• 한국광학회:학술대회논문집
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• 한국광학회 2009년도 동계학술발표회 논문집
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• pp.297-298
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• 2009

파장 분할 방식의 광섬유 격자 센서의 멀티플렉싱 능력을 향상시키기 위하여 이중 격자의 파장 간격 변조로 구분되는 다중 광섬유 격자 센서 시스템을 제안하였으며, 서로 다른 파장 간격을 가지는 센서의 상태와 위치를 정확히 구별할 수 있는 방법을 연구하고 씨뮬레이션을 통하여 확인하였다. 또한 변형력 하에서 광섬유격자 센서들간의 스펙트럼 중첩으로 불명확한 브래그 피크를 최급 강하법(steepest descent method) 알고리즘을 사용하여 약 3 pm이내의 표준편차로 결정할 수 있었다.

• 분석과학
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• 제27권3호
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• pp.121-139
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• 2014

In this review, we will discuss aptamer technologies including in vitro selection, signal transduction mechanisms, and designing aptamers and aptazyme for label-free biosensors and catalysts. Dye-displacement, a typical label-less method, is described here which allows avoiding relatively complex labeling steps and extending this application to any aptamers without specific conformational changes, in a more simple, sensitive and cost effective way. We will also describe most recent and advanced technologies of signaling aptamer and aptazyme for the various analytical and clinical applications. Quantum dot biosensor (QDB) is explained in detail covering designing and adaptations for multiplexed protein detection. Application to aptamer array utilizing self-assembled signaling aptamer DNA tile and the novel methods that can directly select smart aptamer or aptazyme experimentally and computationally will also be finally discussed, respectively.

• Biotechnology and Bioprocess Engineering:BBE
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• 제11권5호
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• pp.455-461
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• 2006

In this study, we have described a method for the fabrication of a protein chip on silicon substrate using hydrophobic thin film and microfluidic channels, for the simultaneous detection of multiple targets in samples. The use of hydrophobic thin film provides for a physical, chemical, and biological barrier for protein patterning. The microfluidic channels create four protein patterned strips on the silicon surfaces with a high signal-to-noise ratio. The feasibility of the protein chips was determined in order to discriminate between each protein interaction in a mixture sample that included biotin, ovalbumin, hepatitis B antigen, and hepatitis C antigen. In the fabrication of the multiplexed assay system, the utilization of the hydrophobic thin film and the microfluidic networks constitutes a more convenient method for the development of biosensors or biochips. This technique may be applicable to the simultaneous evaluation of multiple protein-protein interactions.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2011년도 제40회 동계학술대회 초록집
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• pp.431-431
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• 2011

Graphene, two dimensional sheet of sp2-hybridized carbon, has attracted an enormous amount of interest due to excellent electrical, chemical and mechanical properties for the application of transparent conducting films, clean energy devices, field-effect transistors, optoelectronic devices and chemical sensors. Especially, graphene is promising candidate to detect the gas molecules and biomolecules due to the large specific surface area and signal-to-noise ratios. Despite of importance to the disease diagnosis, there are a few reports to demonstrate the graphene- and rGO-FET for biological sensors and the sensing mechanism are not fully understood. Here we describe scalable and facile fabrication of rGO-FET with the capability of label-free, ultrasensitive electrical detection of a cancer biomarker, prostate specific antigen/${\alpha}1$-antichymotrypsin (PSA-ACT) complex, in which the ultrathin rGO sensing channel was simply formed by a uniform self-assembly of two-dimensional rGO nanosheets on aminated pattern generated by inkjet printing. Sensing characteristics of rGO-FET immunosensor showed the highly precise, reliable, and linear shift in the Dirac point with the analyte concentration of PSA-ACT complex and extremely low detection limit as low as 1 fg/ml. We further analyzed the charge doping mechanism, which is the change in the charge carrier in the rGO channel varying by the concentration of biomolecules. Amenability of solution-based scalable fabrication and extremely high performance may enable rGO-FET device as a versatile multiplexed diagnostic biosensor for disease biomarkers.

