• Animal cells and systems
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• v.3 no.3
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• pp.253-258
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• 1999

The present study describes histochemical properties of the mucosubstances in the pedal sole of abalone, in relation to two main additional functions known to date. Only neutral mucosubstances were demonstrated in the lateral epithelium of the pedal sole of the abalone, however, two distinct types of cells, one containing neutral and the other containing acidic mucosubstances, were identified in the ventral epithelium. The epithelial mucocytes distributed throughout both epithelial and subepithelial glands located in the ventral subepithelial region showed a mixture of neutral and acidic mucosubstances. However, acidic mucosubstances were found in larger amounts than neutral ones in epithelial mucocytes, and vice versa in subepithelial glands. Among these acidic mucosubstances, sulphated substances were dominant. Lesser amounts of carboxylated substances also occurred together with low levels of neuramic and sialic acid. Collectively, the notable morphological differences between abalone and other gastropoda were observed in the ventral surface of the foot, which showed a tall columnar epithelium, peripherally concentrated mucocytes, and subepithelial glands. Mucosubstances are presumably produced by these cells and may also play a role in clinging to some substances to support abalones in addition to the main functions of lubrication and protection.

• Korean Journal of Clinical Laboratory Science
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• v.36 no.2
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• pp.173-177
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• 2004

This study is performed in order to clarify the histochemical structure, the distribution of mucous cell and goblet cell, and the histochemical properties of the mucosubstances in the middle region of intestinal mucosa and rectum of Raja kenojei. In the H&E stain, distribution of the mucous cells and acidophilic cells were a more compacted middle portion than other regions of intestine, but the former was more than the latter in the number of mucous cells to rectum. The mucosubstances were stained with aldehyde fuchsin pH 1.7-alcian blue(pH 2.5) stain and then compared to distribution of the mucosubstances used in image and microscope technology(IMT-Size5). The middle intestine of Raja kenojei was composed of mucous cells having only large amounts of mucosubstances in the distal region was much more than that of proximal region. It was two types of mucous cells to rectum, one type was the same as proximal intestine while the other had small amounts of weakly sulfated and large amounts of carboxylated mucins.

• Biomolecules & Therapeutics
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• v.9 no.4
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• pp.263-269
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• 2001

In this study, we tried to investigate whether poly-L-arginine (PLA) (MW 10,800) significantly affect mucin release from cultured hamster airway goblet cells and the mucosubstances of hypersecretory air-way goblet cells of rats. Confluent primary hamster tracheal surface epithelial (HTSE) cells were metabolically radiolabeled with $^3$H-glucosamine for 24 hr and chased for 30 min in the presence of varying concentrations of PLA to assess the effects on $^3$H-mucin release. Possible cytotoxicities of PLA were assessed by measuring both Lactate Dehydrogenate (LDH) release and by checking the possible changes on the morphology of HTSE cells during treatment. For in vivo experiment, hyperplasia of rat airway goblet cells and increase in intraepithelial mucosubstances were induced by exposing rats to SO$_2$ for 3 weeks and varying concentrations of PLA were administered inhalationally to assess the effects on the mucosubstances of airway goblet cells of rats. The results were as follows : (1) PLA significantly inhibited mucin release from cultured HTSE cells in a dose-dependent manner; (2) there was no significant release of LDH and no significant change on the morphology of cultured HTSE cells during treatment; (3) PLA also affected the intraepithelial mucosubstances of hypersecretory rats and restored them to the levels of control animals. We conclude that PLA inhibit mucin release from airway goblet cells without significant cytotoxicity and possibly normalize the hypersecretion of airway mucosubstances in vivo. This finding suggests that PLA might function as an airway mucoregulative agent.

