• Geomechanics and Engineering
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• 제27권5호
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• pp.497-509
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• 2021

A large deformation FEM (Finite Element Method) based numerical analysis has been performed to study the behaviour of the bell-shaped anchor embedded in undrained saturated (cohesive) soil with the help of finite element based software ABAQUS. A typical model anchor with bell-diameter of 0.125 m, embedded in undrained saturated soil with varying cohesive strength (from 5 kN/m² to 200 kN/m²) has been chosen for studying the characteristic behaviour of the bell-shaped anchor installed in cohesive soil. Breakout factors have been evaluated for each case and verified with the results of experimental model tests for three different types of soil samples. The maximum value of breakout factor was found as about 8.5 within a range of critical embedment ratio of 2.5 to 3. An explicit model has been developed to estimate the breakout factor (F_c) for uplift capacity of bell-shaped anchor within clay mass in terms of H/D ratio (embedment ratio). It was also found that, the ultimate uplift capacity of the anchor increases with the increase of the value of cohesive strength of the soil and H/D ratio. The empirical equation developed in the present investigation is usable within the range of cohesion value and H/D ratio from 5 kN/m² to 200 kN /m² and 0.5 to 3.0 respectively. The proposed model has been validated against data obtained from a series of model tests carried out in the present investigation. From the stress-profile analysis of the soil mass surrounding the anchor, occurrence of stress concentration is found to be generated at the joint of anchor shaft and bell. It was also found that the vertical and horizontal stresses surrounding the anchor diminish at about a distance of 0.3 m and 0.15 m respectively.

• Journal of Microbiology and Biotechnology
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• 제15권6호
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• pp.1258-1266
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• 2005

The present study was carried out in order to assess the protective effects of calycosin-7-O-$\beta$-D-glucopyranoside, isolated from Astragali radix (AR), on hyaluronidase (HAase) and the recombinant human interleukin-$1\beta$ (IL-$1\beta$)-induced matrix degradation in human articular cartilage and chondrocytes. We isolated the active component from the n-butanol soluble fraction of AR (ARBu) as the HAase inhibitor and structurally identified as calycosin-7-O-$\beta$-D-glucopyranoside by LC-MS, IR, ${1}^H$ NMR, and ${13}^C$ NMR analyses. The $IC_{50}$ of this component on HAase was found to be 3.7 mg/ml by in vitro agarose plate assay. The protective effect of ARBu on the matrix gene expression of immortalized chondrocyte cell line C28/I2 treated with HAase was investigated using a reverse transcription polymerase chain reaction (RT-PCR), and its effect on HAase and IL-$1\beta$-induced matrix degradation in human articular cartilage was determined by a staining method and calculating the amount of degraded glycosaminoglycan (GAG) from the cultured media. Pretreatment with calycosin-7-O-$\beta$-D-glucopyranoside effectively protected human chondrocytes and articular cartilage from matrix degradation. Therefore, calycosin-7-O-$\beta$-D-glucopyranoside from AR appears to be a potential natural ant-inflammatory or antii-osteoarthritis agent and can be effectively used to protect from proteoglycan (PG) degradation.

• 약학회지
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• 제40권4호
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• pp.422-427
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• 1996

The anti-Ex-A IgG was specifically labeled with stable isotopes, DL-His-2,4-$d_2$, L-Phe-$d_5$, L-Trp-$d_5$, L-Tyr-2,6-$d_2$ and L-[1-$^{13}C$]Trp, by growing hybridoma cell in serum-free medium. By use of NMR spectroscopy with selectively labeled Fab fragment, we applied a paratope mapping on antigen-antibody complex. Assignments of the observed carbonyl carbon resonances have been determined by using $^{13}C$-$^{15}N$ double labeling method in order to assign the Trp resonances. Photo CIDNP was also applied to investigate the antigen-binding site(s) on the surface residues of antibody. We found that Trp 36, which is located at the $V_H$ domain, is an important residue to bind to Ex-A, however, two Tyr on the surface of anti-Ex-A IgG plays no crucial role to bind to antigen. On the basis of these results, we demonstrate that stable isotope-aided NMR strategy can be extended to molecular structural analyses of the complex of an Fab fragment and a protein antigen.

