• Asian-Australasian Journal of Animal Sciences
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• 제5권1호
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• pp.39-44
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• 1992

Twelve male crossbred calves of 18 months of age were divided into two groups of six each. Animals in both the groups were fed wheat straw ad lib. However, animals in group II had free access to urea molasses mineral block (UMMB) lick. Straw DM intake (kg/100 kg B. Wt. and $g/w^{0.75}\;kg$) was significantly (p < 0.01) higher in group II ($1.95{\pm}0.06$; $75.55{\pm}1.79$) as compared to group I ($1.27{\pm}0.08$; $48.77{\pm}2.43$). Straw DM digestibility coefficient was not significantly different in groups I and II. However, DOMI (kg/100 kg B. Wt.) was significantly (p < 0.01) higher in group II ($0.986{\pm}0.05$) as compared to group I ($0.615{\pm}0.03$). Digestibility coefficient of DM, OM and CP were significantly higher in group II as compared to group I. However, digestibility coefficients of EE, CF and NFE were non-significantly different between the two groups. Animals in group II exhibited significantly (p < 0.01) higher and positive N, Ca and P balances as compared to group I which exhibited negative balances. Total-N, ammonia-N and urea-N in the blood plasma of animals in group II were significantly (p < 0.01) higher as compared to group I.

• Asian-Australasian Journal of Animal Sciences
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• 제12권5호
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• pp.742-746
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• 1999

The potential of Leucaena leucocephala and Prosopis cineraria leaves as nitrogen supplements ($16g/kg\;W^{0.75}/d$) to improve the utilization of a basal diet of rice straw by goats was assessed in a feeding trial. Rice straw supplementation with forage oats (Avena sativa) was fed as a control diet. DMI, $g/kgW^{0.75}$ of rice straw by goats receiving Leucaena was significantly (p<0.05) higher followed by Prosopis and oat fodder. The supplementation of Leucaena improved the digestibility coefficient of DM, OM, CP, NDF and ADF nutrient density. Goats on Prosopis or oat fodder supplemented straw responded similarly in terms of nutrient utilization. The intake ($g/kgW^{0.75}$) of DCP, TDN and nitrogen balance of goats were significantly higher (p<0.05) when Leucaena was fed. It is suggested that the Leucaena may be a useful N-supplement in areas where livestock are fed poor quality crop residues.

• Asian-Australasian Journal of Animal Sciences
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• 제13권5호
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• pp.659-665
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• 2000

A nitrogen (N) balance trial was conducted to examine the effect of N deprivation on subsequent N retention, blood urea nitrogen (BUN) and IGF-I levels and the ratio of IGF binding protein (IGFBP)-3 to IGFBP-l and -2. Pigs in treatment (T) 1 were given diet A (2.39% N) and those in T2 and T3 were given diet B (1.31% N) and excreta were collected (period 1 (P1)). Pigs in T1 continued to receive diet A while diets for T2 and T3 were changed to diets A and C (2.74% N), respectively. The excreta were collected for two more periods (P2 and P3). During P1, pigs in T2 and T3 retained 50% less N (p<0.001) than those in T1. However, pigs provided T2 (p<0.01) and T3 (p<0.05) retained more N than those assigned to T1 during P2. Pigs in T3 tended to retain more (p=0.10) N than those receiving T2 for the same period. The BUN values were lower (p<0.05) for pigs assigned to T2 and T3 than T1 during P1 and P2. Both IGF-I and IGFBP ratios of pigs assigned to T1 were higher (p<0.05) than those given T2 and T3 during P1 but no differences were found during P2 and P3.

