• 한국동물위생학회지
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• 제31권4호
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• pp.493-496
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• 2008

Brucella spp. are small, non-motile Gram-negative coccobacilli known to cause disease in a number of vertebrate species including humans and brucellosis is one of the world's major zoonoses, alongside bovine tuberculosis and rabies. There are about 33.55 million goats and 1.16 million sheep in Bangladesh. The sheep and goats can significantly play an important role in the economic well being of the resource-poor farmer in Bangladesh. Sexually matured 362 female small ruminants(300 goats and 62 sheep) were examined. Approximately 3-5 ml of blood was collected from the jugular vein of each animal and sera samples were prepared. Samples were then tested for brucellosis by using Rose Bengal test(RBT), plate agglutination test(PAT) and tube agglutination test(TAT). Among 362 small ruminants, irrespective of species(sheep or goat), diagnosed highest in TAT, 2.21%(n=8) and lowest both by RBT & PAT, 1.93%(n=7) and it is concluded that TAT is superior than RBT and PAT.

• Journal of Microbiology and Biotechnology
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• 제25권11호
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• pp.1908-1919
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• 2015

This work investigated the potential of curcumin (CCM) and (-)-epigallocatechin gallate (EGCG) to inhibit N-acyl homoserine lactone (AHL)-mediated biofilm formation in gram-negative bacteria from membrane bioreactor (MBR) activated sludge. The minimum inhibitory concentrations (MICs) of CCM alone against all the tested bacteria were 200-350 μg/ml, whereas those for EGCG were 300-600 μg/ml. Biofilm formation at one-half MICs indicated that CCM and EGCG alone respectively inhibited 52-68% and 59-78% of biofilm formation among all the tested bacteria. However, their combination resulted in 95-99% of biofilm reduction. Quorum sensing inhibition (QSI) assay with known biosensor strains demonstrated that CCM inhibited the expression of C4 and C6 homoserine lactones (HSLs)-mediated phenotypes, whereas EGCG inhibited C4, C6, and C10 HSLs-based phenotypes. The Center for Disease Control biofilm reactor containing a multispecies culture of nine bacteria with one-half MIC of CCM (150 μg/ml) and EGCG (275 μg/ml) showed 17 and 14 μg/cm² of extracellular polymeric substances (EPS) on polyvinylidene fluoride membrane surface, whereas their combination (100 μg/ml of each) exhibited much lower EPS content (3 μg/cm²). Confocal laser scanning microscopy observations also illustrated that the combination of compounds tremendously reduced the biofilm thickness. The combined effect of CCM with EGCG clearly reveals for the first time the enhanced inhibition of AHL-mediated biofilm formation in bacteria from activated sludge. Thus, such combined natural QSI approach could be used for the inhibition of membrane biofouling in MBRs treating wastewaters.

• Journal of Microbiology and Biotechnology
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• 제18권2호
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• pp.219-227
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• 2008

The free-living photoheterotrophic Gram-negative bacterium Rhodobacter sphaeroides possesses a quorum-sensing (QS) regulatory system mediated by CerR-CerI, a member of the LuxR-LuxI family. To identify the genes affected by the regulatory system, random lacZ fusions were generated in the genome of R. sphaeroides strain 2.4.1 using a promoter-trapping vector, pSG2. About 20,000 clones were screened and 23 showed a significantly different level of ${\beta}$-gal activities upon the addition of synthetic 7,8-cis-N-tetradecenoyl-homoserine lactone (RAI). Among these 23 clones, the clone showing the highest level of induction was selected for further study, where about a ten-fold increase of ${\beta}$-gal activity was exhibited in the presence of RAI and induction was shown to be required for cerR. In this clone, the lacZ reporter was inserted in a putative gene that exhibited a low homology with catD. A genetic analysis showed that the expression of the catD homolog was initiated from a promoter of another gene present upstream of the catD. This upstream gene showed a strong homology with luxR and hence was named qsrR (quorum-sensing regulation regulator). A comparison of the total protein expression profiles for the wild-type cells and qsrR-null mutant cells using two-dimensional gel electrophoresis and a MALDI-TOF analysis allowed the identification of sets of genes modulated by the luxR homolog.

