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Efficent Synthetic Method of Obtaining Oligosaccharide Units and Derivatives Utilizing Endoglycosidases

  • Murata, Takeomi;Usui, Taichi
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.7 no.5
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    • pp.263-267
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    • 2002
  • Our purpose is to develop an efficient synthetic method of obtaining oligosaccharide unit in sufficient amounts to study functions of glycans. Many exoglycosidases have been used as tools for the oligosaccharide synthesis. In contrast, a limited number of reports are available on the utilization of endoglycosidases. We describe herewith the efficient synthetic method of useful oligosaccharides and derivatives as biomaterials utilizing lysozyme, cellulase, and lacto-N-biosidase-mediated transglycosyltions.

Improvement of reconfiguration rate using pseudo faulty processing elements on the single track 2-D systolic array (의사결함처리요소를 이용한 단일트랙 이차원 시스토릭 어레이에서 재구성율의 향상)

  • 신동석;우종호
    • Journal of the Korean Institute of Telematics and Electronics A
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    • v.33A no.2
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    • pp.163-172
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    • 1996
  • In reconfiguration of systolic arrays, a potential disadvantage is that in the PRESENCE of consective faulty PE's logically connected PE's may be far apart, requiring the reduction of clock speed and thus reducing throughput of the array. Thus it is fundamental tokeep locality of interconnections as high as possible even after reconfiguration and to make reconfiguration implemented in the simple routing devices. However requirements of locality and simplicity mean that reconfiguring capability is limited. This paper deals iwth the issue of developing efficient method for reconfiguration of 2-D systolic arrays which can be achieved high reconfiguration rate, with the two conditions satisfying using concept of pseudo faulty processing element. Applying this concept to reconfiguration of systolic array, we have found similar condition. The simulation shows that recomfiguration rates are 97%, 84% when N faults ocurs on the N$\times$N array n case of N=5, 8 respectively.

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A Study on Electric Characteristics of Plasma Electon Beam Produced by Cold Cathode. (냉음극을 이용한 plasma전자 beam의 전기적 입력특성 I)

  • 전춘생;박용관
    • 전기의세계
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    • v.27 no.3
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    • pp.36-42
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    • 1978
  • It has been investigates that electric characteristics of plasma electron beam in N$_{2}$, H$_{2}$ and Ar gas jars under various gas pressures during electron beams are formed. The results are as follows: 1)Electron beam is formed in the region of positive resistance on the characteristic curve. This phenomenon is identical in N$_{2}$, H$_{2}$ and Ar gases. 2)But in Ar gas, electron beam is formed at relatively lower gas pressure than in H$_{2}$ and N$_{2}$. 3)In pure gas either N$_{2}$, H$_{2}$ and N$_{2}$ the lower the gas pressure, the higher the voltage drop for the same electron beam current. 4)The region in which electron beam is formed is limited at a given pressure. 5)Beyond the limit mentioned above, it becomes glow discharge state and the current increases radically. 6)At a given gas pressure, electron beam voltage, that is, electrical power input increases with gap length.

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Investigation of thermodynamic analysis in GaN thick films gtowth (GaN 후막 증착의 열역학적 해석에 관한 연구)

  • Park, Beom Jin;Park, Jin Ho;Sin, Mu Hwan
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.8 no.3
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    • pp.387-387
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    • 1998
  • This paper reports on a thermodynamic analysis for the GaN thick film growth by vapor phaseepitaxy method. The thermodynamic calculation was performed using a chemical stoichiometric algorism. Thesimulation variables include the growth temperature in a range 400~1500 K, the gas ratios $(GaCl_3)/(GaCl_3+NH_3)$and $(N_2)/(GaCl_3+NH_3)$. The theoretical calculation predicts that the growth temperature of GaN be in thelower range of 450~750 K than the experimental results. The difference in the growth temperature betweenthe simulation and the experiments indicates that the vapor phase epitaxy of GaN is kinetically limited,presumably, due to the high activation energy of thin film growth.

