• Title/Summary/Keyword: Multiple layers

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Parthenogenetic Activation of Porcine Oocytes and Isolation of Embryonic Stem Cells-like Derived from Parthenogenetic Blastocysts

  • Xu, X.M.;Hua, J.L.;Jia, W.W.;Huang, W.;Yang, C.R.;Dou, Z.Y.
    • Asian-Australasian Journal of Animal Sciences
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    • v.20 no.10
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    • pp.1510-1516
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    • 2007
  • These experiments were carried out to optimize the parameters of electrical activation, methods of parthenogenetic activation and embryo culture in vitro and meanwhile to isolate embryonic stem cells-like (ESCs) derived from porcine parthenogenetic blastocysts (pPBs). These results showed that, as the electric field strength increased from 1.0 to 2.7 kV/cm, the cleavage rate of parthenogenetic embryos increased gradually but the rate of oocyte lysis was significantly increased when using 2.7 kV/cm field strength. The rate of cleavage in 2.2 and 2.7 kV/cm groups was significantly increased in comparison with that of the 1.0 kV/cm group. A voltage field strength of 2.2 kV/cm DC was used to investigate blastocyst development following activation with a single pulse of 30 or $60-{\mu}sec$ pulse duration. The optimum pulse duration was 30-${\mu}sec$, with a blastocyst rate of 20.7%. Multiple pulses were inferior to a single pulse for blastocyst yield (8.0% vs. 29.9) (p<0.05). For porcine oocyte parthenogenetic activation methods, the rates of cleavage (79.0% vs. 59.8%) and blastocysts (19.4% vs. 3.4%) were significantly increased in electrical activation in contrast to chemical activation with ionomycin/6-DMAP (p<0.05). Rates of cleavage and blastocyst formation in NCSU-23 and PZM-3 embryo media were higher than those of G1.3/G2.3 serial culture media, but there was no significant difference among the three groups. The total cell number of blastocysts in PZM-3 embryo culture media containing $5{\mu}g/ml$ insulin was significantly higher than that of the control (no insulin) ($44.3{\pm}9.1$ vs. $33.9{\pm}11.7$). For isolation of PESCs-like, the rates of porcine blastocysts attached to feeder layers and ICM colony formation in Method B (nude embryo culture) were better than those in Method A (intact embryo culture).

Reducing Flooding Latency in Power Save Mode of IEEE 802.11-based Mobile Ad hoc Networks (IEEE 802.11 기반 이동 애드혹 망의 전력 절감 모드에서 플러딩 지연의 개선)

  • 윤현주;서명환;마중수
    • Journal of KIISE:Information Networking
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    • v.31 no.5
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    • pp.532-543
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    • 2004
  • Mobile Ad hoc NETworks (MANET) consist of mobile nodes which are usually powered by battery Approaches for minimizing power consumption have been proposed for all network layers and devices. IEEE 802.11 DCF (Distributed Coordination Function), a well-known medium access control protocol for MANETS, also defines a power save mode operation. The nodes in power save mode periodically repeat the awake state and the doze state in synchronized fashion. When all nodes are in the awake state, the exchange the announcements for the subsequent message transmission with neighbors. The nodes that send or receive the announcements stay awake for data transmission, and others go into the dole state. The previous works for enhancing the power save mode operation have focused on shortening the duration of the awake state. We observed that the longer sleeping period results in seriously long delivery latency and the consequent unnecessary power consumption as well, because the packets can move forward only one hop for a fixed interval. In this paper, we propose an improved protocol for the power save mode of IEEE 802.11 DCF, which allows the flooding packets to be forwarded several hops in a transmission period. Our approach does not reduce the duration of compulsory awake period, but maximizes its utilization. Each node propagates the announcements for next flooding to nodes of several hops away, thus the packets can travel multiple hops during one interval. Simulation results of comparison between our scheme and the standard show a reduction in flooding delay maximum 80%, and the unicasting latency with accompanying flooding flows near 50%, with slight increase of energy consumption.

