Browse > Article
http://dx.doi.org/10.13045/jar.2019.00199

The Protective Effects of Zanthoxylum bungeanum Maxim Pharmacopuncture on Disuse Muscle Atrophy in Rat Gastrocnemius Muscle  

Chung, Yeon Joong (Department of Acupuncture & Moxibustion Medicine, College of Korean medicine, Daegu Haany University)
Lee, Yun Kyu (Department of Acupuncture & Moxibustion Medicine, College of Korean medicine, Daegu Haany University)
Lee, Bong Hyo (Department of Acupuncture & Moxibustion Medicine, College of Korean medicine, Daegu Haany University)
Kim, Jae Soo (Department of Acupuncture & Moxibustion Medicine, College of Korean medicine, Daegu Haany University)
Lee, Hyun-Jong (Department of Acupuncture & Moxibustion Medicine, College of Korean medicine, Daegu Haany University)
Publication Information
Journal of Acupuncture Research / v.36, no.4, 2019 , pp. 238-244 More about this Journal
Abstract
Background: This study aimed to investigate the protective effects of Zanthoxylum bungeanum Maxim pharmacopuncture on disuse muscle atrophy in the gastrocnemius muscle of rats. Methods: Thirty male 250 g Sprague-Dawley rats were distributed randomly into 3 groups. The left hindlimb immobilization was performed with casting tape for 2 weeks, and no treatment was given to the right hindlimb. Rats received pharmacopuncture and were injected daily on the BL57 with either 2 mL of Zanthoxylum bungeanum Maxim aqueous extract (ZM-W group), 1 mL pharmacopuncture of Zanthoxylum bungeanum Maxim ethanol extract (ZM-E group), or 2 mL normal saline (control group). After 2 weeks of immobilization, the weight of the whole gastrocnemius muscle was measured, and the morphology of both the left and the right gastrocnemius muscles were assessed by Hematoxylin and Eosin staining. To investigate the immobilization-induced muscular apoptosis, the immunohistochemical analysis of BAX and Bcl-2 was carried out. Results: ZM-W and ZM-E significantly inhibited the reduction in weight of the left gastrocnemius muscle, the reduction in the left myofibrils, and the cross-sectional area of gastrocnemius, as compared with the control. Moreover, the ZM-W and ZM-E groups showed significantly reduced immunoreactivity for BAX, and increased immunoreactivity of Bcl-2 in left gastrocnemius muscle compared with the control group. Conclusion: These results suggest that Zanthoxylum bungeanum Maxim pharmacopuncture has protective effects against immobilization-induced muscle atrophy by regulating the activity of apoptosis-associated BAX / Bcl-2 proteins in the gastrocnemius muscle.
Keywords
disuse muscle atrophy; Zanthoxylum bungeanum maxim; pharmacopuncture;
Citations & Related Records
Times Cited By KSCI : 6  (Citation Analysis)
연도 인용수 순위
1 Hoek JB, Pastorino JG. Ethanol, oxidative stress, and cytokine-induced liver cell injury. Alcohol 2002;27:63-68.   DOI
2 Dupont-Versteegden EE. Apoptosis in muscle atrophy: Relevance to sarcopenia. Exp Gerontol 2005;40:473-481.   DOI
3 Yu J, Zhou X, He X, Dai M, Zhang Q. Curcumin induces apoptosis involving bax/bcl-2 in human hepatoma SMMC-7721 cells. Asian Pac J Cancer Prev 2011;12:1925-1929.
4 Kim BH, Lee TS. Effect of adjuvant administration on gastrocnemius atrophy induced by ankle joint fixation in rats. J Korean Soc Integr Med 2017;5:49-55. [in Korean].
5 Kim BH. The effects of Daeyeoung-jeon on the prevention of disuse muscle atrophy in rats. Herb Formula Sci 2017;25:499-508. [in Korean].   DOI
6 Kim BH. The protective effects of Dangguibohyul-tang (Dangguibuxuetang) against disuse muscle atrophy in rats. J Korean Med Rehabil 2017;27:1-9. [in Korean].
