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Influences of Bulking Materials on Sustainable Livestock Mortality Composting (부자재 종류가 친환경적 사축퇴비화에 미치는 영향)

  • Won, Seung Gun;Park, Ji Young;Cho, Won Sil;Kwag, Jung Hoon;Choi, Dong Yoon;Ahn, Hee Kwon;Ra, Chang Six
    • Journal of Animal Science and Technology
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    • v.55 no.5
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    • pp.483-488
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    • 2013
  • To develop a sustainable composting method for livestock mortality, a natural aeration-composting process was designed and the influences of bulking materials on the mortality composting process were studied. Bulking materials (e.g., compost, swine manure, sawdust, and rice husks), easily supplied at the scene of an animal mortality outbreak, were tested in this research. A lab-scale composting system (W34 ${\times}$ L60 ${\times}$ H26 cm) was made using 100 mm styrofoam, and natural aeration was achieved through pipes installed on the bottom of the system. Four treatments were designed (compost, compost + swine feces, sawdust, and rice husks treatment groups) and all experiments were done in triplicates. During composting for 40 days, no leachate was observed in compost and sawdust treatment groups, whereas 18 and 8.2 ml leachate/kg-mortality was emitted from the compost + feces and rice husks treatment groups, respectively. Dimethyl disulfide (DMDS) emission during the composting was very low in all treatment groups, possibly due to the bio-filtering function of the compost cover layer on the pile. The mortality degradability in compost, compost + feces, sawdust, and rice husks groups was 25.3, 25.8, 13.5, and 14.5%, respectively, showing significantly higher levels in compost and compost + feces groups (p<0.05). Also, only the compost + feces group produced enough heat (over $55^{\circ}C$) and lasted for 7 days, indicating that bio-security cannot be guaranteed without feces supplementation.

Inhibitory Substance on the Snake Venoms Produced by Penicillium sp. (사독의 조해물질에 관한 연구)

  • Seu, Jung-Hwn;Yi, Dong-Heui
    • Microbiology and Biotechnology Letters
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    • v.7 no.2
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    • pp.75-89
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    • 1979
  • One strain of Penicillium sp. (175-66-B), isolated from soil, was able to produce a substance that has a strong inibition activity against the Agkistrodon and Trimeresurus venoms. In this experiment, the chemical and biological properties of the sample were investigated. As an inhibitory substance, it was effective to the proteinase, hemorrhagic and lethal factors of Agkistrodon and Trimeresurus venoms, and also effective to several fractions of the proteinases and hemorrhagic factors of Agkistrodon halys blomhoffi venom. Moreover, in the addition of prednisotone, it was more effective for the cure of the mouse envenomated with the venom amount of two fold of MLD$_{100}$. This substance was very stable to the acid, alkali and heat. Its melting point was high enough to sublime at 222$^{\circ}C$ without any decomposition. This sample was easily dissolved only in hot water, but not in several organic solvents except for a little dissolution in elate. It did not have the chelating activity. It had very strong specificity to the snake venoms. but its activity was depressed by the addition of zinc or cupric salts. This sample had no acute toxicity to the mouse. Its chemical formula was $C_{16}$ $H_{12}$$N_2$ $O_{10}$ with the molecular weight of about 392. It has two epoxy groups and four carboxyl radicals, but amino, nitrite and nitrate radicals, unsaturated bonds and aromatic ring were not detected. Theuchemical configuration of this sample was suggested to be;

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Naturally Collection and Development until Yolk Absorption of Domestic Walleye Pollock Theragra chalcogramma Fertilized Eggs and Larvae (국내 명태 Theragra chalcogramma 자연채란과 난황흡수까지의 난 발생)

