• Analytical Science and Technology
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• v.13 no.5
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• pp.630-635
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• 2000

The concentration of neurotransmitters in rat brain was determined by HPLC-ECD (electrochemical detection) method and the effects of methanol extracts of some crude drugs, such as Polygala Radix, Myristicae Semen, Zizyphi Semen, Acori graminei Rhizoma, Visci Herba, Liriopsis Tuber, Myrrha on the concentration of neurotransmitters in the ethanol-treated rat brain were investigated. By the administration of ethanol, dopamine (DA), 3, 4-dihydroxyphenyl acetic acid (DOPAC) and serotonin (5-HT) levels in frontal cortex and 5-HT level in hippocampus were significantly increased compared with the neurotransmitter levels in the brain of saline-treated rats. The ${\gamma}$-aminobutyric acid (GABA) level in frontal cortex was decreased by the same treatment. There was a tendency that the DA level in frontal cortex and striatum of ethanol-treated rats were increased by the administration of crude drug extracts. Especially, Myrrha and Visci Herba significantly increased the DA level of frontal cortex in ethanol-treated rats, while they significantly decreased the 5-HT level in the same region of the brain. GABA level in striatum of ethanol-treated rats was significantly decreased by Myristicae Semen, Visci Herba and Myrrha. These results suggest that the tested crude drug extracts have selective interaction with neurotransmitters in specified region of central nervous system.

• Journal of Periodontal and Implant Science
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• v.21 no.1
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• pp.173.1-173.1
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• 1991

• Biomedical Science Letters
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• v.7 no.3
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• pp.145-149
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• 2001

The present study was conducted to determine whether local production of neutrophil chemoattractant cytokines preceded the influx of neutrophils 1311owing dermal scald burn injury. In order to inhibit the influx of neutrophils, we induced scald burn injury on dorsal skin of rats (TBSA 15%) and spread Rhubarb (R. undulatum L.) and complex (Rhubarb+Olibanum+Myrrha) on scald skin in rats. 1. Levels of dermal KC, the indicator of skin iuflammation, caused by burn gradually increased 5 hours, 24 hours (p<0.001) and 72 hours (p<0.001) after the burn occurred. After 24 hours it occurs, KC in skin was decreased by both skin spread of Rhubarb (p<0.05) and complex (p<0.05) compared to scald bum group. After 72 hours, KC by skin sp.end of Rhubarb and complex was significantly decreased (p<0.01), and the inhibition effect of KC production by complex is higher than by Rhubarb. 2. KC content in serum was increased (p<0.01) at 24 hours after burn injury and comes down back to the normal level after 72 hours. At 24 hours postbunt both Rhubarb (p<0.05) and complex (p<0.01) skin spread decreased KC in serum. The inhibition effect of KC production by complex skin spread group is higher than by Rhubarb skin spread group. The data suggest that the KC production effects by Rhubarb skin spread group and complex skin spread group are high. Especially the endurance of the effect by complex skin spread group was longer than by Rhubarb skin spread group. The inhibition effect of KC production by complex skin spread group increases more than by Rhubarb skin spread group as time passes.

• The Journal of Korean Obstetrics and Gynecology
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• v.19 no.1
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• pp.97-110
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• 2006

Purpose : To address the ability of Myrrha (MY) to induce cell death, we investigated the effect of MY on apoptosis. In human cervical carcinoma HeLa cells, apoptosis occurred following MY exposure in a dose-dependent manner. Methods : We have tested several kinds of anti-oxidants to investigate the MY-induced apoptotic mechanism. Among the anti-oxidants, N-acetyl cysteine(NAC) or reduced glutathione (GSH) protects MY-induced apoptosis. NAC is an aminothiol and synthetic precursor of intracellular cysteine and GSH. To confirm the role of GSH in MY-induced apoptosis, methionine and cystathionine-glutathione extrusion inhibitors were treated in the presence of MY. Results : NAC, GSH, methionine or cystathionine led to protective effect against MY-induced apoptosis in HeLa cells. The GSH and GSH-associated reagents regulate MY-induced cytochrome c release and the resultant caspase-3 activation. Furthermore, the two specific inhibitors of carrier-mediated GSH extrusion, methionine and cystathionine demonstrate GSH extrusion occurs via a specific mechanism. While decreasing GSH extrusion and protecting against MY-induced apoptosis, methionine and cystathionine failed to exert anti-apoptotic activity in cells previously deprived of GSH. Conclusion : the target of the protection is indeed GSH extrusion. This shows that the protective effect is achieved by forcing GSH to stay within the cells during apoptogenic treatment. All this evidence indicates the extrusion of GSH precedes andis responsible for the apoptosis, probably by altering the intracellular redox state, thus giving a rationale for the development of redox-dependent apoptosis in MY-treated human cervical carcinoma HeLa cells.

