• 수산해양기술연구
• /
• 제58권1호
• /
• pp.85-93
• /
• 2022

In this study, we investigated how satisfaction with on-board training had an effect on career motivation of the trainees. For this purpose, satisfaction for on-board training and career motivation were used as variables. Satisfaction for on-board training were classified into education, environment and operation, and then measurement items were composed. Career motivation consisted of career identity, career insight and career resilience. To establish and test nine hypotheses, a questionnaire survey was conducted by 57 trainees who participated in the Gyeongsang National University on-board sailing training in 2021. To analyze the questionnaire responses, frequency analysis, factor analysis, multiple regression analysis and paired sample t-test were used. As a result of factor analysis, six factors for satisfaction for on-board training were derived. Among the factors, it was analyzed that satisfaction with assignments, the satisfaction with accommodation, and the satisfaction with the adequate condition of facilities and equipment had a significant effect on career motivation. In addition, it was found that career motivation significantly increased through on-board training as a result of comparing the difference between the averages of career motivation before and after on-board training.

• Journal of Microbiology and Biotechnology
• /
• 제33권5호
• /
• pp.668-679
• /
• 2023

Edwardsiella tarda is one of the most significant fish pathogens, causes edwardsiellosis in a variety of freshwater fish species, and its antibiotic resistance against multiple drugs has made it a health risk worldwide. In this study, we aimed to investigate the antibiotic resistance (ABR) genes of E. tarda and establish its antibiotic susceptibility. Thus, 540 fish (299 Oreochromis niloticus, 138 O. mossambicus, and 103 O. aureus) were collected randomly from twelve fish farms in three districts of Punjab in Pakistan. E. tarda was recovered from 147 fish showing symptoms of exophthalmia, hemorrhages, skin depigmentation, ascites, and bacteria-filled nodules in enlarged liver and kidney. Antimicrobial susceptibility testing proved chloramphenicol, ciprofloxacin, and streptomycin effective, but amoxicillin, erythromycin, and flumequine ineffective in controlling edwardsiellosis. Maximum occurrence of qnrA, blaTEM, and sul3 genes of E. tarda was detected in 45% in the liver, 58%, and 42% respectively in the intestine; 46.5%, 67.2%, and 55.9% respectively in O. niloticus; 24%, 36%, and 23% respectively in summer with respect to fish organs, species, and season, respectively. Motility, H₂S, indole, methyl red, and glucose tests gave positive results. Overall, E. tarda infected 27.2% of fish, which ultimately caused 7.69% mortality. The Chi-squared test of independence showed a significant difference in the occurrence of ABR genes of E. tarda with respect to sampling sites. In conclusion, the misuse of antibacterial agents has led to the emergence of ABR genes in E. tarda, which in association with high temperatures cause multiple abnormalities in infected fish and ultimately resulting in massive mortality.

• 수산해양기술연구
• /
• 제51권2호
• /
• pp.235-244
• /
• 2015

The multi-frequency characteristics of anchovy schools were investigated using six acoustic lines collected at 38 and 120 kHz while a primary trawl survey was conducted from 14 April and 18 April of 2014 in off the coast of Tongyeong and Geo-je. Here, the frequency characteristics mean ${\Delta}MVBS$ that is the difference of Mean Volume Backscattering Strength at two frequencies. To use the characteristics effectively, the optimal cell size ($10{\times}2m$) was determined by examining several different cell sizes in consideration with the shapes of fish schools and the ${\Delta}MVBS$ pattern. By examining 6 histograms of ${\Delta}MVBS$, afternoon groups were occupied more in the ${\Delta}MVBS$ range of -6~-4 dB than that of -4~-2 dB, comparing to morning groups. The ${\Delta}MVBS$ range of the morning groups was between -16.9 dB and 11.6 dB, and that of the afternoon groups -16.7 dB and 13.0 dB. The average and standard deviation were $-3.9{\pm}3.6$ dB in the morning and $-4.1{\pm}3.4$ dB in the afternoon, suggesting that morning groups were 2 dB higher than afternoon groups. The ${\Delta}MVBS$ range of all anchovy schools regardless of morning and afternoon was between -16.9 dB and 13.0 dB, their average ${\Delta}MVBS$ was $-4.1{\pm}3.5$ dB. The characteristics can support to identify anchovy species in the waters where multiple fish species are distributed. It is hoped that this study presents the availability and benefit of acoustic data from a primary trawl survey.

