• Journal of Yeungnam Medical Science
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• 제20권2호
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• pp.187-196
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• 2003

The cytokines are the hormone-like proteins, which are produced in the mononuclear cells. They have many roles, such as immune mediators, cell differentiations, angiogenesis. The chemokines have chemotactic effects which control the host immune response. There were few reports about the cytokines associated with musculoskeletal tumors. From late 1980s, the cytokine studies of bone tumors such as osteosarcoma were started, but most studies for benign and malignant musculoskeletal tumors were left to be explored. To evaluate the characteristics of the cytokines in variable musculoskeletal tumors, tissues were obtained from the seven patients who visited the Yeungnam University hospital from February to July 2000. They were lipoma (1 case), parosteal osteoma (1 case), enchondroma (2 cases), pigmented villonodular synovitis (1 case), ganglion (1 case), and metastaic squamous cell carcinoma (1 case). The gene experession of the cytokines were analyzed by RNase protection assay (RPA) and reverse transcription-polymerase chain reaction (RT-PCR). The lipoma and parosteal osteoma expressed MIP-$1{\beta}$, and IP-10 genes. The two enchondromas showed different results, one expressed all of MIP-$1{\alpha}$, MIP-$1{\beta}$ and IP-10 genes but the other expressed none of above. The pigmented villonodular synovitis strongly expressed MIP-$1{\alpha}$ and IP-10 when compared with the other cases. The ganglion did not express all of the chemokines mentioned above. And the metastatic squamous cell carcinoma expressed all of the chemokines and especially IP-10 was highly expressed. Even though this study has only a few cases, these results provide a basis for the cytokine mediating network study in musculoskeletal tumors.

• Asian Pacific Journal of Cancer Prevention
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• 제14권5호
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• pp.3351-3355
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• 2013

Photodynamic therapy (PDT) is a promising cancer treatment modality that uses dye-sensitized photooxidation of biologic matter in target tissue. This study explored effects of the photosensitizer BCPD-17 during PDT for osteosarcoma. LM-8 osteosarcoma cells were treated with BCPD-17 and cell viability after laser irradiation was assessed in vitro with the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay. The effects of BCPD-17 during PDT recurrence were then examined on tumor-bearing mice in vivo. BCPD-17 had dosedependent cytotoxic effects on LM-8 osteosarcoma cells after laser irradiation which also had energy-dependent effects on the cells. The rate of local recurrence was reduced when marginal resection of mice tumors was followed by BCPD-17-mediated PDT. Our results indicated BCPD-17-mediated PDT in combination with marginal resection of tumors is a potentially new effective treatment for osteosarcoma.

• Journal of Periodontal and Implant Science
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• 제36권2호
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• pp.449-459
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• 2006

DFDBA(Decalcified freeze-dried bone allograft) is one of the allograft materials for periodontal bone regeneration. DFDBA provides an osteoconductive surface and osteoinductive factors. Therefore, DFDBA have been used successfully to regenerate the attachment apparatus during periodontal treatment. But recent studies was reported that wide variations in commercial bone bank preparations of DFDBA do exist, including the ability to induce new bone formation. DFDBA was experimental materials that was recovered, processed, tested, shipped and invoiced through Musculoskeletal Transplant Foundation. MTF(Musculoskeletal Transplant Foundation) is the world largest, non-profit, AATB(American Association of Tissue Banks) accredited tissue bank. The objective of this study was to determine the effects of serial dilutions of a DFDBA on human fetal osteoblastic cell proliferation and their potential to form and mineralize bone nodules. Human fetal osteoblastic cell line(hFOB 1.19) was cultured with DMEM and SSE($1{\mu}g/m{\ell}$,$10{\mu}g/m{\ell}$, $100{\mu}g/m{\ell}$, $1mg/m{\ell}$) at $34^{\circ}C$ with 5% CO2 in 100% humidity. Cell proliferation was significantly increased at $1mg/m{\ell}$, $100{\mu}g$, $10{\mu}g/m{\ell}$, $1{\mu}g/m{\ell}$, $100ng/m{\ell}$, $10ng/m{\ell}$, $1ng/m{\ell}$ of DFDBA after 5 days incubation (p<0.05). Alkaline Phosphatase(ALP) level was significantly increased in $100ng/m{\ell}$, $10ng/m{\ell}$, $1ng/m{\ell}$ of DFDABA(p<0.05). A quantified calcium accumulation was significantly increased at $1ng/m{\ell}$, $10ng/m{\ell}$ of MTF(p<0.05). These results indicated that DFDBA has an inductive effect on bone formation in vitro.

