Journal of the Korean Academy of Clinical Electrophysiology
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v.5
no.2
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pp.35-45
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2007
The purpose of this study was to examine spiral way movement of a trunk exerts on the movement ability. The details established to achieve for this article. This examination confirmed the weight, weight/height2 index, ratio of lumbar to pelvic, musculoskeletal quantity, push up for 2 minute, pitch a ball and voluntary isometric contraction with flexion and extension of knee joint of the subjects with spiral direct movement. Healthy eighteen subjects who understand fully the significance of procedure, consented to a plan, without neuromuscular disease were participated in two groups of experiment. The group were a spiral movement(9), rectilinear movement(9). Trunk movement tested 2 sessions of a spiral movement and rectilinear movement with a push up for 2 minute, 5days per a week, for the 4 weeks. This experiment tested 3 times with a sufficient rest for fatigue limitation. An analysis of the results used a paired samples t-test for difference from before and after experiment. The following results were obtained; At an internal change of the body, the musculoskeletal quantity was increased significantly to spiral movement group, but the weight was increased significantly, the musculoskeletal quantity was not significant to rectilinear movement. The movement ability evaluation for a external change was increased significantly in a push up for 2 minute, pitch a ball, isometric contraction with extension of knee joint of a spiral movement group, but a push up for 2 minute was increased significantly in a push up for 2 minute on the abdominal muscle training of a rectilinear movement group. As compared with a rectilinear movement, a spiral movement was more effect by cooperation with nerve and musculoskeletal system and an increase in movement ability was caused by learning acknowledgment, muscular reeducation. These results lead us to the conclusion that a spiral movement of trunk was more effect than a rectilinear movement, the coordination of nerve and musculoskeletal system was of importance of Multi-direction movement. Therefore, A further studies concerning the therapeutic exercise intervention and active-dynamic analysis could enhance the development of the most effect on the trunk.
A comparative study on the quality of meat from three breeds of goats reared in Bulgaria: Local Aboriginal (LA); Bulgarian White Dairy (BWD) and crossbreeds of local aboriginal with Saanen goats (LA ${\times}$ S). Eight intact young male goats from each breed have been included in the experiment. The animals from the three breeds have been reared under similar conditions. The animals have been slaughtered at the age of 2 months (at weaning) and at the age of 6 months (upon reaching sexual maturity). The following tests have been performed on samples of m. longissimus dorsi between $5^{th}$ and $6^{th}$ ribs: pH 45 min post mortum, colorimetrically color of meat, water holding capacity and fatty acid composition of the fat. The thickness of muscle fibre and the proportion of muscle, connective and adipose tissue have been microscopically determined at m. longissimus dorsi, m. semitendinosus and m. iliopsoas. After deboning and separating the bigger tendons, the chemical composition of the meat from the left half of the carcass has been determined. The results reveal lack of statistically significant differences among the three breeds concerning pH, the water holding capacity, color of meat, thickness of the muscle fibre and fatty acid composition of the fat. The carcass meat of crossbreeds of LA ${\times}$ S contains significantly more fat than the other two breeds. This influences the proportion of muscle, connective and adipose tissue in m. longissimus dorsi, m. semitendinosus and m. iliopsoas, resulting in more adipose tissue in the LA ${\times}$ S compared to young goats of the LA or the BWD. There are differences in slaughtering 2-month-old goats (at weaning) and 6-month-old ones (upon reaching sexual maturity). At a greater age the content of fat in the carcass increases, as well as the quantity of the adipose tissue, the intensity of the colour of the meat and there is a tendency towards thickening of the muscle fibre and increasing the water holding capacity of meat. The moisture content in meat decreases due to the increase of fat.
The development of the alpha2 isoform of (Na,K)ATPase which is high affinity ouabain receptors was studied in the differentiating nonfusing muscle cell line BC3H-1. T he differentiation process of BC3H-1 cell line was confirmed by 2-dexy-D-[$^3$H] glucose uptake experiment and the quantity of the expression of ${\alpha}_2$ isoform was measured using a whole cell [$^3$H] ouabain-binding assay. Undifferentiated growing BC3H-1 cells, myoblasts, exhibited low levels of insulin-stimulated glucose uptake and [$^3$H] ouabain-binding sites. In contrast, differentiated BC3H-1 cells, myocytes, had a 5.6-fold increase in insulin-stimulated glucose uptake and 5-fold increase in [$^3$H] ouabain-binding sites. Scatchard analysis showed that myocytes developed more [$^3$H] ouabain-binding sites than myoblasts vath a dissociation constant (kd) of 6${\times}10^{-8}$M and capacity of 6.l${\times}10^{-5}$ sites/cell. Therefore. it seems that myoblasts express low levels of ${\alpha}_2$ subunit and probably the majority of ${\alpha}_1$ subunit, whereas myocytes express high levels of ${\alpha}_2$ isoform. The results indicate that the expression of ${\alpha}_2$ isoform is developmentally regulated during differentiation and that BC3H-1 culture system provides an excellent model for the study of differentiation and mechanism of (Na,K)ATPase action in muscle which requires electrical excitability.
