• Title/Summary/Keyword: Muscle Growth Index

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Insulin-Like Growth Factors-1 Receptor (IGF-1R) Expression and the Phosphorylation of Endogenous Substrates Lead to Maturation of the Pacific oyster, Crassostrea gigas

  • Park, Su-Jin;Choi, Youn Hee
    • Development and Reproduction
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    • v.25 no.1
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    • pp.67-72
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    • 2021
  • This study investigated the IGF-1 signal in specific tissues using Pacific oysters artificially matured via water temperature elevation. Pacific oysters were subjected to water temperature elevation from March to June, and 20 were randomly sampled each month. The condition index (CI) and tissue weight rate (TWR) were examined by measuring shell length, shell height, shell width, and soft tissue weight. The IGF-1 signal in tissues (adductor muscle, digestive glands, gills, labial palps, mantle edges, and gonads) was analyzed by sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting. From April to June, the TWR of females and males increased from 19.1±2.9 to 21.0±3.6 and 18.2±2.0 to 19.2±2.5, respectively, while the CI remained the same. The IGF-1 signal in each tissue differed. IGF-1 was expressed in the adductor muscle, while tyrosine was expressed in all tissues. The phosphor (p)-ERK and p-AKT activities were high in the adductor muscle, mantle edge, and gonads. IGF-1 signaling affected the growth and maturity of the Pacific oysters examined.

The Effect of Resistance Training Intensity on Muscle Damaged Index and Aging-Related Hormones in Obese Middle-Aged Women (저항트레이닝 운동 강도가 비만 중년 여성의 근 손상지표와 노화관련 호르몬에 미치는 영향)

  • SHIN, So-Young;JANG, Byung-Ho;SHIN, Koun-Soo
    • Journal of Fisheries and Marine Sciences Education
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    • v.27 no.4
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    • pp.924-937
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    • 2015
  • The purpose of this study was to identify the effect of resistance training intensity levels on muscle damaged index and aging-related hormones in obese middle-aged women. Thirty middle-aged women having over 30% of body fat participated in this study. The subjects were assigned into 3 groups on the basis of workout intensity, 50% intensity, 60% intensity, and 70% intensity. After performing three different types of exercise for 12 weeks, the levels of creatine kinase (CK), lactate dehydrogenase (LDH), melatonin, dehydroepiandrosterone-sulphate (DHEA-S), and growth hormone (GH) in all subjects were assessed before and after the program. Muscle damaged index was effectively changed in low exercise intensity group. However, aging-related hormones were effectively changed in high exercise intensity group. DHEA-S and GH were significantly increased in the 70% intensity group than the other groups.

Relationship between Condition Index Values and Expression Levels of Gene and Protein in the Adductor Muscle of Diploid and Triploid Oysters Crassostrea gigas

  • Su-Jin Park;Youn Hee Choi
    • Development and Reproduction
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    • v.26 no.4
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    • pp.165-174
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    • 2022
  • Three proteins [myosin heavy chain (MHC), filamin-C fragment (FIL-C), and actin 2 (ACT2)] were identified in adductor muscle from diploid and triploid Pacific oysters (Crassostrea gigas) and the relationship between the condition index (CI) and mRNA expression of these genes was investigated, together with the mRNA expression of molluscan insulin-related peptide (MIP), C. gigas insulin receptor-related receptor (CIR), and insulin-like growth factor binding protein complex acid labile subunit (IGFBP-ALS). Monthly changes in the CI were similar to the changes in the tissue weight rate in both groups. ACT2 and MHC mRNA expression was statistically higher in the triploid than the diploid, while FIL-C mRNA expression was significantly higher in the diploid (p<0.05). The MIP, CIR, and IGFBP-ALS mRNA expression of the diploid oysters were all significantly higher in July than in other months (p<0.05). The MIP, CIR, and IGFBP-ALS mRNA expression in the triploid oysters was high in July, but there were no significant differences (p>0.05). Changes in the expression levels of the genes investigated in this study could be used as intrinsic indicators of the annual growth, maturity, and spawning period of cultured diploid and triploid C. gigas in Tongyeong, Korea.

