation suggested significant association in Guanzhong Black pigs (p = 0.042) and Large White pigs (p = 0.003). The individuals with "AG" or "GG" genotype displayed more teat numbers than those with "AA"; the individuals with "TC" or "CC" genotype showed more teat numbers than those with "TT". Our findings suggested that the 99514A>G and 119846C>T mutations of LEF-1 affected porcine teat number trait and could be used in breeding strategies to accelerate porcine teat number trait improvement of indigenous pigs breeds through molecular marker assisted selection.

• Proceedings of the Korean Vacuum Society Conference
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• 2016.02a
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• pp.341-341
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• 2016

The discovery of light emission in nanostructured silicon has opened up new avenues of research in nano-silicon based devices. One such pathway is the application of silicon quantum dots in advanced photovoltaic and light emitting devices. Recently, there is increasing interest on the silicon quantum dots (c-Si QDs) films embedded in amorphous hydrogenated silicon-nitride dielectric matrix (a-SiNx: H), which are familiar as c-Si/a-SiNx:H QDs thin films. However, due to the limitation of the requirement of a very high deposition temperature along with post annealing and a low growth rate, extensive research are being undertaken to elevate these issues, for the point of view of applications, using plasma assisted deposition methods by using different plasma concepts. This work addresses about rapid growth and single step development of c-Si/a-SiNx:H QDs thin films deposited by RF (13.56 MHz) and ultra-high frequency (UHF ~ 320 MHz) low-pressure plasma processing of a mixture of silane (SiH4) and ammonia (NH3) gases diluted in hydrogen (H2) at a low growth temperature ($230^{\circ}C$). In the films the c-Si QDs of varying size, with an overall crystallinity of 60-80 %, are embedded in an a-SiNx: H matrix. The important result includes the formation of the tunable QD size of ~ 5-20 nm, having a thermodynamically favorable <220> crystallographic orientation, along with distinct signatures of the growth of ${\alpha}$-Si3N4 and ${\beta}$-Si3N4 components. Also, the roles of different plasma characteristics on the film properties are investigated using various plasma diagnostics and film analysis tools.

• Journal of Life Science
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• v.24 no.9
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• pp.952-958
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• 2014

The present study investigated the anti-obesity effects of Sparassis crispa (SC) on mice fed a high-fat (HF, 45 kcal% fat) diet. Mice were fed either a normal control diet and an HF diet or an HF diet supplemented with SC (1%, 3%, and 5%) for 12 weeks. The consumption of an HF diet compared to the NC group resulted in increases in body weight, the food efficiency ratio (FER), retroperitoneal and subcutaneous fat weights, cholesterol and triglyceride levels, fecal fat, and liver lipids. However, the administration of SC significantly decreased body weight gain, food intake, FER, cholesterol and triglyceride levels, and liver lipids in a dose-dependent manner. In particular, treatment with 5% SC significantly reduced the occurrence of fatty liver deposits and steatosis, which are associated with the increased adipocyte size in mice fed an HF diet. Therefore, these results suggested that dietary supplementation with SC exerts anti-obesity effects and could be used as a functional food to control obesity.

• Journal of Practical Engineering Education
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• v.12 no.1
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• pp.117-125
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• 2020

According to the government's smart factory promotion project for small and medium-sized enterprises, more than 10,000 intelligent factories are scheduled or already built in the country and the government-led goal is to nurture 100,000 skilled workers by 2022. Smart Factory introduces numerous types of education and training courses from the supplier's point of view, such as training institutions belonging to local governments, some universities, and public organizations, in the form of an efficient resource management system and ICT technology convergence in the automated manufacturing equipment. The lack of linkage with the NCS, the standard for training, seems to have room for rethinking and direction. Results of survey is provided for the family companies of K-University in the metropolitan area and Chungnam area, and analyzes job demands by identifying whether or not they want to introduce smart factories. Defining the practitioners who will serve as a window for the introduction of smart factory technology within the company, setting up a training goal in consideration of the career path, and including the level of training required competency units, optional competency units, and training time suitable for introducing and operating smart factories. Author would like to present an NCS-based qualification design plan.

• Journal of Practical Engineering Education
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• v.8 no.1
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• pp.39-47
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• 2016

Owing to increasing concern that jobseekers are wasting too much time and effort building up so-called "spec" skills to get a decent job, many companies including state-run enterprises are now hiring rookies based on their job competency rather than test scores unrelated to the job. However, universities which are mainly responsible for training professional personnel needed for various kinds of companies and factories are reluctant to adopt NCS-based educational courses. The NCS defines how much knowledge and skill are required to work in a certain industry and then evaluates them. Although the Korean government and several junior colleges have been tentatively operating the course-based qualification system at the "industrial technician" level since 2014, the results are falling far short of diverse stakeholders' expectations. Therefore, there are urgent needs to study how to develop NCS-based curriculum at the university level, and how to strengthen the linkage between work, training, and qualification.

