• 대한한방내과학회지
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• 제10권1호
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• pp.137-155
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• 1989

In order investigate the effect of Boyanghwanotang (BT) on thrombosis, Sprague-Dawley rats injected endotoxin into the caudal vein were oral administrated solid extract of BT-182. 4mg/200g (Sample A) and 364.8mg/200g (Sample B), and then observed Platelet, fibrinogen, prothrombin time and FDP. Another group injected hydrocortisone acetate into the muscular rump were adopted to observe the effect of BT on elevated blood Viscosity, administrated Sample A and Sample B, thereafter measured body weight, body temperature, whole blood viscosity, plasma viscosity, hematocrit, RBC, WBC, hemoglobin, glucose, albumin, total protein, triglyceride, cholesterol and prothrombin time. 1. Platelet was increased significantly in Sample B compared with the control group. 2. Fibrinogen of the trial groups compared with the control group was increased, and revealed a significance in Sample B. 3. Prothrombin time was decreased significantly in the trial groups compared with the control group. 4. FDP was decreased in the trial groups compared with the control group. 5. Body weight was increased significantly in Sample B after 7 days, body temperature revealed a significance in the trial groups after 5 days. 6. Whole blood viscosity and plasma viscosity of lower shear rates, both were highly decreased in the trial groupn than the control group. 7. The increase of WBC and decrease of hematocrit, RBC and hemoglobin was showed in the trial groups, and Sample B revealed a significance on the increase of WBC and decrease of RBC. 8. The increase of prothrombin time and decrease of glucose, albumin, total protein, triglyceride and cholesterol was showed in the trial group, and Sample B revealed a Significance on the increase of prothrombin time and decrease of albumin, total protein and triglyceride. 9. As compare with Sample A and Sample B, generally Sample B was highly Significant. According to the above results, it was considered that Boyanghwanotang may be the curative effects for thrombosis and elevated blood viscosity.

• 동의생리병리학회지
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• 제19권2호
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• pp.429-433
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• 2005

We investigated the effects of aqueous extract from talc for suppression in the process of cyclophosphamide-induced cystitis in the rat. The weight of urinary bladder was increased in the cyclophosphamide-injected rat compared with normal, but the increase of weight was arrested by intake of talc. More similar results showed in the uric test involving nitrate content and blood cell number and serum analysis involving the content of blood urea nitrogen and uric acid in the talc challenged rat compared with control one. More severe histological changes of urinary bladder such as edema, wall thickness, bleeding, vacuolation in mucosal epithelium were demonstrated in the rat injected with cyclophosphamide compared with normal. Fewer scores of these changes such as edema and bleeding were observed in rats treated with talc. In the immunohistochemical analysis, the expression of inflammatory-related protein examined tended to increase in the urinary bladder of cyclophosphamide-injected rat, especially COX-2 and $TNF-{\alpha}$ in mucosal epithelium and iNOS and $IL-1{\beta}$ in mucosal and muscular layer. The decline of these immunoreation were observed in the talc treated rat, significant decrease of COX-2 was detected in mucosal epithelium and iNOS in submucosal layer. These results suggest that talc may use as a useful therapeutic agent for noninfectious cystitis.

• Journal of Acupuncture Research
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• 제36권4호
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• pp.238-244
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• 2019

Background: This study aimed to investigate the protective effects of Zanthoxylum bungeanum Maxim pharmacopuncture on disuse muscle atrophy in the gastrocnemius muscle of rats. Methods: Thirty male 250 g Sprague-Dawley rats were distributed randomly into 3 groups. The left hindlimb immobilization was performed with casting tape for 2 weeks, and no treatment was given to the right hindlimb. Rats received pharmacopuncture and were injected daily on the BL57 with either 2 mL of Zanthoxylum bungeanum Maxim aqueous extract (ZM-W group), 1 mL pharmacopuncture of Zanthoxylum bungeanum Maxim ethanol extract (ZM-E group), or 2 mL normal saline (control group). After 2 weeks of immobilization, the weight of the whole gastrocnemius muscle was measured, and the morphology of both the left and the right gastrocnemius muscles were assessed by Hematoxylin and Eosin staining. To investigate the immobilization-induced muscular apoptosis, the immunohistochemical analysis of BAX and Bcl-2 was carried out. Results: ZM-W and ZM-E significantly inhibited the reduction in weight of the left gastrocnemius muscle, the reduction in the left myofibrils, and the cross-sectional area of gastrocnemius, as compared with the control. Moreover, the ZM-W and ZM-E groups showed significantly reduced immunoreactivity for BAX, and increased immunoreactivity of Bcl-2 in left gastrocnemius muscle compared with the control group. Conclusion: These results suggest that Zanthoxylum bungeanum Maxim pharmacopuncture has protective effects against immobilization-induced muscle atrophy by regulating the activity of apoptosis-associated BAX / Bcl-2 proteins in the gastrocnemius muscle.

