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http://dx.doi.org/10.14348/molcells.2014.0151

A Long Non-Coding RNA snaR Contributes to 5-Fluorouracil Resistance in Human Colon Cancer Cells  

Lee, Heejin (Department of Biochemistry, College of Medicine, Catholic University of Korea)
Kim, Chongtae (Department of Biochemistry, College of Medicine, Catholic University of Korea)
Ku, Ja-Lok (Cancer Research Institute and Cancer Research Center, Seoul National University)
Kim, Wook (Department of Molecular Science and Technology, Ajou University)
Kim Yoon, Sungjoo (Cancer Evolution Research Center, College of Medicine, Catholic University of Korea)
Kuh, Hyo-Jeong (Cancer Evolution Research Center, College of Medicine, Catholic University of Korea)
Lee, Jeong-Hwa (Department of Biochemistry, College of Medicine, Catholic University of Korea)
Nam, Suk Woo (Cancer Evolution Research Center, College of Medicine, Catholic University of Korea)
Lee, Eun Kyung (Department of Biochemistry, College of Medicine, Catholic University of Korea)
Publication Information
Molecules and Cells / v.37, no.7, 2014 , pp. 540-546 More about this Journal
Abstract
Several types of genetic and epigenetic regulation have been implicated in the development of drug resistance, one significant challenge for cancer therapy. Although changes in the expression of non-coding RNA are also responsible for drug resistance, the specific identities and roles of them remain to be elucidated. Long non-coding RNAs (lncRNAs) are a type of ncRNA (> 200 nt) that influence the regulation of gene expression in various ways. In this study, we aimed to identify differentially expressed lncRNAs in 5-fluorouracil-resistant colon cancer cells. Using two pairs of 5-FU-resistant cells derived from the human colon cancer cell lines SNU-C4 and SNU-C5, we analyzed the expression of 90 lncRNAs by qPCR-based profiling and found that 19 and 23 lncRNAs were differentially expressed in SNU-C4R and SNU-C5R cells, respectively. We confirmed that snaR and BACE1AS were down-regulated in resistant cells. To further investigate the effects of snaR on cell growth, cell viability and cell cycle were analyzed after transfection of siRNAs targeting snaR. Down-regulation of snaR decreased cell death after 5-FU treatment, which indicates that snaR loss decreases in vitro sensitivity to 5-FU. Our results provide an important insight into the involvement of lncRNAs in 5-FU resistance in colon cancer cells.
Keywords
5-Fluorouracil; cell viability; drug resistance; long non-coding RNAs; snaR;
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