  • Seo, Joo-young;Kwon, O-Nam
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.18 no.1
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    • pp.49-54
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    • 2017
  • We collected and reared Theragra chalcogramma walleye pollock brood-stock for use in natural spawning tests and undertook to obtain domestic pollock via fertilized egg capture, development of fertilized eggs, and absorption of yolk sac after hatching. Whole pollock were caught with trammel and set nets and immediately placed in a deep-sea water tank. Adults were the most common pollock age group (43.0%; n = 86) among the 254 pollock captured in March 2014 with 57.9% (n = 147) being captured off Southern Gosung, Korea. The main spawning period of pollock is February (spawning phase of 91% of pollock). From the deep-sea tank, we collected 1640 mL of naturally fertilized eggs (~820,000 eggs) from 12 spawning events occurring between February 4 and 22 2015. The floating/ live eggs were maintained in deep-sea water tanks at $5.5{\pm}0.2^{\circ}C$. Egg size was $1.5{\pm}0.03mm$. Six hours after fertilization the eggs were at the 2 cell stage, and the eggs hatched approximately 340 hours after collection. At hatching, larval length and yolk sac area were $5.2{\pm}0.25mm$ and $9.5{\pm}1.00mm^2$ (100%), respectively. Four days after hatching, the yolk sac area was $2.2{\pm}0.53mm^2$ ($23.1{\pm}5.55%$). This is the first report of collection of naturally fertilized eggs from pollock and their subsequent hatching while held in an indoor deep-sea water tank. The results suggest that such collection could assist in the recovery of pollock resources and the possibility of domestic rearing of cultivated larvae.

Physiological Characteristics of Cardiopulmonary Function, Electromyogram and Blood Chemistry in Athletic High School Students (고등학교(高等學校) 운동선수(運動選手)의 심폐기능(心肺機能), 근전도(筋電圖) 및 혈액성분(血液成分)의 특성(特性))

  • Choo, Young-Eun;Lee, Won-Jung;Park, Jae-Sik;Kim, Hyeong-Jin;Hwang, Soo-Kwan
    • The Korean Journal of Physiology
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    • v.18 no.1
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    • pp.67-79
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    • 1984
  • In an effort to elucidate the physiological characteristics in cardiopulmonary function, electromyogram(EMG), and blood chemistry in athletic high school students, an analysis of electrocardiogram(ECG) and EMG, pulmonary function test, venous blood gas analysis($Pvo_2$ and$Pvco_2$), and measurement of heart rate, blood pressure, respiratory rate, blood glucose and blood lactate were made for 16 to 19 year-old high school students who were divided into athletic (n=19) and non-athletic (n=20) group. The results obtained are summarized as follows. 1) ECG intervals in athletes were longer than in non-athletes, and the difference was significant in R-R, Q-T and T-P intervals. Resting heart rate in athletes was 56.3/min showing a bradycardia compared with 79.8/min in non-athletes. Amplitudes of R and T waves in lead $V_5$ were significantly higher than in non-athletes. 2) Pulmonary function parameters in athletes showed higher values than in non-athletes. Parameters which showed significant differences were FEV 0.5, PEF, FEF 25%, PIF and FEF $200{\sim}1.200\;ml. 3) Heart rate, blood pressure, and respiratory rate after exercise were significantly elevated from resting values. Heart rate and respiratory rate showed greater increase in non-athletes, while blood pressure showed greater increase in athletes. 4) $Pvo_2$ was lowered ana $Pvco_2$ was elevated after exercise, and there was no significant difference between two groups. 5) Blood glucose and lactate levels were elevated after exercise. The difference was significant in blood lactate, and was greater in non-athletes. 6) EMG amplitude was steadily increased with increasing load of exercise, and the increase was greater in athletes than in non-athletes.