• The Korean Journal of Zoology
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• v.17 no.1
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• pp.43-50
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• 1974

This study was performed in order to know the histochemical changes of mucosubstances in mucous glands under normal and dry conditions. The frogs, Rana nigromaculata Hallowell, were collected near Seoul area in mid-August. The experimental group was retained under dry conditions for 12, 24, 30 and 36 hrs to make a comparison to normal group. The results obtained were as follows: 1. Mucosubstances in mucous glands of the frog skin contain neutral mucin and sulfated and non-sulfated mucins. Mucosubstances in the cells of the gland neck are neutral mucin, while the gland body is composed of neutral, sulfated and non-sulfated mucins. 2. Mucosubstances in mucous gland of the frog skin decreased distinctively under dry conditions, as is evident from the hitochemical properties, te neutral mucins were influenced more clearly than acid mucins. 3. Histochemical compositions of the mucosubstances in the cell of the mucous gland (the gland neck and the gland body) were not changed. 4. The phenomena were considered as a general adaptation syndrome to keep homeostasis.

• The Korean Journal of Zoology
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• v.18 no.3
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• pp.107-114
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• 1975

This study was performed in order to know the histochemical changes of mucosubstances in granular secretions of the serous gland under normal and dry conditions. The frogs were collected near Jinju and the experimental groups were retained under dry conditions for 24, 48, 72 and 96 hours to make a comparison with normal group. The results obtained were as follows: 1. The secreting granules in serous gland of the frog skin contained neutral and weakly acidic mucosubstances. 2. the secreting granules in serous gland of the frog skin were significantly decreased under dry conditions, as is evident from the histochemical properties. The neutral mucosubstances were decreased more than acidic mucosubstances. 3. Histochemical compositions of the mucosubstances of secreting granules in the lumen of the serous gland were not changed according to dry condition.

• Korean Journal of Poultry Science
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• v.29 no.3
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• pp.177-183
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• 2002

The study was designed to investigate the aspects of the goblet cell of the oviduct in laying Korean ring-necked pheasants by histochemical methods (PAS, alcian blue-PAS, alcian blue pH 2.5, alcian blue pH 1.0, and aldehyde fuchsin-alcian blue stain). Twelve laying Korean ring-necked pheasants were used. The results were summarized as follows. The goblet cell of the oviduct of the Korean ring-necked pheasant contained sulfated acid mucosubstances and neutral mucosubstances in the infundibulum. The compounds of sulfated acid mucosubstances, nonsulfated acid mucosubstances, and neutral mucosubstances are present in goblet cells of the magnum, vagina and openings of the tubular gland of the uterus of the oviduct in the Korean ring-necked pheasant. The goblet cell of the isthmus and uterus of the oviduct of the Korean ring-necked pheasant contained neutral mucosubstances except openings of the tubular gland of the uterus. The histochemical characteristics of secretory granules of goblet cells in oviduct of the Korean ring-necked pheasant were not related to the position of eggs in oviduct.

• The Korean Journal of Zoology
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• v.17 no.2
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• pp.69-80
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• 1974

The mucosubstances in the gastrointestinal mucous secreting cells of Rana rugosa were detected histochmeically during pre-hibernating, hibernating, post-hibernating and active phases. The results of the observation were as follows: 1. The mucosubstances in the gastrointestinal mucous secreting cells of active frog were strongly PAS-active in stomach, PAS-active and alcianophilic at pH 2.5 in small intestine and alcianophilic at pH 2.5 in large intestine. 2. The PAS-active mucosubstances in the gastric surface epithelial cells were increased remarkably during hibernation. 3. The alcianophilic mucosubstances at both pH 2.5 and pH 1.0 were decreased remarkably in the goblet cells of small intestine during hibernation, but a little PAS-active ones were increased. 4. The alcianophilic mucosubstances at pH 2.5 were decreased remarkably and a lttle PAS-active ones also were done in the goblet cells of large intestine during hibernation. 5. The increases of the contained quantity of mucosubstances in the gastric surface epithelial cells during hibernationi may have theeffects of preventing cohesions of gastric mucosae and suppressing activities of gastric acid and enzymes. The mucosubstances neutral acidity in the intestine during hibernation may be secreted, because of acidity being done near neutrality in its lumen, due to remarkable decrease of intestinal juices and gastric acids.

