• Title/Summary/Keyword: Mycological characteristics

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Gray Mold of Primula Caused by Botrytis cinerea (Botrytis cinerea에 의한 프리뮬러 잿빛곰팡이병)

  • 권진혁;박창석
    • Research in Plant Disease
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    • v.8 no.1
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    • pp.66-69
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    • 2002
  • In February of 2002, the gray mold disease occurred severely on primular(primular spp.) grown in a flower nursery farm in Jinju, Korea. At first, the infected leaves were discolored to gray or dark from the tip and gradually expanded to die. The fungus attacked not only leaves but petals, calyx flower stalk and stems. Conidia of the fungus in mass were hyaline or gray, 1-celled, mostly ellipsoid or ovoid and sized 6.8~16.9$\times$4.6~12.4 $\mu$m. Conidiophores were wide of 15.9~31.6$\mu$m in size. The sclerotia were readily formed potato-dextrose agar and optimum temperature far sclerotial formation was 2$0^{\circ}C$. Pathogenecity of the causal organism was and preyed according to Koch\`s postulate. The causal organism was identified as Botrytis cinerea based on mycological characteristics. This is the first report on gray mold of primula caused by Botrytis cinerea in Korea.

Stem Rot of Disporum sessile Caused by Sclerotium rolfsii in Korea (Sclerotium rolfsii에 의한 윤판나물 흰비단병)

  • Kwon, Jin-Hyeuk;Shim, Chang-Ki;Park, Chang-Seuk
    • Research in Plant Disease
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    • v.13 no.1
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    • pp.50-52
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    • 2007
  • From 2004 to 2005, the stem rot of Disporum sessile D. $D_{ON}$ caused by Sclerotium rolfsii occurred spoyadieally in an herbs exhibition farm of Hamyang, Gyeongsangnam-do Agricultural Research and Extension Services, Korea. The typical symptom is water-soaking, dark brown on the stem and rotting, wilting. The infected plants were mostly died. White mycelial mats were spread over lesions, and then sclerotia were formed on stems and near soil line. The sclerotia were globoid in shape, 1$\sim$3 mm in size and white to brown in color, The optimum temperature and sclerotia on PDA was 30$^{\circ}C$ and the hyphal width was $3{\sim}10{\mu]m$. The typical clamp connections were observed in the hyphae of the fungus grown on PDA. Pathogenicity of the causal organism was proved on D. sessile according to Koch's postulate. On the basis of mycological characteristics and pathogenicity to host plants, this fungus was identified as Sclerotium rolfsii Saccardo. This is the first report on the stem rot of D. sessile caused by S. rolfsii in Korea.

Occurrence of Fruit Rot of Pumpkin Caused by Sclerotium rolfsii (Sclerotium rolfsii에 의한 호박 흰비단병 발생)

  • Kwon, Jin-Hyeuk;Shen, Shun-Shan;Chae, Yun-Seok;Park, Chang-Seuk
    • Research in Plant Disease
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    • v.13 no.1
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    • pp.53-56
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    • 2007
  • The fruit rot of pumpkin caused by Sclerotium rolfsii occurred sporadically at Gyeongsangnam-do Agricultural Research and Extension Services, Korea in 2004 and 2005. The typical symptom of the disease included water-soaking, fruit rotting and wilting. White mycelial mats were spread over infected fruits and then sclerotia were formed on the lesions and near soil surface. The sclerotia were globoid in shape, 1$\sim$3 mm in size and white to brown in color. The optimum temperature for mycelial growth and sclerotia formation was at $30^{\circ}C$ on PDA and the hyphal width ranged from 4 to 8 ${\mu}m$. The typical clamp connections were observed in the hyphae of the fungus grown on PDA. On the basis of mycological characteristics and pathogenicity to host plants, this fungus was identified as Sclerotium rolfsii Saccardo. This is the first report on the fruit rot of Cucurbita spp. caused by S. rolfsii in Korea.