• 생명과학회지
• /
• 제26권9호
• /
• pp.1007-1014
• /
• 2016

본 연구에서는 국산 참서대과 어류 5종(개서대, 참서대, 칠서대, 박대, 용서대) 및 수입산 참서대과(Cynoglossidae) 어류 5종(기니개서대, 긴개서대, 큰비늘개서대, 큰입개서대, 세네갈 개서대)을 대상으로 분자생물학적 방법을 이용한 신속하고 정확한 종판별법을 검토하였다. 참서대과 어류 10종에 대한 최적의 종 특이 프라이머를 선정하기 위하여 약 1,500 bp의 COI 유전자를 분석하였으며, 종간 특이적인 단일염기다형성 유전자가 3’ 말단에 위치하도록 프라이머를 제작하였다. 제작된 10종에 대한 종특이 정방향 프라이머는 동일한 PCR조건과 전기영동을 통해 육안으로 식별이 가능할 정도의 PCR 산물의 상대적인 크기를 고려하였다. 다중 PCR 분석을 위해 종특이 정방향 프라이머는 모두 혼합되어 사용되었으며, 그 결과 세네갈개서대(208 bp), 용서대(322 bp), 큰입개서대(493 bp), 큰 비늘개서대(754 bp), 박대(874 bp), 칠서대(952 bp), 참서대(1,084 bp), 긴개서대(1,198 bp), 개서대(1,307 bp), 기니개서대(1,483 bp)에 해당하는 종 특이적인 증폭을 확인하였다. 또한 이들의 PCR 민감도를 측정한 결과 0.1~1.0 ng/μ l의 농도까지 검출이 가능한 것을 확인 하였다. 본 연구에서 확인된 참서대과 어류 10종에 대한 종특이 프라이머는 특이도 및 민감도가 우수하며 향후 수산물의 수출입 및 유통 관리에 사용이 이루어진다면 정확한 종명 표기가 가능하여 국민 먹거리 안전을 위한 효과적인 방법이 될 것이다.

• 수산해양교육연구
• /
• 제27권5호
• /
• pp.1252-1264
• /
• 2015

This study examined the moderating and mediating effects of teacher-child conflict relationship among child's language ability, emotional regulation and peer victimization. The participants were 152 children(77 boys, 75 girls) and 14 preschool teachers. The teachers completed rating scales to measure the child's emotional regulation, peer victimization and teacher-child relationship. Child's language ability was assessed by researcher using PRES(Preschool Receptive-Expressive Language Scale). The collected data were analyzed using Pearson correlations and hierarchical multiple regressions. Results showed that peer victimization was related to child's language ability, emotional regulation and teacher-child relationship. Hierarchical aggression analysis indicated that the interaction of child's receptive language ability and teacher-child conflict relationship predicated peer victimization. Child's language ability, whose demonstrated a lower teacher-child conflict relationship, was significantly with peer victimization. In addition, the association between a child's emotional regulation and peer victimization was partially mediated by teacher-child conflict relationship.

• 수산해양교육연구
• /
• 제27권5호
• /
• pp.1273-1289
• /
• 2015

The purpose of this study was to use the parent of the infant status and their media perception (3-7 five years) the infants' media use by examining the situation looking at the parents' use of media that cause impact on infants' media use parents will be validated accordingly to seek the solutions for the media and the involvement of infants perception. U City and 14 practitioners in preschool infants aged 5-7 target parents from 1307 people based in September 2014, a survey was conducted two months until October. This study was a mixed Methods for quantitative analysis of all the results and qualitative analysis of the results. Results for media use quantitative analysis of the results and impact parents have on infants' media use their media usage status of the parents and the media use of infants by their perceived status has been subjected to frequency analysis and hierarchical multiple regression analysis, parents perception results. Analysis of the solution for the immersion of the media and the infants unit of analysis was used for componential analysis. Through this study, parents would like to establish a plan to take advantage of essential educational content in the media and sought ways for the correct use of infants in the media and practice at home.