7 Gong HM, Lee YK, Lee BH, Kim JS, Lee HJ. The effects of dokhwalgisaengtang against disuse muscle atrophy in gastrocnemius of rats. J Acupunct Res 2018;35:207-213.   DOI
8 Alway SE, Bennett BT, Wilson JC, Sperringer J, Mohamed JS, Edens NK et al. Green tea extract attenuates muscle loss and improves muscle function during disuse, but fails to improve muscle recovery following unloading in aged rats. J Appl Physiol 2015;118:319-330.   DOI
9 Powers SK, Smuder AJ, Judge AR. Oxidative stress and disuse muscle atrophy: Cause or consequence? Curr Opin Clin Nutr Metab Care 2012;15:240-245.   DOI
10 Wall BT, Dirks ML, van Loon LJ. Skeletal muscle atrophy during short-term disuse: implications for age-related sarcopenia. Ageing Res Rev 2013;12:898-906.   DOI
11 Powers SK, Wiggs MP, Duarte JA, Zergeroglu AM, Demirel HA. Mitochondrial signaling contributes to disuse muscle atrophy. Am J Physiol Endocrinol Metab 2012;303:31-39.   DOI
12 Calvani R, Joseph AM, Adhihetty PJ, Miccheli A, Bossola M, Leeuwenburgh C et al. Mitochondrial pathways in sarcopenia of aging and disuse muscle atrophy. Biol Chem 2013;394:393-414.   DOI
13 Marzetti E, Hwang JC, Lees HA, Wohlgemuth SE, Dupont-Versteegden EE, Carter CS et al. Mitochondrial death effectors: Relevance to sarcopenia and disuse muscle atrophy. Biochim Biophys Acta 2010;1800:235-244.   DOI
14 Kim BH. The protective effects of pueraria radix against chronic alcoholinduced muscle atrophy in rats. J Korean Med Rehabil 2017;27:1-8. [in Korean].
15 Powers SK. Can antioxidants protect against disuse muscle atrophy? Sports Med 2014;44:155-165.   DOI
16 Lee JW. Volatile flavor components of korean sancho fruit and tree (Zanthoxylum schinifolium). J Korean Soc Food Sci Nutr 1998;11:493-498. [in Korean].
17 Park JH, Park SS, Kim JM. Pharmacognostical Studies on the 'Cho Pi Na Mu'. Korean J Pharmacogn 2002:33:75-80. [in Korean].
18 Ahn MS, Won JS, Kim HJ, Han MN. A study on the antioxidative and antimicrobial activities of the Chopi (Zanthoxylum Pipperitum DC.) solvent extracts. Korean J Food Culture 2004;19:170-176. [in Korean].
19 Chung JW [Dissertation]. Methanolic extract of pericarpium zanthoxyli inhibits iNOS/ COX-2 and lipid peroxidation. Seoul (Korea): KyungHee University; 2013.
20 Jang MJ, Rhee SJ, Cho SH, Woo MH, Choi JH. A study on the antioxidative, anti-inflammatory and anti-thrombogenic effects of zanthoxylum piperitum DC extract. J Korean Soc Food Sci Nutr 2006;35:21-27. [in Korean].   DOI
21 Lee SG, Park CI. A study on the anti-microbial effect on S. mutans and antioxidant effect of zanthoxylum pericarpium extract. Korean J Herbology 2011;26:181-185. [in Korean].
22 Magne H, Savary-Auzeloux I, Remond D, Dardevet D. Nutritional strategies to counteract muscle atrophy caused by disuse and to improve recovery. Nutr Res Rev 2013;26:149-165.   DOI
23 Desaphy JF, Pierno S, Liantonio A, Giannuzzi V, Digennaro C, Dinardo MM et al. Antioxidant treatment of hindlimb-unloaded mouse counteracts fiber type transition but not atrophy of disused muscles. Pharmacol Res 2010;61:553-563.   DOI
24 Kwon OS [Dissertation]. A Study for establish standard of selecting points by measuring each parts of humam body. Iksan(Korea): WonKwang University; 2009. [in Korean].
