• International Journal of Industrial Entomology and Biomaterials
• /
• v.8 no.1
• /
• pp.61-66
• /
• 2004

Bacillus thuringiensis 656-3 which was isolated from a soil sample of mushroom house and showed high toxicity to mushroom flies, Lycoliella mali and Coboldia fuscipes, was surveyed for insecticidal effect in the oyster mushroom house.3. thutingiensis 656-3 was mass-cultured in the fermenter containing soybean cake(2%) and wheat bran (2%) as media source. Semi-for-mutation of B. thuringiensis 656-3 was performed with metamorphic starch only. When the formulation suspension containing $5{\times}{10^7}$ cfu was sprayed on the mushroom in mushroom house, the insecticidal effect of B. thuringiensis 656-3 to mushroom flies,1. mali and C. fuscipes, was maintained over 90% by the fifth day after starting spraying. The yield of oyster mushroom house with B. thuringiensis 656-3 was significantly increased compared to control. B. thuringiensis 656-3 represents a powerful biological insecticide for the control of mushroom flies.

• Journal of Mushroom
• /
• v.15 no.1
• /
• pp.14-20
• /
• 2017

The oyster mushroom cultivation house typically has multiple layers of growing shelves that cause the disturbance of air circulation inside the mushroom house. Due to this instability in the internal environment, growth distinction occurs according to the area of the growing shelves. It is known that minimal air circulation around the mushroom cap facilitates the metabolism of mushrooms and improves their quality. For the purpose of this study, a CFD analysis FLUENT R16 has been carried out to improve the internal environment uniformity of the oyster mushroom cultivation house. It is found that installing a section of the working passage towards the ceiling is to maintain the internal environment uniformity of the oyster mushroom cultivation house. When all the environment control equipment - including a unit cooler, an inlet fan, an outlet fan, an air circulation fan, and a humidifier - were operated simultaneously, the reported Root Mean Square (RMS) valuation the growing shelves were as follows: velocity 23.86%, temperature 6.08%, and humidity 2.72%. However, when only a unit cooler and an air circulation fan operated, improved RMS values on the growing shelves were reported as follows: velocity 23.54%, temperature 0.51%, and humidity 0.41%. Therefore, in order to maintain the internal environment uniformity of the mushroom cultivation house, it is essential to reduce the overall operating time of the inlet fan, outlet fan, and humidifier, while simultaneously appropriately manage the internal environment by using a unit cooler and an air circulation fan.

• Journal of The Korean Society of Agricultural Engineers
• /
• v.48 no.6
• /
• pp.31-41
• /
• 2006

The analysis used in this work was cost-benefit analysis method. All future costs and returns of a given mushroom house were discounted to the time of initial investment (present) by means of 3.5% discount rate. Then the cost of ownership was compared to the return from the system. This analysis method has been developed and coded into a balance sheet for use on a EXCEL program. Using this programmed analysis,a large number of the case studies were examined using different combinations of economic conditions. These results will be very useful to individuals considering investment in a mushroom house, or any similar production system. By the way of the sensitivity analysis for each important parameter, the change of the marginal cost-benefit period could be finally determined. These parameters were typically construction cost of mushroom house, cost of cooling system, required cooling and heating energy amounts, unit price of mushroom media bottle, growing number of media bottles, production weight per unit bottle, sale price of mushroom, and annual number of growing period, etc.

• Journal of The Korean Society of Agricultural Engineers
• /
• v.46 no.7
• /
• pp.35-44
• /
• 2004

In this study, a ventilation model was developed to determine a ventilation rate for the balance of heat, moisture and $CO_{2}$ in a mushroom house. Internal and external temperature, relative humidity and $CO_{2}$ concentration were measured and used to validate the ventilation model. The effects of various environmental factors on physiological responses of mushroom were also investigated. The verified model was simulated under the observed ventilation rates with a difference of$ 0.001{\~}0.065\;m^{3}{\cdot}S^{-1}$ (relative error of $0.3{\~}18.9\%$) when external temperature varied 22.5 to $24.8^{circ}C$ and average ventilation rates was $0.35m^{3}{\cdot}S^{-1}$. The optimal conditions for mushroom growth (internal temperature $22 ^{circ}C$, relative humidity $80\%$, $CO_{2}$ concentration 1,000 ppm) were used for the model application with external temperature, relative humidity and $CO_{2}$ concentration of $27.5{\~}33.5^{circ}C$, $60\%$, and 355 ppm, respectively. Thermal balance was a important factor for an optimum ventilation up to the external temperature of $32^{circ}C$, while $CO_{2}$ concentration balance was more important over $32^{circ}C$. This suggests that humidification for moisture balance is required to maintain temperature and $CO_{2}$ concentration at an optimal level by ventilation in a mushroom house.

• Korean Journal of Medicinal Crop Science
• /
• v.22 no.6
• /
• pp.497-503
• /
• 2014

In order to produce the high quality of dried-ear mushroom, various drying methods such as hot-air drying at $40{\sim}80^{\circ}C$, freeze drying and drying in vinyl house were carried out. Drying hours of hot-air drying, freeze drying and drying in vinyl house were 12.5~21.5, 36.0 and 72.0 hrs, respectively. Vitamin $D_2$ content of sample was the highest as $6.77{\mu}g/g$ DW in drying in vinyl house and then followed by freeze drying as $5.90{\mu}g/g$ DW and hot-air drying as $1.89{\sim}5.01{\mu}g/g$ DW. After dry, external appearance and color of mushrooms applied hot-air drying and drying in vinyl house were better than freeze-dried one. After rehydration, water uptake of sample in drying in vinyl house and hot-air drying at $50{\sim}60^{\circ}C$ were 17.8 and 19.3~21.0 times, respectively. The methods of drying in vinyl house and hot-air drying at $50{\sim}60^{\circ}C$ also led to high hardness, good shape and resilience. As the results of production of dried-ear mushroom with high quality, we suggest that the best method for drying is the drying in vinyl house due to not only high vitamin $D_2$ content, good external appearance and color after drying but also high hardness and good shape after rehydration.