A Study of the Planting Characteristics of Street Trees and Herbaceous Plants in Gangwon-do (강원도 내 가로수와 가로녹지대 초화류의 식재 특성에 관한 연구)

  • Jeong Jin-Hyung;Lee Ki-Eui
    • Journal of the Korean Institute of Landscape Architecture
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    • v.33 no.5 s.112
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    • pp.57-68
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    • 2005
  • This study surveyed planting areas along streets in Gangwon-do to find out how to improve the planting and use of street trees and herbaceous plants. There were 301,491 trees of 41 species on the streets of Gangwon-do in 2004. The predominant species of street trees were Ginkgo biloba ($40\%$), Prunus spp. (Prunus yedoensis and Prunus sargentii) ($25\%$), Platanus occidentalis ($5\%$), followed by Betula platyphylla var. japonica, Zelkova serrata, Prunus armeniaca var. ansu, Acer palmatum, and Pinus thunbergii. Eighty-four herbaceous plant species were found in the Youngseo district (the southern area of Gangwon-do); the ratio of native species to exotic was 51:33. The predominant species were Cosmos bipinnatus, Petunia hybrida, Tagetes spp., Aster koraiensis, and Fagopyrum esculentum. Eighty-nine herbaceous plant species were found in the Youngdong district (the eastern area of Gangwon-do); the ratio of native species to exotic was 55:33. The predominant herbaceous plants were Aster koraiensis, Tagetes spp., Petunia hybrida, Rudbeckia bicolor, Cosmos bipinnatus, Salvia splendens, Brassica oleraceae var. acephala, Aquilegia buergeriana var. oxysepala, Coreopsis drummondii, Viola tricolor, and Dianthus superbus var. longicalycinus. Appropriate pruning adds to the aesthetic value of trees and prolongs their useful life; it also maintains good health and thereby reduces the need to control insects and diseases. Street trees had not been properly pruned due to the presence of power lines and a shortage of pruning information. The pruning was controlled by Korea Electric Power Company, which has no pruning information. Pruning must be maintained by a professional landscape company in order to maintain good shape, such as that which is done for bonsai. In order to improve the planting, use and maintenance of landscape plants in Gangwon-do, the following recommendations are made: street tree species should be diversified, suitable street trees should be selected for each space, native species should generally be used, trees should be appropriately pruned and properly fertilized, pests and diseases should be controlled, plantings should be done in multiple layers, spatial arrangements should be improved, larger trees should be planted, and drainage and underground electric wires should be considered when planting.

A Study on Random Selection of Pooling Operations for Regularization and Reduction of Cross Validation (정규화 및 교차검증 횟수 감소를 위한 무작위 풀링 연산 선택에 관한 연구)

  • Ryu, Seo-Hyeon
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.19 no.4
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    • pp.161-166
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    • 2018
  • In this paper, we propose a method for the random selection of pooling operations for the regularization and reduction of cross validation in convolutional neural networks. The pooling operation in convolutional neural networks is used to reduce the size of the feature map and for its shift invariant properties. In the existing pooling method, one pooling operation is applied in each pooling layer. Because this method fixes the convolution network, the network suffers from overfitting, which means that it excessively fits the models to the training samples. In addition, to find the best combination of pooling operations to maximize the performance, cross validation must be performed. To solve these problems, we introduce the probability concept into the pooling layers. The proposed method does not select one pooling operation in each pooling layer. Instead, we randomly select one pooling operation among multiple pooling operations in each pooling region during training, and for testing purposes, we use probabilistic weighting to produce the expected output. The proposed method can be seen as a technique in which many networks are approximately averaged using a different pooling operation in each pooling region. Therefore, this method avoids the overfitting problem, as well as reducing the amount of cross validation. The experimental results show that the proposed method can achieve better generalization performance and reduce the need for cross validation.