A study of Association between Growth and Body Composition in Teenagers (청소년의 성장과 체성분과의 상관성에 관한 연구)

  • 홍상훈;김성환;강창완
    • The Journal of Korean Medicine
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    • v.22 no.4
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    • pp.114-120
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    • 2001
  • Objectives : Man adapts himself to nature, so accordingly as he grows up, changes of body composition appear. This study measured the changes of body compositions on the ground of Sasang constitution and seasons Methods : 253 middle school students who live in Busan were studied from the 20th of March, 1999 to the 20th of March, 2001. We measured height and body compositions with Inbody 3.0, six times during that period. Results : According as they grew up, total body water, muscle area, body weight, BMI (Body Mass Index), BMR (Basal Metabolic Rate), ACM (Arm Muscle Circumference), and BCM (Body Cell Mass) increased, but fat body weight ratio decreased. From March to June, total body water, muscle area, height, ACM, and BCM increased. From September to December fat body weight, fat body weight ratio, waist hip ratio increased as compared with other seasons. Height of Soyangin and BMI of both Soyangin and Taeumin increased more than those of other groups, with statistical significance Conclusions : The growth of man is adapting oneself to changes of nature, and it corresponds to the theory of (correspondence between man and universe).

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Effect of Dietary Conjugated Linoleic Acid on Growth, Lipid Class, and Fatty Acid Composition in Rainbow Trout(Oncorhynchus mykiss)

  • Guo, Rui;Jeong, U-Cheol;Kang, Seok-Joong;Choi, Yeung-Jun;Choi, Byeong-Dae
    • Fisheries and Aquatic Sciences
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    • v.11 no.3
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    • pp.125-132
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    • 2008
  • The development of a fish that functionally provides both antioxidant and fat-reducing effects is an important goal in nutrition and aquaculture research. Dietary conjugated linoleic acid(CLA) can be successfully incorporated in fish muscle and viscera, but CLA and carotenoids have not been evaluated in such fish. An 8 weeks growth trial was conducted using rainbow trout, and all fish were fed twice daily with experimental diets containing graded amounts of CLA(1% and 5%). At the end of the experiment, the daily growth index, feed conversion rate, lipid class, and fatty acid composition were determined. Dietary CLA did not enhance the growth parameters of rainbow trout but did improve the feed conversion rate. The muscular polar lipid content decreased during the feeding period, while the content was stable in the viscera. In addition, a diet high in CLA decreased the polyunsaturated fatty acid content, but had no effect on the content of monounsaturated and saturated fatty acid in muscle.

Effects of Dietary n-3 Highly Unsaturated Fatty Acids and Vitamin E Levels on the Growth and Fatty Acid Composition of Rockfish Sebastes schlegeli

  • Lee, Sang-Min
    • Fisheries and Aquatic Sciences
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    • v.13 no.2
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    • pp.118-126
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    • 2010
  • A feeding trial was conducted to investigate the effects of different levels of dietary n-3 highly unsaturated fatty acids (HUFA) (1.1-5.6%) and vitamin E (70 and 400 mg/kg) on the growth and body composition of juvenile rockfish. Six isonitrogenous (45% crude protein) and isolipidic (17% crude lipid) diets were formulated to contain graded levels of n-3 HUFA and vitamin E. Diets 1, 2 and 3 consist of 400 mg vitamin E/kg diet with graded levels of 1.1, 3.0, and 5.6% n-3 HUFA, respectively. Graded levels of n-3 HUFA (1.1, 3.0, and 4.0%) were added in diets 4, 5 and 6, respectively, containing 70 mg vitamin E/kg diet each. At the end of feeding trial, growth performance of rockfish was affected by neither dietary n-3 HUFA nor vitamin E levels. Feed efficiency and hepatosomatic index were slightly decreased (P<0.05) with increment of dietary n-3 HUFA at each dietary vitamin E level. Dietary vitamin E and n-3 HUFA levels did not affect proximate composition and vitamin E concentration in the dorsal muscle of rockfish. Liver moisture and crude protein contents positively related to dietary n-3 HUFA levels. Liver lipid content and hematocrit value were significantly decreased (P<0.05) by increasing dietary n-3 HUFA levels. Eicosapentaenoic acid (20:5n-3; EPA) and docosahexaenoic acid (22:6n-3; DHA) concentrations in the dorsal muscle significantly correlated to dietary n-3 HUFA levels, except for fish fed the diet 6 containing 4% n-3 HUFA and 70 mg vitamin E/kg diet. EPA concentration in the dorsal muscle of fish fed the diet 6 was significantly lower than that of fish fed the diets 2, 3 and 5. The present findings suggest that feeding of diets containing excessive n-3 HUFA level with varying addition of vitamin E may alter fatty acid composition in the dorsal muscle, but do not affect growth of juvenile rockfish.