• 천문학회지
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• 제29권spc1호
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• pp.389-390
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• 1996

The CCDs are widely used in astronomical observations either in direct imaging use or spectroscopic mode. However, the areas of available sensors are too small for large imaging format. One possibility to obtain large detection area is to assemble mosaics of CCD, and drive them simultaneously. Parallel driving of many CCDs together rules out the possibility of individual tuning; however, such optimisation is very important, when the ultimate low light level performance is required, particularly for new, or mixed devices. In this work, a new concept is explored for an entirely novel approach, where the drive waveforms are multiplexed and interleaved. This simultaneously reduces the number of leadout connections and permits individual optimisation efficiently. The digital controller can be designed within a single EPLD (Erasable Programmable Logic Device) chip produced by a CAD software package, where most of the digital controller circuits are integrated. This method can minimise the component. count., and improve the system efficiency greatly, based on earlier works by Han et a1. (1996, 1994). The system software has an open architecture to permit convenient modification by the user, to fit their specific purposes. Some variable system control parameters can be selected by a user with a wider range of choice. The digital controller design concept allows great flexibility of system parameters by the software, specifically for the compatibility to deal with any number of mixed CCDs, and in any format, within the practical limit.

• 한국통신학회논문지
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• 제18권9호
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• pp.1404-1411
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• 1993

음성 통신과는 달리 데이타 신호전송은 불규칙하게 순간적으로 발생하는 선로장애에 크게 영향을 받는다. 이런 장애로 인해 때로는 송수신기 사이의 동기를 잃게되어, 강제적으로 재 동기시키지 않는한. 회복이 스스로 되지 못한다. 따라서 이러한 순간적인 장애에 의한 동기 손실에 적절히 대처하기 위해 데이타통신 장비에 효율적인 검출기가 필요된다. 본 논문에서는 부속데이타 채녈이 주데이타 채널에 같이 통합되어있는 데이타 송수신기에 적당한 검출기를 설계하였다. 검출용 신호를 원래의 부속채널 데이타와 함께섞어 기존 채널의 운영에 영향이 없게 부속채널을 통해 보낸다. 데이타 전송중에 계속해서 동작하도록, 변형된 up/down 카운터를 이용한 검출방식을 사용한다. 설계된 검출기의 성능이 수학적으로 분석되었다.

• 한국통신학회논문지
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• 제35권12C호
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• pp.1044-1051
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• 2010

본 논문에서는 공간다중화 multiple input multiple output (MIMO) 시스템을 위한 효율적인 soft-output 신호검출 기법을 제안한다. 제안된 기법은 ordered successive interference cancellation (OSIC) 알고리즘을 기반으로 하지만, 오류 전파 문제를 크게 줄임으로써 기존 OSIC 알고리즘에 비해 큰 성능 향상을 보인다. 제안된 기법은 다중 순서화 기술을 이용한 enhanced OSIC (ESIC) 알고리즘을 결합한 기법으로 신뢰도 높은 log likelihood ratio(LLR) 값을 매우 적은 후보 심볼 벡터를 이용하여 생성할 수 있다. 본 논문에서는 $4{\times}4$ 16-QAM MIMO 시스템을 위한 OSIC, K-Best 가법과 제안된 신호검출 기법을 $0.13{\mu}m$ CMOS 기술 환경에서 구현하였으며, 모의 시험과 구현 결과를 통해 제안된 신호검출 기법이 성능과 하드웨어 구현 측면에서 매우 효율적임을 확인하였다.