• 대한약침학회지
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• 제22권3호
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• pp.192-199
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• 2019

Objectives: KCHO-1(Mecasin), also called Gamijakyakgamchobuja-tang originally, is a combination of some traditional herbal medicines in East Asia. This medicine has been used mainly for alleviating neuropathic pains for centuries in Korean traditional medicine. KCHO-1 was developed to treat pain, joint contracture and muscular weakness in patients with amyotrophic lateral sclerosis. This study was carried out to investigate the chronic toxicity of KCHO-1 oral administration in rats for 26 weeks. Methods: Sprague-Dawely rats were divided into four groups and 10 rats were placed in the control group and the high-dose group, respectively. Group 1 was the control group and the remaining groups were the experimental groups. In the oral toxicity study, 500 mg/kg, 1,000 mg/kg, and 2,000 mg/kg of KCHO-1 were administered to the experimental group, and 10 ml/kg of sterile distilled water was administered to the control group. Survival rate, body weight, feed intake, clinical signs, and visual findings were examined. Urinalysis, ophthalmologic examination, necropsy, organ weight, hematologic examination, blood chemical examination and histopathologic examination were performed. Results: Mortality and toxicological lesions associated with the administration of test substance were not observed in all groups. Conclusion: NOAEL(No observed adverse effect level) of KCHO-1 is higher than 2000 mg/kg/day. And, the above findings suggest that treatment with KCHO-1 is relatively safe.

• Asian-Australasian Journal of Animal Sciences
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• 제21권7호
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• pp.928-935
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• 2008

The myostatin gene of seven important meat (Beltex (Australia), Beltex$\times$Huyang (F1), Meat and Multi-Prolific Chinese Merino Fine Wool, Meat Chinese Merino Fine Wool and Dorper (South Africa)) and non-meat (Huyang and Kazak) sheep breeds was analyzed to study the genetic basis of muscular hypertrophy (double muscling) phenotype in sheep. SNPs, four in regulatory regions and several in the introns in the myostatin gene, were identified, and the former four SNPs were used for further studies. Twelve haplotypes were predicted by PHASE program, of which four main haplotypes (1, 3, 7, 9) were present in 90% of the 364 sheep in the study. Haplotypes 1-4 were mainly present in meat breeds while haplotypes 7 and 9 dominated the non-meat breeds. The association between haplotypes and average daily gain (ADG) was analyzed among 116 sheep with production data, Haplo2 (CGAA) and Haplo8 (TGAA) were identified to have significant (p<0.05) effect on ADG by the model (JMP5.1 software) taking into account the effects of breed, family background, haplotype, birth weight and sex. ADG of these haplotype groups also correlated well (r = 0.82) with hypertrophic phenotype scores. In conclusion, the mutations -956 (T$\rightarrow$C), -41 (C$\rightarrow$A) and 6223 (G$\rightarrow$A) involved in Haplo2 and 8 may be associated with the double-muscling trait by influencing myostatin function and be suitable markers in selecting meat sheep.

• 한국산학기술학회논문지
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• 제15권3호
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• pp.1548-1556
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• 2014

