• Journal of Embryo Transfer
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• 제16권2호
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• pp.127-131
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• 2001

The Jeju horse has been raised for centuries in Jeju island. Recently, as the number of this indigenous horse has been dropped dramatically, this breed became Natural Monument #347 to conserve and multiply this endangered breed. To provide the basic information for AI, sexual activity and semen characteristics in Jeju horse were investigated. Jeju horse semen was collected using Missouri style artificial vagina from fertile stallion.\\`she number of mount per ejaculation was 2..3$\pm$1.8, and the ejaculation time was 27.0$\pm$12.5 seconds. The total volume and gel-free volume of semen was 47.8$\pm$26.7 ml and 42.7$\pm$27.4ml, respectively, and the concentration of sperm and the total number of spermatozoa per ejaculation was 200.7$\pm$112.9$\times$10$^{6}$ ml and 7.6$\pm$3.9$\times$10$^{9}$ ml, respectively. The percentage of motile sperm and the number of live spermatozoa per ejaculation was 75.0$\pm$18.2% and 6.1$\pm$3.4$\times$10$^{9}$ ml, respectively, and the pH of gel-free semen was 7.3$\pm$0.2. The total percentage of abnormal sperm was 31.5%, and the percentage of sperm with abnormal head, midpiece and tail was 9.5$\pm$11.7%, 7.0$\pm$4.0% and 15.0$\pm$15.0%, respectively.

• Journal of the Mineralogical Society of Korea
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• 제26권4호
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• pp.273-283
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• 2013

The artificial rain (pH 4.0, pH 5.6 and pH 6.85) and weathering simulation test are applied in dolomitic marble for the prediction of deterioration of the stone monuments constructed with carbonate rock by acid rain. pH of the applied rain all increase to about neutral pH after reaction of marble. The contents of $Ca^{2+}$ and $Mg^{2+}$ have increased more than twofold in two acid rain and deionized neutral rain after reaction of marble. The weight of marble is expected to decrease $0.00037kg/m^2$ each test cycle by pH 4.0 rain. This weight reduction rate of marble is 1.4 and 3.1 times more in pH 5.6 and pH 6.85 rain respectively, and 3.7 times more in only artificial weathering test. The compressive strength of marble is expected to decrease 0.2468, 0.1791 and $0.1280kg/m^2$ per test cycle with pH 4.0, pH 5.6 and pH 6.85 rain, respectively. These results mean that more acidic rain more enfeeble the strength of marble. Dolomite and small amount of calcite are precipitated in the rains after reaction of marble.

• Proceedings of the Korean Society of Developmental Biology Conference
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• 한국발생생물학회 2009년도 특별 Symposium
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• pp.49-57
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• 2009

The cloning of canids was succeeded in 2005, several years after the birth of Dolly the sheep and also after the cloning of numerous other laboratory and farm animal species. The delay of successful somatic cell nuclear transfer (SCNT)was due to the unique reproductive characteristics of the female dogin comparison to other domestic mammals, such as ovulation of immature canine oocyte and a requirement of 25 days for the completion of meiosis within the oviduct (Holst & Phemister, 1971). When the technology for the recovery of in vivo matured oocyte was established, the application of cloning also became possible and cloned dog offspring were obtained. This report summarizes the progress of technical procedures that are required for cloning canids and the application of this technique. The first cloned dog, Snuppy, was achieved using an in vivo-matured oocyte which was enucleated and transferred with an adult skin cell of male Afghan hound. After establishment of a criterion of well-matured oocyte for the improvement of SCNT efficiency, we obtained three cloned female Afghan hound and a toy poodle cloned from 14 year-old aged Poodle using SCNT through this factor. To date, cloned dogs appeared to be normal and those that have reached puberty have been confirmed to be fertile. Through application of canine SCNT technique, first, we demonstrated that SNCT is useful for conserving the breed of endangered animal from extinction through cloning of endangered gray wolves using inter-species SCNT and keeping the pure pedigree through the cloning of Sapsaree, a Korean natural monument. Secondly, we showed possibility of human disease model cloned dog and transgenic cloned dog production through cloning of red fluorescent protein expressing dog. Finally, SCNT can be used for the propagation of valuable genotypes for making elite seed stock and pet dog. In summary, dog cloning is a reproducible technique that offers the opportunity to preserve valuable genetics and a potential step towards the production of gene targeted transgenic cloned dogs for the study of human diseases.