Anthracnose of Salvia plebeia Caused by Colletotrichum acutatum in Korea (Colletotrichum acutatum에 의한 배암차즈기 탄저병)

  • Kwon, Jin-Hyeuk;Nam, Myeong-Hyeon;Park, Chang-Seuk
    • Research in Plant Disease
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    • v.13 no.2
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    • pp.119-121
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    • 2007
  • Anthracnose of Salvia plebeia caused by Colletotrichum acutatum was observed in the exhibition field of Hamyang-gun, Gyeongsangnam-do Agricultural Research and Extension Services, in Korea. The disease mainly occurred on the leaves, petioles and stems of whole plant. The typical symptoms were water-soaked lesions on stems and petioles, rotted stems and withering then eventually died. The color of colonies of the pathogenic fungus grown on potato dextrose agar was pinkish gray. Conidia were fusiform in shape and was $8{\sim}17{\times}3{\sim}4\;{\mu}m$ in size. Appressoria were pale to dark brown in color, clavate in shape and the sizes were $8{\sim}11{\times}4{\sim}6\;{\mu}m$. Optimum temperature for mycelial growth was $25^{\circ}C$. On the basis of mycological characteristics and pathogenicity test on host plants, the fungus was identified as Colletotrichum acutatum J. H. Simmonds. This is the first report on the anthracnose of S. plebeia caused by C. acutatum in Korea.

Sclerotium Rot of Cyclamen europaeum Caused by Sclerotium rolfsii (Sclerotium rolfsii에 의한 시클라멘 흰비단병)

  • Kwon, Jin-Hyeuk;Lee, Heung-Su;Kim, Jinwoo;Kim, Won-Il;Shim, Hong-Sik;Shen, Shun-Shan
    • Research in Plant Disease
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    • v.20 no.3
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    • pp.223-226
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    • 2014
  • Sclerotium rot caused by Sclerotium rolfsii occurred on Cyclamen europaeum grown at the experimental greenhouse of Gyeongsangnam-do Agricultural Research and Extension Services in April 2013. Infected plants showed water-soaked appearance, wilting, and rotting; severely infected plants eventually died. White mycelial mats spread over lesions, and sclerotia were formed on leaves, petioles, and flower stalks near soil line. On the basis of mycological characteristics, ITS rDNA sequence analysis, and pathogenicity to host plants, this fungus was identified as S. rolfsii Saccardo. This is the first report of sclerotium rot on C. europaeum caused by S. rolfsii in Korea.

Occurrence of Gray Mold on Astragalus sinicus L. Caused by Botrytis cinerea in Korea (Botrytis cinerea에 의한 자운영 잿빛곰팡이병의 발생)

  • Kwon, Jin-Hyeuk;Lee, Heung-Su;Oh, In-Seok
    • Research in Plant Disease
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    • v.15 no.2
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    • pp.127-129
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    • 2009
  • Gray mold caused by Botrytis cinerea occurred on Astragalus sinicus in an experimetal rice field of Gyeongsangnam-do Agricultural Research and Extension Services in Korea from 2008 to 2009. The symptoms appeared on the leaves, petiole and stems of infected plants. and then started with water-soaked, rotted and eventually died blight on plants. The colony were grayish brown and the optimum temperature for sclerotial formation was $20^{\circ}C$ on potato dextrose agar. The conidia were one celled, mostly ellipsoid or ovoid in shape, and were colorless or pale brown in color. The conidia were $7{\sim}16{\times}5{\sim}10{\mu}m$ in size and conidiophores were $14{\sim}28{\mu}m$ in length. On the basis of mycological characteristics and pathogenicity test on host plants, the fungus was identified as Botrytis cinerea Persoon: Fries.

Pink Mold Rot on Asian Pear (Pyrus serotina Rehder) Caused by Trichothecium roseum (Pers.) Link ex Gray in Korea (Trichothecium roseum에 의한 배 분홍빛썩음병 발생)

  • Kwon, Jin-Hyeuk;Lee, Heung-Su;Choi, Si-Lim;Cho, Cho-Yong;Choi, Ok-Hee;Cho, Hyeoun-Suk;Shim, Chang-Ki
    • Korean Journal of Organic Agriculture
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    • v.21 no.3
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    • pp.373-380
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    • 2013
  • A severe pink mold rot on matured asian pear (Pyrus serotina Rehder) fruit occurred in the organic farmers' orchard in Jinju, Korea in October, 2012. Decay of pear fruit appeared as a softened water-soaked symptom that was easily punctured by pressure. Later pink mycelium appeared on the surface of pear fruit and produced a mass of powdery pink conidia spores. Optimum temperature for mycelial growth of T. roseum was $25^{\circ}C$. Conidia showed hyaline, smooth, 2-celled, thick-walled with truncate bases, ellipsoidal to pyriform, and characteristically held together zig-zag chains and $10{\sim}22(34){\times}6{\sim}10(12){\mu}m$ in size. Conidiophore was erect, colorless, unbranched type, and 4-5 ${\mu}m$ width. On the basis of mycological characteristics, pathogenicity test, and molecular identification with the ITS region, the causal fungus was identified as Trichothecium roseum (Pers.) Link ex Gray.

