• Journal of Microbiology and Biotechnology
• /
• v.29 no.12
• /
• pp.1916-1924
• /
• 2019

Outbreaks of staphylococcal food poisoning (SFP) causing serious human diseases and economic losses have been reported globally. Furthermore, the spread of Staphylococcus aureus with increased resistance to multiple antimicrobial agents has become a major concern in the food industries and medicine. Here, we isolated an endolysin LysSAP8, as one of the peptidoglycan hydrolases, derived from the bacteriophage SAP8 infecting S. aureus. This endolysin was tagged with a 6×His at the C-terminal of the target protein and purified using affinity chromatography. LysSAP8 demonstrated lytic activity against a broad spectrum of bacteria, which included a majority of the staphylococcal strains tested in this study as well as the methicillin-resistant S. aureus (MRSA); however, no such activity was observed against other gram-positive or gram-negative bacteria. Additionally, LysSAP8 could maintain bactericidal activity until 0.1 nM working concentration and after heat treatment at 37℃ for 30 min. The ability of LysSAP8 to lyse cells under varying conditions of temperature (4-43℃), pH (3-9), and NaCl concentrations (0-1,000 mM), and divalent metal ions (Ca²⁺, Co²⁺, Cu²⁺, Mg²⁺, Mn²⁺, Hg²⁺, and Zn²⁺) was examined. At the optimized condition, LysSAP8 could disrupt approximately 3.46 log CFU/ml of the planktonic cells in their exponential phase of growth within 30 min. In this study, we have suggested that LysSAP8 could be a potent alternative as a biocontrol agent that can be used to combat MRSA.

• Journal of the Korean Society of Safety
• /
• v.1 no.1
• /
• pp.51-59
• /
• 1986

This study was conducted to analyse the exact causes of occupational accidents in Korean construction industry and to contribute to the accident prevention programs. The results and conclusions of the field survey are as follows ： 1) The accidents caused by hazardous conditions are 96 percent, and those by unsafe acts are 97.3 percent. The accidents caused simultaneously by two categories are nore than 96 percent. 2) The injured workers who were employed less than 3 months are 71.1 percent. Safety training for newly recruited workers should be required. 3) More than 40 percent of all accidents were occurred in the morning and more than 30 percent were in summer. Required caution should be paid for the time and season. 4) Fracture, cut/laceration/puncture and multiple injuries in the lower extremeties, upper entremeties and head as well as many kinds of injuries by the fall from elevation mainly occurred. Safety. shoes, safety gloves, safety helmets, safety glasses, face shields and safity belts should be used 5) As the sources of injuries, each of building/structure and materials is one third of all sources, and machinery is a quarter. 6) The use of hazardous methods/procedures, defects of agencies and inadequate guarding of builing/structure, materials' and machinery mainly caused the accident types, such as s truck by, struck against, fall from elevation, and fall on same level. Such a hazardous conditiion should not be used and be correctet 7) The unsafe acts, such as improper use of hands or body parts, the operation or working at unsafe speed and improper use of equipment mainly caused accidents. Safety training for the control of such a unsafe acts should be strengthened.

• Journal of Internet Computing and Services
• /
• v.11 no.4
• /
• pp.143-158
• /
• 2010

The illegal leakage of enterprise digital confidential information will threaten the enterprise with bankruptcy. Today since most small-and-medium companies have no capability to fight against illegally compromising their critically confidential documents in spite of knowing the leakage of them, strongly safe distribution system of the digital confidential documents should be designed so in secure as to prevent any malicious intent of embezzlement from accessing the critical information. Current DRM-based protection system is not always perfect to protect the digital secrets, even seems to leave the secrets open. Therefore our study has analyzed the illegal leakage paths that hackers attack against and the vulnerability of the current protection systems. As result, we study the group communication based system architecture satisfying the security conditions to make even legitimate working employee keep out of the confidential documents, without performance degradation. The main idea of this architecture is to stay every secrets in encrypted form; to isolate the encrypted documents from the crypto-key; to associate every entity with one activity and to authenticate every entity with DSA-based public key system; multiple authentication method make hackers too busy to get a privilege to access the secrets with too many puzzle pieces. This paper deal with the basic architectural structure for the above issues.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.16 no.8
• /
• pp.5413-5421
• /
• 2015

