• 대한수의학회지
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• 제13권1호
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• pp.35-38
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• 1973

It was discovered that furamizole, one of the nitrofuran derivatives, possesses a strong antibacterial activity upon various organisms. Since then, a number of nitrofuran derivatives have been examined for antibacterial activity. This experiment was carried in vitro to evaluate with regard to antibacterial activity upon Mycoplasma gallisepticum. As a result, furamizole was found to be a good effective compound upon Mycoplasma gallisepticum. Furamizole inhibited the growths of the three strains of Mycoplasma gallisepticum and two strains of the same species at a concentration of $0.064{\mu}g/mI$. and $0.016{\mu}g/mI$. In short, there were not great differences in sensitivity to furamizole among the five strains tested.

• Genomics & Informatics
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• 제3권3호
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• pp.63-67
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• 2005

Adaptive responses to diverse microbial pathogens might be limited in relatively few types. Host cell responses to pathogens are believed to be patterned or stereotyped along with species or class. We tried to compose the host response to Mycoplasma in terms of cellular gene expression. Although gene expression profile of two host HeLa and 293 cells were quite different each other, 30 genes were differentially expressed by mycoplasma infection in both of HeLa and 293 cells. Six of them (PR48, MADH4, MKPX, CRK, RBM7, NEK3) were related to cell cycle or proliferation. Another category of genes like IL1 HY1, KLRF1, TNFSF14, GBP1 were host defense to elicit immune responses. With this set of genes, we establish the prediction model for mycoplasma contamination.

• Journal of Microbiology
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• 제44권1호
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• pp.42-49
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• 2006

In this study, we describe our newly-developed sensitive two-stage PCR procedure for the detection of 13 common mycoplasmal contaminants (M. arthritidis, M. bovis, M. fermentans, M. genitalium, M. hominis, M. hyorhinis, M. neurolyticum, M. orale, M. pirum, M. pneumoniae, M. pulmonis, M. salivarium, U. urealyticum). For primary amplification, the DNA regions encompassing the 16S and 23S rRNA genes of 13 species were targeted using general mycoplasma primers. The primary PCR products were then subjected to secondary nested PCR, using two different primer pair sets, designed via the multiple alignment of nucleotide sequences obtained from the 13 mycoplasmal species. The nested PCR, which generated DNA fragments of 165-353 bp, was found to be able to detect 1-2 copies of the target DNA, and evidenced no cross-reactivity with the generated DNA of related microorganisms or of human cell lines, thereby confirming the sensitivity and specificity of the primers used. The identification of contaminated species was' achieved via the performance of restriction fragment length polymorphism (RFLP) coupled with Sau3AI digestion. The results obtained in this study furnish evidence suggesting that the employed assay system constitutes an effective tool for the disagnosis of mycoplasmal contamination in cell culture systems.

• Clinical and Experimental Reproductive Medicine
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• 제23권3호
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• pp.311-318
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• 1996

Mycoplasmas have long been suspected of contributing to involuntary infertility in couples. However considerable disagreement exits concerning the role of genital mycoplasma infection in human infertility. Several investigators have noted abnormalities in the semen analysis of men with positive mycoplasma cultures, and early epidemiologic studies indicated that Ureaplasma urealyticum was linked to human reproductive failure on the basis of higher frequencies of isolation from infertile versus fertile couples and successful pregnancies in infertile couples after doxycycline therapy. However, subsequent investigators have questioned these findings because there are many studies in which treatment for mycoplasma in the male or female did not demonstrate an improved pregnancy rate, and semen samples from unexplained infertile men containing ureaplasmas have not revealed poorer motility, fewer spermatozoa and more aberrant forms. The objective of this study were to investigate the incidence rate of mycoplasma in semen and to investigate whether the presence of mycoplasma in semen makes significant difference to the semen volume, sperm motility and sperm counts. The results were that the rate of isolation of mycoplasma species was 70.3%. Semen volume is $2.84{\pm}1.01ml$ for culture negative and $3.15{\pm}1.42ml$ for culture positive group. Sperm motility is $46.23{\pm}15.80%$ for culture negative and $50.09{\pm}15.69%$ for culture positive group, and sperm count is $95.47{\pm}47.14({\times}(P)10^6/ml)$ for culture negative and $86.73{\pm}47.59({\times}10^6/ml)$ for culture positive group. In conclusion, we suggest that the presence of mycoplasma in semen makes no significant differences to the sperm parameters.

• 미생물학회지
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• 제41권4호
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• pp.269-274
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• 2005

동물용 생 바이러스 백신 내에 mycoplasma를 검출하기 위해 polymerase chain reaction (PCR)기법과 2가지의 상품화된 PCR 검출킷트를 평가하였다. PCR기법은 시험에 사용된 모든 mycoplasma를 특이적으로 검출할 수 있었으나, 2가지의 상품화된 PCR 검출킷트는 일부의 mycoplasma를 검출하지 못하였다. 또한, PCR기법의 검출 특이도는 조류 유래 mycoplasma에 속한 4주의 표준주 및 7주의 야외분리주를 모두 검출할 수 있었다. PCR기법의 민감도는 9 CFR Mycoplasma액체배지에서 배양한 Mycoplasma 속균 및 Acholeplasma속균에 대해 $1\~100$ colony forming units/ml까지 검출할 수 있었다. 동물용생 바이러스 백신에 대해 PCR기법의 적용가능성을 평가하기 위해, 돼지 전염성위장염 및 로타바이러스 흔합백신과 개 파보바이러스 백신내에 A. laidlawii를 인공적으로 접종한 후, PCR기법의 민감도를 조사하였을 때 배양액을 이용한 검출한계와 유사하였다. 본 연구에서 사용된 PCR 기법은 동물용 생 바이러스 백신내의 mycoplasma를 신속하고 민감하게 검출할 수 있을 것으로 판단되었다.