Effect of Supplementing 2-Hydroxy-4-(Methylthio) Butanoic Acid and DL-methionine in Corn-soybean-cottonseed Meal Diets on Growth Performance and Carcass Quality of Broilers

  • Liu, Y.L.;Song, G.L.;Yi, G.F.;Hou, Y.Q.;Huang, J.W.;Vazquez-Anon, M.;Knight, C.D.
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.8
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    • pp.1197-1205
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    • 2006
  • This experiment was conducted to compare the effects of feeding DL-2-hydroxy-4-(methylthio)butanoic acid (HMTBA) and DL-methionine (DLM) supplemented corn-soybean-cottonseed meal diets on growth performance, carcass composition, and muscle color of broilers. The trial was designed as a $2{\times}3{\times}2$ factorial experiment, including two methionine (Met) sources (HMTBA and DLM), three equimolar graded levels of Met supplementation (i.e., 0.08, 0.16, and 0.24% in the starter diet and 0.07, 0.14, and 0.21% in the grower and finisher diets, respectively), and two sexes (male and female). Additionally, one basal diet for each sex was formulated to be limiting in Met to test the dosage response of increasing supplemental Met levels. Four hundred and twenty 10-d-old broilers were randomly allotted to 14 treatments (seven each for males and females), with five replicate pens per treatment and six chicks per pen. There was no difference (p>0.05) between the two Met sources in growth performance and muscle deposition of broilers throughout the whole experimental period (d 10 to 49). With the increasing Met supplementation levels, average daily gain was increased (quadratic; p<0.01) during the starter, grower, and overall phases, average daily feed intake was increased (quadratic; p<0.01) during the starter phase, and feed:gain ratio was decreased (quadratic; p<0.05) during the grower and overall phases. At the end of finisher phase, Met supplementation increased breast muscle content (quadratic; p<0.01) and thigh muscle content (linear; p<0.05), and decreased abdominal fat content (quadratic; p<0.02). Compared to the broiler fed DLM, broilers fed HMTBA had superior breast and thigh muscle coloration (p<0.01). Male broilers had higher weight gain and feed intake and better feed conversion than female broilers (p<0.01). The fat content of thigh muscle in female broilers was higher than that of male broilers (p<0.03). The best fit comparison of HMTBA vs. DLM was determined by Schwarz Bayesian Criteria index, which indicated that the average relative bioefficacy of HMTBA vs. DLM was 120% with 95% confidence limit 67 to 172%. These results indicated that Met supplementation improved growth performance and carcass quality of broilers fed corn-soybean-cottonseed meal diets irrespective of Met sources. Compared to DLM, HMTBA has the same molar bioefficacy on improving the growth performance and carcass quality of broilers; however, HMTBA fed birds had superior meat color to DLM fed birds.