Stable Nitrogen Isotopes in a Forested Watershed in Taiwan

  • Owen, Jeffrey S.
    • Journal of Forest and Environmental Science
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    • v.29 no.2
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    • pp.116-124
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    • 2013
  • Differences in rates and patterns of nitrogen cycling have been correlated with nitrogen stable isotope measurements in forest ecosystems of tropical and temperate regions, but limited similar work has been conducted in sub-tropical forests. This study investigated patterns in stable N isotopic composition in a subtropical forest in Taiwan by sampling three soil profiles and overstory and understory foliage. Soil ${\delta}^{15}N$ in the forest floor ranged from -1.8 to -1.8‰. Mineral soils had higher ${\delta}^{15}N$ (4.1 to 6.0‰). Foliage ${\delta}^{15}N$ in overstory trees ranged from -6.6 to -2.0‰, and understory foliage ${\delta}^{15}N$ ranged from -5.0 to -1.2‰. There was a weak correlation between foliar % N and ${\delta}^{15}N$ ($r^2=0.214$). Compared to results from similar surveys in tropical and temperate forests, foliar ${\delta}^{15}N$ values were generally lower. These results help highlight the need for improved knowledge regarding the relationships between patterns in N stable isotopes and processes affecting rates of N cycling, especially as related to wider scale patterns in forest ecosystems within the east-Asia region.

Growth and Structural Properties of Fe Thin Films Electrodeposited on n-Si(111) (n-Si(111) 기판 위에 전기증착에 의한 Fe 박막의 성장과 구조적 특성)

  • Kim Hyun-Deok;Park Kyeong-Won;Lee Jong-Duk
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.10 no.9
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    • pp.1663-1670
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    • 2006
  • Single crystal Fe thin films were grown directly onto n-Si(111) substrates by pulsed electrodeposition. Cyclic Voltammogram(CV) indicated that the $Fe^{2+}/n-Si(111)$ interface shows a good diode behavior by forming a Schottky barrier. From Mott-Schottky (MS) relation, it is found that the flat-band potential of n-Si(111) substrate and equilibrium redox potential of $Fet^{2+}$ ions are -0.526V and -0.316V, respectively. The nucleation and growth kinetics at the initial reaction stages of Fe/n-Si(111) substraste was studied by current transients. Current transients measurements have indicated that the deposition process starts via instantaneous nucleation and 3D diffusion limited growth. After the more deposition, the deposition flux of Fe ions was saturated with increase of deposition time. from the as-deposited sample obtained using the potential pulse of 1.4V and 300Hz, it is found that Fe nuclei grows to three dimensional(3D) islands with the average size of about 100nm in early deposition stages. As the deposition time increases, the sizes of Fe nuclei increases progressively and by a coalescence of the nuclei, a continuous Fe films grow on the Si surface. In this case, the Fe films show a highly oriented columnar structure and x-ray diffraction patterns reveal that the phase ${\alpha}-Fe$ grows on the n-Si(111) substrates.

Investigation of thermodynamic analysis in GaN thick films gtowth (GaN 후막 증착의 열역학적 해석에 관한 연구)

  • 박범진;박진호;신무환
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.8 no.3
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    • pp.388-395
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    • 1998
  • This paper reports on a thermodynamic analysis for the GaN thick film growth by vapor phase epitaxy method. The thermodynamic calculation was performed using a chemical stoichiometric algorism. The simulation variables include the growth temperature in a range 400~1500 K, the gas ratios $(GaCl_3)/(GaCl_3+NH_3)$ and $(N_2)/(GaCl_3+NH_3)$. The theoretical calculation predicts that the growth temperature of GaN be in the lower range of 450~750 K than the experimental results. The difference in the growth temperature between the simulation and the experiments indicates that the vapor phase epitaxy of GaN is kinetically limited, presumably, due to the high activation energy of thin film growth.

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Late Fall Nitrogen Application and Turf Cover for Zoysiagrass (Zoysia japonica) Spring Green-up