Epidemiological properties of salmonellosis of poultry farms in Kyongbuk province at recent year (최근 경북지역의 양계농장에서 발생한 salmonellosis의 역학적 특성)

  • 오강희;박노찬;김영환;조민희;이준규;신상희;손재권;정종식
    • Korean Journal of Veterinary Service
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    • v.23 no.1
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    • pp.45-59
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    • 2000
  • The present study was conducted to investigate the epidemiological properties of salmonellosis of poultry farms in Kyongbuk province during the relied from November 1998 to November 1999 And antibiotic susceptibility and biochemical characteristics of 120 Salmonella cultures isolated from chicken samples were also investigated. The results obtained through this study were summarized as follows, 1. Among 667,200 chickens of 31 flocks in 17 layer farms and 9 broiler farms, 61,350 chickens of 31 flocks were died with salmonellosis. The death rate of 25 farms varied from 0.1% to 75.0%, and the average death rate was 9.2%. 2. According to etiological agents, fowl typhoid was shown the most predominant outbreak among the salmonellosis during a year, which accounted for 8R.0%(22/25) of the total case 3. The serotypes of 120 Salmonella isolates were identified as 7 strains(5.8%) of S pullorum, 10 strains(8.4%) of S typhimurium and 103 strains(85.8%) of S gallinarum. 4. Most outbreak of fowl typhoid were prevalent on the layer chicken farms(77.1%), and the summer season(45.5%) also appeared the most hazardous season during the year. 5. It seemed that the Hyline breed(70.6%) was the most susceptible among the layer chicken breeds, and followed by Isabrown(23.5%), Tetra(5.9%) in order. 6. In layers, 76.4% of fowl typhoid occurred commonly from 14 to 40 weeks including the early laying peroid, but in broiler farms, all cases was outbreak within first second weeks. 7 All the strains of S pullorum were resistant to lincomycin(Lm), penicillin(Pm), and steptomycin(Sm), but sensitive to amikacin(Ak), ampicillin(Am), cephalothin(Ce), ciprofloxacin (CiP), chloramphenicol(Cm), colistin(Co), enrofloxacin(Enr), furazolidone(Fu), gentamicin(Gm), kanamycin(Km), neomycin(Nm), polymyxin(Po), and teracycline(Tc). All the strains of S typhimurium were resistant to Lm(100%), Pm(100%), Po(90%), and Sm(90%), but were sensitive to Ak, Am, Ce, CiP, Cm, Co, Enr, Fu, Gm, Km, Nm, and Tc. 8. Minimum inhibitory concentration(MIC) of 103 strains of S gallinarum were also evaluated and their patterns were much more variable than others. All the strains of S gallinarum were sensitive to Ak, Am, Ce, Cip, Cm, Enr, Fu, Km, and Nm, but resistant to Lm(100%), and Sm(100%), 99(96.1%) to Co, 83(80.6%) to Pm, and 83(80.6%) to Po, 55(53.4%) to Gm, and 33(32.0%) to Tc. 9. The multiple drug resistance patterns of 120 Salmonella strains were CoLmPmPo Sm pattern(34.2%), CoGmLmPmPoSmTc(20.8%), CoGmLmPmPoSm(13.3%), CoGmLmSm(7.5%), LmPmSm(7.5%), LmPm(6.7%), CoLmSmTc(3.3%),, CoGmLmSmTc(1.7%), GmLmSmTc(1.7%), CoGmLmPoSm(0.1%), LmPmPo(0.1%), CoLm Sm(0.1%), and LmSm(0.1%), in order.

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Via-size Dependance of Solder Bump Formation (비아 크기가 솔더범프 형성에 미치는 영향)