• Korean Journal of Environment and Ecology
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• 제24권1호
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• pp.69-77
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• 2010

The Jeongdori windbreak forests of Wando island, located between the sea and the land, are very important as ecological succession areas that protect cultivated lands and fishing villages. Among the Jeongdori windbreak forests, the rocky seashore areas are occupied by Sageretia theezans mantle community, and their inner sections by sub-tree layers such as Ligustrum japonicum and Ligustrum obtusifolium. More specifically, the inner sections of the rocky seashore areas were found to be the habitat of Ligustrum japonicum-Quercus variabilis community, Ligustrum jarponicum-Viburnum erosum community, Ligustrum japonicum-Cinnamomum japonicum community, Cinnamomum japonicum-Carpinus tschonoskii community, and Pinus densiflora-Viburnum erosum community. The average height of tree layers of the Jeongdori windbreak forests is 10.3m, and the average diameter at breath height are 30.3cm. Though the windbreak forests was artificially made, it is now virtually natural forest and deserves protection for ecological reasons. Designated as a scenic spot 3, it is always in the danger of being damaged by frequent tourist visits. So the Jeongdori windbreak forest needs to be continuous maintenance and management by the National Park Service. Considering of the precious cultural treasures of the famous pebble stones covering Gugyedeung and the naturality of the Jeongdori windbreak forests, the Jeongdori windbreak forests as a scenic spot have to designated as the national monument in future and will preserve by the Cultural Properties Administration.

• Journal of the Korean Institute of Landscape Architecture
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• 제24권2호
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• pp.86-98
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• 1996

This study is intended to revaluate old tree, five of the shot of the Natural Monument and 66 shot trees under protection in the city at present. The study covers distribution, management of usage, investigation and research into various matters relating to the situation of old trees in Pusan. Because 60% of the old trees selected for protection is on private land, citizens have difficulty gaining access to them. Also space for growth is impinged on by various urban facilities. Some trees have problems of growth because the land is covered with cement and asphalt. As regards space for old trees, many places have been destroyed due to the lack of protection fences and the failure to establish a guidance board. Only 40% of the space has been set aside for rest. In these places the establishment of rest areas, benches and handy chairs is needed. And there are 20 holy places where people observe religious ceremonies. As for space security necessary for old trees according to trunk width, the minimum trunk width and availale area needed are more than 1:1.5, but less than 30% have the necessary space. The growth of many old trees is threatended because of the small apace for growth. The prescription of the boundary space is divided between inside and outside. This indicates the domain nature and space rank of the old tree. It can be classified into five forms for research purposes. The elements for prescription of critical space outside are connected with the security of space, and the elements relating to inside space portray the conditions for sensitive growth according to the institution and the ground conditions. The open and closed nature of old tree space has some connection with the relationship between usage and the intimacy of the user. Most of the spaces for old trees can't be used or are difficult to gain access to, so it is necessary to secure enough space for them. The 18 places, despite having easy access, are near farmland, hills or forest. So the intimacy factor is low, and the security of space of the old trees is not generally sufficient. Henceforth, it will be necessary to have security of area for old trees as well as maintenance control to protect them. Also the space should be used as a communal and recreation are in the city. It is the time to construct a landscaped area which people can easily approach, a place with a secure entrance where they can play, rest and converse.

• Korean Journal of Environment and Ecology
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• 제21권3호
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• pp.290-298
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• 2007

The results of this research on the diversity, zonation of vegetation and micro-topography by TWINSPAN classification and DECORANA ordination, executed with Sinduri coastal dunes of Korea, are as follows: The vegetation and micro-topography of coastal dunes formed a noticeably clear zonation structure. The beach in the direction of the coastline saw a lot of appearance of Salsola komarovi and the primary dune was dominated by Elymus mollis. Imperata cylindrica var. koenigii and Carex pumila formed a colony at flat area of the sand hills and Calamagrostis epigeios was widely distributed at the wet slack. The secondary dune was dominated mostly by Ischaemum anthephoroides and Imperata cylindrica var. koenigii, and it showed an aspect of the distribution of Vitex rotundifolia and Rosa rugosa. while the hinterland hillside in the direction of inland was dominated by Robinia pseudo-acacia and Pinus thunbergii. However, Carex kobomugi, known as the pioneer species of the coastline-bound areas at the coastal dune, dominantly occupied the secondary dune of the rear side and continentally-inclined Miscanthus sinensi and Oenothera biennis of naturalized plant were irregularly spread over the whole of the coastal dune, so the stabilization of micro-topography seemed to be uncertain. Particularly, Miscanthus sinensis was predicted to be changed into dominant species of the primary dune, and secondary dune and slack having a commonly high species gathering inclination with the more progress of stabilization of the coastal dune. The expansion of sand hill wetlands and roads located between the primary dune and secondary dune was judged to have an effect on the zonation structure of plant distribution.