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A Study on Stability of Inorganic Binder for Application as Conservation Material for Stone Monuments (석조문화재 보수물질로의 적용을 위한 무기질 바인더 안정성 연구)

  • Kim, Dae Sik;Do, Jin Young;Cho, Hyen Goo
    • Journal of the Mineralogical Society of Korea
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    • v.27 no.4
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    • pp.251-262
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    • 2014
  • Inorganic binders were studied in order to apply a conservation material for stone monument. A pure inorganic binder and 3 species of inorganic binder which contain additives on the basis of a pure inorganic binder were selected as test samples. Through the application of inorganic binders on Geochang granite investigate their influences on stone. pH 4.0 and 5.6 acid solution, respectively were manufactured on the basis of the acidity of domestic rainfall. Alkaline water with pH 8.0 and deionized water with pH 6.85 were prepared as control group. Changes in weights of inorganic binders were not definite according to the acidity of water while weight losses in inorganic binder type were greater after reaction with the water. The compressive strengths of pure inorganic binder was largest before the test but its decrease rate were larger after reaction with the water. Water absorption rate of inorganic binders are 6.72 to $12.44kg/m^2{\cdot}t^{1/2}$ after reaction with the water. Such high absorption was considered that it forced water to move deep into inorganic binder and made the components of inorganic binder dissolve. Acidities of the water of pH 4.0, 5.6, 6.85 and 8.0, respectively were changed to pH 9.0-10.0 after reaction with the inorganic binders. Ion concentrations in the water changed after reaction with the inorganic binders and $Mg^{2+}$, and $K^+$ significantly increased, dissolved from the binder. The high concentration of ions detected showed that the binder reacted with water and formed white salts with high solubility such as $MgSO_4{\cdot}nH_2O$, $KNO_3$. Ion concentrations significantly decreased from the binder after treatment with consolidant and water repellent.

Changes of the Clinicopathological Characteristics and Survival Rates of Gastric Cancer with Gastrectomy - 1990s vs early 2000s (위절제 위암 환자의 임상병리학적 특성과 생존율의 변화: 1990년대와 2000년대 초기의 비교)

  • Sim, Young-Kwan;Kim, Chan-Young;Jeong, Yeon-Jun;Kim, Jong-Hun;Hwang, Yong;Yang, Doo-Hyun
    • Journal of Gastric Cancer
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    • v.9 no.4
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    • pp.200-206
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    • 2009
  • Purpose: The incidence of upper gastric cancer and especially the diffuse type have increased in western countries. The aim this study was to investigate the chronologic changes of the clinicopathological features and survival rates of Korean upper gastric cancer patients. Materials and Methods: 1,638 gastric cancer patients who underwent gastrectomy were included in this study and they were divided into two groups; the 1990's (1991~1999, n=987) and the early 2000's (2000~2003, n=651). We evaluated the differences of the clinicopathologic features and the factors that affected the survival rates by univariative and multivariative analysis. Results: The older age (>60) patients increased from 42.7% to 50.7% respectively. Being overweight (body mass index$\geq$23) also increased from 31.5% to 43.2%. For the pathology, the incidence of stage Ia gastric cancer increased (29.8% to 44.5%) and the incidence of stage IV gastric cancer decreased (23.5% to 11.8%). Yet there was no difference according to the WHO classification, Lauren's classification and the location of tumor between the groups. The 5 year survival rates increased 67.7% to 83.7%, according to the group. Multivariative analysis showed that the odd ratios of the early 2000s was 0.715 (95% CI; 0.555~0.921) as compared to that of the 1990s. Conclusion: There were no changes of the clinicopathologic features, like the pattern in western countries, although the incidence early gastric cancer, old age patients and overweight patients increased. The survival rate of early 2000s was better that that of the 1990s.