본 이 연구는 S시 지적장애인센터의 지적장애인 19명(남12명, 여7명)에게 10주 동안 주 1회(120분), 놀이운동프로그램 처치가 기간별(사전·사후검사) 신체구성(체중, 체지방량, 체지방률, 근육량, 세포량)과 건강관련체력[심폐지구력, 근력(악력, 배근력), 유연성(좌전굴)〕요소에 어떠한 영향을 미치는지를 알아보는데 그 목적이 있다. 자료 분석은 SPSS 18.0 프로그램을 이용하여 기술통계치를 산출하였고, 놀이운동프로그램 참여에 따른 기간별 신체구성 및 건강관련체력의 변화에 대해 paired t-test를 실시하였다. 가설검증을 위한 유의수준은 ${\alpha}=.05$로 설정하였다. 연구결과 신체구성 변화 중 유일하게 근육량에서 유의한 차이가 나타났고, 건강관련체력은 배근력, 좌악력, 우악력, 좌전굴, 심폐지구력 모두에서 유의한 차이가 나타났다. 따라서 지적장애인들의 놀이운동프로그램이 신체구성 및 건강관련체력에 긍정적인 영향을 미친 것으로 나타났으며, 이러한 결과는 성인병 예방에도 기여 할 수 있는 에너지 소비적 활동으로 활용가능하다고 본다.

• 한국축산식품학회지
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• 제28권1호
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• pp.39-44
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• 2008

본 연구는 재래흑돼지의 육질특성과 생산성 구명을 통해 소비 및 생산 활성화를 위한 기초자료로 활용할 목적으로 사료내 에너지(ME, kcal/kg)사이신(%)수준을 달리한 3처리구, 즉 상(H), 중(M), 하(L)로 나누어 사양시험을 한 후 성장 및 육질평가를 실시하였다. 본 연구결과, 사양시험기간 동안 재래흑돼지에 있어 에너지아이신 수준이 높을수록 일당증체량과 사료요구율이 향상되는 경향을 보이고 출하일령이 짧아지는 경향을 보였다(p<0.05). 도체성적의 경우 도체율, 등지방두께, 배최장근단면적, 살코기 생산량에서 처리구간에 유의적 차이를 보이지 않았으나(p>0.05), 거세돈이 암퇘지에 비해 등지방이 두꺼운 것으로 나타났다(p<0.05). 재래흑돼지 등심육의 일반조성분, 근내지방함량, 근절길이 및 근섬유 비율은 처리구별로 유의적 차이는 없었으나, 근내지방은 영양수준이 높은 H 처리구가 M, L 처리구보다 포화지방산은 높고, 불포화지방산은 낮게 나타났으며, n-3비율은 에너지/라이신 수준이 높아질수록 낮았으나, n-6/n-3 비율은 영양수준이 높아질수록 낮아지는 경향을 보였다. 근절길이 및 근섬유 비율도 유의적인 차이는 없었으나 영양수준이 증가됨에 따라 근절길이는 다소 증가하는 경향을 보였고, 적색근 비율은 L처리구가, 백색근 비율은 M 처리구가 각각 다른 처리구보다 높았다.

• 대한정형도수물리치료학회지
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• 제12권1호
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• pp.16-26
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• 2006

The purpose of this study was to investigate the influence of the various knee angles and ground state on the muscular activities and fatigue of the ankle muscles by integrated electromyograms (iEMG) and median frequency of tibialis anterior (TA), peroneus longus (PL), flexor digitorum longus (FDL) and gastrocnemius (GC). Ten healthy male subjects were participated into stable and balance ball sessions at four angles of knee joint. The surface electromyograms (sEMG) were recorded from the TA, PL, FDL and GC on stable and balance ball with full weight bearing at four knee angles of $0^{\circ}$, $15^{\circ}$, $30^{\circ}$ and $45^{\circ}$. The time serial data of the surface electromyographic signals were transformed into integrated and frequency serial data by fast fourier transformation. On the stable ground, the iEMG signals of the TA, PL, FDL and GC were significantly higher at $45^{\circ}$ and $30^{\circ}$ of knee angles than $0^{\circ}$ and $15^{\circ}$ of knee flexion (p<0.05). On the balance ball, the iEMG of the TA, PL, FDL and GC were significantly higher at $45^{\circ}$ and $30^{\circ}$ of knee angles than $0^{\circ}$ and $15^{\circ}$ of knee flexion (p<0.05). The median frequency of the TA, PL, FDL and GC were significantly lower at $45^{\circ}$ and $30^{\circ}$ of knee angles than $0^{\circ}$ and $15^{\circ}$ of knee on the stable ground (p<0.05). On the balance ball, also the median frequency of the TA, PL, FDL and GC were significantly lower at $45^{\circ}$ and $30^{\circ}$ of knee angles than $0^{\circ}$ and $15^{\circ}$ of knee flexion (p<0.05). The iEMG of the TA, PL, FDL and GC were significantly higher on the balance ball at $0^{\circ}$, $15^{\circ}$, $30^{\circ}$ and $45^{\circ}$ of knee angles compared with stable ground. The median frequency of the TA, PL, FDL and GC were significantly lower on the balance ball at $0^{\circ}$, $15^{\circ}$, $30^{\circ}$ and $45^{\circ}$ of knee angles compared with stable ground. These results indicate that the ground conditions and angles of the knee joint involved to muscular activities and fatigue of ankles muscles, may performed at first on stable ground and then balance ball in order to $0^{\circ}$, $15^{\circ}$, $30^{\circ}$ and $45^{\circ}$ of knee flexion.

