• Title/Summary/Keyword: Myzus persicae (Sulzer)

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연초포장에서 발생하는 복숭아혹진딧물(Myzus persicae Sulz.)의 Esterase 분류

  • 채순용;김상석;정성은;장영덕
    • Journal of the Korean Society of Tobacco Science
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    • v.21 no.1
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    • pp.49-56
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    • 1999
  • Classification of esterase isozymes of the apterous green peach aphids (Myzus persicae Sulzer) collected in tobacco fields were investigated by the native polyacrylamide gel electrophoresis (PAGE). A total of twelve esterase bands were identified in adult apterous aphid, and the difference of enzyme band activity in the clones was observed at the first and second bands group. Esterases of green peach aphids reacted with specific substrate were more stained $\alpha$-naphthyl acetate than $\alpha$-naphthyl propionate, and $\alpha$-naphthyl acetate more than $\beta$-naphthyl acetate. Twelve esterases on the basis of inhibition by the three types of inhibitors (organophosphates: 2.5$\times$10$^{-3}$ M paraoxon, 4$\times$10$^{-3}$ M DFP; eserine sulfate : 2$\times$10$^{-3}$ M eserin; sulfhydryl reagents: 2$\times$10$^{-3}$ M p-HMB) were classified into three class, namely, cholinesterase (ChE) I, II, carboxylesterase (CE) and arylesterase (ArE), and these classes contained 3, 4, 3 and 2 isozymes, respectively.

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Insecticidal Activity of Coptis chinensis Extract Against Myzus persicae (Sulzer)

  • Jung, Ji Young;Lee, Hyung Chul;Yang, Jae-Kyung
    • Journal of the Korean Wood Science and Technology
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    • v.43 no.2
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    • pp.274-285
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    • 2015
  • In view of the environmental and health hazards posed by synthetic insecticides, the use of plant products as botanical insecticides has gained increasing in recent years. In this study, we reported the insecticidal activity of extracts isolated from Coptis chinensis. On crude extraction, among the various solvent types tested (water, 1% (w/v) of sodium hydroxide, 70% ethanol), the 70% ethanol extract showed the best insecticidal activity (36.5%). Three different fractions (n-hexane, chloroform and ethyl acetate) were obtained from crude extract (70% ethanol) of the chloroform fraction and found to have noteworthy insecticidal activity (62.9%) by filter paper contact bioassay. Their chemical structures were identified as 2-methoxy-4-vinylphenol and aniline by head space-GC-MS analysis. Both compounds displayed a dose-dependent insecticidal activity of Myzus persicae (Sulzer). Insecticidal activity at the lowest concentration tested (500 ppm) approached 85.4% in the aniline compared with 79.9% in the 2-methoxy-4-vinylphenol. The insecticidal activity was greater for the aniline than 2-methoxy-4-vinylphenol. It is believed that the insecticidal activity is due mainly to the presence of aniline.

Absence of DNA Polymorphisms in Myzus persicae (Homoptera: Aphididae) in Relation to their Host Plants (기주식물 종류에 따른 복숭아혹진딧물(Myzus persicae)의 DNA Polymorphism 비교)

  • H. J. Kim;K. S. Boo;K. H. Cho
    • Korean journal of applied entomology
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    • v.35 no.3
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    • pp.209-215
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    • 1996
  • DNA polymorphisms were analyzed for 8 clones of the green peach aphid, Myzus persicae Sulzer, by random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR). The insect has different host preferences and was even classified into two different species, M. persicae Sulzer and Myzus nicotinae Blackman by their morphological characters, but this point is still in arguement. To identify the differences between two types of the green peach aphid by RAPD-PCR, the template DNA was extracted from 4 clones each of tobacco-feeding and non-tobacco-feeding forms and one hundred primers of 10-nucleotideslong were tested in PCR. The amplified DNAs were analyzed by agarose gel electrophoresis. Eighty-three primers gave amplified DNA fragments with 1 to 22 in number and 500 to 20,000 base pairs in length, but no amplification was observed in the other 17 primers. The average number of fragment per each amplification was about 13. In the case of 82 out of 83 random primers, band patterns of amplified DNA were identical among 8 clones, even though some differences were noticed in the intensity of specific bands. Polymorphism was detected by only one primer within the tobacco-feeding forms, but not between the two host types. The results did not detect any relationship between RAPD polymorphism and their host preference.

