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http://dx.doi.org/10.12717/DR.2021.25.1.67

Insulin-Like Growth Factors-1 Receptor (IGF-1R) Expression and the Phosphorylation of Endogenous Substrates Lead to Maturation of the Pacific oyster, Crassostrea gigas  

Park, Su-Jin (Dept. of Fisheries Biology, Pukyong National University)
Choi, Youn Hee (Dept. of Fisheries Biology, Pukyong National University)
Publication Information
Development and Reproduction / v.25, no.1, 2021 , pp. 67-72 More about this Journal
Abstract
This study investigated the IGF-1 signal in specific tissues using Pacific oysters artificially matured via water temperature elevation. Pacific oysters were subjected to water temperature elevation from March to June, and 20 were randomly sampled each month. The condition index (CI) and tissue weight rate (TWR) were examined by measuring shell length, shell height, shell width, and soft tissue weight. The IGF-1 signal in tissues (adductor muscle, digestive glands, gills, labial palps, mantle edges, and gonads) was analyzed by sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting. From April to June, the TWR of females and males increased from 19.1±2.9 to 21.0±3.6 and 18.2±2.0 to 19.2±2.5, respectively, while the CI remained the same. The IGF-1 signal in each tissue differed. IGF-1 was expressed in the adductor muscle, while tyrosine was expressed in all tissues. The phosphor (p)-ERK and p-AKT activities were high in the adductor muscle, mantle edge, and gonads. IGF-1 signaling affected the growth and maturity of the Pacific oysters examined.
Keywords
Pacific oyster; Condition index; Tissue weight rate; Insulin-like growth factors-1 (IGF-1) signal;
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1 Foncea R, Andersson M, Ketterman A, Blakesley V, Sapag-Hagar M, Sugden PH, LeRoith D, Lavandero S (1997) Insulin-like growth factor-I rapidly activates multiple signal transduction pathways in cultured rat cardiac myocytes. J Biol Chem 272:19115-19124.   DOI
2 Hwang IJ, Han JC, Hur YB, Lim HJ (2016) Seasonal variation in the body composition, amino acid, fatty acid and glycogen contents of triploid Pacific oyster, Crassostrea gigas in western coastal waters of Korea. Korean J Malacol 32:269-277.   DOI
3 Le Bail PY, Gentil V, Noel O, Gomez JM, Carre F, Le Goff P, Weil C (1998) Structure, function, and regulation of insulin-like growth factors in fish. Ann NY Acad Sci 839:157-161.   DOI
4 Liu Z, Han T, Fishman S, Butler J, Zimmermann T, Tremblay F, Harbison C, Agrawal N, Kopchick JJ, Schaffler MB, Yakar S (2017) Ablation of hepatic production of the acid labile subunit in the bovine-GH transgenic mice: Effects on organ and skeletal growth. Endocrinology 158:2556-2571.   DOI
5 Choi YH, Nam TJ (2014) Influence of the toxicity of cryoprotective agents on the involvement of insulin-like growth factor-I receptor in surf clam (Spisula sachalinensis) larvae. Cryo Letters 35:537-543.
6 Choi YH, Kim EY, Nam TJ (2018) Involvement of insulin-like growth factor in intraspecific variation in growth of Pacific oyster Crassostrea gigas during winter. Fish Sci 84:1017-1024.   DOI
7 Park MS, Lyu HY, Lee TS (1999) Investigation on the cause of bad natural seed collection of the Pacific oyster, Crassostrea gigas: Relationships between the conditions of mother shell and the viability of the released eggs and larvae based on the pathological and embryological survey. J Korean Fish Soc 32:62-67.
8 Kim MA, Shim KB, Par JS, Oh EG, Shi SB, Par K, Lim CW (2014) Seasonal variation in the proximate composition, pH and glycogen content of oysters Crassostrea gigas collected in Geoje and Jaran Bay in Korea. Korean J Fish Aquat Sci 47:713-718.   DOI
9 Anguis MV, Canavate JP (2005) Spawning of captive Senegal sole (Solea senegalensis) under a naturally fluctuating temperature regime. Aquaculture 243:133-145.   DOI
10 Choi YH, Chang YJ (2003) Gametogenic cycle of the transplanted-cultured pearl oyster, Pinctada fucata martensii (Bivalvia: Pteriidae) in Korea. Aquaculture 220:781-790.   DOI
11 Duan C (1997) The insulin-like growth factor system and its biological actions in fish. Am Zool 37:491-503.   DOI
12 Adams TE, Epa VC, Garrett TPJ, Ward CW (2000) Structure and function of the type 1 insulin-like growth factor receptor. Cell Mol Life Sci 57:1050-1093.   DOI
13 Kim KT, Lim HK, Chang YJ (2013) Survival rates with time course of frozen-thawed Pacific oyster larvae in indoor rearing system. Dev Reprod 17:337-343.   DOI
14 Lee JM, Park AJ, Cho SM, Park KD (2008) Growth comparison of the Pacific oyster, Crassostrea gigas, by three different suspended time around coast of Gyeongnam. Korean J Malacol 24:109-119.
15 Lim HJ, Lee TS, Cho PG, Back SH, Byun SG, Choi EH (2011) The production efficiency of cupped oyster Crassostrea gigas sprat according to clutch and growth comparing diploid and triploid oysters in off-bottom culture for tidal flat utilization. Korean J Fish Aquat Sci 44:259-266.   DOI
16 De Oliveira Ramos C, de Miranda Gomes CHA, Magalhaes ARM, dos Santos AI, de Melo CMR (2014) Maturation of the Mangrove Oyster Crassostrea gasar at different temperatures in the laboratory. J Shell Res 33:187-194.   DOI
17 Moriyama S, Ayson FG, Kawauchi H (2000) Growth regulation by insulin-like growth factor-I in fish. Biosci Biotechnol Biochem 64:1553-1562.   DOI
18 Loosanoff VL, Davis HC (1952) Temperature requirements for maturation of gonads of northern oysters. Biol Bull 103:80-96.   DOI