• Title/Summary/Keyword: N-generations

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Isolation and characteristics of yellow-pigment producing mutants of Monascus anka. (Monascus anka로부터 황색소 생성 변이주의 분리 및 특성)

  • 이호재;이형주
    • Microbiology and Biotechnology Letters
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    • v.30 no.2
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    • pp.111-115
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    • 2002
  • To produce yellow pigment selectively, mutants were induced from Monascus anka Nakazawa et Sato IFO 4478 (KCCM 11832 strain), and their characteristics were evaluated. Five kinds of auxotrophic mutants which required amino acids for growth and pigmentation, were isolated through a series of mutagenic treatments. Especially, asparagine auxotroph Y7 produced high ratio of yellow pigment. This mutant showed all the morphological characteristics of Monascuceae but the shape of colony and the diameter of conidia. Mutant Y7 was propagated by sexual reproduction more often than asexual reproduction, which could be effective in production of pigments. Yellow pigment produced extracellularly by the mutant Y7 was more soluble in polar solvents such as ethanol and water than in nonpolar solvents. Its productivity of yellow pigment was 2.2 times higher in the mutant Y7 than in parents. In addition, its yellow pigment showed characteristics of maximum absorption at 373 nm. Moreover, the hue of pigment produced by the mutant Y7 was bright yellow, and it was stable through the subculture over 10 generations.

Studies on the Tricholyga sorbillans Wiedmann (다화성잠저승(Tricholyga sorbillans Wiedmann)에 관한 연구)

  • 김낙정;박광의;강석권
    • Journal of Sericultural and Entomological Science
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    • v.8
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    • pp.51-62
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    • 1968
  • This study was carried out to investigate the life cycle and the damage of Tricholyga sorbillans Wiedmann to silkworms(Bombyx mori L.). The results are summarized as follows. 1. This insect has 5 or 6 generations per year in Suwon area. 2. The egg, larval, and pupal stage are about 2, 8 and 18 days respectively. 3. The weather conditions considerably affect the life cycle, that is, the 3 rd generation is the shortest of all, lasting for 21 days and l4 hours, and the 5th generation is the longest for about 40 days. 4. The larvae come out of silkworm bodies, burrowing into the ground 3.5cm to 10.5cm in depth before changing into the pupae. 5. This insect does not overwinter as pupa, but seems to do as larva, parasiting on the field insects. 6. The damage of Tricholyga sorbillans Wiedmann to silkworm was 27.9%, it is so high that the silk worms could not be reared outside without a reasonable control method. 7. When the full-grown silkworm is parasited by one egg of these insects, there is not only enough possibility of spinning. but also without any effects of their larvae on the cocoon quality. 8. The damage of Tricholyga sorbillans Wiedmann throughout the country covers 6.36% in spring, 4.09% in fall, and 5.25% yearly mean.

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Aspects of Emotional Customs by the N-po Generation (N포세대의 감정 풍속도)

  • Seo, Yeon-Ju
    • Journal of Popular Narrative
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    • v.25 no.1
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    • pp.55-85
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    • 2019
  • In this article, we examine the real meaning behind the stories in which the N-po Generation (Millennial Generation) is depicted, through the observation of entertainment programs, TV series, and movies. This could be an opportunity to investigate the aspects of emotional customs of our era, which have been described by television media as portraying the complex and multifaceted reality in the most mundane and popular manner while influencing the public. Problems with youth unemployment, the polarization of life, and instability are not only global issues but situations that specifically occur in South Korea. It is thus vital to pay attention to the inner side of the N-po Generation who enjoy Sohwakhaeng (small but certain happiness) by eating alone as the placebo effect of this tough reality. This is an agenda that should be viewed as a problem in the fundamental design of South Korean society. The consciousness of the problem shown in the TV series has been drawing attention. The TV series Because depicts a love narrative that concentrates on emotions in a relationship that started between housemates due to poverty and housing problems, leading to marriage. Thus, the TV series persuasively dramatized 'confluent love' in the N-po Generation. In the movie , Miso can be regarded as a symbol that represents the emergence of a new generation of cultural sensitivity. There is a suggestion in the sequence of that identifies the pursuit of taste with the discovery of identity. The TV series is a growth narrative that deals heavily with youth unemployment, temporary workers, fragmented families, and dating violence. The housemates in find emotional stability through interaction with each other, and courageously approach their individual problems. In the process, images of women, who are empathetic towards others and are willing to jointly solve their problems, are calmly depicted to reveal a story of growth revolving around a ground emotional community. The current problem that South Korean society should contemplate is how to be fully human beyond mere survival, and how to further seek the conditions of human existence. In that sense, what we should pursue is a notion of 'publicness', which can put several generations together. Because of the reality that confliction between generations must be triggered, in order to make a passage of sympathizing, mass media's sensitivity training becomes more important. This may be the duty of mass media.

