Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
권호 표지
DOI QR코드

DOI QR Code

Association of SNP Haplotypes at the Myostatin Gene with Muscular Hypertrophy in Sheep

  • Gan, S.Q. (College of Animal Science and Technology, Shihezi University) ;
  • Du, Z. (State Key Laboratories for Agrobiotechnology, College of Biological Science, China Agriculture University) ;
  • Liu, S.R. (College of Animal Science and Technology, Shihezi University) ;
  • Yang, Y.L. (Key Labs of Sheep Breeding and Reproduce, Xinjiang Agroreclamation Academy of Sciences) ;
  • Shen, M. (College of Animal Science and Technology, Shihezi University) ;
  • Wang, X.H. (Key Labs of Sheep Breeding and Reproduce, Xinjiang Agroreclamation Academy of Sciences) ;
  • Yin, J.L. (Key Labs of Sheep Breeding and Reproduce, Xinjiang Agroreclamation Academy of Sciences) ;
  • Hu, X.X. (State Key Laboratories for Agrobiotechnology, College of Biological Science, China Agriculture University) ;
  • Fei, J. (State Key Laboratories for Agrobiotechnology, College of Biological Science, China Agriculture University) ;
  • Fan, J.J. (Ziniquan Sheep Research Institute) ;
  • Wang, J.H. (Ziniquan Sheep Research Institute) ;
  • He, Q.H. (Ziniquan Sheep Research Institute) ;
  • Zhang, Y.S. (Ziniquan Sheep Research Institute) ;
  • Li, N. (College of Animal Science and Technology, Shihezi University)
  • Received : 2007.08.22
  • Accepted : 2008.02.27
  • Published : 2008.07.01
Download PDF
⟨ Previous Next ⟩

Abstract

The myostatin gene of seven important meat (Beltex (Australia), Beltex$\times$Huyang (F1), Meat and Multi-Prolific Chinese Merino Fine Wool, Meat Chinese Merino Fine Wool and Dorper (South Africa)) and non-meat (Huyang and Kazak) sheep breeds was analyzed to study the genetic basis of muscular hypertrophy (double muscling) phenotype in sheep. SNPs, four in regulatory regions and several in the introns in the myostatin gene, were identified, and the former four SNPs were used for further studies. Twelve haplotypes were predicted by PHASE program, of which four main haplotypes (1, 3, 7, 9) were present in 90% of the 364 sheep in the study. Haplotypes 1-4 were mainly present in meat breeds while haplotypes 7 and 9 dominated the non-meat breeds. The association between haplotypes and average daily gain (ADG) was analyzed among 116 sheep with production data, Haplo2 (CGAA) and Haplo8 (TGAA) were identified to have significant (p<0.05) effect on ADG by the model (JMP5.1 software) taking into account the effects of breed, family background, haplotype, birth weight and sex. ADG of these haplotype groups also correlated well (r = 0.82) with hypertrophic phenotype scores. In conclusion, the mutations -956 (T$\rightarrow$C), -41 (C$\rightarrow$A) and 6223 (G$\rightarrow$A) involved in Haplo2 and 8 may be associated with the double-muscling trait by influencing myostatin function and be suitable markers in selecting meat sheep.