• Journal of Microbiology and Biotechnology
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• 제20권7호
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• pp.1061-1068
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• 2010

The biological synthesis of nanoparticles has gained considerable attention in view of their excellent biocompatibility and low toxicity. We isolated and purified rhamnolipids from Pseudomonas aeruginosa strain BS-161R, and these purified rhamnolipids were used to synthesize silver nanoparticles. The purified rhamnolipids were further characterized and the structure was elucidated based on one- and two-dimensional $^1H$ and $^{13}C$ NMR, FT-IR, and HR-MS spectral data. Purified rhamnolipids in a pseudoternary system of n-heptane and water system along with n-butanol as a cosurfactant were added to the aqueous solutions of silver nitrate and sodium borohydride to form reverse micelles. When these micelles were mixed, they resulted in the rapid formation of silver nanoparticles. The synthesized nanoparticles were characterized by UV-Visible spectroscopy, transmission electron microscopy, and energy dispersive X-ray spectroscopy (EDS). The nanoparticles formed had a sharp adsorption peak at 410 nm, which is characteristic of surface plasmon resonance of the silver nanoparticles. The nanoparticles were monodispersed, with an average particle size of 15.1 nm (${\sigma}={\pm}5.82$ nm), and spherical in shape. The EDS analysis revealed the presence of elemental silver signal in the synthesized nanoparticles. The formed silver nanoparticles exhibited good antibiotic activity against both Grampositive and Gram-negative pathogens and Candida albicans, suggesting their broad-spectrum antimicrobial activity.

• 한국도서관정보학회지
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• 제46권1호
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• pp.321-344
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• 2015

이 논문은 여러가지 자료를 결합해 어떤 주제에 관한 의견이 실려있는 블로그를 찾는 융합적 의견탐지방법을 소개한다. 주제에 관한 의견이 담긴 블로그를 찾기위해 이 연구는 기존의 IR 방법으로 주제에 관한 블로그를 검색한 후 여러가지 의견탐지 방법을 합산한 의견점수로 검색결과의 순위를 조정하는 방법을 쓴다. 의견탐지 모듈의 주요 구성 요소는 의견이 실려있는 블로그에 자주 나오는 단어들을 활용한 고빈도 모듈, 강한 감정을 표현하는 희귀 한 용어들을 (e.g., "sooo good") 활용한 저빈도 모듈, "I"와 "you"에 묶인 n-gram을 (e.g., I believe, You will love) 활용한 IU모듈, 윌슨의 주관 용어 목록을 바탕으로 한 윌슨의 어휘모듈, 그리고 소수의 의견 약어를 (e.g., imho) 이용한 의견 약어 모듈들 이다. 본 연구의 결과는 여러 가지 방법을 융합하는 것이 의견 검출 성능을 향상시키는데 효과적이 다는 것을 보여주었다.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2004년도 추계학술대회 논문집 Vol.17
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• pp.452-455
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• 2004

In this paper, investigations of the SAMs(self-assembled monolayers) of a thiol-fuctionalized viologen derivatives, $V_8SH$ and $SH_8V_8SH$, where, V is N,N'-dialkylbipyridinium (i.e. a viologen group), have been carried out by elucidate voltammetry date. The redox reactions are highly reversible and can be cycled many times without significant side reaction, which has been known as a nano-gram order mass detector through resonant frequency change self-assembly process of the viologen has been investigated with $QCM({\Delta}F)$. The assembling process of the $V_8SH$ and $SH_8V_8SH$ monolayers can be finished completely in about 1 hour. The measured frequency shift for $V_8SH$ and $SH_8V_8SH$ were about 351 and 172 Hz, respectively. From these values, we calculated that the mass adsorbed $V_8SH$ and $SH_8V_8SH$ were about 375 and 183 ng. We believe that this mass loss is caused by the simultaneous loss of the anions present within the monolayer for charge compensation of the viologen dications and some solvent.

• 한국컴퓨터정보학회논문지
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• 제11권5호
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• pp.75-86
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• 2006

일반적으로 영어를 한국어로 번역할 때, 대부분의 영어 명사 어휘들은 해당 어휘가 사용되는 문맥에 따라 다양한 한국어 명사로 번역될 수 있다. 따라서 영어 원문이 갖는 의미를 손실 없이 번역문으로 전달하기 위해서는 문맥에 맞는 올바른 한국어 대역어를 선택할 수 있어야 한다. 본 논문에서는 동사구패턴, 공기 정보에 기반한 의미벡터, 공기 품사 정보 및 한국어 문맥 통계 정보 등의 다양한 지식을 사용하여 영어 명사 어휘의 대역어를 올바로 선택하는 방안을 제공한다. 동사구 패턴은 사전과 코퍼스를 사용하여 구축되었으며, 의미 벡터는 영어 어휘가 특정 한국어 어휘로 번역될 때 공기하는 정보들의 조건부 확률을 나타낸다. 한국어 문맥 통계 정보는 한국어 코퍼스로부터 추출된 N-그램 정보를 나타내며, 품사 공기 정보는 대역어 선택 모호성을 지니는 영어 어휘와 통계적으로 깊은 관련성을 지니는 품사를 나타낸다. 마지막으로 본 논문에서 제안한 대역어 선택 모호성 해소 방안을 평가하기 위한 실험을 수행하였으며, 실험 결과, 제안하는 방법이 기존의 방법보다 성능이 좋다는 것을 확인할 수 있었다.