• The Korea Journal of Herbology
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• v.25 no.2
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• pp.21-39
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• 2010

Objectives & Method : We investigated toxicity, poisoning symptoms, poisoning treatment and prevention against poisoning of herbal medicines used to activate blood flow and remove blood stasis(活血祛瘀藥) in order to use herbal medicines accurately. Result : Cnidii Rhizoma(川芎), Olibanum(乳香), Myrrha(沒藥), Corydalis Tuber(玄胡索), Zedoariae Rhizoma(莪朮), Salviae Miltiorrhizae Radix(丹參), Polygoni Cuspidati Radix(虎杖根), Leonuri Herba(益母草), Persicae Semen(桃仁), Carthami Flos(紅花), Manitis Squama(穿山甲), Eupolyphaga(蟅蟲), Hirudo(水蛭), Vaccariae Semen(王不留行), Sappan Lignum(蘇木), Lacca Sinica Exsiccata(乾漆), Draconis Resina(血竭) and Leonuri Semen(茺蔚子) may give rise to some side effects or toxic symptoms in herbal medicines used to activate blood flow and remove blood stasis(活血祛瘀藥). The representative methods of poisoning treatment in western medicines are washing out the stomach, promotion of vomiting, causing diarrhea, supplies of grape sugar and symptomatic treatment, etc. The representative methods of poisoning treatment in oriental medicine take advantage of herbs. And Oriental medical doctor should meet symptoms as patients call for attention. In order to prevent against poisoning of herbal medicines used to activate blood flow and remove blood stasis (活血祛瘀藥), the patients should keep usage, dosage and notes and oriental medical doctors should do processing drugs. Conclusion : We should pay attention to clinical using of Cnidii Rhizoma(川芎), Olibanum(乳香), Myrrha(沒藥), Corydalis Tuber(玄胡索), Zedoariae Rhizoma(莪朮), Salviae Miltiorrhizae Radix(丹參), Polygoni Cuspidati Radix(虎杖根), Leonuri Herba(益母草), Persicae Semen(桃仁), Carthami Flos(紅花), Manitis Squama(穿山甲), Eupolyphaga(蟅蟲), Hirudo(水蛭), Vaccariae Semen(王不留行), Sappan Lignum(蘇木), Lacca Sinica Exsiccata(乾漆), Draconis Resina(血竭) and Leonuri Semen(茺蔚子) in herbal medicines used to activate blood flow and remove blood stasis(活血祛瘀藥).

• Journal of Oriental Physiology
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• v.14 no.2 s.20
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• pp.127-137
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• 1999

The current experiment was carried out to investigate the analgesic effects of several herbal drugs in acetic acid - induced pain model. In a single drug group : after administration of herbal drugs(1g/kg or 3g/kg) orally for 30 minutes, 1% acetic acid $(250{\mu}l)$ was administered into abdominal cavity of mouse. And then the number of times of writhing response was measured for 30 minutes. In a combination drug group : after administration of herbal drugs (1g/kg and it's compound 2g/kg) orally for 30 minutes, 1% acetic acid $(250{\mu}l)$ was administered into abdominal cavity of mouse. And then the number of times of writhing response was measured for 30 minutes. The results were summarized as follows; 1. Water extracts of Akebiae caulis(木通) and Stephaniae tetrandrae radix(防己) decreased significantly the number of writhing response. 2. Methanol extracts of Achyranthis bidentatae radix(牛膝), Carthami flos(紅花), Akebiae caaulis(木通), Stephaniae tetrandrae radix(防己), Myrrha(沒藥), Corydalidis tuber(玄胡索) and Persicae semen(桃仁) decreased significantly the number of writhing response. 3. Water extracts of Achyranthis bidentatae radix(牛膝) plus Akebiae caulis(木通), Achyranthis bidentatae radix(牛膝) plus Stephaniae tetrandrae radix(防己) and Achyranthis bidentatae radix(牛膝) plus Ledebouriellae radix(防風) decreased the number of writhing response significantly. 4. Methanol extracts of Achyranthis bidentatae radix(牛膝) plus Myrrha(沒藥), Achyranthis bidentatae radix(牛膝) plus Stephaniae tetrandrae radlx(防己) and Achyranthis bidentatae radix(牛膝) plus Ledebouriellae radix(防風) decreased the number of writhing response significantly.