• 대한골관절종양학회지
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• 제9권1호
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• pp.124-129
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• 2003

목적: 슬관절부에 발생한 제 3 기 거대세포종에 대해 수술적 치료 후 임상적, 방사선학적 결과를 알아보고자 후향적 분석을 시행하였다. 대상 및 방법: 1991년 3월부터 2000년 2월까지 슬관절부에 발생한 제 3기의 거대세포종으로 수술적 치료를 받은 21명의 환자를 대상으로 하였으며 추시기간은 최단 1년 최장 9년으로 평균 5.7년이었다. 수술방법으로11명에서 병소내 소파술 및 액화 질소 냉동 요법 후 시멘트 충진술을 시행하였고, 7명에서 병소내 소파술 시행 후 냉동 요법 없이 시멘트 충진술 또는 자가골 이식술을 시행하였다. 관절면의 파괴가 심했던 3명에서는 광범위 절제술 후 재건술을 시행하였다. 결과: 첫 수술 후 기능적 평가는 우수 및 양호가 13례, 보통이 4례, 실패가 4례였다. 국소 재발이나 감염으로 인해 실패로 판정되어 재수술을 시행한 4례에서 최종 추시시 기능적 결과는 우수 3례, 보통이 1례로, 전체적으로는 우수 및 양호가 16례, 보통이 5례였다. 소파술, 냉동요법 및 시멘트 충진술을 시행한 11명의 환자 중에는 1명(9.1%)에서 재발하였으며, 1명(9.1%)에서 슬관절의 퇴행성 변화를 보였으며, 냉동요법 없이 소파술 및 시멘트 충진술 또는 골이식을 받은 환자 7명중 2명(28.6%)에서 재발하였고, 1명에서(14.5%) 슬관절의 퇴행성 변화를 보였다. 결론: 철저한 소파술 및 냉동 보조요법은 슬관절부의 제 3기 거대 세포종 치료에 있어 유용한 방법이며, 광범위 절제술 및 재건술은 병변이 거대하고 관절면이 상당히 파괴되었거나, 수술 후 국소 재발로 인해 골파괴 및 관절 침범이 심한 경우에 시행하는 것이 바람직 할 것으로 사료된다.

• 대한골관절종양학회지
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• 제11권2호
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• pp.160-167
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• 2005

목적: 거대세포종은 대부분 대퇴골 원위부 및 경골 근위부에 주로 발생하며, 병소 내 소파술에서 광범위 절제술까지 다양한 수술 방법들이 시행되고있다. 저자들은 수술이 어렵고 재발율이 높은 슬관절 부위의 거대세포종 환자에서 재발율을 포함한 치료 성적을 분석하고, 재발율과 관련된 인자를 규명하고자 하였다. 대상 및 방법: 병리학적으로 확진 되고, 고속 바(high-speed burring)와 골 시멘트를 이용한 병소내 소파술을 시행 한 41예의 슬관절 주위 거대세포종 환자를 대상으로 하였다. 재발율과 기능적 평가를 통한 치료 성적을 분석하였으며, 성별, 연령, 종양의 위치, 크기, 연골 하골의 침범과 관절 내 침범 유무 그리고 Campanacci 분류에 따른 인자들과 재발율과의 상관관계를 통계적으로 분석하였다. 평균 추시 기간은 50(12~122) 개월이었다. 결과: 최종 추시 상 재발율은 17%였고, 재발시기는 평균 술 후 10개월이었다. Musculoskeletal Tumor Society (MSTS) Grading System에 의한 술 후의 기능 평가점수는 평균 27.8(93%)로 78%에서 만족스러운 기능을 보였다. 성별, 연령, 종양의 위치, 크기, 연골 하침범, 관절 내 침범, Campanacci 분류 등은 종양의 국소 재발과 통계적으로 유의한 상관 관계를 보이지 않았다. 결론: 슬관절 주변 거대세포종의 재발과 관련된 인자를 찾지는 못하였다. 수술적 치료로서 고속 바를 이용한 세심한 소파술과 골시멘트 충진술을 이용한 국소 절제술 만으로도 만족할 만한 결과 및 재발율을 얻을 수 있었다.

• Asian Pacific Journal of Cancer Prevention
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• 제16권15호
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• pp.6451-6455
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• 2015