  • Oh, Jun-Suk;Lee, Yu-Jin;Lee, Sang-Kook
    • Weed & Turfgrass Science
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    • v.4 no.4
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    • pp.383-389
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    • 2015
  • The use of zoysiagrass (Zoysia japonica Steud.) in the transition zone is limited because of a lack of cold hardiness although zoysiagrass has many advantages compared to other warm-season and cool-season grasses. Late-fall N fertilization is often applied for darker green color of turfgrass in early spring and more extensive root growth without rapid top growth. The objective of the study was to evaluate the effects of late fall N application and turf cover for zoysiagrass spring green-up. Clear polyvinyl chloride (PVC) film was used for turf cover. The amount of N applied were 5 and $10g\;N\;m^{-2}$ for the low and high N rate treatments, respectively. Covered zoysiagrass had greater turfgrass color and quality in early spring than non-covered zoysiagrass. The high N rate had 0.6 to 2.3 greater turfgrass quality than the low N rate on 7 of 9 rating dates. Slow-release N as late fall fertilization is more effective for turfgrass color and quality than fast-release N in spring. Turf cover could reduce the period of yellow zoysiagrass, and the earlier time of spring green-up could be advanced by increasing turfgrass quality and growth of zoysiagrass.

Short-Term Nutrient Enrichment Bioassays and Nutrient Limitation in Daechung Reservoir (대청호에서의 단기 영양염 첨가 실험 및 제한 영양염류 분석)

  • Lee, Sang-Jae;An, Kwang-Guk
    • Korean Journal of Ecology and Environment
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    • v.43 no.1
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    • pp.136-141
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    • 2010
  • In situ experiments of Nutrient Enrichment Bioassays (NEBs) were conducted in the field along with in the laboratory to determine which nutrient limited phytoplankton growth as a indicator of primary productivity. For the NEBs, the water was sampled using a polyethylene-lined container and dispensed into 6 L water tank in the laboratory. The control (C, no nutrient spike) and six treatments of phosphorus (P), 2-fold phosphorus (2P), 4-fold phosphorus (4P), nitrate nitrogen ($NO_3$-N), 2-fold nitrate nitrogen ($2NO_3$-N), and phosphorus and nitrate nitrogen (P+$NO_3$-N) were set up in the lacustrine zone near the dam site, Daechung Reservoir in September, 2009 and analyzed the diel changes of total nitrogen (TN), total phosphorus (TP), and chlorophyll-$\alpha$ (Chl-$\alpha$) in the cubitainers. The short-term NEBs showed that algal response in the treatments spiked phosphorus (P, 2P, and 4P) were significantly (p < 0.05) greater than the response in the control (C), and nitrogen-spike. Also, the response in 4P-treatment was greater than those in the P- and 2P-treatments. In contrast, there was no significant differences (p > 0.20) between the $NO_3$-N and $2NO_3$-N treatment. The outcomes of the NEBs suggest that phosphorus limited the phytoplankton growth and nitrogen was not limited in this system. Furthermore, in the N + P treatments, the response was minimum, compared to all other treatments and the control, indicating that even if the system is evidently P-limited system, when added the nitrogen, the response showed the inhibition. Also, > 95% of observed long-term TN:TP ratios in the ambient water showed > 17, which is the criteria of P-limitation, supporting the P-limitation in the system. Overall, these results suggest that phytoplankton biomass near the dam is a direct linear function of P-loading near the watershed, if the phosphorus pool is mainly dissolved fraction.

Effect of Fuel Injection Timing on Nitrous Oxide Emission from Diesel Engine (디젤엔진에서 연료 분사시기가 아산화질소에 미치는 영향)

  • Yoo, Dong-Hoon
    • Journal of Power System Engineering
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    • v.18 no.6
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    • pp.106-112
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    • 2014
  • The diesel engine, which has high compression ratio than other heat engines, has been using as the main power source of marine transport. Especially, since marine diesel engines offer better specific fuel consumption (SFC), it is environment-friendly compared to those used in other industries. However, attentio should be focused on emissions such as nitrous oxide ($N_2O$) which is generated from combustion of low-grade fuels. Because $N_2O$ in the atmosphere is very stable, the global warming potential (GWP) of $N_2O$ is 310 times as large as that of $CO_2$, and it becomes a source of secondary contamination after photo-degradation in the stratosphere. It has been hitherto noted on the $N_2O$ exhaust characteristics from stationary power plants and land transportations, but reports on $N_2O$ emission from the marine diesel engine are very limited. In this experimental study, a author investigated $N_2O$ emission characteristics by using changed diesel fuel components of nitrogen and sulfur concentration, assessed on the factors which affect $N_2O$ generation in combustion. The experimental results showed that $N_2O$ emission exhibited increasement with increasing of sulfur concentration in fuel. However, all kinds of nitrogen component additives used in experiment could not change $N_2O$ emission.