• 한국축산식품학회지
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• 제40권4호
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• pp.497-511
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• 2020

This study aimed at comparing the storage stability, fatty acids profile, volatile flavor compounds and eating quality of Hanwoo beef longissimus thoracis (LT) muscles between two marbling morphological groups (fineness and coarseness). The 1⁺ grade Hanwoo LT muscles were measured for marbling fleck morphology at the 13^th thoracic vertebrae location by using computerized image analysis. Results reveal that both marbling groups had similar proximate composition (protein, fat, moisture and collagen), color and technological quality traits (p>0.05). However, the coarse marbling group presented greater C18:2n6 and polyunsaturated fatty acids contents compared to the fine marbling group (p<0.05). Furthermore, the fine marbling group presented lower volatile basic nitrogen (VBN) and 2-thiobarbituric acid reactive substance (TBARS) contents compared to the coarse marbling group after 14 d storage (p<0.05). Also, higher amounts of C18:2n6 oxidation-derived volatile compounds such as hexanal and 3-ethyl-2-methyl-1,3-hexadiene, were found in the coarse marbling group. In term of sensory aspects, the finely-marbled group received higher scores of flavor and taste compared to the coarse marbling group (p<0.05). Overall, the marbling flecks morphology partially showed its effects on the storage stability, fatty acids profile and eating quality of the 1⁺ grade Hanwoo beef LT muscle.

• Asian-Australasian Journal of Animal Sciences
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• 제31권12호
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• pp.1890-1896
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• 2018

Objective: As the climate changes, it influences ruminant's feed intake, nutrient digestibility, rumen methane production and emission. This experiment aimed to evaluate the effect of feeding Sweet grass (Pennisetum purpureum cv. Mahasarakham; SG) as a new source of good quality forage to improve feed utilization efficiency and to mitigate rumen methane production and emission. Methods: Four, growing crossbred of Holstein Friesian heifers, 14 months old, were arranged in a $4{\times}4$ Latin square design to receive four dietary treatments. Treatment 1 (T1) was rice straw (RS) fed on ad libitum with 1.0% body weight (BW) of concentrate (C) supplementation (RS/1.0C). Treatment 2 (T2) and treatment 3 (T3) were SG, fed on ad libitum with 1.0% and 0.5% BW of concentrate supplementation, respectively (SG/1.0C and SG/0.5C, respectively). Treatment 4 (T4) was total Sweet grass fed on ad libitum basis with non-concentrate supplementation (TSG). Results: The results revealed that roughage and total feed intake were increased with SG when compared to RS (p<0.01) while TSG was like RS/1.0C treatment. Digestibility of nutrients, nutrients intake, total volatile fatty acids (VFAs), rumen microorganisms were the highest and CH4 was the lowest in the heifers that received SG/1.0C (p<0.01). Total dry matter (DM) feed intake, digestibility and intake of nutrients, total VFAs, $NH_3-N$, bacterial and fungal population of animals receiving SG/0.5C were higher than those fed on RS/1.0C. Reducing of concentrate supplementation with SG as a roughage source increased $NH_3-N$, acetic acid, and fungal populations, but it decreased propionic acid and protozoal populations (p<0.05). However, ruminal pH and blood urea nitrogen were not affected by the dietary treatments (p>0.05). Conclusion: As the results, SG could be a good forage to improve rumen fermentation, decrease methane production and reduced the level of concentrate supplementation for growing ruminants in the tropics especially under global climate change.

• 한국초지조사료학회지
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• 제19권3호
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• pp.197-202
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• 1999