Variation Characteristics of Vegetation Index(NDVI) Using AVHRR Images and Spectral Reflectance Characteristics (AVHRR영상과 분광반사특성을 이용한 식생지수(NDVI)의 변동특성)

  • Park, Jong-Hwa;Ryu, Kyong-Shik
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.8 no.2
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    • pp.33-40
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    • 2005
  • The objective of this research was to find an indirect method to estimate spectral reflectance and NDVI(Normalized Difference Vegetation Index) efficiently, using the spectroradiometer and NOAA AVHRR satellite data. For collecting RS base data, used spectro-radiometer that measures reflection characteristics between 300~1,100nm was used and measured the reflection of vegetation from paddy rice during the growing season at Chungbuk national university's farm in 2002. The feasibility of detecting the temporal variation in the spectral reflectance and NDVI in paddy rice were conducted on eight growth stages. AVHRR data were collected in eight different months over a one year period in 2002. The results were compared with those obtained by analyzing NDVI characteristics. The spectral reflectance and NDVI of paddy rice have a great effect on the growth condition. Considerably, NDVI was increased by developing muscle fiber tissue at the near infrared wavelength until the Booting stage. Then the NDVI increased until the Maturity stage and then decreased until harvest. The highest month was at July and the lower month was at March. The difference NDVI analysis using March and another months data was conducted, the results were provided information on the growth condition of crops.

Effects of Dietary Lipid Level on Growth and Body Composition of Juvenile Red Sea Bream (Pagurus major) during Winter (저수온기 참돔 치어의 성장 및 체조성에 미치는 사료내 지질의 영향)

  • Hwang, Hyung-Kyu;Kim, Kyoung-Duck;Park, Min-Woo;Han, Seock-Jung;Kang, Yong-Jin;Kim, Eung-Oh;Kim, Dae-Hyun
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.42 no.4
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    • pp.344-348
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    • 2009
  • This study was conducted to investigate effects of dietary lipid level on growth and body composition of juvenile red sea bream in low temperatures. Duplicate groups of fish (initial body weight of 79 g) were fed one of three isonitronic diets (47% crude protein) containing different lipid levels (10%, 17% and 22%) for 25 weeks during the winter season. Weight gain and survival of fish fed 17% lipid diet were significantly (P<0.05) higher than those fish fed the 10% or 22% lipid diet. Protein efficiency ratio, daily feed intake, condition factor, hepatosomatic index and viscerasomatic index were not affected by dietary lipid level, but feed efficiency of fish fed 10% lipid diet was significantly (P<0.05) lower than those fish fed the 17% or 22% lipid diet. Proximate composition of the whole body, liver, viscera and dorsal muscle were not significantly different among all groups except for crude protein content of dorsal muscle. The contents of 16:0, 18:0, 20:4n-3 and 20:5n-3 of the whole body were significantly (P<0.05) affected by dietary lipid level. The results of this study suggest that an increased dietary lipid level from 10% to 17% can improve growth of juvenile red sea bream in low temperature periods.

Fatty Acid Composition of Chub Mackerel (Scomber japonicus) Fed a Diet Fortified with CLA and Ascidian (Halocynthia roretzi) Tunic Extracts (멍게껍질 추출색소 및 CLA (Conjugated Linoleic Acid)가 함유된 사료를 섭취한 고등어 (Scober japonicus)의 일반성분 및 지방산 조성)

  • Park, Eun-Jung;Kim, Jong-Tae;Kang, Seok-Joong;Choi, Byeong-Dae
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.43 no.6
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    • pp.581-588
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    • 2010
  • To examine the functional properties of conjugated linoleic acid (CLA) and ascidian tunic extracts in fish, we compared mackerel fed ascidian tunic extract and CLA (CA25) and a control group. The daily growth index of CA25 was 1.92 compared to 1.86 in the control group. The viscerosomatic index of CA25 was 36.7% lower than that of the control group. After 8 weeks, the protein content decreased from 19.7 to 17.5% in the CA25 group. The ascidian tunic extract content in the viscera was much higher than in muscle (0.13 vs. 0.03 mg/100 g) after 8 weeks. At the start, the n-3 fatty acid content of the experimental fish was 25.2% in muscle and 23.7% in viscera. The CLA content in muscle in the CA25 group was 2.1% after 4 weeks and 2.3% after 8 weeks. By contrast, the CLA content in viscera did not change after 8 weeks.