• Journal of Mechanical Science and Technology
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• v.20 no.4
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• pp.455-466
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• 2006

This study was an attempt to control and monitor Computerized Numerical Controller (CNC) machines anywhere and anytime for the development of a ubiquitous machine (u-machine). With a Personal Digital Assistant (PDA) phone, the machine status and machining data of CNC machines can be monitored in wired and wireless environments, including the environments of IMT2000 and Wireless LAN. Moreover, CNC machines can be controlled anywhere and anytime. The concept of the anywhere-anytime controlling and monitoring of a manufacturing system was implemented in this study for the purpose of u-manufacturing and u-machines. In this concept, the communication between the CNC controller and the PDA phone was successfully performed anywhere and anytime for the real-time monitoring and control of CNC machines. In addition, the interface between the CNC controller and the developed application module was implemented by Object linking and embedding for Process Control (OPC) and shared CNC memory. For communication, the design of a server contents module within the target CNC was based on a TCP/IP. Furthermore, the client contents module within the PDA phone was designed with the aid of embedded c++ programming for mobile communication. For the interface, the monitoring data, such as the machine status, the machine running state, the name of the Numerical Control (NC) program, the alarm and the position of the stage axes, were acquired in real time from real machines with the aid of the OPC method and by sharing the CNC memory. The control data, such as the start, hold, emergency stop, reserved start and reserved stop, were also applied to the CNC domain of the real machine. CNC machines can therefore be controlled and monitored in real time, anywhere and anytime. Moreover, prompt notification from CNC machines to mobile phones, including cellular phones and PDA phones, can be automatically realized in emergencies.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.17 no.10
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• pp.2281-2287
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• 2013

Network Coding(NC) is a new paradigm for network communication. In network coding, intermediate nodes create new packets by algebraically combining ingress packets and send it to its neighbor node by broadcast manner. Network Coding has rapidly emerged as a major research area in information theory due to its wide applicability to communication through real networks. Network coding is expected to improve throughput and channel efficiency in the wireless multi-hop network. Prior researches have been carried out to employ network coding to wireless ad-hoc network. In our study, intermediate nodes identify one-hop bidirectional flows for network coding decision. We expect that the proposed scheme shall improve decoding success rate of network coded packet. From the simulation, the proposed network coding scheme achieved better performance in terms of coding gain and packet delivery rate than traditional network coding scheme.

• Current Photovoltaic Research
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• v.8 no.1
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• pp.6-11
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• 2020

The power conversion efficiency (PCE) of a two-terminal tandem solar cell depends upon the tunnel-recombination junction (TRJ) between the top and bottom sub-cells. An optimized TRJ in a tandem cell helps improve its open-circuit voltage (V_oc), short-circuit current density (J_sc), fill factor (FF), and efficiency (PCE). One of the parameters that affect the TRJ is the buffer layer thickness. Therefore, we investigated various TRJs by varying the thickness of the buffer or intermediate layer (TRJ-buffer) in between the highly doped p-type and n-type layers of the TRJ. The TRJ-buffer layer was p-type nc-Si:H, with a doping of 0.06%, an activation energy (E_a) of 43 meV, an optical gap (E_g) of 2.04 eV, and its thickness was varied from 0 nm to 125 nm. The tandem solar cells we investigated were a combination of a heterojunction with intrinsic thin layer (HIT) bottom sub-cell and an a-Si:H (amorphous silicon) top sub-cell. The initial cell efficiency without the TRJ buffer was 7.65% while with an optimized buffer layer, its efficiency improved to 11.74%, i.e., an improvement in efficiency by a factor of 1.53.

• Molecules and Cells
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• v.37 no.7
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• pp.540-546
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• 2014

Several types of genetic and epigenetic regulation have been implicated in the development of drug resistance, one significant challenge for cancer therapy. Although changes in the expression of non-coding RNA are also responsible for drug resistance, the specific identities and roles of them remain to be elucidated. Long non-coding RNAs (lncRNAs) are a type of ncRNA (> 200 nt) that influence the regulation of gene expression in various ways. In this study, we aimed to identify differentially expressed lncRNAs in 5-fluorouracil-resistant colon cancer cells. Using two pairs of 5-FU-resistant cells derived from the human colon cancer cell lines SNU-C4 and SNU-C5, we analyzed the expression of 90 lncRNAs by qPCR-based profiling and found that 19 and 23 lncRNAs were differentially expressed in SNU-C4R and SNU-C5R cells, respectively. We confirmed that snaR and BACE1AS were down-regulated in resistant cells. To further investigate the effects of snaR on cell growth, cell viability and cell cycle were analyzed after transfection of siRNAs targeting snaR. Down-regulation of snaR decreased cell death after 5-FU treatment, which indicates that snaR loss decreases in vitro sensitivity to 5-FU. Our results provide an important insight into the involvement of lncRNAs in 5-FU resistance in colon cancer cells.

• Transactions of the Korean Society of Machine Tool Engineers
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• v.17 no.1
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• pp.7-15
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• 2008

Currently existing high-accuracy and high-speed die sets used in reciprocal press create scratches at the surface of guide posts, steel balls, and bushes due to vertical movement of balls with point-contacts between inner surface of bushes and guide posts. Consequently, accuracy of the die set and the life span of the metal mold are reduced. However, those scratches could reduce the pre-load of the steel ball. This research designed and developed a groove-type die set which improves life span of the die set by eliminating point-contacts of steel balls with guide posts. The guide post consisted of a steel-ball retainer, a steel-ball retainer stopper, a guide bush, a guide pin, a snap ring, and a spring. The steel-ball retainer has 72 holes with 8 columns of 9 holes in each column. The inner surface of the guide bush was grinded(surface roughness: $Ra\;\\;0.2{\mu}m$, accuracy: $0\;{\sim}\;-0.002mm$) after NC turning and heat treatment. Also, a line of small intermediate pocket was processed inside of the guide bush for lubrication and elimination of foreign materials. Guide grooves of steel balls were processed using a wire EDM(Electrical Discharge Machining) after heat treatment. With such a design of the guide post stated above, loads against steel balls could be dispersed greatly by the line contacts through the guide groove between the guide post and the guide bush, and the life span of the guide post could be expanded semi-permanently.