바랭이(Digitaria sanguinalis L.) 우점 야초지의 건물수량, 사료가치 및 영양소 이용성 등을 구명하여 조사료원으로 활용 가능성을 탐색하고자 숙기별 건물수량, 화학적 성분을 조사하였고, 재래산양 12두를 공시하여 혼파목초와 바랭이 우점 야초를 급여하여 섭취량, 건물소화율 및 질소와 에너지 이용성 등을 평가한 결과는 다음과 같다. 바랭이 우점 야초지의 평균 건물수량은 8,473kg/ha이었으며, 평균 CP, NDF, ADF 및 lignin 함량은 각각 13.0%, 74.1%, 38.6% 및 10.4%이었다. 건물섭취량은 바랭이 우점 야초지가 혼파초지에 비하여 다소 낮은 편이었으나 두 처리간에 유의적인 차이는 없었다. 그러나 건물소화율, 세포내용물의 소화율, NDF 및 ADF 소화율은 바랭이 우점 야초지가 혼파초지에 비하여 낮은 결과를 보였다(P<0.05). 질소 및 에너지의 이용성은 바랭이우점 야초지가 혼파초지에 비하여 낮았다(P<0.05). 이상의 결과로 보아 바랭이 우점 야초지는 혼파초지에 비하여 건물섭취량과 소화율 및 질소와 에너지이용성 등이 다소 낮은 편이지만, 시비를 하지 않고 연간 8,473kg/ha의 건물수량을 얻을 수 있어 부존 조사료원의 하나로 이용 가능성은 크다고 하겠다.

• 한국미생물·생명공학회지
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• 제47권2호
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• pp.195-200
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• 2019

The purpose of this study was to investigate the melanogenesis inhibiting activity of the ethanol extract from Polygonum amphibium L. Firstly, the n-hexane (Hx), chloroform ($CHCl_3$), ethyl acetate (EA), n-butanol (BuOH), and water (Water) fractions were isolated from the P. amphibium L. ethanol extract. The efficacy of melanogenesis was found to significantly decrease via the EA and BuOH fractions when compared to the control in B16F10 cells. EA particularly showed the lowest melanin content in B16F10 cells when compared to all the other extracts. Concentration-dependent inhibition of melanin synthesis was also observed in the EA fraction at concentrations below $50{\mu}g/ml$, which did not exhibit cytotoxicity in B16F10 cells. Notably, the expression of three key proteins (tyrosinase, tyrosinase-related protein-1 (TRP-1), and TRP-2), which are involved in melanogenesis, were significantly decreased via the EA fraction. EA also inhibited body pigmentation in vivo in a zebrafish model. Overall, we demonstrated melanogenesis suppression using the EA fraction from P. amphibium L., which could be a potential candidate for an antimelanogenesis agent.

• Journal of Nutrition and Health
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• 제40권3호
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• pp.211-220
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• 2007

The purpose of this study was to investigate that the effect of dietary fatty acid composition on pro- and macro-glycogen utilization and resynthesis. The analyses were further extended for different muscle fibers (type I, type II, & type IIb) as well as tissues (i.e., liver & heart). Total one hundred sixty Sprague-Dawley rats were used, and rats were randomly allocated into four experimental groups: animals fed standard chow diet (n=40), animals fed saturated fatty acid diet (n=40), animals fed monounsaturated fatty acid (n=40), and animals fed polyunsaturated fatty acid (n=40). Animals in each groups were further divided into five subgroups: sacrificed at REST (n=8), sacrificed at immediately after 3 hr swim exercise (P-0HR, n=8), sacrificed at one hour after 3 hr swim exercise (P-1HR, n=8), sacrificed at four hour after 3 hr swim exercise (P-4HR, n=8), and sacrificed at twenty-four hour after 3 hr swim exercise (P-24HR, n=8). Soleus (type I), red gastrocnemius (type IIa), white gastrocnemius (type IIb), liver, and heart were dissected out at appropriated time point from all animals, and were used for analyses of pro- & macro-glycogen concentrations. After 8 weeks of dietary interventions, there was no significant difference in body mass in any of dietary conditions (p>.05). After 3 hr swim exercise, blood lactate level was higher compared to resting conditions in all groups, but it was returned to resting value after 1 hr rest (p<.05). Free fatty acid concentration was higher in all high fat fed groups(regardless of fatty acid composition) than CHOW consumed group. At rest, pro- & macro-glycogen concentration was not different from any of experimental groups (p>.05). Regardless of forms of glycogen, the highest level was observed in liver (p<.01), and most cases of supercompensation after 3hr exercise observed in this study were occurred in CHOW fed tissues. Except heart muscle, all tissues used in this study showed that pro- and macro-glycogen concentration was significantly decreased after 3 hr exercise. Based on these results, two conclusions were made: first, there is no different level of glycogen content in various tissues regardless of types of fatty acids consumed and second, the highest mobilization rate would be demonstrated from CHOW fed animals compare to animals that consumed any kinds of fatty acid diet if prolonged exercise is applied.