• 대한수의학회지
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• 제40권3호
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• pp.645-652
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• 2000

This study was performed to investigate Mycoplasma (M) spp. infection status of dairy cow in South Korea. Among 8,485 bulk tank milk collected from dairy farms of the 5 areas, 26 samples (0.30%) were positive for Mycoplasma by direct culture method. The isolation rates of Mycoplasma spp. according to the areas were 0.51% in Kyonggi, 0.16% in Cholla, 0.23% in Gyoungsang, 0.12% in Chungchong, and 0.08% in Kangwon. In the species identification test by indirect immunoperoxidase test, 16 out of 26 isolates were identified as M bovis (61.53%), M bovigenitalium (23.07%), M californicum (7.69%), M alkalescens and Acholeplasma laidlawii (3.84%), respectively. The isolation rate of Mycoplasma spp. from 208 quarter milk samples in culling cows due to severe clinical mastitis was 3.0%. These Mycoplasma spp. were identified as M bovis (62.0%), M bovigenitalium (12.0%), M californicaum (12.0%), and M alkalescens (12.0%). This study shows that the bovine mammary gland infected by Mycoplasma spp. is present in some dairy farms and the routine culture test of bulk tank milk samples for Mycoplasma is needed for a high quality milk promotion services.

• 한국가금학회지
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• 제46권4호
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• pp.249-253
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• 2019

닭 마이코플라즈마병은 전세계적으로 양계산업에 문제시 되고 있는 난계대 질병으로 병아리 품질 및 사육성적에 영향을 미친다고 알려져 있다. 본 연구는 닭 마이코플라즈마병에 대한 백신 접종을 실시하지 않은 계열화 회사의 육용 종계군을 대상으로 정기 채혈을 통해 혈청검사를 실시한 후 감염율을 확인하였고, 조사계군에서 생산된 육계 병아리에 대한 사육성적을 확인하고자 하였다. 육용종계 닭 마이코플라즈마 감염율과 그에 따른 후대병아리의 사육성적을 연도별로 확인한 결과, 종계군의 감염율이 낮아짐에 육계의 사육성적이 높아진다는 상관관계를 확인하였다. 이러한 결과를 토대로 비추어 보았을 때, 닭 마이코플라즈마병의 감염 유무는 생산된 초생추의 품질과 사육농장 성적 영향에 미치는 여러 요소들 중 하나라고 판단할 수 있다.

• 대한수의학회지
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• 제42권2호
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• pp.225-229
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• 2002

We report that mycoplasma organisms from lung tissues of slaughter pigs were identified to genes fragments with references use of nested-PCR technique(nPCR). Seven strains of mycoplasma species were isolated from 70 lung tissues. The organisms were detected by in vitro amplification of 16S rRNA and 23S rRNA genes. Nucleotide sequences of the spacer between 16S and 23S in the ribosomal RNA operons of mycoplasma were identified by the analysis of products from the nested PCR. Four common PCR primers, MhF1, MhF2 MhR1 and MhR2, were designed by analysis between these sequences by first amplified with F1, R1 and second with F2, R2, respectively. Specific amplification of the spacer region for reference strains of M. hyopneumoniae, M. hyorhinis, M. flocculare were confirmed by first round of PCR in which the traduced fragments of 690bp, 460bp, 630bp. But amplications of second round was changed to 240bp, 210bp, 230bp, respectively. Three different strains (M. hyopneumoniae:4, M. hyorhinis:2, M. flocculare:1) were detected by the nested-PCR technique. The results suggest that the detection of swine mycoplasma by n-PCR can be analyzed the nucleotide sequences between rRNA operons and homology study.

• Journal of Microbiology and Biotechnology
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• 제19권3호
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• pp.265-270
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• 2009

An oligonucleotide array was developed to detect and genotype mollicutes based on the internal transcribed spacer (ITS) sequence. The results of the assay were compared with those of a PCR-RFLP assay. The proposed oligonucleotide array containing 5 genus- and 23 species-specific probes was able to detect Mycoplasma species, including M. penetrans and M. spermatophilum, that were not detected by the PCR-RFLP assay. Therefore, the results demonstrated that the proposed oligonucleotide array was effective for the detection and discrimination of 23 species, including an acholeplasma, 21 mycoplasmas, and a ureaplasma, and showed promise as a countermeasure to ensure that biological products are safe and of good quality.

• Tuberculosis and Respiratory Diseases
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• 제45권3호
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• pp.636-642
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• 1998

저자들은 항마이코플라스마 항체와 한냉 응집소 검사로 진단된 마이코플라스마 폐렴에서 급성 호흡곤란 증후군과 한냉 응집소용혈성 빈혈, 그리고 간염이 발생된 전격성 M. þneumoniae 감염이 스테로이드 투여로 임상 증상 및 X-선 소견 그리고 한냉 응집소 용혈성 빈혈이 호전되었던 1예를 치험하였기에 문헌 고찰과 함께 보고하는 바이다.