  • 김성진;주철원;박성수;백규하;이상균;송민규
    • Journal of the Microelectronics and Packaging Society
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    • v.8 no.1
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    • pp.33-38
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    • 2001
  • We investigate the via-size dependance of as-electroplated- and reflow-bump shapes for realizing both high-density and high-aspect ratio of solder bump. The solder bump is fabricated by subsequent processes as follows. After sputtering a TiW/Al electrode on a 5-inch Si-wafer, a thick photoresist for via formation it obtained by multiple-codling method and then vias with various diameters are defined by a conventional photolithography technique using a contact alinger with an I-line source. After via formation the under ball metallurgy (UBM) structure with Ti-adhesion and Cu-seed layers is sputtered on a sample. Cu-layer and Sn/pb-layer with a competition ratio of 6 to 4 are electroplated by a selective electroplating method. The reflow-bump diameters at bottom are unchanged, compared with as-electroplated diameters. As-electroplated- and reflow-bump shapes, however, depend significantly on the via size. The heights of as-electroplated and reflow bumps increase with the larger cia, while the aspect ratio of bump decreases. The nearest bumps may be touched by decreasing the bump pitch in order to obtain high-density bump. The touching between the nearest bumps occurs during the overplating procedure rather than the reflowing procedure because the mushroom diameter formed by overplating is larger than the reflow-bump diameter. The arrangement as zig-zag rows can be effective for realizing the flip-chip-interconnect bump with both high-density and high-aspect ratio.

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A Design of Smart Sensor Framework for Smart Home System Bsed on Layered Architecture (계층 구조에 기반을 둔 스마트 홈 시스템를 위한 스마트 센서 프레임워크의 설계)

  • Chung, Won-Ho;Kim, Yu-Bin
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.17 no.4
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    • pp.49-59
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    • 2017
  • Smart sensing plays a key role in a variety of IoT applications, and its importance is growing more and more together with the development of artificial intelligence. Therefore the importance of smart sensors cannot be overemphasized. However, most studies related to smart sensors have been focusing on specific application purposes, for example, security, energy saving, monitoring, and there are not much effort on researches on how to efficiently configure various types of smart sensors to be needed in the future. In this paper, a component-based framework with hierarchical structure for efficient construction of smart sensor is proposed and its application to smart home is designed and implemented. The proposed method shows that various types of smart sensors to be appeared in the near future can be configured through the design and development of necessary components within the proposed software framework. In addition, since it has a layered architecture, the configuration of the smart sensor can be expanded by inserting the internal or external layers. In particular, it is possible to independently design the internal and external modules when designing an IoT application service through connection with the external device layer. A small-scale smart home system is designed and implemented using the proposed method, and a home cloud operating as an external layer, is further designed to accommodate and manage multiple smart homes. By developing and thus adding the components of each layer, it will be possible to efficiently extend the range of applications such as smart cars, smart buildings, smart factories an so on.

A Study on Glucose Sensing Measured by Catalyst Containing Multiple Layers of Glucose Oxidase and Gold Nano Rod (글루코스산화효소와 금나노로드 입자의 다층막으로 구성된 촉매를 이용하여 측정한 글루코스 센싱에 대한 연구)

  • Chung, Yong-Jin;Hyun, Kyuhwan;Han, Sang Won;Min, Ji Hong;Chun, Seung-Kyu;Koh, Won-Gun;Kwon, Yongchai
    • Transactions of the Korean hydrogen and new energy society
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    • v.26 no.2
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    • pp.179-183
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    • 2015
  • In this study, we propose a catalyst structure including enzyme and metal nano rod for glucose sensing. In the catalyst structure, glucose oxidase (GOx) and gold nano rod (GNR) are alternatingly immobilized on the surface of carbon nanotube (CNT), while poly(ethyleneimine) (PEI) is inserted in between the GOx and GNR to fortify their bonding and give them opposite polarization ($[GOx/GNR]_nPEI/CNT$). To investigate the impact of $[GOx/GNR]_nPEI/CNT$ on glucose sensing, some electrochemical measurements are carried out. Initially, their optimal layer is determined by using cyclic voltammogram and as a result of that, it is proved that $[GOx/GNR/PEI]_2/CNT$ is the best layer. Its glucose sensitivity is $13.315{\mu}AmM^{-1}cm^{-2}$. When it comes to the redox reaction mechanism of flavin adenine dinucleotide (FAD) within $[GOx/GNR/PEI]_2/CNT$, (i) oxygen plays a mediator role in moving electrons and protons generated by glucose oxidation reaction to those for the reduction reaction of FAD and (ii) glucose does not affect the redox reaction of FAD. It is also recognized that the $[GOx/GNR/PEI]_3/CNT$ is limited to the surface reaction and the reaction is quasi-reversible.