• Asian-Australasian Journal of Animal Sciences
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• 제13권12호
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• pp.1659-1666
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• 2000

Two experiments were conducted to determine the effects of Leucaena leucocephala (leucaena) and Gliricidia sepium (gliricidia) supplementation on intake, digestion, outflow rates, microbial protein yield and live-weight changes in sheep fed with ammoniated rice straw (ARS). In experiment 1, three rumen cannulated Japanese Corriedale wether (mean body weight of 35.6 kg) in $3{\times}3$ Latin Square Design were used. Animals were fed ad libitum ARS alone, or supplemented with 200 g of either leucaena or gliricidia. In experiment 2, twenty-four growing native Philippine sheep with average body weight of $13.5{\pm}0.25kg$ were used in a completely randomized design (CRD) and offered similar diets to those of experiment 1. Supplementation increased total dry matter intake and nutrient digestibility except for fiber (p<0.05) without affecting ARS consumption. Nitrogen balance revealed that absorbed and retained N was significantly higher in leucaena and gliricidia. The significant improvement in N utilization and more digestible OM intake brought about by the inclusion of leucaena and gliricidia to ARS resulted in increased (p<0.05) microbial N yield. Efficiency of microbial N supply in supplemented group was not significantly different, but higher (p<0.05) than the 24.92 g N/kg DOMR for ARS group. Liquid outflow rate was 7.8 and 6.8 %/h, while the solid phase of rumen digesta was 4.4 and 3.8 %/h for the leucaena and gliricidia group respectively, which were significantly higher than 5.30 and 2.50 %/h in the control diet. The increase in total DMI resulted to higher (p<0.01) growth performance and efficient feed utilization. Average daily gain (ADG) was 19.3, 34.6 and 33.9 g/d for the ARS, leucaena and gliricidia respectively. It is therefore concluded that addition of leucaena and gliricidia to ARS in could increase nutrient intake and digestibility, subsequently improving N utilization and livestock performance.

• 한국미생물·생명공학회지
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• 제41권1호
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• pp.52-59
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• 2013

The nutritional requirements for the maximum production of lipopeptides by Bacillus subtilis N7 (B. subtilis N7) were investigated and optimized using response surface methodology (RSM) under shake flask fermentation. A one-factor-at-a-time experimental setup was used to screen carbon and nitrogen sources. A Plackett-Burman design (PBD) was employed to screen the most critical variables for lipopeptides production amongst ten nutritional elements. The central composite experimental design (CCD) was finally adopted to elucidate the composition of the fermentation medium. Statistical analyses (analysis of variance, ANOVA) of the results showed that KCl, $MnSO_4$ and $FeSO_4{\cdot}6H_2O$ were important components and that their interactions were strong. Lipopeptide production was predicted to reach 709.87 mg/L after a 60 h incubation using an optimum fermentation medium composed of glucose 7.5 g/L, peanut oil 1.25 g/L, $MgSO_4$ 0.37 g/L, $KH_2PO_4$ 0.75 g/L, monosodium glutamate 6.75 g/L, yeast extract and $NH_4Cl$ (5:3 w/w) 10 g/L, KCl 0.16 g/L, $FeSO_4{\cdot}6H_2O$ 0.24 mg/L, $MnSO_4$ 0.76 mg/L, and an initial pH of 7.0. Lipopeptide production ($706.57{\pm}3.70$ mg/L) in the optimized medium confirmed the validity of the predicted model.