• Korean Journal of Clinical Laboratory Science
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• v.47 no.3
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• pp.140-146
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• 2015

The increase in resistance by pathogenic bacteria to multiple antimicrobial agents has become a significant treat, as the effective antimicrobial agents available for the patients infected by such resistant bacteria are reduced, or even eliminated. Several natural plant extracts have exhibited antibacterial and synergistic activity against various resistant microorganisms. Houttuynia cordata is frequently used by many traditional medicine practicioners for its antimicrobial, antiviral, and anti-inflammatory properties. This study investigated the antibacterial effects of H. cordata extract against clinical multi-resistant bacteria, and compared the two methods used for the antimicrobial susceptibility testing. Thirty isolates of Methicillin-resistant Staphylococcus aureus (MRSA, 10), Vancomycin-resistant Enterococcus faecium (VRE, 10), Carbapenem-resistant Acinetobacter baumannii (CRAB, 10) were included in this study. The antibacterial effect of H. cordata was tested by disk diffusion and microbroth dilution methods as per CLSI guidelines. In disk diffusion, all isolates (30) showed no inhibition to 30,000 ug/mL of H. cordata. But in the microbroth dilution method, $MIC_{90}$ of H. cordata was 4,096 ug/mL, 8,192 ug/mL and 4,096 ug/mL in MRSA, VRE and CRAB, respectively. These results demonstrate that H. cordata exhibits antibacterial activity against MRSA, VRE and CRAB. Moreover, the microbroth dilution method is a more effective method than disk diffusion to evaluate the antibacterial activity of natural products. The Disk diffusion method used to evaluate the antibacterial activity of natural products required new standard guidelines including inoculum concentration of bacteria.

• Ecology and Resilient Infrastructure
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• v.6 no.4
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• pp.227-235
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• 2019

This study monitored Daecheong 1-bo, Daecheong-stream, which carried out the project in 2014, from 2015 to 2016. The technology applied to the stream was evaluated using Periphytic Algae to check contamination indicators and ecological health of the area with an integral river-bed protection using non-toxic materials. The water quality of the monitoring section was confirmed to be above the river environment standard (II), and it was confirmed that the Saproxenic taxa of the river bed protection were higher than the upper and downstream sections. The TDI, which is an index of attachment algae, was shown in the average 51.03 and 52.15 for the pilot project sections in 2015 and 2016, confirming that the index is of the "normal" grade. This is the other sections in the upstream and downstream sections showed higher than "bad", which is thought to have a positive effect on the habitat of the river ecosystem components, especially the microbial population in river bed protection.

• Journal of Environmental Impact Assessment
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• v.27 no.3
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• pp.241-250
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• 2018

Recently, negative effects on human health such as disease caused by harmful environment have been dealt with seriously. In particular, studies on the effect of radon exposure, which is known as a primary carcinogen in lung cancer due to radioactive materials, have been actively studied. In Korea, since January 1, 2018, radon measurement is mandatory when building a new apartment, so it is necessary to measure the radon concentration and submit it to the local government and it should be posted where residents can see it. Radon has only recommended standards for multi-use facilities, but now it has decided to set recommendation standards for private homes. Therefore, it should now be possible to manage the radon in the environmental impact assessment phase as well as in the Post-environmental Impact Assessment. It should be possible to share health information such as the radon concentration and the risk of radon, and participation of health experts in the environmental impact assessment stage is required. Soil, air quality, hygiene and aerial items should be improved to take into account the effects of radon on human health during the environmental impact assessment process. If the level value of conncentration of radon shows above the recommended level, then alternative measures should be prepared and mitigation measures should be prepared as well.

• Journal of Embryo Transfer
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• v.27 no.1
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• pp.57-62
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• 2012