Background: Primary bone neoplasms are rare, contributing only 0.2% of the global burden of all human malignancies. Osteosarcoma (OS) and chondrosarcoma (CS) are the most common malignancies of bone. The giant cell tumor of bone (GCTb) is a benign tumor with behavior characterized by osteolytic bone destruction. The OS, CS and GCTb affect both sexes, all races and generally have incidence peaks regarding the age of the patient which vary according to the tumor type. We analyzed the incidences of OS, CS and GCTb and their relations with gender and age in patients treated in the National Rehabilitation Institute (INR, for its acronym in Spanish) over a period of nine years. Materials and Methods: In the study period, clinic pathological data for 384 patients were obtained with clinical, radiological and histopathological diagnosis for OS, GCTb and CS. Data analysis was performed using the chi-square and Fisher's exact tests. Results: From 2006 to 2014 were recorded 384 cases of bone malignancies in the database of INR. The GCTb had the highest incidence (53.1%), followed by OS (31.3%) and finally the CS (15.6%). The overall average age was $33.6{\pm}15.8$ years and the overall frequency of gender had a ratio of 1/1.03 male/female. The states with the highest incidence were Distrito Federal and Estado de Mexico with 29.2% and 25.3% respectively. Malignant neoplasms of bone assessed in the course of nine years show three significant increases in 2008, 2011 and 2014 (p=0.14). We found association between sex and tumor type (p=0.03), GCTb and CS predominated in females (54.9% and 56.6% respectively), while for the OS males were most affected (59.1%). Age was different in relation with tumor type (p=0.0001), average age was $24.3{\pm}11.2$ years for OS, $34.5{\pm}13$ years for GCTb and $49.2{\pm}18.5$ years for CS. Furthermore, associations of tumor type with topographic location of the primary tumor (P=0.0001) were found. Conclusions: In this study we can see that incidence of musculoskeletal tumor in our population is continuously increasing and in nine years an approximately 200% increase of musculoskeletal tumor cases was observed.

• 대한골관절종양학회지
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• 제13권2호
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• pp.113-118
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• 2007

골반 골에 생기는 종양은 복잡한 해부학적 구조와 체부 깊숙이 위치하는 특성으로 인하여, 상당히 커진 후에야 발견되는 경우가 많아서 진단이 늦어지고, 수술이 어려워지는 경우가 많이 있다. 특히 비구 주위에 발생한 종양은 종양의 제거 후에 고관절의 기능을 유지 할 수 있는 재건술의 방법이 극히 제한되어 있고, 수술도 어려우며, 재건술의 예후 또한 예측하기 어렵다. 저자는 신장암이 골반 골 비구 주위로 단독 전이 된 환자의 광범위 종양절제 후 저온 가열 처리한 자가골 과 인공고관절로 재건 한 증례를 보고한다.

• Journal of Periodontal and Implant Science
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• 제36권2호
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• pp.435-448
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• 2006

The purpose of this study was to investigate the effects of ICB(Irradiated frozen allogenic bone, Rocky Mountain Tissue Bank, USA) and MTF(Decalcified freeze-dried bone allograft, Musculoskeletal Transplant Foundation, USA) on the cell proliferation and differentiation of human fetal osteoblasts. Human fetal osteoblasts (hFOB1) were cultured with $10\;ng/m{\ell}$of ICB and MTF. The negatvie control group was cultured with DMSO and positive control group was cultured with BMF ($2\;ng/m{\ell}$). MIT was performed to examine the viability of the cell, and alkaline phosphatase activity was analyzed to examine the mineralization. Calcium accumulation was also evaluated. ICB and MTF did not increase the rate of the cellular proliferation of hFOB1s while they enhanced ALP and calcium accumulation. The expression of osteocalcin (OC) and bone silaloprotein (BSP) increased in hFOB1 treated with ICB and MTF ($10\;ng/m{\ell}$). These results suggest that ICB and MTF stimulate osteoblastic activity of the hFOBl.

• 대한골관절종양학회지
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• 제1권1호
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• pp.1-6
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• 1995

Giant cell tumors are primary bone tumors originating from non-osteoblastic connective tissue. The sites of involvement were commonly distal femur, proximal tibia, proximal humerus, distal radius and others (including os calcis, ilium and sacrum). Giant cell tumor located around knee joint has been difficult to treat because of local recurrence following curettage with or without bone graft. Although primary resections reduce recurrence of the lesion, the joint function will be markedly impaired. Marginal excision was very often complicated by a loss of joint integrity since all the giant cell tumors occupy juxtaarticular positions. Techniques involving physical adjuncts(high speed burr and electric cauterization) have been used in the hope of decreasing the rate of local recurrence and avoiding the morbidity of primary resection. A meticulous clinical, radiological and histological evaluation is needed to choose the correct treatment, keeping in mind the possibility of recurrence after each treatment modality.

• 대한골관절종양학회지
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• 제4권2호
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• pp.94-98
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• 1998

Clear cell sarcoma of the kidney (CCSK) is a rare malignant tumor of the renal origin in childhood, distinguished from Wilms tumor by its pathologic and clinical features. Bone metastasis is one of the characteristic clinical features. The common site of metastasis of the clear cell Sarcoma of the kidney is axial skeleton including skull, spines, ribs and femur. A cases of clear cell Sarcoma of the kidney presented to us, which solely metastasized to the hand bones without metastasizing to any other tissues including axial skeleton. We report this case with review of literatures.