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IN VITRO STUDY OF CARIOGENIC POTENTIAL OF INFANT CONFECTIONARIES (유아용 과자의 우식유발능에 관한 생체 외 연구)

  • Song, Ji-Hyon;Lee, Kwang-Hee;Kim, Dae-Eup;Ra, Ji-Young;Lee, Dong-Jin;An, So-Youn
    • Journal of the korean academy of Pediatric Dentistry
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    • v.35 no.1
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    • pp.39-46
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    • 2008
  • The purpose of this study was to assess the cariogenic potential of infant confectionaries. In vitro, as compaired with 10% sucrose solution and whole bovine milk. Buffering capability were determined by amount of 0.1N lactic acid consumed to titrate the 50ml specimen solutions to pH 4.0. The pH of the specimen solution inoculated by streptococcus mutans was measured by pH meter and the surface microhardness tester, before and after 48 hours incubation. The buffering capacity of infant confectionaries was higher than that of sucrose solution and lower than that of milk, and there were significant difference between infant confectionaries(p<0.05). The pH of infant confectionaries after 48 hours incubation was similar to 10% sucrose solution, and there were significant difference between infant confectionaries and milk(p<0.05). The microhardness change of primary tooth enamel of infant confectionaries group after 48 hours incubation was similar to that of 10% sucrose solution, and there were significant difference between infant confectionaries and milk(p<0.05). In conclusion, infant confectionaries seemed to have the ability to cause dental caries in primary teeth, and there were significant differences of cariogenic potential among infant confectionaries. Cooperative efforts of dentistry and manufacturers to reduce the cariogenic potential of infant confectionaries would be necessary to prevent the early childhood caries in children.

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Quality Characteristics and Anti-Diabetic Effect of Yacon Vinegar (야콘 식초의 품질특성 및 항당뇨 효과)

  • Lee, Mi-Kyung;Choi, Sa-Ra;Lee, Jin;Choi, Yun-Hong;Lee, Ju-Hye;Park, Kyung-Uk;Kwon, Seung-Hyek;Seo, Kwon-Il
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.41 no.1
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    • pp.79-86
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    • 2012
  • This study was performed to investigate the physiochemical properties and anti-diabetic effect of yacon vinegar by two-step fermentation. Yacon was matured at room temperature for 20 days. The sugar content of yacon juice prepared from mature yacon was approximately $14^{\circ}Brix$. In the first stage, yacon wine was produced from the juice at $28^{\circ}C$ for 6 days. In the second stage, acetic acid fermentation was conducted at $30^{\circ}C$ and 200 rpm for 6 days to produce yacon vinegar with 4.75% acidity. The major free sugars of yacon vinegar were glucose and fructose at 2,072.12 mg% and 463.95 mg%, respectively. The acetic acid content was the highest of the major organic acids at 3,881.44 mg%. The total free amino acid content was 62.88 mg% with the main free amino acids being proline, ${\gamma}$-amino-n-butyric acid and ornithine. The major minerals of yacon vinegar were Ca, K and Mg. The in vivo anti-diabetic activity of yacon vinegar was investigated in high-fat diet (HFD)/streptozotocin (STZ)-induced diabetic mice. Diabetic mice were administered orally with 10% yacon juice and two yacon vinegars (5% and 10%) at a dose of 7 mL/kg body weight once per day for 4 weeks. Five% yacon vinegar improved the fasting blood glucose levels and glucose tolerance test significantly compared to the diabetic control group (p<0.05). Yacon vinegar increased the pancreatic C-peptide concentration in a dose-dependent manner. These results show that 5% yacon vinegar has a more potent effect on ameliorating hyperglycemia than 10% yacon juice.

Nationwide Survey of Laparoscopic Gastric Surgery in Korea, 2004 (2004년 전국 복강경 위 수술 현황)