25 Mulder FR, Gerrits KH, Kleine BU, Rittweger J, Felsenberg D, de Haan A et al. Stegeman, High-density surface EMG study on the time course of central nervous and peripheral neuromuscular changes during 8 weeks of bed rest with or without resistive vibration exercise, J Electromyogr Kinesiol 2009;19:208-218.   DOI
26 Wall BT, van Loon LJ. Nutritional strategies to attenuate muscle disuse atrophy. Nutr Rev 2013;71:195-208.   DOI
27 Desaphy JF, Pierno S, Liantonio A, De Luca A, Didonna MP, Frigeri A et al. Recovery of the soleus muscle after short- and long-term disuse induced by hindlimb unloading: effects on the electrical properties and myosin heavy chain profile. Neurobiol Dis 2005;18:356-365.   DOI
28 Matsumoto Y, Nakano J, Oga S, Kataoka H, Honda Y, Sakamoto J et al. The non-thermal effects of pulsed ultrasound irradiation on the development of disuse muscle atrophy in rat gastrocnemius muscle. Ultrasound Med Biol 2014;40:1578-1586.   DOI
29 Roberts D, Smith DJ. Biochemical aspects of peripheral muscle fatigue. Sports Med 1989;7:125-138.   DOI
30 Kano Y, Shimegi S, Takahashi H, Masuda K, Katsuta S. Changes in capillary luminal diameter in rat soleus muscle after hind-limb suspension. Acta Physiol Scand 2000;169:271-276.   DOI
31 Dirks ML, Wall BT, Snijders T, Ottenbros CL, Verdijk LB, Loon L. Neuromuscular electrical stimulation prevents muscle disuse atrophy during leg immobilization in humans. Acta Physiologica 2014;210:628-641.   DOI
32 Chen G, Mou C, Yang Y, Wang S, Zhao Z. Exercise training has beneficial anti-atrophy effects by inhibiting oxidative stress-induced MuRF1 upregulation in rats with diabetes. Life Sci 2011;89:44-49.   DOI
33 Udaka J, Terui T, Ohtsuki I, Marumo K, Ishiwata S, Kurihara S et al. Depressed contractile performance and reduced fatigue resistance in single skinned fibers of soleus muscle after long-term disuse in rats. J Appl Physiol 2001;111:1080-1087.   DOI
34 Midrio M. The denervated muscle: facts and hypotheses. A historical review. Eur J Appl Physiol 2006;98:1-21.   DOI
35 Yen Y, Tu P, Chen C, Lin Y, Hsieh S, Chen C. Role of acid-sensing ion channel 3 in sub-acute-phase inflammation. Molecular Pain 2009;5:1.   DOI
36 Fujita R, Tanaka Y, Saihara Y, Yamakita M, Ando D, Koyama K. Effect of molecular hydrogen saturated alkaline electrolyzed water on disuse muscle atrophy in gastrocnemius muscle. J Physiol Anthropol 2011;30:195-201.   DOI
37 Lee CJ, kim MS, Shen JY, Kim YD, Shin GH. The extraction condition of pungent compounds from Zanthoxylum piperitum D.C pericarps by using supercritical fluid extraction. Korean J Medicinal Crop Sci 2003;11:19-23. [in Korean].
38 Kim J, Cho YS, Seo KI, Joo OS, Shim KH. Antimicrobial activities of zanthoxylum shinifolium and zanthoxylum piperitum leaves. Korean J Postharvest Sci Technol 2000;7:195-200. [in Korean].
39 Shibaguchi T, Yamaguchi Y, Miyaji N, Yoshihara T, Naito H, Goto K et al. Astaxanthin intake attenuates muscle atrophy caused by immobilization in rats. Physiol Rep 2016;4:1-8.