Ryu, Jeong Yeop;Park, Tae Hyun;Lee, Joon Seok;Oh, Eun Jung;Kim, Hyun Mi;Lee, Seok-Jong;Lee, Jongmin;Lee, Sang Yub;Huh, Seung;Kim, Ji Yoon;Im, Saewon;Chung, Ho Yun
Archives of Plastic Surgery
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v.49
no.1
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pp.115-120
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2022
Background In addition to vascular endothelial cells, vascular smooth muscle cells (VSMCs) are subject to continuous shear stress because of blood circulation. The angiogenic properties of VSMCs in extracranial arteriovenous malformations (AVMs) may exceed those of normal blood vessels if the body responds more sensitively to mechanical stimuli. This study was performed to investigate the hypothesis that rapid angiogenesis may be achieved by mechanical shear stress. Methods VSMCs were obtained from six patients who had AVMs and six normal controls. The target genes were set to angiopoietin-2 (AGP2), aquaporin-1 (AQP1), and transforming growth factor-beta receptor 1 (TGFBR1). Reverse-transcriptase polymerase chain reaction (RT-PCR) and real-time PCR were implemented to identify the expression levels for target genes. Immunofluorescence was also conducted. Results Under the shear stress condition, mean relative quantity values of AGP2, AQP1, and TGFBR1 in AVM tissues were 1.927±0.528, 1.291±0.031, and 2.284±1.461 when compared with neutral conditions. The expression levels of all three genes in AVMs were higher than those in normal tissue except for AQP1 under shear stress conditions. Immunofluorescence also revealed increased staining of shear stress-induced genes in the normal tissue and in AVM tissue. Conclusions Shear stress made the VSMCs of AVMs more sensitive. Although the pathogenesis of AVMs remains unclear, our study showed that biomechanical stimulation imposed by shear stress may aggravate angiogenesis in AVMs.
Two concurrent signals of the pulse wave measured from both hands' radial artery in un-pressurization condition using the prototype model of two clip-type pulsimeters with a permanent magnet and Hall device are investigated. The phase differences of two pulse waves from 22 subjects have some distinct points according to the handedness. Thus, the propagation of the pulse wave calculated from phase difference is both fast and slow to each other. It is confirmed that this phenomenon comes from the difference of blood vessel hardness between right- and left-hand of each subject rather than a quantity of muscle.
Genetic modification enables modification of target genes or genome structure in livestock and experimental animals. These technologies have not only advanced bioscience but also improved agricultural productivity. To introduce a foreign transgene, the piggyBac transposon element/transposase system could be used for production of transgenic animals and specific target protein-expressing animal cells. In addition, the clustered regularly interspaced short palindromic repeat-CRISPR associated protein 9 (CRISPR-Cas9) system have been utilized to generate chickens with knockout of G0/G1 switch gene 2 (G0S2) and myostatin, which are related to lipid deposition and muscle growth, respectively. These experimental chickens could be the invaluable genetic resources to investigate the regulatory pathways and mechanisms of improvement of economic traits such as fat quantity and growth. The gene-edited animals could also be applicable to the livestock industry.
The main purpose of this study is to identify the effects of a structured nursing intervention program on dysmenorrhea in middle school girls. This study employed two methods of research at the same time: a quasi-experiment pre- and post-test to use for comparison. Subjects were middle school girls assigned to the research program: 40 girls in the second grade, 40 girls in the third grade at K Girls' Middle School and H Girls' Middle School located in J city. The data were collected for 68 days from August 23 to October 30, 1999. The data were collected by observation technique and by a self-administered questionnaire. The instruments used for this study were as follows: the "Face Interval Pain Scale" menstrual pain instrument developed by McGreth(1987) and the "Menstrual Distress Questionnaire" dysmenorrhea instrument developed by Moos(1968). The dysmenorrhea knowledge instrument was developed by the researcher and refered to above. The research procedure was as follows: after preliminary examination, the experimental groups were provided educational programs with O.H.P. films developed by the researcher and with the videotape "first meeting" supported by P&G company and produced by EBS. The groups followed the educational programs twice for two weeks. At the beginning of menstruation, these subjects could choose one among the following choices: * Jacobson's progressively muscle relaxation method and massage treatment * Jacobson's progressively muscle relaxation method and hot bag treatment * Jacobson's progressively muscle relaxation method, a pain killer treatment, and massage treatment * Jacobson's progressively muscle relaxation method, a pain killer treatment, and hot bag treatment The dysmenorrhea of the control group were measured during the same period. The analysis of the collected data was done using an SPSS-PC+, descriptive statistic including real numbers, percentage, averages, standard deviations and t-test, $x^2$ test, and ANCOVA. The results of this study were as follows. (1) The knowledge level of the girls provided with the planned nursing intervention program was higher when compared to these who did not follow the educational programs. (2) The original difference in the dysmenorrhea level having been controled, the dysmenorrhea level made, nevertheless, a statistical difference between the experimental group and the control group(F=63.8, p=.00). Observed pain on quantity measurement of dysmenorrhea showed significant difference depending on the treatment(t=4.6, p=.00). In conclusion, those in the planned nursing intervention program had a higher knowledge level and fewer symptoms than the control group. Accordingly, the nursing intervention program for dysmenorrhea developed by the researcher can be effectively applied in nursing practice and can be the foundation for this kind of program.
