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http://dx.doi.org/10.5657/fas.2009.12.3.185

A Myostain-like Gene Expressed Highly in the Muscle Tissue of Chinese mitten crab, Eriocheir sinensis  

Kim, Kyoung-Sun (Department of Marine Biology, Pukyong National University)
Jeon, Jeong-Min (Department of Marine Biology, Pukyong National University)
Kim, Hyun-Woo (Department of Marine Biology, Pukyong National University)
Publication Information
Fisheries and Aquatic Sciences / v.12, no.3, 2009 , pp. 185-193 More about this Journal
Abstract
A complete cDNA, which encodes for a myostatin-like protein (Es-MSTN), was isolated from the Chinese mitten crab, Eriocheir sinensis. Es-MSTN was composed of 2,397 nucleotides and the open reading frame (ORF) specified a protein containing 468 amino acids. Es-MSTN exhibited 32% amino acid sequence identity and 52% similarity to human myostatin. Multiple sequence alignment analysis indicated that Es-MSTN possessed the conserved proteolytic cleavage site (RXXR) for maturation of the protein and nine cysteine residues for disulfide bridges. Besides the conserved structural features, Es-MSTN also exhibits its unique characters; a longer N-terminal domain which is involved in protein folding and latent form of myostatin and absence of the cleavage site for BMP-1/tolloid family of metalloproteinase to activate mature myostatin. Phylogenetic analysis suggests that Es-MSTN showed the closely related to both vertebrate myostatin and GDF11. Es-MSTN is expressed highly in the claw muscle, leg muscle, thoracic muscle and heart, and moderately in the hindgut suggesting that Es-MSTN may play important roles in the muscle tissues. As homolog of mammalian myostatin and GDF11, Es-MSTN may be involved in development of muscular tissue and further study will help to produce high-quality seafood.
Keywords
Myostatin; Muscle; Chinese mitten crab; Eriocheir sinensis; Crustacean; Molting;
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