• 대한전기학회:학술대회논문집
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• 대한전기학회 1996년도 하계학술대회 논문집 B
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• pp.847-849
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• 1996

As the power demands increases, one of the most important data is inside electric field of equipment in designing of insulators ami insulated wire for EHV distribution line. So far finite element analysis method is widely used to calculate this electric field. However as the shape of insulator becomes complicated, it is difficult in producing the mesh which suitable the shape. Especially, we have many difficulty that produce dense fine mesh only where the electric field is concentrated. Therefore in this paper, we perform the each conditional analysis of electric field using the Maxwell 3D Simulator to recover this defects. And we try to analyze electric field through the conventional 2 dimensional and 3 dimensional analysis in case of salt contamination on the surface of a insulator.

• 한국재료학회:학술대회논문집
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• 한국재료학회 2009년도 춘계학술발표대회
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• pp.33.2-33.2
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• 2009

Dielectric and piezoelectric properties of Lead-free $(1-x)(Bi_{0.5}K_{0.5})TiO_3-xBiFeO_3$ceramics prepared by a conventional solid state reaction method were investigated in the range of x = 0~10 mol%. Piezoelectric coefficient was increased from 31 pC/N at x = 0 mol% to 64 pC/N at x = 6 mol% then decreased with increasing x. Electromechanical coupling factor ($K_p$) was increased up to 0.18 at x = 10 mol%. On the other hand, mechanical quality factor ($Q_m$) was decreased. Grain size was not much changed with various x and a single perovskite with tetragonal symmetry was maintained at all compositions forming a solid solution between $(Bi_{0.5}K_{0.5})TiO_3$ and $BiFeO_3$. Depolarization temperature ($T_d$) was gradually decreased with increasing x from $302^{\circ}C$ at x = 0 to $245^{\circ}C$ at x = 10 mol%.

• 미생물학회지
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• 제26권2호
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• pp.73-81
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• 1988

$\alpha$-Amylase (EC.3.2.1.1) of Neurospora crassa (ATCC9279) was cloned in E. coli HB101 using shotgun method, and the enzymes isolated from both N. crassa and E. coli were compared. Chromosomal DNA isolated from the spores of N. crassa was partially digested with PstI restriction endonuclease and rejoined to pBR322 which had been digested with the same enzyme. The resulting recombinant DNA were introduced into E. coli HB101 which had competancy by treating with $CaCl_{2}$. As the result, about 8000 colonies which showed tetracycline resistance were selected and two of the colonies which had 13.5Kb recombinant plasmid exhibit starch degrading activity on starch-containing plate when treated with D-cycloserine. $\alpha$-Amylases from both N.crassa and E. coli were isolated by using ammonium sulfate precipitation, DEAE-cellulose ion exchange column chromatography and Bio-Gel P150 gel foltration column. As the result, about 81.3 fold and 5.6 fold purifications in specific activities were obtained respectively, and specific activities of the gel filtrates were 6.1u/mg and 85u/mg respectively. The properties of both enzymes were compared and they showed quite the similar patterns in optimal temperature, optimal pH and had same molecular weight about 100,000 daltons on gel filtration method. Optimal temperatures for both enzymes were $70^{\circ}C$ and optimal pH were about 6 and 10.