Efficient oocyte activation is a key step for the success of nuclear transfer in cloning. Ionomycin sequentially combined with 6-DMAP is now widely used to activate normal oocytes for analytical studies of oocyte activation and to activate reconstructed oocytes after nuclear transfer. The present study investigated sources of oocytes, duration of ionomycin and 6-DMAP, laser and electric stimulation in goat oocyte activation in order to optimize the protocols. Goat ovaries were collected in individual abattoirs during the breeding season and were delivered to the laboratory within 6 h in saline with 100 IU/ml streptomycin and 0.05 mg/ml penicillin. The oocytes were denuded from the cumulus cell by pipetting with 0.2% hyaluronidase in PBS at 20~22 hr post maturation. Oocytes with the polar body were selected and assigned to four groups for parthenogenetic activation. To examine the effect of duration of ionomycin treatment, oocytes after 20~22 hr of maturation were treated with 2.5 uM ionomycin for 1 or 5 min times and then cultured in 2 mM 6-DMAP for 2 or 4 hr. The activated oocytes were cultured in mSOF at $38.5^{\circ}C$ in $CO_2$ 5%, $O_2$ 5% and $N_2$ 90% multi incubator. Cleavage and blastocyst development was observed at 48 hr and day 8 of culture $in$ $vitro$, respectively. Activation rates of oocytes exposed to ionomycin for 1 min(86.4%) were significantly higher than those treated for 5 min(74.3%) duration. This indicated that 1 min ionomycin treatment was most suitable for activation of goat oocytes. The duration of 6-DMAP treat duration was in 2 mM 6-DMAP for 2 hr after 1 min exposure to 2.5 uM ionomycin. The activation rate of oocytes incubated in 6-DMAP for 2 hour(82.5%) was significantly higher than those in oocytes treated with 4 hr(75.5%).

• Korean Journal of Acupuncture
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• v.30 no.3
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• pp.161-170
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• 2013

Objectives : Our study aimed to investigate the sustained effects of sham (SHAM) and verum acupuncture (ACUP) into the post-stimulus resting state. Methods : In contrast to previous studies, in order to define the changes in resting state induced by acupuncture, changes were evaluated with a multi-method approach by using regional homogeneity (ReHo) and amplitude of low frequency fluctuation (ALFF). Twelve healthy participants received SHAM and ACUP stimulation right GB34 (Yanglingquan) and the neural changes between post- and pre-stimulation were detected. Results : The following results were found; in both ReHo and ALFF, the significant foci of; left and right middle frontal gyrus, left medial frontal gyrus, left superior frontal gyrus, and right posterior cingulate cortex, areas that are known as a default mode network, showed increased connectivity. In addition, in ReHo, but not in ALFF, brain activation changes in the insula, anterior cingulate cortex, and the thalamus, which are associated with acupuncture pain modulation, were found. Conclusions : In this study, results obtained by using ReHo and ALFF, showed that acupuncture can modulate the post-stimulus resting state and that ReHo, but not ALFF, can also detect the neural changes that were induced by the acupuncture stimulations. Although more future studies with ReHo and ALFF will be needed before any firm conclusions can be drawn, our study shows that particularly ReHo could be an interesting method for future clinical neuroimaging studies on acupuncture.

• The Journal of the Korea Contents Association
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• v.12 no.9
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• pp.396-407
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• 2012

The Purpose of this study was to examined the relation between Belief and Actual Practice of Instructor according to education in Taekwondo Personality. To get the purpose participants was selected 286 Taekwondo instructors. Selected data were analyzed for descriptive statistic, pearson relation analysis, and multi dimensional analysis with SPSS/PC 18.0. The result was as followed. First, there were different on frequency of important factors between belief and practice of Taekwondo personality based on Taekwondo instructor's cognitive. The order of ethological important factors on Taekwondo instructor's cognitive were manner, confidence, love, perseverance, bravery. But The order of ethological important factors on Taekwondo instructor's practice were manner, confidence, perseverance, leadership, honestly. Second, belief of Taekwondo personality based on Taekwondo instructor's cognitive has positive relationship with practice of Taekwondo personality in a part. There were positive relationship among factors of belief of Taekwondo personality but were lower positive relationship among factors of practice of Taekwondo personality. Taekwondo instructors' determination on teaching belief has positive effect on guidance factor, practical factor, variety factor, if the teaching belief has higher score then the teaching practice has higher practical teaching.

• Korean Journal of Construction Engineering and Management
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• v.16 no.2
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• pp.65-76
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• 2015

The Clean Development Mechanism (CDM) is the multi-lateral 'cap and trade' system endorsed by the Kyoto Protocol. CDM allows developed (Annex I) countries to buy CER credits from New and Renewable (NE) projects of non-Annex countries, to meet their carbon reduction requirements. This in effect subsidizes and promotes NE projects in developing countries, ultimately reducing global greenhouse gases (GHG). To be registered as a CDM project, the project must prove 'additionality,' which depends on numerous factors including the adopted technology, baseline methodology, emission reductions, and the project's internal rate of return. This makes it difficult to determine ex ante a project's acceptance as a CDM approved project, and entails sunk costs and even project cancellation to its project stakeholders. Focusing on hydro power projects and employing UNFCCC public data, this research developed a prediction model using logistic regression and CART to determine the likelihood of approval as a CDM project. The AUC for the logistic regression and CART model was 0.7674 and 0.7231 respectively, which proves the model's prediction accuracy. More importantly, results indicate that the emission reduction amount, MW per hour, investment/Emission as crucial variables, whereas the baseline methodology and technology types were insignificant. This demonstrates that at least for hydro power projects, the specific technology is not as important as the amount of emission reductions and relatively small scale projects and investment to carbon reduction ratios.