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Joint Toxic Action of Insecticide Mixtures to the Cupermethrin-and Pirimicarb-Selected Strains of Green Peach Aphid(Myzus pericae Sulzer) (Cypermethrin과 Pirimicarb 저항성계통 복숭아혹잔딧물에 대한 살충제문의 연합독작용)

  • 안용준;김길하;최승윤
    • Korean journal of applied entomology
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    • v.28 no.1
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    • pp.32-36
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    • 1989
  • The joint toxic action of mixtures of cypermethrin or pirimicarb with one of other insecticides (acephate, cypermethrin, demeton-S-methyl and pirimicarb) on the cypermethrin or picimicarb-selected green peach aphid (Myzus persicae Sulzer)was investigated. The responses depended on the choice and ratios of insecticide combination. In the cypermethrin-selected strain bioas-say, mixtures of test insecticides showed no synergistic effect. On the other hand, the maxi-mum synergistic effects for the pirimicarb-selected strain were obtained at the 8 : 2 ratio of pirimicarb and demeton-S-methyl exhibited antagonistic effect.

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Seasonal fluctuation of susceptibility of the green peach aphid to insecticides in Chinese cabbage field (포장에서 복숭아혹진딧물(Myzus persicae Sulzer)의 계절적 변동에 따른 약제 감수성)

  • ;Naoki Motoyama
    • Korean journal of applied entomology
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    • v.32 no.2
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    • pp.218-221
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    • 1993
  • Sensitivities of insecticides and their seasonal variation were investigated against Myzus persicae. Some aphicides such as malathion were not effective on Myzus persicae in Chinese cabbage field. Difference of seasonal sensitivity was from I time to 16 times.

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Genetic Relationahips of the Two Morphorogical Types of Myzus persicae(Homoptera:Aphididae) Collected from Tobacco Plants Based on Random Amplified Polymorphic DNA(RAPD) (연초에서 발생하는 복숭아혹진딧물(Myzus persicae)형태형 2종의 Random Amplified Polymorphic DNA(RAPD)을 이용한 유전적 유연관계 분석)

  • 채순용;이기원;김상석;장영덕
    • Korean journal of applied entomology
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    • v.37 no.1
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    • pp.31-37
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    • 1998
  • Random amplified polymorphic DNA (RAPD) markers were used to analyze genetic similarity among 8 clones of apierous green peach aphid, two types (M. persicae Sulzer and M. nicotianae lack man) classified by their mo~hologi~cahla raters and host preference (Blackman, 1987), collected from tobacco plants. The genetic variation among these clones was evaluated by polymerase chain reaction amplification with 20 random primers. The higher GC contents of primers, the better in amplification efficiency of PCR reaction in general. The genetic similarities among eight aphid clones were analyzed from UPGMA (unweighted pair group average method) cluster analysis based on simple matching coefficient. The range of genetic similarity coefficients was 0.414 to 0.808. The most close relationship among the clones was similarity coefficient of 0.808 between the PG2 and the PG3 clone. The eight aphid clones analyzed were clustered into three groups by the genetic similarity coefficient. The first group, PG1, PG2, PG3 clone including in M. persicae type by their morphological characters and RED clone in M. nicotianae type was clustered at the genetic similarity coefficient of 0.643. The second group, GR1, GR2, BRN in M. nicotianae type was at the 0.636;and the third group was DBR clone in M. persicae type. The results did not indicate any correlation between m&-phological types (M. persicae and M. nicotianae) and RAPD polymorphism. We could not detect any obvious genetic relationships of the two morphological types of the green peach aphid collected from tobacco plants.