• 대한전자공학회논문지
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• 제21권2호
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• pp.71-82
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• 1984

2차원 n-p-n 바이폴라 트랜지스터의 수치해석을 위한 프로그램(BIPOLE)을 개발하였다. 이 프로그램은 SRH와 Auger 재결합 기구들과 불순물 농도와 전계강도에 대한 운송자 이동도의 의존성과 밴드 갭 축소 효과들을 포함하고 있다. Poisson 방정식에는 Newton법을 또 정공과 전자의 연속 방정식에는 발산이론을 이용하여 여러가지 물리적인 제한없이 기본 반도체 방정식들에 대한 유한차분 공식들을 만들었다. 선형화된 방정식들의 계수 행렬은 희소 대칭 M 행렬이었는데 그 해를 구하기 위해 ICCG법과 Gummel의 알고리즘을 적용하였다. 이 프로그램 BIPOLE를 n-p-n 트랜지스터의 여러가지 정상 상태 문제에 적용시켰다. 그 응용의 보기로서 공통 에미터 전류이득의 변화, 에미터 용량에 대한 확산용량이 미치는 영향과 입출력 특성곡선들을 계산해 보았다. 전위 분포와 전자와 정공 농도분포와 같은 계산 결과를 3차원 컴퓨터 그래픽으로 도시하였다. 이 프로그램은 장차 2차원 트랜지스터의 교류 및 왜곡 현상의 수치해석의 기초로 이용될 것이며, 이 프로그램에 관심있는 모든 분들께 공급될 것이다.

• 유체기계공업학회:학술대회논문집
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• 유체기계공업학회 2001년도 유체기계 연구개발 발표회 논문집
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• pp.482-487
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• 2001

Leaf type foil bearings have been used successfully in many aerospace applications such as air cycle machines, turbocompressors and turboexpander. These applications are characterized by light loads, constant speeds and low to moderate temperatures. But, as system on start-up or shutdown, sliding contact between the shaft and foil surfaces cause wear. So, in present study, to understand pressure-flow characteristics and deformation of foil bearing, flow/structure interaction analysis was used. and using this method, 2D and 3D calculation was peformed for shape of foil bearing to know circumferential direction flow and leakage flow characteristics of axial direction.

• Nuclear Engineering and Technology
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• 제40권4호
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• pp.299-304
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• 2008

A detection system was set up to measure the neutron generation rate of a recently developed D-D neutron generator. The system is composed of a Si detector, He-3 detector, and electronics for pulse height analysis. The neutron generation rate was measured by counting protons using the Si detector, and the data was crosschecked by counting neutrons with the He-3 detector. The efficiencies of the Si and He-3 detectors were calibrated independently by using a standard alpha particle source $^{241}Am$ and a bare isotopic neutron source $^{252}Cf$, respectively. The effect of the cross-sectional difference between the D(d,p)T and $D(d,n)^3He$ reactions was evaluated for the case of a thick target. The neutron generation rate was theoretically corrected for the anisotropic emission of protons and neutrons in the D-D reactions. The attenuations of neutron on the path to the He-3 detector by the target assembly and vacuum flange of the neutron generator were considered by the Monte Carlo method using the MCNP 4C2 code. As a result, the neutron generation rate based on the Si detector measurement was determined with a relative uncertainty of ${\pm}5%$, and the two rates measured by both detectors corroborated within 20%.

• 설비공학논문집
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• 제11권1호
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• pp.31-37
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• 1999

In order to predict thermodynamic performance of refrigeration system, it is required to know the oil concentration of the refrigerant/oil mixture. The current method to measure the oil concentration is to extract the working mixture and then to measure the oil weight. However, it is Quite necessary to estimate oil concentration without any extraction of the working fluid. In this study a new method and working equation is presented as follows. It is based on the measurement of spedific gravity and temperature : $$C=a+b{\times}t+c{\times}t^2+(d+e{\times}t+f{\times}t^2){\times}SG$$ C is oil concentration, t is temperature($^{\circ}C$), SG is specific gravity of mixture and a~f is coefficients. The oil concentration ranges over 0~12 wt% and the temperature ranges over $20{\sim}50^{\circ}C$. The specific gravity and temperature are measured using the on-line densimeter and thermometer. This working equation enables to predict the oil concentration without any extraction of the mixture. This equation can be applied for R-12/Naphthenic oil and R-134a/POE oil oiquid mixtures.