• 한국축산식품학회지
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• 제28권4호
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• pp.451-456
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• 2008

본 연구는 CLA를 육계사료에 첨가하여 그 급여수준(0, 0.5, 1.0, 1.5, 및 2.0%)에 따라 육계의 생산성과 CLA를 급여한 계육의 도체특성, 혈청콜레스테롤 및 지방산조성의 변화를 검토하고자 육계 200수(Arbor Acre Broiler, male)를 5주 동안 공시하였다 육계의 생산성을 나타내는 증체량, 사료섭취량 및 사료요구율은 CLA급여에 따른 유의성은 없었다. 도체특성중 도체율뿐만 아니라 도체중에 대한 간, 대퇴부위, 흉심 및 북채의 비율은 CLA를 급여함으로서 유의한 변화가 없었지만, 복강지방 비율은 CLA 급여량이 많아짐에 따라 유의한 감소 현상을 확인할 수 있었다(p<0.05). 혈청콜레스테롤은 CLA급여량이 증가할 수록 총콜레스테롤과 LDL-C은 감소하고, HDL-C은 증가하였다(p<0.05). 그리고 지방산 조성 변화는 CLA를 급여함에 따라 palmitic acid는 증가하고, oleic acid, linoleic acid 및 arachidonic acid는 감소하여 포화지방산은 증가하고 불포화지방산은 감소하는 현상을 보였다. 또한, CLA의 이성체함량도 CLA급여량이 증가함에 따라 직선적으로 증가하였다(p<0.05). 결론적으로 계육에 CLA 축적을 극대화하기 위해서는 2%까지 급여도 가능하지만. 지방산조성의 변화가 심하므로 CLA도 축적시키고 지방산 변화도 최소화할 수 있는 1%의 급여수준이 적합할 것으로 판단된다.

• 한국노년학
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• 제30권3호
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• pp.793-802
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• 2010

열 스트레스는 건강의 위험요인으로 장시간 노출시 열손상과 근기능 감소 등 신체 기능을 감소시키며 특히 생리적 기능이 감소되어 있는 노인에서 영향을 받기 쉽다. 본 연구는 여름철 폭염주의보 수준의 열 스트레스가 여성노인들의 근기능 및 근손상 지표에 미치는 영향을 살펴보고자 시도되었다. 65세 이상의 여성 자원자 총 8명을 대상으로 2주일 간격으로 상온(20℃) 그리고 폭염주의보 수준의 고온(33℃)에서 등속성 슬관절 근기능을 측정하였으며, 폭염주의보 수준의 열 스트레스가 근손상에 미치는 영향을 알아보기 위하여 근기능 측정 전과 후에 채혈을 실시하였다. 각속도 60°/sec 운동시, 고온(33℃) 환경에서 우측 굴곡근과 신전근에서 최대우력, 단위체중당 최대우력, 평균파워 그리고 단위체중당 평균파워에서 근기능이 감소하였다(p<0.05). 또한 각속도 180 °/sec 운동시, 고온(33℃) 환경에서 우측 슬관절 신전근 피로도가 증가하였으며(p<0.05), 굴곡근 총일량은 유의하게 증가하였다(p<0.05). 고온환경(33℃)에서 근손상 지표인 HSP70, CK 그리고 LDH는 상온(20℃)과 비교하여 유의하게 증가하였다(p<0.05). 결론적으로 열 스트레스가 여성노인들의 근기능을 감소시키고 근손상을 유발하는 것으로 나타났다. 따라서 여성노인들의 건강유지를 위하여 여름철 폭염환경에서 열 스트레스의 노출 및 신체활동을 최소화 시키는 방안이 효과적이라 사료된다.