• 제목/요약/키워드: Myricetin-3-O-${\alpha}$-L-rhamnoside

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Nematicidal Compounds from the Leaves of Schinus terebinthifolius Against Root-knot Nematode, Meloidogyne incognita Infecting Tomato

  • Abdel Bar, Fatma M.;Ibrahim, Dina S.;Gedara, Sahar R.;Abdel-Raziq, Mohammed S.;Zaghloul, Ahmed M.
    • Natural Product Sciences
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    • 제24권4호
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    • pp.272-283
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    • 2018
  • The root-knot nematode, Meloidogyne incognita caused a serious damage to many plants. The phenolic components of the leaves of Schinus terebinthifolius were investigated as potential nematicidal agents for M. incognita. Nine compounds were isolated and characterized as viz., 1,2,3,4,6-pentagalloyl glucose (1), kaempferol-3-O-${\alpha}$-L-rhamnoside (Afzelin) (2), quercetin-3-O-${\alpha}$-L-rhamnoside (Quercetrin) (3), myricetin (4), myricetin-3-O-${\alpha}$-L-rhamnoside (Myricetrin) (5), methylgallate (6), protocatechuic acid (7), quercetin (8), and gallic acid (9) using nuclear magnetic resonance (NMR) spectroscopy. Compound 1 showed pronounced nematicidal activity compared to Oxamyl as a positive control. It showed the lowest eggs-hatchability (34%) and the highest mortality in nematode population (21% after 72 hours of treatment) at a concentration of $200{\mu}g/mL$. It exhibited the best suppressed total nematode population, root galling and number of eggmasses in infected tomato plants. The total carbohydrates and proteins were also significantly induced by 1 with reduction in total phenolics and increase in defense-related proteins. Thus, compound 1 could be a promising, more safe and effective natural nematicidal agent for the control of root-knot nematodes.

박태기나무의 잎으로부터 피부멜라닌 색소생성 억제성분의 분리 (The Isolation of the Inhibitory Constitutents on Melanin Polymer Formation from the Leaves of Cercis chinensis)

  • 김소영;김진준;장태수;정시련;이승호
    • 생약학회지
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    • 제30권4호
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    • pp.397-403
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    • 1999
  • Tyrosinase plays an important role in the process of melanin polymer biosynthesis. Therefore, the enzyme inhibitors have been of great concern as cosmetics to have skin-whitening effects on the local hyperpigmentation. During the search for new inhibitory compounds on melanin polymer biosynthesis from natural sources, MeOH extracts of 589 higher plants were tested for the inhibitory effect on tyrosinase activity by the muschroom tyrosinase assay in vitro. Among plants tested, the leaves of Cercis chinensis exhibited potent inhibitory effect on mushroom tyrosinase activity. Subsequently seven active compounds were isolated from the ethyl acetate soluble part of acetone extract of the leaves of C. chinensis by the activity guided fractionation monitoring the inhibitory effect on tyrosinase activity. Their chemical structures were identified as $kaempferol-3-0-{\alpha}-L-rhamnoside$, quercitrin, $myricetin-3-0-{\alpha}-L-rhamnoside$, myricetin-3-0-(2'-O-galloyl)- ${\alpha}$ -L-rhamopyranoside (desmanthin), (-)-epicatechin-3-0-gallate, (-)-epigallocatechin-3-0-gallate, and methyl gallate on the basis of the speculation of spectral data and chemical reaction. Among the flavonol rhamnosides, myricetin-3-0-(2'-O-galloyl)- -L-rhamnoside(desmanthin) showed most potent inhibitory effect on tyrosinase activity and the structure of B-ring in flavonol moiety was related to the activity. (-)-Epigallocatechin-3-O-gallate having pyrogallol group in flavan-3-ol moiety exhibited more potent inhibitory effect than (-)-epicatechin-3-0-gallate having catechol group in flavan-3-ol moiety on mushroom tyrosinase activity.