Stem Rot of Perilla Caused by Sclerotium rolfsii in Korea (Sclerotium rolfsii에 의한 들깨 흰비단병)

  • Kwon, Jin-Hyeuk;Choi, Si-Lim;Lee, Heung-Su;Shim, Hong-Sik
    • The Korean Journal of Mycology
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    • v.40 no.3
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    • pp.177-178
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    • 2012
  • Stem rot of perilla (Perilla frutescens var. japonica Hara) occurred sporadically in the exhibition field at Gyeongsangnam-do Agricultural Research and Extension Services in Korea. The infected stems are water-soaked, rotted, wilted and finally leads to the death of the whole plants. The sclerotia, 1-3 mm, white to brown, spherical, were formed on lesions and the surface of soil line. The optimum temperature for mycelial growth and sclerotia formation was $30^{\circ}C$ on potato dextrose agar and the hyphal width was 4-9 ${\mu}m$. The typical clamp connections were observed in the hyphae of the fungus. On the basis of mycological characteristics and pathogenicity to host plants, this fungus was identified as Sclerotium rolfsii Saccardo. This is the first report of stem rot on perilla caused by S. rolfsii in Korea.

Isolation and Identification of Wild Yeasts from Freshwaters and Soils of Nakdong and Yeongsan River, Korea, with Characterization of Two Unrecorded Yeasts (낙동강과 영산강 담수와 주변 토양으로부터 야생효모의 분리 및 동정)

  • Han, Sang-Min;Kim, Ha-Kun;Lee, Hyang-Burm;Lee, Jong-Soo
    • The Korean Journal of Mycology
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    • v.44 no.4
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    • pp.350-354
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    • 2016
  • Diverse wild yeast were isolated from freshwaters and soils of Nakdong and Yeongsan rivers in Korea and identified by the comparison of polymerase chain reaction-amplified nucleotide sequences of the internal transcribed spacer region (including the 5.8S rRNA) and D1/D2 regions of 26S rDNA, using BLAST. In total, 15 strains belonging to 9 species were isolated from 25 samples, out of which Aureobasidium pullulans and Cryptococcus bestiolae were dominant. Candida ghanaensis JSF0127 and Meira geulakonigii JSF0130 were identified as unrecorded yeasts, for which their mycological characteristics were investigated. These unrecorded yeasts formed ascospores and grew in yeast extract peptone dextrose medium containing 5% NaCl.

Sclerotium rolfsii Causing Collar Rot on Doonggulle (Polygonatum odoratum var. pluriflorum) (둥굴레에 흰비단병을 일으키는 Sclerotium rolfsii에 관하여)

  • Kwon, Jin-Hyeuk;Kang, Soo-Woong;Park, Chang-Seuk
    • The Korean Journal of Mycology
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    • v.28 no.2
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    • pp.121-122
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    • 2000
  • A destructive collar rot of Doonggulle (Polygonatum odoratum var. pluriflorum) occurred in an experimental farm at Hamyang Medicinal Plant Experiment Station, Kyonguam ARES in 1999. The disease also observed in farmer's fileds in Hamyang and the infection rate reached up to 12.3%. Upper parts of the infected plants were mostly blighted and white mycelial mats were formed on the lesions or soil lines near the stem. The fungus readily formed sclerotia on PDA and then shapes were globoid or irregular, and sized $0.9{\sim}6.8{\times}0.6{\sim}5.2\;mm$ (av. $2.7{\sim}2.3\;mm$). Although the fungus showed wide range of mycelial growth temperature, the optimum temperature was about $30^{\circ}C$. The typical sclerotium type and clamp connections of hyphae were found in the fungus grown in PDA. The causal fungus was identified as Sclerotium rolfsii on the basis of mycological characteristics and pathogenicity that on the host plants. This is the first report on the collar rot of Polygonatum odoratum caused by Sclerotium rolfsii in Korea.

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