• Animal cells and systems
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• v.12 no.2
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• pp.85-91
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• 2008

Structure and mucosubstance histochemistry in the epidermis of five teleostean species, i. e., spotty belly, Agrammus agrammus, devil stinger, Inimicus japonicus, stone fish, Erosa erosa, cubed snailfish, Liparis tessellatus, and Japanese bluefish, Scombrops boops were investigated. The epidermis of five species studied is composed of three layers: superficial, middle, and basal layer. The superficial layer is comprised of rather flattened cells. Mucous cells, the type commonly found in fishes are completely lacking in the epidermis of devil stinger, stone fish, and cubed snailfish. The epidermis of devil stinger and stone fish have multicellular glands which do not have mucosubstances. The skin surface of them is covered with mucous layer. The superficial cells in the epidermis of devil stinger, stone fish, and cubed snailfish are mucus-secreting cells. The composition and the amount of the mucosubstances vary in species and body regions. The mucous layer on the skin surface and superficial epidermal cells of devil stinger contain a mixture of neutral and acidic(sulfated and nonsulfated) mucosubstances. In stone fish, the mucous layer has acidic(sulfated and non-sulfated) mucin and the superficial epidermal cells contain neutral mucin. In cubed snailfish, the type of epidermal mucosubstances is identified as a mixture of neutral and acidic(non-sulfated) mucin. The mucous cells of the epidermis in spotty belly and Japanese bluefish contain neutral mucin.

• Journal of Life Science
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• v.15 no.5 s.72
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• pp.707-714
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• 2005

The conventional histochemical staining were used to study mucosubstances properties of intestinal striated border and goblet cells in four teleostean species, i. e., Sebastes schlegeli, Halichoeres poecilopterus, Bryzoichthys lysimus, and Takifugu pardalis. The following methods were used; PAS, AB pH 2.5, AB pH 1.0, AB pH 2.5-PAS, AF pH 1.7-AB pH 2.5 and HID-AB pH 2.5 stains. The mucosubstances of striated border in the proximal intestine and rectum of Sebastes schlegeli contained with neutral mucin, middle and distal intestine contained with neutral mucin and acid mucin. The striated border of all the intestines of Halichoeres poecilopterus contained with neutral mucin and acid mucin, and those of Bryzoichthys lysimus and Tnkifugu pardalis contained with neutral mucin only. The amounts of neutral mucin were moderate to considerable in Sebastes schlegeli and Halichoeres poecilopterus, minimal to small in Bryzoichthys lysimus and Tnkifugu pardalis. The amounts and properties in mucosubstances of intestinal goblet cells showed differences in species and regions. The intestinal goblet cells of Bryzoichthys lysimus, and Tnkifugu pardalis contained neutral mucin only while Sehastes schlegeli and Halichoeres poecilopterus contained mixture of neutral mucin, sulfomucin and sialomucin. The amounts of neutral mucin were considerable to large in distal intestine and rectum of Tnkifugu pardalis, while moderate to considerable in all intestines of Sehastes schlegeli, all the intestines except for middle intestine of Bryzoichthys lysimus, and proximal and middle intestine of Tnkifugu pardalis. Also it was minimal to small in middle intestine of Halichoeres poecilopterus. The intestinal goblet cells of Sehastes schlegeli contained mixture of minimal amounts of strong sulfmucin, weak sulfomucin and minimal to small amounts of sialomucin, and those of Halichoeres poecilopterus except for rectum contained mixture of minimal to small amounts of strong sulfomucin and sialomucin.

• Proceedings of the Zoological Society Korea Conference
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• 1994.10a
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• pp.151.2-151
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• 1994

No Abstract, See Full Text