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Flavonoids from the Leaves of Betula platyphylla var. latifolia

  • Lee, Min-Won
    • 생약학회지
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    • 제25권3호
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    • pp.199-203
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    • 1994
  • Chemical examination of the leaves of Betula platyphylla var. latifolia has led to the isolation and characterization of five flavonoid glycosides including two C-glucosyl flavonoids. The structures of these compounds were elucidated as myricetin $3-O-{\alpha}-_L-rhamnoside$ (myricitrin), $quercetin-3-O-{\beta}-_D-glucopyranoside$ (isoquercitrin), $quercetin-3-O-{\beta}-_D-glucopyranoside$ (hyperoside), $nalingenin-6-C-{\beta}-_D-glucopyranoside$ (hemiphloin) and $aromadendrin-6-C-{\beta}-_D-glucopyranosidre(6-C-glucosyldihydrokaempferol)$ on the basis of physico-chemical and spectroscopic evidences.

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고욤나무 잎으로부터 활성유도 분획법에 의한 α-Glucosidase 저해물질 분리 및 확인 (Bio-assay Guided Isolation and Identification of α-Glucosidase Inhibitors from the Leaves of Diospyros lotus)

  • 김상준;김지애;김다혜;곽설화;유강열;장선일;김선영;정승일
    • 생약학회지
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    • 제46권2호
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    • pp.105-108
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    • 2015
  • To establish the anti-diabetic(α-glucosidase inhibitory) activity of D. lotus leaf extract, isolate and identify the constituents responsible for the activity. The methanolic extract of leaves was partitioned between water, n-butanol and ethyl acetate. Bio-assay guided fractionation, based on inhibition of ;${\alpha}$-glucosidase, allowed isolation and identification of the active components. Liquid chromatography/mass spectrometry(LC/MS), 1 H-NMR and 13 C-NMR spectra analyses demonstrated that the active compound was myricetin-3-O-;${\alpha}$-L-rhamnoside(1). Compound 1 demonstrated a strong inhibition on the α-glucosidase, in vitro and ;${\alpha}$-glucosidase inhibitory value was calculated as 98.08%, when that of a reference drug, acarbose was estimated as 83.03%. The present study indicates compound 1 could be considered as an ;${\alpha}$-glucosidase inhibitor and developed as an important antidiabetes agent for type II diabetes therapy.

마디풀 추출물의 세포 보호 효과 및 주성분 분석 (Protective Effects of Cellular Membrane and Component Analysis of Polygonum aviculare Extracts)

  • 박수남;김민지;김수지
    • 한국미생물·생명공학회지
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    • 제42권1호
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    • pp.51-57
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    • 2014
  • 본 연구에서 마디풀 추출물의 항산화 활성을 평가한 결과, DPPH법과 화학발광법에 의하여 마디풀 추출물의 에틸 아세테이트 분획과 아글리콘 분획은 강력한 항산화제로 알려진 (+)-${\alpha}$-tocopherol과 $\small{L}$-ascorbic acid 보다도 우수한 항산화 효능을 나타내었다. 세포막 보호 효과 또한 연구되었다. 마디풀 추출 에틸 아세테이트 분획과 아글리콘 분획 모두 지질과산화 연쇄반응의 차단제인 (+)-${\alpha}$-tocopherol (${\tau}_{50}=38min$)에 비교하여 더 우수한 세포 보호 활성을 나타냄을 확인하였다(${\tau}_{50}=314.7min$ and 264.6 min at $10{\mu}g/ml$). 마디풀 추출물의 TLC, HPLC, LC/ESI-MS/MS, 그리고 NMR을 이용하여 주성분을 분석한 결과, 마디풀 추출물의 에틸 아세테이트 분획의 주성분은 myricitrin, avicularin, quercitrin으로 확인되었으며 마디풀 추출물의 아글리콘 분획의 주성분은 myricetin, quercetin, kaempferol로 확인되었다. 이상의 결과들로 다양한 종류의 플라보노이드를 함유한 마디풀 추출물은 에틸 아세테이트와 아글리콘 분획에서 여러가지 활성산소종에 대한 총항산화능과 $^1O_2$으로 유도된 적혈구 파괴에 대한 세포 보호 효과가 큰 것으로 나타났다. 이는 마디풀 추출물이 기능성 화장품 원료로서의 응용 가능성이 있음을 시사한다.