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Bio-assay Guided Isolation and Identification of α-Glucosidase Inhibitors from the Leaves of Diospyros lotus  

Kim, Sang Jun (Jeonju Biomaterials Institute)
Kim, Ji-Ae (Jeonju Biomaterials Institute)
Kim, Da Hye (Jeonju Biomaterials Institute)
Kwak, Seol Hwa (Jeonju Biomaterials Institute)
Yu, Kang-Yeol (Jeonju Biomaterials Institute)
Jang, Seon Il (Department of Health & Science, Jeonju University)
Kim, Seon-Yeong (Ato Q&A Corporation)
Jeong, Seung-Il (Jeonju Biomaterials Institute)
Publication Information
Korean Journal of Pharmacognosy / v.46, no.2, 2015 , pp. 105-108 More about this Journal
Abstract
To establish the anti-diabetic(α-glucosidase inhibitory) activity of D. lotus leaf extract, isolate and identify the constituents responsible for the activity. The methanolic extract of leaves was partitioned between water, n-butanol and ethyl acetate. Bio-assay guided fractionation, based on inhibition of ;${\alpha}$-glucosidase, allowed isolation and identification of the active components. Liquid chromatography/mass spectrometry(LC/MS), 1 H-NMR and 13 C-NMR spectra analyses demonstrated that the active compound was myricetin-3-O-;${\alpha}$-L-rhamnoside(1). Compound 1 demonstrated a strong inhibition on the α-glucosidase, in vitro and ;${\alpha}$-glucosidase inhibitory value was calculated as 98.08%, when that of a reference drug, acarbose was estimated as 83.03%. The present study indicates compound 1 could be considered as an ;${\alpha}$-glucosidase inhibitor and developed as an important antidiabetes agent for type II diabetes therapy.
Keywords
Diospyros lotus; ${\alpha}$-Glucosidase; Myricetin-3-O-${\alpha}$-L-rhamnoside; Acarbose; Antidiabete;
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