Stewardship, Stockmanship and Sustainability in Animal Agriculture

  • Szucs, E.;Geers, R.;Sossidou, E.N.
    • Asian-Australasian Journal of Animal Sciences
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    • v.22 no.9
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    • pp.1334-1340
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    • 2009
  • Sufficient food supply for all humans was, is, and will remain one of the main priorities for mankind. The choice between food from crops or animals is related to philosophical, religious and ethical, but also cultural and economical, values. However, the concept of sustainable agriculture takes into account the organization of food supply through future generations. Not only quantity, but also quality is important, especially in relation to food safety and the method of production. Specifically, the aspect of animal welfare is becoming increasingly important with the focus on stewardship and stockmanship, i.e. responsibility of humans for their animals. In the future, implications for sustainability in animal production may be of more concern to stewardship paired by stockmanship, responsibility, consciousness and morality. The moral as a basic concept of sustainable agriculture is to maintain continuous development in harmony with nature to meet requirements in the world for living creatures including human beings to live in and steward. The objective of this paper is to discuss the above issues from different viewpoints on sustainable food supply, increasing food consumption and environmental protection.

Screening of BCL-2 associated X protein gene polymorphism associated with scrotal hernia in domesticated swine using polymerase chain reaction-restriction fragment length polymorphism

  • Manalaysay, Jessica G.;Antonio, Nathaniel D.;Apilado, Ralph Lorenz R.;Bambico, Joseph F.;Mingala, Claro N.
    • Asian-Australasian Journal of Animal Sciences
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    • v.30 no.2
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    • pp.262-266
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    • 2017
  • Objective: This study was conducted to screen scrotal hernia in domesticated swine from selected breeders in the Philippines. This defect is associated with a cytosine to thymine mutation in the BCL-2 associated X protein (BAX) gene of swine. Methods: Genetic screening was done by DNA extraction followed by amplification and digestion using polymerase chain reaction-restriction fragment length polymorphism, amplifying the 416 bp region of the BAX gene that was subjected to digestion using the Ear I enzyme. Sequencing was also conducted to validate the results. Results: Results revealed that out of 538 samples tested, 411 (76.4%) of the samples were found to be normal whereas the remaining were carriers of the mutation in which 80 (14.9%) were heterozygous mutants and 47 (8.7%) were homozygous mutants. Pietrain breed was found to have the highest incidence. Conclusion: Having a scrotal hernia eliminates the chances of using the boar as a breeder stock because the following generations arising from it would most likely exhibit herniation. It is therefore advised to establish a genetic screening method for Scrotal Hernia in the Philippines to eliminate the negative gene from the herd.

An Analysis on Success and Failure Factors of Domestic and Foreign Businesses Using Social Network Service (SNS를 활용한 국내외 비즈니스의 성공요인과 실패요인 분석)

  • Kim, Chang Su;Kim, Tae Hyun;Park, Gyeong Won
    • Journal of Korea Society of Digital Industry and Information Management
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    • v.10 no.2
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    • pp.229-240
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    • 2014
  • While the digital environment continues to diffuse rapidly, SNS has been playing important roles in business growth. This study analyzes businesses that have used social network services (SNS) in order to more successfully manage companies by utilizing SNS and to suggest the proper steps In order to accomplish this, major domestic and foreign business cases were selected in order to investigate factors of success and failure for each of them. The analysis results show that the companies that utilized SNS successfully understood the characteristics of SNS and constantly tried creative marketing. On the other hand, enterprises that failed to use SNS properly showed low understanding of SNS and abnormal ways of marketing. Therefore, this research suggests the following conclusion. First of all, sufficient knowledge and understanding of the characteristics of SNS are necessary in order to properly utilize SNS. Next, it is imperative to use SNS not only as a tool to communicate with customers, but also to understand the generations that use SNS. Finally, it would be best to implement a more open wireless internet mobile environment for more active use of SNS.

