Korean Journal of Clinical Laboratory Science (대한임상검사과학회지)

Korean Society for Clinical Laboratory Science (대한임상검사과학회)
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Antimicrobial Activity of Houttuynia cordata Ethanol Extract against Major Clinical Resistant Microorganisms

주요 임상 내성균에 대한 어성초 에탄올 추출물의 항균효과

  • Received : 2015.08.02
  • Accepted : 2015.08.21
  • Published : 2015.09.30
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Abstract

The increase in resistance by pathogenic bacteria to multiple antimicrobial agents has become a significant treat, as the effective antimicrobial agents available for the patients infected by such resistant bacteria are reduced, or even eliminated. Several natural plant extracts have exhibited antibacterial and synergistic activity against various resistant microorganisms. Houttuynia cordata is frequently used by many traditional medicine practicioners for its antimicrobial, antiviral, and anti-inflammatory properties. This study investigated the antibacterial effects of H. cordata extract against clinical multi-resistant bacteria, and compared the two methods used for the antimicrobial susceptibility testing. Thirty isolates of Methicillin-resistant Staphylococcus aureus (MRSA, 10), Vancomycin-resistant Enterococcus faecium (VRE, 10), Carbapenem-resistant Acinetobacter baumannii (CRAB, 10) were included in this study. The antibacterial effect of H. cordata was tested by disk diffusion and microbroth dilution methods as per CLSI guidelines. In disk diffusion, all isolates (30) showed no inhibition to 30,000 ug/mL of H. cordata. But in the microbroth dilution method, $MIC_{90}$ of H. cordata was 4,096 ug/mL, 8,192 ug/mL and 4,096 ug/mL in MRSA, VRE and CRAB, respectively. These results demonstrate that H. cordata exhibits antibacterial activity against MRSA, VRE and CRAB. Moreover, the microbroth dilution method is a more effective method than disk diffusion to evaluate the antibacterial activity of natural products. The Disk diffusion method used to evaluate the antibacterial activity of natural products required new standard guidelines including inoculum concentration of bacteria.

다제내성균의 증가는 사용할 항생제가 적거나 심지어 선택할 항생제가 없기 때문에 감염환자의 치료에 커다란 위협이 되고 있다. 최근 다양한 천연 추출물이 내성세균에서 항균효과 및 상승효과를 보인다는 보고가 많다. 어성초(Houttuynia cordata)는 항균, 항바이러스 및 항산화 효과를 보이는 전통적 약초이다. 이에 저자들은 임상의 주요 내성균에 대한 어성초의 항균효과를 알아보고, 이들의 적절한 항균효과 측정방법을 평가하고자 한다. 우선 현재 가장 많은 문제를 야기하는 주요 내성균인 methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant Enterococcus faecium (VRE), carbepenem-resistant Acinetobacter baumannii(CRAB) 각각 10 균주를 선택하여, CLSI 기준으로 디스크 확산법 및 미량액체배지희석법으로 감수성 시험을 하였다. CLSI 기준으로 시행한 디스크 확산법에서 $4,096{\mu}g/mL$에서 $30,000{\mu}g/mL$ 까지의 농도에서 모든 균의 억제대는 관찰되지 않았다. 그러나 액체배지 희석법에서는 MRSA, VRE, CRAB의 $MIC_{90}$이 각각 $4,096{\mu}g/mL$, $8,192{\mu}g/mL$, $4,096{\mu}g/mL$를 보였다. 결론적으로 어성초는 MRSA, VRE, CRAB 등 주요 임상 내성균에서 우수한 항균효과를 보이며 천연물의 항균효과를 평가에 디스크 확산법보다 액체배지 희석법이 유용하다고 할 수 있다. 만약 디스크 확산법으로 항균효과를 측정하려면 천연물에 대한 균 접종 농도의 변경 등 새로운 기준이 요구된다.