The aim of this study was to identify the influences of sleep quality, depression and fatigue on job stress of geriatric hospital workers. The subjects were 175 workers from 6 geriatric hospitals. Data were collected on March, 2015 using a structured questionnaire. The data were analyzed by using SPSS/WIN 21.0 program. The job stress was negatively correlated with sleep quality(r=-.364, p<.001), positively correlated with depression(r=.376, p<.001) and fatigue(r=.646, p<.001). The multiple regression analysis revealed that the most powerful predictor of job stress was fatigue(${\beta}=.581$, p<.001). These results indicate that fatigue affect job stress of the geriatric hospital workers. Therefore, it is necessary to prepare tailored fatigue management program and working conditions that can reduce job stress.

• Korean Journal of Social Welfare
• /
• v.56 no.2
• /
• pp.311-326
• /
• 2004

This article focuses on the use of case management in the Self-support program. Case management is a service modality which provides integrated services for people with multiple needs in the community. The goal to accomplish self-sufficiency of working participants in Self-Support Programs is positively affected by the efforts to reduce high social risks they are exposed. It is recommended that the agencies running Self-support programs apply case management to increase the quality of lives as well as the effectiveness of exiting statuses of conditional welfare recipients. Several service considerations of case management are recommended for the change in the structure and functioning of the Self-Support Program: Integrated view and contextualized thinking, empowerment-oriented approach, and a development of the community support system through networking. Types of case management and practice roles of the case manager applicable to the Self-Support Program are also recommended. Structural aspects of an organization, inter-organizational linkages and contextual factors need to be considered to improve problematic conditions that have not enough resources and appropriate authorities in providing case management functions.

• Journal of Korean Port Research
• /
• v.13 no.2
• /
• pp.245-254
• /
• 1999

This paper aims to derive the strategies for the container terminal in Pusan. So it has focused on analyzing the current conditions of Pusan container terminal while evaluating the difficulties arising from the existing facilities/equipment and the operating system in the Pusan Korea Container Terminal through the workers at the container terminal and at off-dock CY. The object of this study is to assist the existing terminals to operate the facilities/equipment reasonably and to contribute shaping the ports policy which will be the basis of constructing new ports that will remain competitive while maintaining superiority over the ports in northeast area. According to the analysis 68% of the supplier answered that their terminal services which are the most important factor in running a terminal were superior to the other while only 28% of the user answered that they thought the current services were superior. For the areas of supplementing the terminal the people working at the terminal answered in the order of business operation equipment the number of berth service and management ability while the user answered in the order of service computer system transportation system and equipment. According to the regression analysis it became clear that there should be more effort toward improving or supplementing the facility scale facility usage charge transportation facilities which will assist in developing competitiveness of the terminal. It was also noted that management ability and business ability were mentioned as the important factors in operating a terminal.

