• Title/Summary/Keyword: Multiple-Chain

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Multiplex PCR for Detection of Quinolone Resistance qnr Genes in Extended-Spectrum β-Lactamase Producing Escherichia coli and Klebsiella pneumoniae (Multiplex PCR을 이용한 Extended-Spectrum β-Lactamase 생성 Escherichia coli와 Klebsiella pneumoniae의 Quinolone 내성 qnr유전자 검출)

  • Yang, Byoung-Seon
    • Korean Journal of Clinical Laboratory Science
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    • v.39 no.3
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    • pp.161-166
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    • 2007
  • To develop a rapid and reliable single-tube-based PCR technique for detection simultaneously the quinolone resistance qnrA, qnrB and qnrS genes. After multiple alignment, primers were designed to detect known qnr variants. I was used for A total of 43 extented-spectrum ${\beta}$-lactamases (ESBLs) producing Escherichia coli and Klebsiella pneumoniae isolated from university hospital were tested for screening, as with qnr genes. In optimized conditions, all positive controls confirmed the specificity of the PCR primers. Out of 43 isolates, qnrA genes were detected 19 (44.2%), qnrB genes 5 (11.7%), qnrS genes 15 (34.9%) and 8 (18.6%) isolates were not detected. I report here a fast and reliable technique for rapid screening of qnr positive strains to be used for epidemiological surveys.

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cDNA Cloning of Farnesoic Acid-Induced Genes in Candida albicans by Differential Display Analysis

  • CHUNG SOON-CHUN;LEE JI-YOON;OH KI-BONG
    • Journal of Microbiology and Biotechnology
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    • v.15 no.5
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    • pp.1146-1151
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    • 2005
  • The yeast Candida albicans has a distinguishing feature, dimorphism, which is the ability to switch between two morphological forms: a budding yeast form and a multicellular invasive filamentous form. This ability has been postulated to contribute to the virulence of this organism. Previously, we reported that the yeast-to-hypha transition in this organism is suppressed by farnesoic acid, a morphogenic autoregulatory substance that accumulates in the medium as the cells proliferate. In this study, using a differential display reverse transcription polymerase chain reaction (DDRT-PCR) technique, we have identified several genes induced in C. albicans by farnesoic acid treatment. These observations indicate that farnesoic acid can alter the expressivity of multiple genes, including the DNA replication machinery and cell-cycle-control proteins.

A Human Action Recognition Scheme in Temporal Spatial Data for Intelligent Web Browser

  • Cho, Kyung-Eun
    • Journal of Korea Multimedia Society
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    • v.8 no.6
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    • pp.844-855
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    • 2005
  • This paper proposes a human action recognition scheme for Intelligent Web Browser. Based on the principle that a human action can be defined as a combination of multiple articulation movements, the inference of stochastic grammars is applied to recognize each action. Human actions in 3 dimensional (3D) world coordinate are measured, quantized and made into two sets of 4-chain-code for xy and zy projection planes, consequently they are appropriate for applying the stochastic grammar inference method. We confirm this method by experiments, that various physical actions can be classified correctly against a set of real world 3D temporal data. The result revealed a comparatively successful achievement of $93.8\%$ recognition rate through the experiments of 8 movements of human head and $84.9\%$ recognition rate of 60 movements of human upper body. We expect that this scheme can be used for human-machine interaction commands in a web browser.

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Performance Analysis of a BRAM (The Broadcast Recognizing Aceess Method) Protocol in a Wireless LAN (무선 근거리 통신망에서의 BRAM(The Broadcast Recognizing Access Method) 프로토콜 성능 분석)

  • 김재현;이종규
    • Journal of the Korean Institute of Telematics and Electronics A
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    • v.31A no.1
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    • pp.1-8
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    • 1994
  • In this paper, we have analyzed the performance of a BRAM (The Broadcasting Recognizing Access Method) protocol, as a CSMA/CA (Carrier Sense Multiple Access/Collision Avoidance) scheme, which is widely used in wireles LAN systems. We have selected a Fair BRAM protocol among CSMA/CA schemes, considering the fairness of channel usage and the simplicity of the protocol. We have compared the performance of BRAM protocol to that of CSMA/CD. to research the characteristics of BRAM in wireless LAN system. In order to analyze the performance of this system, we have set up an imbedded Markov chain and calculated state transition probabilities. Then we have calculated steady state probabilities and finally derived the throughput of a Fair BRAM moder. To verify our analysis, we have simulated practical models. Then, we have found that analytic results are very close to simulation ones. Our analysis of the BRAM protocol will be expected to be very helpful to design and evaluate a MAC (Media Access Control) protocol in wireless LAN systems.

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WBAN MAC Protocols- Non-Saturation Modeling and Performance Analysis

  • Khan, Pervez;Ullah, Niamat;Kim, Hoon
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.11 no.3
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    • pp.1462-1476
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    • 2017
  • The current literature on discrete-time Markov chain (DTMC) based analysis of IEEE 802.15.6 MAC protocols for wireless body area networks (WBANs), do not consider the ACK timeout state, wherein the colliding nodes check the ill fate of their transmissions, while other contending nodes perform backoff check that slot as usual. In this paper, our DTMC model accurately captures the carrier sense multiple access with collision avoidance (CSMA/CA) mechanism of IEEE 802.15.6 medium access control (MAC) and allows the contending nodes performing backoff to utilize the ACK timeout slot during collisions. The compared rigorous results are obtained by considering a non-ideal channel in non-saturation conditions, and CSMA/CA parameters pertaining to UWB PHY of IEEE 802.15.6 MAC protocols.