A Study on the Thermal Characteristics of a High Speed Spindle according to the Cooling Existence of Rear Part and the Cooling Conditions (고속주축의 냉각조건과 후반부 냉각 유무에 따른 열특성 연구)

  • Choi, Dae-Bong;Kim, Soo-Tae;Lee, Seog-Jun;Kim, Chang-Yong
    • Journal of the Korean Society of Manufacturing Process Engineers
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    • v.11 no.1
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    • pp.50-55
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    • 2012
  • The important problem in high speed spindle is to reduce and minimize the thermal effect by motor and bearing. This paper presents the thermal characteristic analysis for a high speed spindle with and without cooling at the rear part, considering the viscosity and the flow rate of cooling oil. A high speed spindle is composed of angular contact ceramic ball bearings, high speed built-in motor, oil jacket cooling and so on. The thermal analyses of high speed spindle need to minimize the thermal effect and maximize the cooling effect and they are carried out under the various cooling conditions. Heat generations of the bearing and the high speed motor are estimated from the theoretical and experimental data. This result can be applied to the design and manufacture of a high speed motor spindle.

시스템 다이내믹스를 이용한 폐기물 관리정책에 관한 연구

  • 문태훈;서원석
    • Proceedings of the Korean System Dynamics Society
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    • 2000.07a
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    • pp.65-102
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    • 2000
  • In 1987, The Brundtland Report defined a sustainable development as the development that meets the needs of the present without compromising the ability of future generations to meet their own needs. This is the concept that we should cling to, to preserve natural resources of the present for the future generation. With this concept in mind, this paper criticized that the current waste management policy has been neglecting resource preservation aspect of waste management policy while giving too much emphasis on reducing waste generation. Thus, the purpose of this paper is to analyze how waste management policy can affect preservation of natural resources, and to investigate policy alternatives that can reduce both the volume of waste generated and the amount of natural resources that need to be consumed in the production process. The study was conducted based on the literature survey and system dynamics simulation. Borrowing Randers and Meadows's solid waste model with some modification of parameter and variables, this paper simulate several policy alternatives to figure out the most effective waste management policy set that can meet the need of the present without compromising the ability of the future generation to meet their own needs. Main concern was how to link waste management policy with resource management policy that can lead to minimization of waste generation and resource consumption.

Performance of High Strength Self-Compacting Concrete Beams under Different Modes of Failure

  • Harkouss, Raya Hassan;Hamad, Bilal Salim
    • International Journal of Concrete Structures and Materials
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    • v.9 no.1
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    • pp.69-88
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    • 2015
  • Self-consolidating concrete (SCC) is a stable and cohesive high consistency concrete mix with enhanced filling ability properties that reduce the need for mechanical compaction. Limited standards and specifications have been reported in the literature on the structural behavior of reinforced self-compacting concrete elements. The significance of the research presented in this paper stems from the need to investigate the effect of enhanced fluidity of SCC on the structural behavior of high strength self-consolidating reinforced concrete beams. To meet the objectives of this research, twelve reinforced concrete beams were prepared with two different generations of superplasticizers and designed to exhibit flexure, shear, or bond splitting failure. The compared beams were identical except for the type of superplasticizer being used (second generation sulphonated-based superplasticizer or third generation polycarboxylate-based superplasticizer). The outcomes of the experimental work revealed comparable resistance of beam specimens made with self-compacting (SCC) and conventional vibrated concrete (VC). The dissimilarities in the experimental values between the SCC and the control VC beams were not major, leading to the conclusion that the high flowability of SCC has little effect on the flexural, shear and bond strengths of concrete members.

Thermal Characteristics and Frequency Analysis of a High Speed Spindle for Small Tapping Center (소형 태핑센터 주축의 열특성 및 주파수 분석)

  • Choi, Dae-Bong;Kim, Soo-Tae;Ro, Seung-Kook;Cho, Hyun-Taek
    • Journal of the Korean Society of Manufacturing Process Engineers
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    • v.11 no.2
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    • pp.105-111
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    • 2012
  • High speed machining is the core technology that influences the performance of machine tools, and the high speed motor spindle is widely used for the high speed machine tools. The important problem in high speed spindle is to minimize the thermal effect by motor and bearing and frequency effect. This paper presents the thermal characteristic analysis and frequency experiment for a high speed spindle considering the flow rate of cooling oil. A high speed spindle is composed of angular contact ceramic ball bearings, high speed built-in motor, oil cooling jacket and so on. The thermal analyses of high speed spindle need to minimize the thermal effect and maximize the cooling effect and they are carried out under the various cooling conditions. Heat generations of the bearing and the high speed motor are estimated from the theoretical and experimental data. To find out the characteristic of vibration, the high speed spindle is excited in operational range. This result can be applied to the design and manufacture of a high speed tapping spindle.