Keywords

References

  1. Amin MU, Khurram M, Khattak B, Khan J. Antibiotic additive and synergistic action of rutin, morin and quercetin against methicillin resistant Staphylococcus aureus. BMC Complement Altern Med. 2015, 15:59-72. https://doi.org/10.1186/s12906-015-0580-0
  2. Brakstad OG, Aasbakk K, Maeland JA. Detection of Staphylococcus aureus by polymerase chain reaction amplication of the nuc gene. J Clin Microbiol.1992, 30: 1654-60.
  3. Chin JN, Jones RN, Sader HS, Savage PB, Rybak MJ. Potential synergy activity of the novel ceragenin, CSA-13, against clinical isolates of Pseudomonas aeruginosa, including multidrug- resistant P. aeruginosa. J Antimicrob Chemother. 2008, 61:365-370.
  4. Cho WC, Kim JI, Kim MJ. Microbiological effects of xanthorrhizol and Houttuynia cordata Thunb. extract. J Korean Oil Chemists'Soc. 2008, 25:269-274.
  5. Chung HS, Lee YS, Park ES, Lee DS, Ha EJ, Kim MS, et al. Characterization of the multidrug-resistant Acinetobacter species causing a nosocomial outbreak at intensive care units in a Korean teaching hospital. Ann Clin Microbiol. 2014, 17:29-34. https://doi.org/10.5145/ACM.2014.17.2.29
  6. Clinical Laboratory Standards Institute. 2012. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Approved standard (M07-A9), CLSI. Wayne, PA.
  7. Depardieu F, Perichon B, Courvalin P. Detection of the van alphabet and identification of Enterococci and Staphylococci at the species level by multiplex PCR. J Clin Microbiol.2004, 42:5857-5860. https://doi.org/10.1128/JCM.42.12.5857-5860.2004
  8. Hong SB, Yum JH, Kim YY, Shin KS. Trends in pathogen occurrence and antimicrobial resistance of urinary isolates in a tertiary medical center over ten years:2004-2013. Biomedical Science Letters. 2015, 21:84-91. https://doi.org/10.15616/BSL.2015.21.2.84
  9. Hong SB. 2013 Academic research Report of the Korean association of quality assurance for clinical laboratory. Korean Assoc Qual Assur Clin Lab. 2013, 7-9.
  10. Jang MJ, Kim YH, An BJ, Lee CE, Lee JT, Kim SH, et al. Study on anti-inflammatory and anti-microbial effect of Pinusrigida Mill. Inner bark extracts as a cosmetic material. J Korean For Soc. 2008, 97:215-220.
  11. Jeong HR, Kwak JH, Kim JH, Choi GN, Jeong CH, Heo HJ. Antioxidant and neuronal cell protective effects of an extract of Houttuynia cordata Thunb. Korean J Food Preserv. 2010, 17:720-726.
  12. Jo SH, Cho CY, Ha KS, Choi EJ, Kang YR, Kwon YI. The antioxidant and antimicrobial activities of extracts of selected barley and wheat inhabited in Korean Peninsula. J Korean Soc Food Sci Nutr. 2013, 42:1003-1007. https://doi.org/10.3746/jkfn.2013.42.7.1003
  13. Jorgensen JH, Turnidge JD. Susceptibility test methods; dilution and disk diffusion methods. In: Manual of clinical microbiology. 9th ed, 2007, p1152-1172. ASM press, Washington, D.C.
  14. Kim CH, Lee JY, Kim MK, Kim SH, Park GY, Bae SY, et al. Isolation rate of methicillin-resistant Staphphylococcus aureus (MRSA) from nasal cavity inferior regions and cellular phones. Korean J Clin Lab Sci. 2012, 44:118-123.
  15. Kim DH, Lim JJ, Lee JJ, Jung WC, Shin HJ, Lee HJ, et al. Antibacterial and therapeutic effects of Houttuynia cordata ethanol extract for murine Salmonellosis. Korean J Environ Agric. 2008, 27:156-162. https://doi.org/10.5338/KJEA.2008.27.2.156
  16. Kim SK, Ryu SY, Choi SU, Kim YS. Cytotoxic alkaloids from Houttuynia cordata. Arch Pharm Res. 2001, 24:518-521. https://doi.org/10.1007/BF02975156
  17. Kim WJ, Cho JY, Choi CS, Yoon GS, Lee WK, Ryu YW. Antimicrobial activity of extracted by supercritical fluid from Origanumvulgare, Cinnamomum cassia, Chamaecyparisobtuse and Scutellariaebaicalensis. Korean J Biotechnol Bioeng. 2008, 23:147-152.
  18. Lee JY, Bae HJ, Park TS, Kim TY, Moon DH, Kwon OJ, et al. Anti-oxidant and anti-microbial activities of Seungma galgeuntang. J Appl Biol Chem. 2010, 53:13-20. https://doi.org/10.3839/jabc.2010.003
  19. Lee WW, Lee SM, Lee GR, Kim GH. Antimicrobial effects of galic extract against pathogenic bacteria. The annual report of Busan metropolitan city institution of health & environment. 2011, 20:231-246.
  20. Liu IX, DG Durham, RM Richards. Baicalin synergy with $\beta$-lactam antibiotics against methicillin-resistant Staphylococcus sureus other $\beta$-Lactam-resistant strains of S. aureus. J Pharm Pharmacol. 2000, 52: 361-366. https://doi.org/10.1211/0022357001773922
  21. Lu H, Wu X, Liang Y, Zhang J. Variation in chemical composition and ntibacterial ctivities of essential oils from two species of Houttuynia Thunb. Chem Pharm Bull. 2006, 54:936-940. https://doi.org/10.1248/cpb.54.936
  22. Lu X, Yang X, Li X, Lu Y, Ren Z, Zhao L. et al. In vitro activity of sodium new Houttuyfonate alone and in combination with oxacillin or netilmicin against methicillin-resistant Staphylococcus aureus. PLOS one. 2013, 8;e68053-e68060. https://doi.org/10.1371/journal.pone.0068053
  23. Mahady GB. Medicinal plants for the prevention and treatment of bacterial infections. Curr Pharm Des. 2005, 11:2405-2427. https://doi.org/10.2174/1381612054367481
  24. Park SO, Park BS, Ryu CM, Ahn YS. Effect of herb extracts mixed with Houttuynia cordata on antiatopic dermatitis in DNCB-induced BALB/c mouse. J Korean Oil Chemists'Soc. 2012, 29: 175-183.
  25. Shin SH, Sung IH. Antimicrobial activities of the extracts from Paeonia japonica against MRSA. Korean J Microbiol. 2006, 42:54-58.
  26. Song JH, Kim MJ, Kwon HD, Park IH. Antimicrobial activity of fractional eatracts of Houttuynia Cordara root. J Korean Soc Food Nutr. 2003, 32: 1053-1058. https://doi.org/10.3746/jkfn.2003.32.7.1053
  27. Woodford, N., Ellington, M. J., Turton, J. F., Ward, M. E., Brown, S.,Amyes, S. G. & Livermore, D. M. Multiplex PCR for genes encording prevalent OXA carbapenemases in Acinetobacter spp.. Int J Antimicrob Agents. 2006, 27:351-353. https://doi.org/10.1016/j.ijantimicag.2006.01.004
  28. Yang BS, Park JY, Choi SG. Rapid detection of vancomycin-resistant Enterococci (VRE) in clinical samples from university hospital. Korean J Clin Lab Sci. 2013, 45:16-20.

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