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Studies of the Insecticide Resistance in the Green Peach Aphid, Myzus persicae Sulzer (V). Development of Cypermethrin and Pirimicarb Resistance, and Cross Resistance (복숭아혹진딧물의 살충제 저항성에 관한 연구 (V). Cypermethrin과 Pirimicarb에 의한 저항성 발달과 교류저항성)

  • 최승윤;김길하;안용준
    • Korean journal of applied entomology
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    • v.28 no.1
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    • pp.23-27
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    • 1989
  • The green peach aphid(Myzus pericae Sulzer) was selected over 20 generations with cypermethrin and pirmicarb, respectively. The resulting resistant strains were tested to inverting-ate the development of insecticide resistance and cross-resistance to some insecticides in the laboratory. The development of insecticide resistance against green peach aphid at the 20th selected generation was greatly varied with the insecticides: 20.5 fold for cypermethrin and 3.2 fold for pirimicarb compared with the parent strain. The cypermethrin selected strain exhibited cross resistance to acephate and pirimicarb, and pirimicarb selected strain to acephate and cypermethrin, respectively. Demeton-S-methyl, however, has not been shown cross-resistance by the selected strains.

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Occurrence and Ecological Characteristics of Colour Morphs of the Green Peach Aphid, Myzus Persicae (Sulzer) (Homoptera : Aphididae) in Tobacco Field (담배포장에서의 복숭아혹진딧물 체색형별 발생과 생태적 특성)

  • 손준수;송유한
    • Journal of the Korean Society of Tobacco Science
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    • v.16 no.1
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    • pp.76-83
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    • 1994
  • The dominant colour - types of fundatrices of green peach aphid, Myzsts persicae 5. were yellow - green, red and green. Yellow type was the minority among nymphs produced by field collected alatae. Rate of producing dead nymphs was over 34.0% in red and yellow types apterae grown from tobacco plants in early summer. Brown and green were the dominant colour - type in apterae throughout tobacco growing season. Brown type on tobacco, and yellow type on hot pepper and tomato grew better and produced more nymphs than other colour - types.

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Development and Predation of a Aphidophagous Gall Midge, Aphidoletes aphidimyza (Roundani) (Diptera: Cecidomyiidae) on Myzus persicae Sulzer (진딧물류를 포식하는 혹파리, Aphidoletes aphidimyza(Roundani)(Diptera: Cecidomyiidae)의 복숭아혹진딧물 포식과 발육)

  • 최만영;이건휘;백채훈;김두호
    • Korean journal of applied entomology
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    • v.40 no.1
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    • pp.44-49
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    • 2001
  • Development and predation of an aphidophagous gall midge, Aphidoletes aphydimyza (Rondani) , on the green peach aphid, Myzus persicae Sulzer, were studied under laboratory and greenhouse conditions. The developmental threshold temperature of the gall midge larvae was estimated to be $13^{\circ}C$. Larval and total developmental periods were 5.3 and 29 days at 25 $\pm$$2^{\circ}C$. The gall midge attacked more aphids as increasing density of peach aphids, and consumed 4 out of 10 peach aphids per day in a petri dish (9 cm in diameter) at maximum. In green house, the gall midge could control the density of the green peach aphids efficiently with a delayed density dependent-like pattern.

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Karyotype and Insecticide Resistance of the Green Peach Aphids (Myzus persicae Sulzer) collected from Tobacco fields (연초포장에서 발생하는 복숭아혹진딧물의 핵형과 살충제 저항성)

  • 채순용;김영호;김상석;장영덕
    • Journal of the Korean Society of Tobacco Science
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    • v.19 no.2
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    • pp.107-116
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    • 1997
  • Karyotype of apterous green peach aphid (Myzus persicae SuIBer) populations occurring in tobacco fields and their resistance to insecticides were investigated. All of the 16 clones collected from tobacco fields had same chromosome number of 2n=12. Among them, 5 clones had the normal karyotype, regardless of color morph, but chromosomal translnations between #1 and #3 autosomal chromosomes were noted in the other 11 clones. All of the aphid clones were highly resistant tolambda cyhalothrin, a pyrethroid pesticide, having over 20 times higher concentrations of LD90 than recommended concentration (10ppm) for the aphid. However, their resistance to acephate, demeton 5-methyl and pirimicarh varied depending on the clones, among which green-colored clones were generally more resistant to the pesticides than red-colored ones. No association was found between the insecticide resistance and the karyotype of the aphid in this experiment.

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