• Industrial Engineering and Management Systems
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• 제15권3호
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• pp.224-230
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• 2016

The use of new words, numerous spoken languages, and abbreviations on the Internet is extensive. As such, automatically acquiring words for the purpose of analyzing Internet content is very difficult. In a previous study, we proposed a method for Japanese word segmentation using character N-grams. The previously proposed method is based on a simple state-transition model that is established under the assumption that the input document is described based on four states (denoted as A, B, C, and D) specified beforehand: state A represents words (nouns, verbs, etc.); state B represents statement separators (punctuation marks, conjunctions, etc.); state C represents postpositions (namely, words that follow nouns); and state D represents prepositions (namely, words that precede nouns). According to this state-transition model, based on the states applied to each pseudo-word, we search the document from beginning to end for an accessible pattern. In other words, the process of this transition detects some words during the search. In the present paper, we perform experiments based on the proposed word acquisition algorithm using Japanese and Chinese newspaper articles. These articles were obtained from Japan's Kyoto University and the Chinese People's Daily. The proposed method does not depend on the language structure. If text documents are expressed in Unicode the proposed method can, using the same algorithm, obtain words in Japanese and Chinese, which do not contain spaces between words. Hence, we demonstrate that the proposed method is language independent.

• 정보처리학회논문지B
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• 제11B권2호
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• pp.241-246
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• 2004

본 논문에서는 언어모델의 언어처리 단위로 VCCV(vowel consonant consonant vowel) 단위를 제안하구 기존의 언어처리 단위인 어적 형태소 단위와 비교한다. 어절과 형태소는 어휘수가 많고 높은 복잡도를 가진다. 그러나 VCCV 단위는 작은 사전과 제한된 어휘를 가지므로 복잡도가 적다. 언어모델 구성에 smoothing은 반드시 필요하다. smoothing 기법은 정확한 확률 예측이 불확실한 데이터가 있을 때 더 나은 확률 예측을 위해 사용된다. 본 논문에서는 형태소, 어절, VCCV 단위에 대해 언어모델을 구성하여 복잡도를 계산하였다. 그 결과 VCCV 단위의 복잡도가 형태소나 어절보다 적게 나오는 것을 볼 수 있었다. 복잡도가 적게 나온 VCCV를 기반으로 N-gram을 구성하고 Katz. Witten-Bell, absolute, modified Kneser-Ney smoothing 등의 방법을 이용한 언어 모델에 대해 평가하였다. 그 결과 VCCV 단위의 언어모델에 적합한 smoothing 기법은 modified Kneser-Ney 방법으로 평가되었다.

• Clinical and Experimental Pediatrics
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• 제64권6호
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• pp.293-300
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• 2021

Background: Understanding the epidemiology and prevalence of febrile urinary tract infection (fUTI) in children is important for risk stratification and selecting appropriate urine sample collection candidates to aid in its diagnosis and treatment. Purpose: This study aimed to analyze the epidemiology, etiology, and changes in antibiotic susceptibility patterns of the first fUTI in children. Methods: This retrospective observational cohort study included children younger than 19 years of age who were diagnosed and treated for their first fUTI in 2006-2016. Electronic medical records were analyzed and radiologic images were evaluated. Results: A total of 359 patients (median age, 5.1 months; interquartile range, 3.0-10.5 months) fit the inclusion criteria; of them, 78.0% (n=280) were younger than 12 months old. The male to female ratio was 5.3:1 for patients aged 0-2 months, 2.1:1 for those 3-5 months, and 1.6:1 for those 6-11 months. Beyond 12 months of age, there was a female predominance. Escherichia coli was the leading cause (83.8%), followed by Enterococcus species (6.7%), and Klebsiella pneumoniae (3.6%). Significant yearly increases in the proportions of multidrug-resistant strains (P<0.001) and extended-spectrum beta-lactamase (ESBL) producers (P<0.001) were observed. In patients with vesicoureteral reflux (VUR), the overall recurrence rate was 53.6% (n=15). A significantly higher recurrence rate was observed when the fUTI was caused by an ESBL versus non-ESBL producer (75.0% vs. 30.0%, P=0.03). Conclusion: fUTI was most prevalent in children younger than 12 months of age and showed a female predominance in patients older than 12 months of age. The proportion of ESBL producers causing fUTI is increasing. Carbapenems, rather than noncarbapenems, should be considered for treating fUTI caused by ESBL-producing enteric gram-negative rods to reduce short-term recurrence rates in children with VUR.