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Tube agglutination test is superior than other serological tests for diagnosis of brucellosis in small ruminants  

Rahman, Md. Siddiqur (Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University)
Jahan, Nusrat (Department of English, Ishorgonj Degree College)
Hossain, Mohammad Arif (Department of Paediatrics, Rajshahi Medical College)
Uddin, M.J. (Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University)
Shil, Niraj Kanti (Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University)
Islam, KBM Saiful (Laboratory of Microbial Physiology, Graduate School of Agriculture, Hokkaido University)
Ahasan, Md. Shamim (Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University)
Rahman, A.K.M. Anisur (Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University)
Song, Hee-Jong (Bio-saferty Research Institute and College of Veterinary Medicine, Chonbuk National University)
Publication Information
Korean Journal of Veterinary Service / v.31, no.4, 2008 , pp. 493-496 More about this Journal
Abstract
Brucella spp. are small, non-motile Gram-negative coccobacilli known to cause disease in a number of vertebrate species including humans and brucellosis is one of the world's major zoonoses, alongside bovine tuberculosis and rabies. There are about 33.55 million goats and 1.16 million sheep in Bangladesh. The sheep and goats can significantly play an important role in the economic well being of the resource-poor farmer in Bangladesh. Sexually matured 362 female small ruminants(300 goats and 62 sheep) were examined. Approximately 3-5 ml of blood was collected from the jugular vein of each animal and sera samples were prepared. Samples were then tested for brucellosis by using Rose Bengal test(RBT), plate agglutination test(PAT) and tube agglutination test(TAT). Among 362 small ruminants, irrespective of species(sheep or goat), diagnosed highest in TAT, 2.21%(n=8) and lowest both by RBT & PAT, 1.93%(n=7) and it is concluded that TAT is superior than RBT and PAT.
Keywords
Brucellosis; Small ruminants; Rose Bengal test; Plate and tube agglutination test;
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