• Journal of Bio-Environment Control
• /
• v.28 no.1
• /
• pp.28-37
• /
• 2019

We designed a system that can automatically collect, convey, and control cool air of $15^{\circ}C-20^{\circ}C$ containing carbon dioxide from a mushroom cultivation house to a strawberry plastic house. We recorded the temperature at various positions from July to August 2017. The average temperature of the green house during day and at night was maintained at $33^{\circ}C$ and $26^{\circ}C$, respectively. In the moveable three-tier cylindrical bed, the average temperature around root was maintained at $26^{\circ}C$ and $21^{\circ}C$ during day and at night, respectively. On the high-bench in the green house, the temperature was maintained at $32^{\circ}C$ and $30^{\circ}C$ during day and at night, respectively. The carbon dioxide concentration was maintained around 800-1,600 ppm in the mushroom cultivation system and 400-800 ppm in the strawberry plastic house. The growth characteristics of the strawberry treated with moveable three-tier cylindrical bed were significantly different from those of the untreated high-bench bed. In addition, during the summer season, moveable three-tier cylindrical bed showed more tendency to increase in normal fruit number (NFN) and to decrease in defective fruit number (DFN) compare to the high-bench bed. Therefore, the moveable three-tier cylindrical bed showed a tendency to be more than 2 times higher yields than that of the high-bench bed. It was confirmed that ever-bearing strawberry cultivars could be cultivated in green house due to the cool air supply from the mushroom cultivation system in the summer season.

• 한국생물공학회:학술대회논문집
• /
• 2003.10a
• /
• pp.19-23
• /
• 2003

Temperature conditions in the mushroom cultivating room affected color, yield, pinheading rate, growth rate and other characteristics of fruitbody. These results seemed to tell the quality of mushroom. Carbon dioxide gas generated from respiration of mushroom also made stipe length long and pilei size small. High concentration of carbon dioxide could make fruitbody abnormal or dead. Mycelial shapes in fruitbody inner tissue were different according to the part and the size of fruitbody.

• Journal of Mushroom
• /
• v.16 no.4
• /
• pp.342-346
• /
• 2018

The temperature and humidity of 49 greenhouses for oak mushroom cultivation were investigated for 5 years to analyze the trends in the change of these parameters according to the climate change in Korea. The 5-year average temperature and humidity were $24.7^{\circ}C$ and 60.5%, respectively, in sawdust media-based cultivation houses and $24.4^{\circ}C$ and 60.0%, respectively, in log-bed cultivation houses. The average temperature in the summer was $29.8^{\circ}C$ in 2016, $29.1^{\circ}C$ in 2017, and $33.3^{\circ}C$ in 2018 in the log-bed cultivation houses and $26.8^{\circ}C$ in 2016, $20.4^{\circ}C$ in 2017, and $24.2^{\circ}C$ in 2018 in the sawdust media-based cultivation houses. During the investigation, temperatures over $30^{\circ}C$ were detected in one cultivation house in spring and five such houses in summer. When classifying by cultivation type, temperatures over $30^{\circ}C$ were found in five log-bed cultivation houses and temperatures less than $20^{\circ}C$ were found in four log-bed cultivation houses in fall. This study shows that log-bed cultivation houses for oak mushroom need to be modified to cope with the climate change.

• Journal of Mushroom
• /
• v.15 no.3
• /
• pp.118-123
• /
• 2017

This study was carried out to develop the technology to manage the growth of mushrooms, which were cultivated based on long-term information obtained from quantified data. In this study, hardware that monitored and controlled the growth environment of the mushroom cultivation house was developed. An algorithm was also developed to grow mushrooms automatically. Environmental management for the growth of mushrooms was carried out using cultivation sites, computers, and smart phones. To manage the environment of the mushroom cultivation house, the environmental management data from farmers cultivating the highest quality mushrooms in Korea were collected and a growth management database was created. On the basis of the database value, the management environment for the test cultivar (hukthali) was controlled at $0.5^{\circ}C$ with 3-7% relative humidity and 10% carbon dioxide concentration. As a result, it was possible to produce mushrooms that were almost similar to those cultivated in farms with the best available technology.

• Mycobiology
• /
• v.48 no.4
• /
• pp.263-275
• /
• 2020

Phlebopus spongiosus is a well-known edible ectomycorrhizal mushroom indigenous to southern Vietnam. The mushroom specimens collected from northern Thailand in this study were identified as P. spongiosus. This identification was based on morphological characteristics and the multi-gene phylogenetic analyses. Pure cultures were isolated and the relevant suitable mycelial growth conditions were investigated. The results indicated that the fungal mycelia grew well on L-modified Melin-Norkans, and Murashige and Skoog agar all of which were adjusted to a pH of 5.0 at 30 ℃. Sclerotia-like structures were observed on cultures. The ability of this mushroom to produce fruiting bodies in the absence of a host plant was determined by employing a bag cultivation method. Fungal mycelia completely covered the cultivation substrate after 90-95 days following inoculation of mushroom spawn. Under the mushroom house conditions, the highest amount of primordial formation was observed after 10-15 days at a casing with soil:vermiculite (1:1, v/v). The primordia developed into a mature stage within one week. Moreover, identification of the cultivated fruiting bodies was confirmed by both morphological and molecular methods. This is the first record of P. spongiosus found in Thailand and its ability to form fruiting bodies without a host plant.