• Korea journal of population studies
• /
• v.8 no.2
• /
• pp.93-99
• /
• 1985

There have been numerous studies in Korea and other countries of which the major hypotheses are identifying and dearibing the conditions under which the value of children has been formed. The present study proposes to view the formation of the value of children as a correlate of family's consumption-saving and work4eisure behavior pattern. The objectives of the proposed study are to identify the socio-economic and demographic factors determining the family's consumption-saving and work-leisure behavior pattern and to analyse the relationship between the value of children and this behavior pattern. The conceptual framwork of the analysis is that an individual family's socio-economic and demographic factors influence and shape the consumption-saving and work-leisure behaviors and these behaviors in turn influence and reflect the correlates and proximate determinants of the family'sfertility. In this paper, regression model is used to analyse the hypothesized relationship among the various variables. The regression methods used are first and second stage multiple regressions. In addition, MONOVA has been used to show the interactions. Data used are collected from the government publicactions. The major findings from this study are as follows: As the living Standard improves, n individual family's consumption of necessities and its working hours decline, while savings and leisure activities increase. The phenomena result in the fertility reduction as can be seen in the more developed conntries. Child-bearing and rearing activities are found to be the important component to determine the condumption-saving and work-leisure activies. The married women's labor participation, and the investment in education and health are also found to be the factors reducing fertility rate.

• The Journal of the Korea Contents Association
• /
• v.21 no.9
• /
• pp.595-608
• /
• 2021

The purpose of this study is to examine the effects of job stress and resilience on burnout of nurses who take care of patients with COVID-19 in prolonged pandemics. Structured questionnaires were distributed and analyzed for 162 nurses. Inadequate compensation of job stress, job demands, workplace culture, philosophical patterns of resilience, and relational patterns were statistically significant. As a result of determining the effect of job stress and resilience on burnout through the Stepwise multiple regression analysis, the final significant variable was job stress. The burnout of nurses can affect the quality of nursing services, reduced hospital competitiveness, and even quarantine systems. Therefore, this study suggests that proper compensation policy support, such as treatment of nurses and improvement of working conditions, is needed.

• Journal of Digital Convergence
• /
• v.19 no.2
• /
• pp.183-193
• /
• 2021

The purpose of this study was to verify how organizational fairness affects organizational commitment to social welfare officials, and to examine the mediating effect of organizational support. Social welfare officials have tried to find out what factors can be more adapted to the organization and immersed in the organizational aspect, as overwork and poor working conditions are becoming a problem. As the subject and method of the study, a questionnaire survey was conducted on 172 social welfare officials in P and U cities in northern Gyeonggi Province, and analyzed using multiple regression analysis. As a result of the study, organizational fairness of social welfare officials had a positive effect on organizational commitment, and organizational support had a partial mediating effect. Based on the results of this study, administrative and policy implications for the organizational adaptation and commitment of public officials in charge of social welfare were presented.

• Journal of dental hygiene science
• /
• v.23 no.4
• /
• pp.330-342
• /
• 2023

Background: As the medical knowledge base grows at an accelerating rate, evidence-based clinical performance becomes increasingly important for providing quality care. Previous studies have highlighted the need to promote job crafting to actualize evidence-based practical skills in the medical field. This study aimed to investigate the degree of evidence-based practice among dental hygienists and assess the impact of job crafting on the evidence-based practical skills of dental hygienists. Methods: Dental hygienists working at dental hospitals and clinics in Seoul and Gyeonggi Province were surveyed between February 28 and April 6, 2023. The sample was comprised of 267 participants. The hypotheses were tested independent t-tests, one-way analysis of variance, Pearson's correlation coefficients, and multiple regression analyses using SPSS 29.0. Results: The degree of job crafting by dental hygienists demonstrated significant differences based on educational attainment, workplace size, and workplace type. Evidence-based practical skills exhibited significant variations based on educational attainment and job position. All job crafting subfactors demonstrated positive correlations with evidence-based practical skills. The job crafting subfactors affecting the evidence-based practical skills of dental hygienists were 'increasing structural job resources' and 'increasing challenging job demands,' which together explained 38.7% of the variance in evidence-based practical skills. Conclusion: This study demonstrates that job crafting was positively and significantly correlated with evidence-based practical skills. To strengthen the job crafting ability of dental hygienists, improving environmental conditions and fostering an organizational culture that motivates continued participation in education is necessary. The development and promotion of programs that enable learning of the latest evidence should be actively pursued. Additionally, regular attendance at workshops and participation in organizational evidence-based practice education programs are necessary.