• Journal of Periodontal and Implant Science
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• v.29 no.4
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• pp.751-765
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• 1999

Several growth factors and polypeptidesare not commonly yet used for regenerators of bone tissue or alveolar bone because of the insufficiency of studies on their side effects, genetic engineering for mass production and stability for clinical application. Recently, many natural medicines, which have advantage of less side effects and possibility of long-term use, have been studied for their capacity and effects of anti-bacterial, anti-inflammatory and regenerative potential of periodontal tissues. Olibanum, Myrrha, Phlomis Radix, and Cimicifugae Rhizoma have been traditionally used as a drug for treatment of bone disease in oriental medicine. The objective of this study was to examine the ability of alkaline phosphatase(ALP) synthesis of rat calvarial osteoblast(MC3T3-E1) when several natural medicines were supplemented. MC3T3-E1 cells were cultured with ${\alpha}$-MEM(negative control), dexamethasone(positive control), and each natural medicines for 3 and 5 days. And then ALP synthesis was measured by spectrophotometer for enzyme activity and by naphthol AS-BI staining for morphometry. All of the natural medicines induced higher activity of ALP synthesis than the negative controls. Especially Olibanumind uced the higher activity than the positive controls (p<0.05). In the aspects of culturing time, except Cimicifugae Rhizoma, the natural medicines induced higher activity of ALP synthesis at 5 days than at 3 days (p<0.05). In morphometry, all of the natural medicines showed statistical significance compared to the negative control (p<0.05). Myrrha a n d Phlomis Radix showed larger positively stained area at 5days than at 3 days, whereas the others did not showed the difference between at 5 and at 3 days(p<0.05). These results indicate that several natural medicines have an inducing ability of ALP synthesis in MC3T3-E1 cells.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.13 no.2
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• pp.51-75
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• 2000

The result were as follows: 1. Apply the drug to the affected part can used every time of ulcer. 2. Apply the drug to the affected part is used cold drug on yang-syndrome, hot drug on yin-syndrome, regulate drug on ban yin and ban yang-syndrome. 3. Apply the drug to the affected part is used alcohol, water, allii radix(인), zingiberis rhizoma recens(姜), juice of chrysanthemi flos(菊花) and so forth. 4. Apply the drug to the affected part can't used hot drug on yang-syndrome, cold drug on yin-syndrome. 5. Apply the drug to the affected part is used to be very busy of YouYuiKimHoangSan(如意金黃散). 6. Apply the drug to the affected part is used to be very busy on the angelicae adhuricae radix(白芷) of disperse the edema and drain the pus, and on the arisaematis rhizoma(南星), rhei radix et rhizoma(大黃), olibanum(乳香), phellodendri cortex(黃柏), calomelas(輕粉), glycyrrhizae radix(甘草), angelicae gigantis radix(當歸), myrrha(沒藥) of clearing away heat, activating blood circulation and relieve pain. 7. Apply the drug to the affected part is clearing away heat, activating blood circulation 8. Apply the drug to the affected part is cold and hot. 9. Apply the drug to the affected part is pungent, bitter and sweet. 10. Apply the drug to the affected part is non-toxic. 11. Apply the drug to the affected part is used to be very busy on the chanel of liver, heart, spleen, lung, stomach.

• Korean Journal of Pharmacognosy
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• v.33 no.3 s.130
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• pp.211-218
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• 2002

The methanol extracts of 340 kinds of Korean medicinal herbs were examined in vitro for the inhibitory effect on the acetylcholinesterase (AChE, E.C. 3.1.1.7) from electric eel using acetylthiocholine as a substrate. Among tested, the extracts of Coptidis Rhizoma, Phellodendri Cortex, Evodiae Fructus, Myrrha, Arecae Semen and Piperis nigri Fructus were found to exhibit a significant inhibition upon the acetylcholinesterase in a dose dependent manner, respectively.

• Nuclear Engineering and Technology
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• v.46 no.5
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• pp.595-604
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• 2014

SILER (Seismic-Initiated event risk mitigation in LEad-cooled Reactors) is a Collaborative Project, partially funded by the European Commission in the $7^{th}$ Framework Programme, aimed at studying the risk associated to seismic-initiated events in Generation IV Heavy Liquid Metal reactors, and developing adequate protection measures. The project started in October 2011, and will run for a duration of three years. The attention of SILER is focused on the evaluation of the effects of earthquakes, with particular regards to beyond-design seismic events, and to the identification of mitigation strategies, acting both on structures and components design. Special efforts are devoted to the development of seismic isolation devices and related interface components. Two reference designs, at the state of development available at the beginning of the project and coming from the $6^{th}$ Framework Programme, have been considered: ELSY (European Lead Fast Reactor) for the Lead Fast Reactors (LFR), and MYRRHA (Multi-purpose hYbrid Research Reactor for High-tech Applications) for the Accelerator-Driven Systems (ADS). This paper describes the main activities and results obtained so far, paying particular attention to the development of seismic isolators, and the interface components which must be installed between the isolated reactor building and the non-isolated parts of the plant, such as the pipe expansion joints and the joint-cover of the seismic gap.