• 한국수산과학회지
• /
• 제37권5호
• /
• pp.405-413
• /
• 2004

The previous morphology-based taxonomic frameworks within the family Syngnathidae had emphasized the significance of the male brood pouch and reproductive biology in defining the group. However, several different hypotheses had been proposed by different investigators. This study has been carried out to determine the phylogenetic relationships among 19 species belonging to the order Syngnathiformes with three Gasterosteiformes species as outgroup taxa by using the mitochondrial cytochrome b DNA sequences. Phylogenetic analyses based on neighbor-joining distance, maximum parsimony, minimum evolution and maximum likelihood method strongly supported that the family Syngnathidae, the suborder Syngnathoidei and the order Syngnathiformes were all monophyletic group. Although much of previous morphological analyses were supported by our molecular data, there were some significant discrepancies between molecular and morphological work. Such an interesting result was that the weedy seadragon (Phyllopteryx taeniolatus) strongly grouped together with the New Zealand pot-belly seahorse (Hippocampus abdominalis). Considering the markedly different brooding structure between them, this unexpected result might be explained whether by multiple independent origins of brooding structure or by hybridization between the female Hippocampus and other syngnathid species having individual membranous egg compartment. In addition, the suborder Aulostomoidei was paraphyletic group because the shrimpfish (Aeliscus strigatus), belonging to the family Centriscidae, always grouped together with the family Syngnathidae as a sister taxon.

• 수산해양기술연구
• /
• 제44권1호
• /
• pp.31-36
• /
• 2008

The underwater sound transmission system(USTS) was experimentally made to monitor the cetacean's appearance by telemetry, and then its system was tested to evaluate its performance from July to October, 2007 at the Kimnyeong berth and the dolphin's breeding ground of Pacific Land in Jeju island, respectively. The results showed that the sweep sound in the trial experiment and the whistle sound of bottlenose dolphin(Tursiops truncatus) were favorably received by telemetry. Therefore, we could confirm the USTS is able to monitor the cetacean's appearance in real time without direct observation at sea within effective range of code division multiple access(CDMA) communication method.

• Fisheries and Aquatic Sciences
• /
• 제12권3호
• /
• pp.185-193
• /
• 2009

A complete cDNA, which encodes for a myostatin-like protein (Es-MSTN), was isolated from the Chinese mitten crab, Eriocheir sinensis. Es-MSTN was composed of 2,397 nucleotides and the open reading frame (ORF) specified a protein containing 468 amino acids. Es-MSTN exhibited 32% amino acid sequence identity and 52% similarity to human myostatin. Multiple sequence alignment analysis indicated that Es-MSTN possessed the conserved proteolytic cleavage site (RXXR) for maturation of the protein and nine cysteine residues for disulfide bridges. Besides the conserved structural features, Es-MSTN also exhibits its unique characters; a longer N-terminal domain which is involved in protein folding and latent form of myostatin and absence of the cleavage site for BMP-1/tolloid family of metalloproteinase to activate mature myostatin. Phylogenetic analysis suggests that Es-MSTN showed the closely related to both vertebrate myostatin and GDF11. Es-MSTN is expressed highly in the claw muscle, leg muscle, thoracic muscle and heart, and moderately in the hindgut suggesting that Es-MSTN may play important roles in the muscle tissues. As homolog of mammalian myostatin and GDF11, Es-MSTN may be involved in development of muscular tissue and further study will help to produce high-quality seafood.

• 수산해양교육연구
• /
• 제27권1호
• /
• pp.74-82
• /
• 2015

The Admission Officer System is a selecting system that evaluates potential and talent of students in multiple aspects and selects whom each university wants to pick and fits the specific recruitment units. However, the issue about fairness of the system has been discussed continually. This study conducted online survey, using fairness checklist with 31 universities to know how admission officers perceive the fairness of the system and secure that. Also, three times of expert FGI were conducted, too. The result shows there are still many universities that adjustment for deviated evaluation in interview, separation of operating agency, stabilization of the statue of admission officers, analyzation of each evaluator's tendency, and so on are not achieved well. To assure the fairness of the admission officer system, securing validity by clearly defining the concept of fairness is proposed first. Stabilizing the statue of admission officers and continuous education training for them is also proposed to improve their professionality. Furthermore, expansion of education programs about the admission officer system in both quantity and quality is suggested for fairness of the system.