• Korean Journal of Plant Resources
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• 제18권2호
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• pp.315-324
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• 2005

The study was carried out to offer the raw data on counterplan of restoration and conservation by systematical and objective analyzing the flora and herbaceous communities around Abeliophyllum distichum habitats. In the results of flora survey, as the site that the most taxa were showed was Yulji-ri, Geosan-gun III, there was surveyed as 114 ones; 48 families, 91 genera, 99 species and 15 varieties, and as Younjeong-ri, Jincheon-gun V was showed the fewest ones, there was appeared 54 ones; 28 families, 49 genera, 47 species and 7 varieties. The dominant species of herbaceous communities were verified Humulus japonicus${\cdot}$Artemisia princeps var. orientalis(Chujeom-ri, Goesan-gun I), Oplismenus undulatifolius(Songdeok-ri, Goesan-gun II and Maechon-ri, Yeongdong-eup IV), Carex siderosticta${\cdot}$Pueraria thunbergiana${\cdot}$Artemisia princeps var. orientalis(Yulji-ri, Geosan-gun III), Streptolirion cordifolium(Younjeong-ri, Jincheon-Eun V) and Sasa borealis(Junggye-ri, Buan-gun IV). In correlation analysis, Artemisia princeps var. orientalis${\cdot}$Carex siderosticta, Sasa borealis${\cdot}$Festuca ovina, Sasa borealis${\cdot}$Smilax china and Festuca ovina${\cdot}$Smilax china were showed the highest relativity. In similarity index analysis, as the site that was showed highest value was Yulji-ri, Geosan-gun III and Maechon-ri, Yeongdong-eup IV, there was turned up about some $27.27\%$. In the results of regression analysis between environmental factors and number of species, R-square of altitude and number of species was some $70.0\%$.

• Korean Journal of Plant Resources
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• 제15권2호
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• pp.114-122
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• 2002

This study investigated the species composition and distribution of woody plants, and flora of vascular plants of Cheonjiyeon where is located in the most southern region of Korea. Twenty-nine taxa of trees were distributed in the valley, and of these, deciduous broad-leaved trees were 13. Of the total number of 2,547 trees, 1,429 were deciduous broad-leaved trees and remainder was broad-leaved evergreen. Deciduous broad-leaved trees covered 48.7% of the tree canopy while evergreen broad-leaved trees covered only 42.3%. The total number of shrub taxa was 37 with more evergreen shrub taxa than deciduous. Evergreen shrubs occupied 64.4％ of shrub canopy while deciduous shrubs covered only 35.4％. The result of the investigation of flora showed that there are 446 taxa of flora including 114 families, 320 genera, 397 species, 1 subspecies, 39 varieties, and 9 forma. Of these, two species, Rubus hongnoensis Nakai and Lycoris chejuensis K. Tae et S. Ko, are endemic plants of Jeju Island. The flora includes 17 taxa of rare plants that are distributed only in Jeju Island in Korea. Also, the flora includes 1 taxon, Elaeocarpus sylvestris (Loureiro) Poiret var. ellipticus (Thunb.) Hara, of Korean natural monument, 2 taxa, Psilotum nudum (L.) Griseb. and Crypsinus hastatus (Thunb.) Copeland, of legally protected wildlife and plants, 38 taxa of naturalized plants, and 75 taxa of introduced plants.

• Korean Journal of Environment and Ecology
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• 제27권1호
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• pp.71-84
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• 2013

This study investigated some of the specific ecological characteristics Torreya nucifera forest and its health conditions so that was drawn from four different management strategies, such as Types A, B, C, and D, for protected areas of forest genetic resources. Type A refers to passive management which routinely keeps the current status of forests by removing competitive trees or curing damaged ones. Type B, as active one which improves Torreya nucifera forest, includes increase of soil pH and removal of Sasa borealis, Phyllostachys bambusoides, and deciduous broad-leaved trees such as Quercus variabilis, Carpinus tschonoskii et al.. Type C is another passive one which reduces soil acidity within Chamae cyparisobtusa forest and maintains the existing forests. Type D is another active one which decreases soil acidity and simultaneously reduces problems associated with competitive and invasive plants to nurture the young trees. At last, it is important to note that the protected areas for forest genetic resources need to be entitled to categories III(natural monument) or IV(habitat/species management area) in accordance with protected area management guidelines of IUCN.

• Journal of the Mineralogical Society of Korea
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• 제23권1호
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• pp.63-71
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• 2010

The artificial rains having different pH and weathering simulation test were performed for Gyeongju Namsan granite and dacitic tuff to predict the role of acid rain in the deterioration of stone monuments located in Gyeongju area. The pH 4.0 rain reacted with the fresh granite showed near neutral pH at the early stage due to the hydrolysis of minerals in the rock surface. But the pH changed back to the initial pH in the later stage. On the other hand, the pH 5.6 rain showed the neutral pH for a longer time than the experiment with pH 4.0 rain, reflecting slower reaction of minerals due to the weaker acidity. When the pH 5.6 rain reacted with the weathered granite, the water showed neutral pH longer than the case of the fresh granite. The similar tendencies were observed in the experiment of dacitic tuff, except that the pH 4.0 rain reacted with dacitic tuff took a longer time to go back to the initial pH as compared with the case of granite. These results may due to the differences in mineral composition and texture of two rocks. Dacitic tuff contains more fine-grained or glassy groundmass than granite and is more reactive with weaker acid rain. It was predicted that the weight loss and strength decrease rate of dacitic tuff would be approximatetly twice relative to those of granite in the same experimental environment.