There is considerable interests in the effect of regular, vigorous exercise, in particular weight training as a possible measure in improving myocardial function. The present investigation aimed to examine possible effect of a long-term weight training program on the heart in aging rats. Male rats aged 3-,10-, and 20-month-old were divided at random into a control (sedentary) and the exercise group. The training group was exercised for 5 days a week by 1 RM of ten times with weight training apparatus. This investigation examined the changes of the heart muscle relative to histological, ultrastructural, cytochemical, and stereological studies in rats. Quantity of lipofuscin pigments was clearly increased in the weight training group of 15-month-old rats, and mitochondrial degeneration, vacuolization, and interstitial proliferation were observed as well. In the weight training group of 25-month-old rats the frequency as separated intercalated discs, fraying myofibrils and hypercontraction band increased in number compared with the same 15-mon1h-old group. From the experimental result of glucose-6-phosphatase activity, the enzyme activities decreased in the weight training group of 15-month-old rats, and more decreased in the same 25-month-old group. In stereological study, both 15- and 25-month-old training groups, mitochondrial and myofibrilar volume densities significantly decreased, whereas interstitial volume density significantly increased. From the experimental results obtained in the present study, it is suggested that long-term weight training exercise do not cause any significantly qualitative and quantitative ultrastructural change of the heart muscle in the young. On the contrary, long-term weight training exercise stress may actually induce degenerative changes in the heart muscle in the old age.
Recently, young men have become more muscular as they become more interested in physical figure. However, most of these muscular men have fit problems regarding ready-made clothes. In view of this, this study aimed to develop a prototype jacket pattern for muscular men. For this study, five muscular men were selected to put on existing jacket pattern for wearing tests. The regression formula, in which muscular men body measures were adopted, was applied to unsuitable parts, especially the areas determined not to be appropriate in the evaluation of existing jacket pattern wearing tests. After the first and the second jacket pattern wearing tests, the final jacket pattern suitable for muscular men was developed. The results of the study were as follows: In order to make up for the problem of the loosening of the lapel area, due to the development of the chest muscle, the chest circumference line on the chest area of the pattern was cut to be 1.0cm wide; thus, the front length was modified with an increase. The wearing tests found that a wearers felt discomfort from the tight armhole area, so the armhole depth was set to be a little lower than that of ready-made clothes. A muscular men needs much more extra quantity in this area because the upper part of the back side is projected due to the greater development of the trapezius muscle and the deltoid than in average men. Hence, concerning the standard line for the length of the back interscye, ease of 1.0cm was added to the regression equation formula {(0.371${\times}$chest circumference+3.145)/2} in order to resolve the discomfort with the back area. Also, for the biacromion length, the upper arm protruded more than the shoulder point of the jacket because of the development of the deltoid and the upper arm muscle, and it was set to be wider than the actual shoulder. In order to solve the problem of discomfort from the narrow neck area during the wearing of a jacket owing to the development of the trapezius muscle, extra ease of 0.5cm was added to chest circumference/12-0.5cm in the existing jacket prototype to the width of back of the neck, and it was corrected to be chest circumference/12.
The male and female muscle of the 16 major bands in the protein patterns of the tissues of the mud-snail, Cipangopaludina chinensis malleata, were almost similar, and the bands E (Mr. 41,500), H (Mr. 52,100), L (Mr. 71,700), N(Mr. 98,500), O (Mr. 107,900) and P (Mr. 112,900) had the same molecular weights which appear in the muscles, testis and ovary. But it showed that the testis and the ovary had their characteristic patterns, and it was also observed that, besides the bands common to all tissues, the bands which appear both in the testis and the ovary are about five: the bands d (Mr. 15,600), k (Mr. 37,100), p (Mr. 57,000), s (Mr. 80,300), and v (Mr. 105,400). The result of the amino acid analysis showed that the quantity and composition of amino acid appeared, as the protein pattersn did, in the order of ovary, muscles and testis. This reveals that in the ovary a great quantity of protein which appears in the whole body was investigated, while the testis contained only little protein due to the phenomenon of morphogenesis in which sperms were developed through the condensation of the nuclei.
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