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http://dx.doi.org/10.7316/KHNES.2015.26.2.179

A Study on Glucose Sensing Measured by Catalyst Containing Multiple Layers of Glucose Oxidase and Gold Nano Rod  

Chung, Yong-Jin (Graduate School of Energy and Environment, Seoul National University of Science and Technology)
Hyun, Kyuhwan (Graduate School of Energy and Environment, Seoul National University of Science and Technology)
Han, Sang Won (Department of Chemical and Biomolecular Engineering, Yonsei University)
Min, Ji Hong (Department of Chemical and Biomolecular Engineering, Yonsei University)
Chun, Seung-Kyu (Graduate School of Energy and Environment, Seoul National University of Science and Technology)
Koh, Won-Gun (Department of Chemical and Biomolecular Engineering, Yonsei University)
Kwon, Yongchai (Graduate School of Energy and Environment, Seoul National University of Science and Technology)
Publication Information
Journal of Hydrogen and New Energy / v.26, no.2, 2015 , pp. 179-183 More about this Journal
Abstract
In this study, we propose a catalyst structure including enzyme and metal nano rod for glucose sensing. In the catalyst structure, glucose oxidase (GOx) and gold nano rod (GNR) are alternatingly immobilized on the surface of carbon nanotube (CNT), while poly(ethyleneimine) (PEI) is inserted in between the GOx and GNR to fortify their bonding and give them opposite polarization ($[GOx/GNR]_nPEI/CNT$). To investigate the impact of $[GOx/GNR]_nPEI/CNT$ on glucose sensing, some electrochemical measurements are carried out. Initially, their optimal layer is determined by using cyclic voltammogram and as a result of that, it is proved that $[GOx/GNR/PEI]_2/CNT$ is the best layer. Its glucose sensitivity is $13.315{\mu}AmM^{-1}cm^{-2}$. When it comes to the redox reaction mechanism of flavin adenine dinucleotide (FAD) within $[GOx/GNR/PEI]_2/CNT$, (i) oxygen plays a mediator role in moving electrons and protons generated by glucose oxidation reaction to those for the reduction reaction of FAD and (ii) glucose does not affect the redox reaction of FAD. It is also recognized that the $[GOx/GNR/PEI]_3/CNT$ is limited to the surface reaction and the reaction is quasi-reversible.
Keywords
Glucose oxidase; Gold nano rod; glucose sensing; Glucose sensitivity; Glucose oxidation reaction;
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