An Empirical Investigation of Task-Technology Fit: Context of RFID in Disaster Management

  • Ahmed, Ashir
    • Asia pacific journal of information systems
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    • v.24 no.3
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    • pp.345-370
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    • 2014
  • The notion of technological adoption such as Radio Frequency Identification (RFID) has been examined in various domains such as supply chain management, inventory management and health care. However, there are several unanswered questions surrounding how this technology is adopted in disaster management. This study attempts to explore the potential of RFID in disaster management. The notion of Task-Technology Fit (TTF) is deemed suitable for this purpose and thus used as the theoretical framework that is further validated by employing multiple case studies. The empirical findings indicate that there are six key factors influencing the decision to adopt RFID in disaster management. Some relate to aspects of RFID when it is put into practice, namely cost, compatibility, standardisation, implementation and locatability; while the other key factor relates to privacy and security aspect of information. It is hoped that the findings of this research will inform disaster management organizations to better plan the adoption of RFID for their operations.

FUZZY REGRESSION TOWARDS A GENERAL INSURANCE APPLICATION

  • Kim, Joseph H.T.;Kim, Joocheol
    • Journal of applied mathematics & informatics
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    • v.32 no.3_4
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    • pp.343-357
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    • 2014
  • In many non-life insurance applications past data are given in a form known as the run-off triangle. Smoothing such data using parametric crisp regression models has long served as the basis of estimating future claim amounts and the reserves set aside to protect the insurer from future losses. In this article a fuzzy counterpart of the Hoerl curve, a well-known claim reserving regression model, is proposed to analyze the past claim data and to determine the reserves. The fuzzy Hoerl curve is more flexible and general than the one considered in the previous fuzzy literature in that it includes a categorical variable with multiple explanatory variables, which requires the development of the fuzzy analysis of covariance, or fuzzy ANCOVA. Using an actual insurance run-off claim data we show that the suggested fuzzy Hoerl curve based on the fuzzy ANCOVA gives reasonable claim reserves without stringent assumptions needed for the traditional regression approach in claim reserving.

A Study on SCM Connection using the Rosetta-net Standard (로제타넷표준을 적용한 SCM 연계에 관한 연구)

  • 서장훈;김용범;김우열
    • Journal of the military operations research society of Korea
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    • v.29 no.1
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    • pp.43-56
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    • 2003
  • This paper is intended to investigate the Build-up Method of SCM(Supply Chain Management) Connection applying EC Standard. SCM helps companies plan and supply to meet customer demand. SCM is the only solution available today that enables an organization to strategize, plan and execute a company's buy, make, move, store, fulfill and service business processes across multiple enterprises for total profitability. The research of modern scientists has thrown new light on the subject. However, the study of SCM for EC Standard has been strangely neglected by critics. Consequently, existing SCM systems, which require to introduce the functions of EC, need to be changed. Summarizations of the main points that have been analyzed in this paper are as follows:. the sharing information for connection and the Rosetta-net standard of the existing SCM system(Order management module(PIP 3A4) of the Rosetta-net specifications for connection by the Rosetta-net). Finally, it shows the process of the gateway for connecting among the existing SCM systems.

A Simulation Study for the Inventory Pooling Effect (재고풀링효과의 시뮬레이션 연구)

  • Jung, Jaeheon
    • Journal of Korean Society of Industrial and Systems Engineering
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    • v.35 no.4
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    • pp.211-218
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    • 2012
  • We analyzed the effect of inventory pooling on the system where multiple depot was used to replenish retailers and where inventories are kept only on the depots. Inventory pooling consists of inventory integration and inventory exchange. We used simulation for checking the cost saving effect of reducing the number of depot (Inventory Integration) for the case when inventories kept on every depots are commonly used for all retailers when certain depot have stock out for their retailer assigned to them (Inventory Exchange) with the constraint of service level. Simulation on wide range of parameter settings results show that cost saving effect from inventory integration diminishes when transportation cost between depot and retailers or stock out cost, or retailer number increases. The effect becomes stronger when the demands on retailers have bigger variance or average. Also the results show that the cost saving effect from inventory exchange becomes stronger on the same situation when inventory integration effect becomes stronger.

Coinfected cases with equine herpesvirus type 1, 4 and Streptococcus equi subsp. zooepidemicus in throughbred horse

  • Kim, Seong-Guk;Cho, Gil-Jae;Cho, Min-Hee;Kim, Young-Hoan;Lee, Hong-Young;Choi, Jeong-Hye;Kim, Jeong-Hwa;Choi, Seong-Kyoon
    • Korean Journal of Veterinary Service
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    • v.34 no.2
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    • pp.187-190
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    • 2011
  • The Thoroughbred horse was an approximately 4-years-old castrated male with highly emaciation, nasal epistaxis and subsequently died. Gross necropsy revealed epistaxis and hyperemia on the lung, multiple hemorrhage in muscle, and liver was focally attached to the peritoneum with fibrin. According to polymerase chain reaction (PCR), Equine herpes virus type 1 and 4 (EHV type 1, 4) was detected in the lung and trachea. In bacterial culture from kidney, liver, spleen, muscle and blood, Streptococcus equi subsp. zooepidemicus was isolated. Based on the gross lesion and PCR, this horse was diagnosed as EHV type 1, 4 and S. zooepidemicus coinfection.