Keywords

References

  1. Banks, R. 1997. The Meat Elite Project: establishment and achievements of an elite meat sheep nucleus. Proc. Assoc. Adv. Anim. Breed. Genet. 12:598-601.
  2. Busboom, J. R., T. I. Wahl and G. D. Snowder. 1999. Economics of callipyge lamb production. J. Anim. Sci. 77 (Suppl. 2):243-248. https://doi.org/10.2527/1999.77suppl_2243x
  3. Chu, M. X., C. T. Xiao, Y. Fu, L. Fang and S. C. Ye. 2007. PCR-SSCP polymorphism of inhibin $\beta$ A gene in some sheep breeds. Asian-Aust. J. Anim. Sci. 20:1023-1029. https://doi.org/10.5713/ajas.2007.1023
  4. Cieslak, D., T. Blicharski, W. Kapelanski and M. Pierzchala. 2003. Investigation of polymorphisms in the porcine myostatin (GDF8; MSTN) gene. Czech J. Anim. Sci. 48:69-75.
  5. Clop, A., F. Marcq, H. Takeda, D. Pirottin and X. Tordoir. 2006. A mutation creating a potential illegitimate microRNA target site in the myostatin gene affects muscularity. Nat. Genet. 38:813-818. https://doi.org/10.1038/ng1810
  6. Du, R., Y. F. Chen, X. R. An, X. Y. Yang, Y. Ma, L. Zhang and X. L. Yuan. 2005. Cloning and sequence analysis of myostatin promoter in sheep. DNA Seq. 16:412-417. https://doi.org/10.1080/10425170500226474
  7. Dunner, S., M. E. Miranda and Y. Amigues. 2003 Haplotype diversity of the myostatin gene among beef cattle breeds. Genet. Sel. Evol. 35:103-118. https://doi.org/10.1186/1297-9686-35-1-103
  8. Gu, Z. L., H. F. Zhang, D. H. Zhu and H. Li. 2002. Single nucleotide polymorphism analysis of the chicken myostatin gene in different chicken lines. Acta Genet. Sin. 29:599-632.
  9. Jiang, Y. L., N. Li, X. Z. Fan, L. R. Xiao, R. L. Xiang, X. X. Hu, L. X. Du and C. X. Wu. 2002. Associations of T$\rightarrow$A mutation in the promoter region of myostatin gene with birth weight in Yorkshire pigs. Asian-Aust. J. Anim. Sci. 15:1543-1545. https://doi.org/10.5713/ajas.2002.1543
  10. Kambadur, R., M. Sharma, T. P. L. Smith and J. J. Bass. 1997. Mutations in myostatin (GDF8) in Double-Muscled Belgian Blue and Piedmontese cattle. Genome Res. 7:910-915. https://doi.org/10.1101/gr.7.9.910
  11. Li, X. L., Z. L. Wu, Y. F. Gong, Y. Q. Liu, Z. Z. Liu, X. J. Wang, T. R. Xin and Q. Ji. 2006a Single-nucleotide polymorphism identification in the caprine myostatin gene. J. Anim. Breed. Genet. 123:141-144. https://doi.org/10.1111/j.1439-0388.2006.00572.x
  12. Li, X. L., Z. L. Wu and Z. Z. Liu. 2006b. SNP identification and analysis in part of intron 2 of goat MSTN gene and variation within and among species. J. Hered. 97:285-289. https://doi.org/10.1093/jhered/esj026
  13. Liu, Z. Z., X. L. Li, Y. F. Gong, X. M. Jin and M. S. Feng. 2006. Relationship between polymorphism of goat MSTN gene intron 2 and body weight. Chinese J. Anim. Vet. Sci. 37:745-748.
  14. Marcq, F. 1998 Investigating the role of myostatin in the determinism of double muscling characterizing Belgian Texel sheep. Anim. Genet. 29(Suppl. 1):52.
  15. McPherron, A. C. and S. J. Lee. 1997. Double muscling in cattle due to mutations in the myostatin gene. Proc. Nat. Acad. Sci. US. 94:12357-123461.
  16. Mosher, D. S., P. Quignon, C. D. Bustamante, N. B. Sutter and C. S. Mellersh. 2007. A mutation in the myostatin gene increases muscle mass and enhances racing performance in heterozygote dogs. PLoS Genet. doi: 10.1371/journal.pgen.0030079.eor.
  17. Rieder, M. J., S. L. Taylor, A. G. Clark and D. A. Nickerson. 1999 Sequence variation in the human angiotensin converting enzyme. Nat. Genet. 22:59-62. https://doi.org/10.1038/8760
  18. Sall, J. P. 1990. Leverage plots for general linear hypothesis. Am. Stat. 44:303-315. https://doi.org/10.2307/2684356
  19. Scheet, P. and M. Stephens. 2006. A fast and flexible statistical model for large-scale population genotype data: Applications to inferring missing Genotypes and haplotypic phase. Am. J. Hum .Genet. 78:629-644. https://doi.org/10.1086/502802
  20. Shibata, M., K. Ohshima, T. Kojima, T. Muramoto, K. Matsumoto, M. Komatsu, K. Aikawa, S. Fujimura and M. Kadowaki. 2003. Nucleotide sequence of myostatin gene and its developmental expression in skeletal muscles of Japanese Black cattle. Anim. Sci. 74:383-390. https://doi.org/10.1046/j.1344-3941.2003.00130.x
  21. Stephens, M. and P. Donnelly. 2003. A comparison of bayesian methods for haplotype reconstruction from population genotype data. Am. J. Hum. Genet. 73:1162-1169. https://doi.org/10.1086/379378
  22. Stephens, M., N. J. Smith and P. Donnelly. 2001. A new statistical method for haplotype reconstruction from population data. Am. J. Hum. Genet. 68:978-989. https://doi.org/10.1086/319501
  23. Walling, G. A., P. M. Visscher, G. Simm and S. C. Bishop. 2001. Confirmed linkage for QTLs affecting muscling in Texel sheep on chromosomes 2 and 18. In: proceedings of the 52th Annual Meeting of the European Association for Animal Production. Budapest (Hungary), 26-29 August 2000.

Cited by

  1. miRNA-Mediated Relationships between Cis-SNP Genotypes and Transcript Intensities in Lymphocyte Cell Lines vol.7, pp.2, 2012, https://doi.org/10.1371/journal.pone.0031429
  2. Genetic variations in the myostatin gene (MSTN) in New Zealand sheep breeds vol.40, pp.11, 2013, https://doi.org/10.1007/s11033-013-2752-7
  3. ) Gene in Indian Goat Breeds vol.25, pp.1, 2014, https://doi.org/10.1080/10495398.2013.821994
  4. Polymorphism in the coding region sequence of GDF8 Gene in Indian Sheep vol.51, pp.11, 2015, https://doi.org/10.1134/S1022795415110149
  5. Effects of Castration on Androgen Receptor, IGF-I Ea, MGF and Myostatin Gene Expression in Skeletal Muscles of Male Pigs vol.22, pp.8, 2008, https://doi.org/10.5713/ajas.2009.80632
  6. SNPs in minimal promoter of myostatin (GDF-8) gene and its association with body weight in broiler chicken vol.42, pp.3, 2008, https://doi.org/10.1080/09712119.2013.846859
  7. Novel report on mutation in exon 3 of myostatin (MSTN) gene in Nilagiri sheep: an endangered breed of South India vol.51, pp.7, 2008, https://doi.org/10.1007/s11250-019-01873-7
  8. Effects of Intronic SNPs in the Myostatin Gene on Growth and Carcass Traits in Colored Polish Merino Sheep vol.11, pp.1, 2008, https://doi.org/10.3390/genes11010002
  9. Genetic variations in the Myostatin gene affecting growth traits in sheep vol.14, pp.2, 2008, https://doi.org/10.14202/vetworld.2021.475-482
  10. Key Genes Regulating Skeletal Muscle Development and Growth in Farm Animals vol.11, pp.3, 2021, https://doi.org/10.3390/ani11030835