Pre-Packing, Early Fixation, and Multi-Layer Density Analysis in Analytic Placement for FPGAs (FPGA를 위한 분석적 배치에서 사전 패킹, 조기 배치 고정 및 밀도 분석 다층화)

  • Kim, Kyosun
    • Journal of the Institute of Electronics and Information Engineers
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    • v.51 no.10
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    • pp.96-106
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    • 2014
  • Previous academic research on FPGA tools has relied on simple imaginary models for the targeting architecture. As the first step to overcome such restriction, the issues on analytic placement and legalization which are applied to commercial FPGAs have been brought up, and several techniques to remedy them are presented, and evaluated. First of all, the center of gravity of the placed cells may be far displaced from the center of the chip during analytic placement. A function is proposed to be added to the objective function for minimizing this displacement. And then, the density map is expanded into multiple layers to accurately calculate the density distribution for each of the cell types. Early fixation is also proposed for the memory blocks which can be placed at limited sites in small numbers. Since two flip-flops share control pins in a slice, a compatibility constraint is introduced during legalization. Pre-packing compatible flip-flops is proposed as a proactive step. The proposed techniques are implemented on the K-FPGA fabric evaluation framework in which commercial architectures can be precisely modeled, and modified for enhancement, and validated on twelve industrial strength examples. The placement results show that the proposed techniques have reduced the wire length by 22%, and the slice usage by 5% on average. This research is expected to be a development basis of the optimization CAD tools for new as well as the state-of-the-art FPGA architectures.

Histological healing after grafting of bilayer artificial dermis in the oral environment (치은부에 이식한 이중인공진피의 조직학적 치유)

  • Kim, Min-Jeong;Chung, Hyun-Ju;Kim, Ok-Su;Kim, Young-Joon
    • Journal of Periodontal and Implant Science
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    • v.33 no.2
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    • pp.289-299
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    • 2003
  • The autogenous free gingival graft is the most predictable procedure currently used to increase the width of the attached gingiva in periodontics. But the major disadvantage of the procedure is to create the multiple surgical wounds at both a donor site and a recipient site. The other problem is the limited amount of available graft material in oral cavity. Therefore, recent researches have been focused to develop the biomaterial to substitute the autogenous gingival tissue. The purpose of this study was to evaluate the histologic healing after grafting of bilayer artificial dermis, compared to the free gingival graft. Four non-smoking subjects (mean age, 32.5 years) in systemically healthy state and good oral hygiene were selected according to their particular needs for correction of mucogingival problems as suggested by Nabers(1966). The recipient sites were prepared through the procedure for the free gingival graft and were grouped according to the graft materials: Experimental group(n=5) - bilayer artifcia1 dermis ($Terudermis^{(R)}$; Terumo Co. Japan) and Control group(n=6) - free gingival graft with autogenous palatal mucosa. Biopsies were harvested at 1,2,3 and 6 weeks postsurgery to evaluate histologically. At the third week in the experimental group and at the second week of in the control group, the grafts has been clinically stabilized on the recipient bed and the graft border has been blended into the surrounding tissue. In the experimental group after 1 week of grafting, the epithelial migration from the adjacent tissue to graft material was seen and after 3 weeks of grafting, the : nflmmation decreased, collagen layer of the artificial dermis was lost and the basement membrane of epithelium was formed. After 6 weeks of grafting, both groups demonstrated orthokeratinized epithelium and increased thickness of epithelial tissue and the rete peg formation, similar to the adjacent tissue, Histologic evaluation revealed a biologic acceptance and incorporation of the collagen layers of the graft tissue to the host tissue, without foreign body reaction. In conclusion, a bilayer artificial dermis is essentially similar to autogeneous free gingival graft in the correction of mucogingival problems, and has the advantages of decreased patient morbidity (no donor site) and availability of abundant amounts of graft material when needed.