• Bulletin of the Korean Chemical Society
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• 제35권5호
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• pp.1279-1284
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• 2014

The binding of TATA-binding protein (TBP) to the TATA-box containing promoter region is aided by many other transcriptional factors including TFIIA and TFIIB. The mechanistic insight into the assembly of RNA polymerase II preinitation complex (PIC) has been gained by either directly altering a function of target protein or perturbing molecular interactions using drugs, RNAi, or aptamers. Aptamers have been found particularly useful for studying a role of a subset of PIC on transcription for their ability to inhibit specific molecular interactions. One major hurdle to the wide use of aptamers as specific inhibitors arises from the difficulty with traditional assays to validate and determine specificity, affinity, and binding epitopes for aptamers against targets. Here, using a technique called the bio-layer interferometry (BLI) designed for a label-free, real-time, and multiplexed detection of molecular interactions, we studied the assembly of a subset of PIC, TBP binding to TATA DNA, and two distinct classes of aptamers against TPB in regard to their ability to inhibit TBP binding to TFIIA or TATA DNA. Using BLI, we measured not only equilibrium binding constants ($K_D$), which were overall in close agreement with those obtained by electrophoretic mobility shift assay, but also kinetic constants of binding ($k_{on}$ and $k_{off}$), differentiating aptamers of comparable KDs by their difference in binding kinetics. The assay developed in this study can readily be adopted for high throughput validation of candidate aptamers for specificity, affinity, and epitopes, providing both equilibrium and kinetic information for aptamer interaction with targets.

• 대한의생명과학회지
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• 제21권1호
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• pp.9-14
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• 2015

Mouse hepatitis virus (MHV) is a major pathogen in laboratory mice that usually leads to fatal diseases, such as hepatitis, multiple sclerosis, encephalitis, and respiratory disease. MHV has a high infection rate, and it needs to be detected as soon as possible to prevent its spread to other facilities. However, MHV detection by enzyme-linked immunosorbent assay (ELISA) often gives false positives; thus, it is very important that the results are confirmed as true positives in the early infection stage or distinguished as false positives with more accurate, reliable methods. Under microbiological screening, MHV ELISA-positive mice were found in four GFP-tagging transgenic mice. To verify the detection of the MHV antigen directly, reverse transcription polymerase chain reaction (RT-PCR) was performed, and the mice were determined to be MHV negative. Additional serum antibody-based screening was conducted with three different ELISA kits, and multiplexed fluorometric immunoassay (MFIA) was performed to confirm their accuracy/sensitivity. In brief, the ELISA kit for A59 nucleocapsid protein (MHV-A59N) revealed MHV ELISA positivity, while other ELISA kits (MHV-S lysate and MHV-JHM lysate) demonstrated MHV negativity. In MFIA, only the test for the recombinant A59 nucleocapsid antigen was MHV positive, which was consistent with the ELISA results. These results suggest that the ELISA kit with the recombinant A59 nucleocapsid antigen might induce non-specific MHV ELISA positivity and that confirmation is therefore essential.

• 대한전자공학회논문지TC
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• 제42권12호
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• pp.103-110
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• 2005

본 논문에서는 다중안테나를 갖는 공간 다중화 시스템에서 주어진 채널에 대한 maximum likelihood 검출기의 성능을 수식적으로 유도하였다. 벡터 심볼 오차율을 구하기 위해 nearest neighbor의 방향을 나타내는 에러벡터를 정의하였다. 전송 벡터 심볼이 랜덤한 채널에 의해 변환 될 경우 확정적으로 4개의 nearest neighbor를 가짐을 정의된 에러벡터의 특성을 이용해 입증하였다 변형된 구 검출기로부터 획득 가능한 에러벡터와 최소거리 정보를 바탕으로 주어진 랜덤 채널 하에서 벡터 심볼 오차율을 수식적으로 도출하였다. 유도된 벡터 심볼 오차식을 검증하기 위해, 랜덤 채널을 unitary 채널, dense 채널, sparse 채널로 분류한 후 각 채널 상황에서 유도된 결과 식을 모의 실험 결과와 비교하였다. 모의실험 결과로부터 유도된 벡터 심볼 오차식이 다양한 랜덤 MIMO 채널에서 ML 검출기의 성능을 잘 근사하고 있음을 입증하였다.