  • Kim, H.H.;Kim, K.H.;Kim, D.H.;Kim, M.C.;Kim, B.S.;Kim, Y.W.;Kim, Y.I.;Kim, Y.H.;Kim, W.;Kim, W.W.;Kim, J.J.;Kim, T.B.;Ryu, S.Y.
    • Journal of Gastric Cancer
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    • v.5 no.4 s.20
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    • pp.295-303
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    • 2005
  • The Korean laparoscopic Gastrointestinal Surgery Study Group made a survey of laparoscopic gastric surgeries which were performed in Korea during 2004. Thirty-eight surgeons from 36 Institutions responded to the questionnaires. One thousand eighty-nine laparoscopic gastric operations were performed during 2004. The cumulative number from 1995 to 2004 was about 2,386. Seven hundred fifty-four operations for a gastric adenocarcinoma were performed during 2004 which is almost two times the number performed during 2003. Laparoscopic radical procedures, such as a laparoscopy-assisted distal gastrectomy or total gastrectomy (LADG and LATG) have increased rapidly since 2001 (55 cases in 2001, 150 cases in 2002, 364 cases in 2003 and 738 cases in 2004). Especially, laparoscopic total gastrectomies were explosively adopted last year (20 cases in 2003 and 112 cases in 2004). However, laparoscopic function-preserving gastrectomies, which included one laparoscopy-assisted pylorus-preserving gastrectomy and laparoscopy-assisted proximal gastrectomy, are rarely performed at this time. One hundred forty-two wedge resections for a gastric submucosal tumor were performed during 2004. Hand-assisted laparoscopic surgery (HALS) was performed in 39 cases in 2001, 55 in 2002, and 49 in 2003; however, only 5 such surgeries were performed during 2004. In 2003, laparoscopic bariatric surgery began, and during 2004, 49 operations were performed. In terms of indications of laparoscopic gastric surgery for adenocarcinoma, 19 surgeons performed a LADG only for a T1 lesion, and 7 surgeons extended their indications to T2N0 lesions. In the near future, laparoscopic procedures for gastric cancer will be widely adopted in Korea if the medical-insurance obstacle is overcome, and the long-term survival results are verified.

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Studies on the Post-hatching Development of Sertoli and Leydig Cells in the Testis of Korean Native Chickens (한국재래 닭 고환 지지세포와 간질세포의 부화 후 발달에 관한 연구)

  • Tae H. J.;Jang B. G.;Choi C. H.;Park Y. J.;Yang H. H.;Kim I. S.
    • Korean Journal of Poultry Science
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    • v.32 no.2
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    • pp.125-133
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    • 2005
  • Morphometric changes in testicular Sertoli and Leydig cells from hatching to adulthood were studied using Korean native chickens of 1, 2, 4, 6, 8, 10, 12, 14, 16, 18, 21, 24, 28, 32, 44, 52 and 64 weeks (n=13 chickens per group) of age. The objective of this study was to understand the developmental phase of the Sertoli and Leydig cells with age. Testis of chickens was fixed by whole body perfusion using a fixative containing $2.5\%$ glutaraldehyde in cacodylate buffer, processed and embedded in Epon-araldite. Using 1 Um sections stained with methylene blue-azure II, qualitative and quantitative (stereological) morphological studies were performed. The average volume of a testis of 1 week old Korean native chickens was determined as $0.148\;cm^3$ and the parameter increased linearly from 1 week to 21 weeks days $(28.86\;cm^3)$, and did not change from 21 weeks to 64 weeks. The volume density of the seminiferous tubules increased with age from $32.6\%$ at week 1 to $92.89\%$ at week 64. The volume density of the interstitium represents $67.4\%$ of the testicular parenchyma at week 1. This proportion progressively diminished during development to reach a value of $7.11\%$ at week 64. The volume density of the Leydig cells decreased almost linearly from 1 week $(4.9\%)$ to 14 weeks $(1.7\%)$ and remained unchanged thereafter. In contrast, the Sertoli cells occupied a volume density of $3.4\%$ at week 1, increased progressively up to 18 weeks of age $(10.79\%)$ and remained unchanged thereafter. The absolute volume of the Leydig and Sertoli cells per testis increased significantly from week 1 to week 21 but did not change significantly from week 24 to week 64. The number of Leydig cells per testis increased almost linearly from 1 week to 21 weeks, remained high and unchanged with advancing age. The number of Sertoli cells per testis increased gradually with age from 1 week to 14 weeks and remained unchanged thereafter.