• The Korea Journal of Herbology
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• v.25 no.1
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• pp.75-81
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• 2010

Objectives : It was investigated new herbal prescription HT005 supported by traditional Korean medicine has a activity on the longitudinal bone growth of rats. HT005 were consisted of the root of Angelica sinensis, the fruit of Alpinia oxyphylla, the root of Astragalus membranaceus, the stem of Eleutherococcus senticosus, and the root of Dioscorea japonica, and Poria cocos. Method : To investigate the effect on longitudinal bone growth in adolescent male Sprague-Dawley rats, the longitudinal length of growth plate was measured directly by the tetracycline fluorescent labeling method and chondrocyte staining method. HT005 administered orally for four days, and the tetracycline was injected twice on the growth plate of the animals for fluorescent dying. The rate of longitudinal bone growth was measured the length between the tetracycline bands which fixed on the 3rd day and 5th day after injection. Cresyl violet was also used to stain the chondrocytes in the growth plate. The length of growth plate after administration was compared. Expression of IGF-1 and BMP-2 in the growth plate was investigated by immunohistochemistry. Results : HT005 showed a significant longitudinal bone growth which was $301.0{\pm}6.1\;{\mu}m/day$ at the dose of 100 mg/kg and $283.8{\pm}1.25\;{\mu}m/day$ (p < 0.001)at the dose of 10 mg/kg of HT005, compared to control group by the tetracycline fluorescent labeling method. HT005 showed a significant chondrocyte length on growth plate which was measured $797.19{\pm}3.31\;{\mu}m$ (p < 0.001) at the dose of 100 mg/kg and $720.14{\pm}2.19$ (p < 0.001) ${\mu}m$ at the dose of 10 mg/kg compared to the control group by cresyl-violet staining method. Both the number and intensity of BMP-2 and IGF-1 positive cells were increased in the hypertrophic zone of growth plate. There was a significant correlation between BMP-2 and IGF-1 expression and heights of chondrocytes in growth plate. Conclusions : Treatment of HT005 on Sprague-Dawley rats markedly increased the longitudinal bone growth. Therefore, HT005 may be an alternative herbal source to growth hormone as it promotes bone growth in children afflicted by growth retardation.

• Journal of the Korea Society of Computer and Information
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• v.5 no.2
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• pp.30-36
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• 2000

The hidden node plays a role of the functional units that classifies the features of input pattern in the given question. Therefore, a neural network that consists of the number of a suitable optimum hidden node has be on the rise as a factor that has an important effect upon a result. However there is a problem that decides the number of hidden nodes based on back-propagation learning algorithm. If the number of hidden nodes is designated very small perfect learning is not done because the input pattern given cannot be classified enough. On the other hand, if designated a lot, overfitting occurs due to the unnecessary execution of operation and extravagance of memory point. So, the recognition rate is been law and the generality is fallen. Therefore, this paper suggests a method that decides the number of neural network node with feature information consisted of the parameter of learning algorithm. It excludes a node in the Pruning target, that has a maximum value among the feature value obtained and compares the average of the rest of hidden node feature value with the feature value of each hidden node, and then would like to improve the learning speed of neural network deciding the optimum structure of the multi-layer neural network as pruning the hidden node that has the feature value smaller than the average.

• The Journal of the Acoustical Society of Korea
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• v.39 no.5
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• pp.497-504
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• 2020

Constructing speaker embeddings in speaker verification is an important issue. In general, a self-attention mechanism has been applied for speaker embedding encoding. Previous studies focused on training the self-attention in a high-level layer, such as the last pooling layer. In this case, the effect of low-level layers is not well represented in the speaker embedding encoding. In this study, we propose Masked Cross Self-Attentive Encoding (MCSAE) using ResNet. It focuses on training the features of both high-level and low-level layers. Based on multi-layer aggregation, the output features of each residual layer are used for the MCSAE. In the MCSAE, the interdependence of each input features is trained by cross self-attention module. A random masking regularization module is also applied to prevent overfitting problem. The MCSAE enhances the weight of frames representing the speaker information. Then, the output features are concatenated and encoded in the speaker embedding. Therefore, a more informative speaker embedding is encoded by using the MCSAE. The experimental results showed an equal error rate of 2.63 % using the VoxCeleb1 evaluation dataset. It improved performance compared with the previous self-attentive encoding and state-of-the-art methods.