Animal cells and systems

The Korean Society for Integrative Biology (한국통합생물학회)
권호 표지

A Study on the Epidermal Structure and Mucosubstance Histochemistry of Spotty Belly, Devil Stinger, Stone Fish, Cubed Snailfish, and Japanese Bluefish

  • Jeong, Gil-Nam (Biology Education Major, Division of Science Education, College of Education, Pusan National University)
  • Published : 2008.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Structure and mucosubstance histochemistry in the epidermis of five teleostean species, i. e., spotty belly, Agrammus agrammus, devil stinger, Inimicus japonicus, stone fish, Erosa erosa, cubed snailfish, Liparis tessellatus, and Japanese bluefish, Scombrops boops were investigated. The epidermis of five species studied is composed of three layers: superficial, middle, and basal layer. The superficial layer is comprised of rather flattened cells. Mucous cells, the type commonly found in fishes are completely lacking in the epidermis of devil stinger, stone fish, and cubed snailfish. The epidermis of devil stinger and stone fish have multicellular glands which do not have mucosubstances. The skin surface of them is covered with mucous layer. The superficial cells in the epidermis of devil stinger, stone fish, and cubed snailfish are mucus-secreting cells. The composition and the amount of the mucosubstances vary in species and body regions. The mucous layer on the skin surface and superficial epidermal cells of devil stinger contain a mixture of neutral and acidic(sulfated and nonsulfated) mucosubstances. In stone fish, the mucous layer has acidic(sulfated and non-sulfated) mucin and the superficial epidermal cells contain neutral mucin. In cubed snailfish, the type of epidermal mucosubstances is identified as a mixture of neutral and acidic(non-sulfated) mucin. The mucous cells of the epidermis in spotty belly and Japanese bluefish contain neutral mucin.

Keywords

References

  1. Alexander JB and Ingram GM (1992) Noncellular nonspecific defense mechanisms of fish. Annu Rev Fish Dis 3: 249-279
  2. Cole AM, Weis P, and Diamond G (1997) Isolation and characterization of pleurocidin, and antimicrobial peptide in the skin secretions of winter flounder. J Biol Chem 272: 1208-1213
  3. Fletcher TC (1982) Non-specific defence mechanisms in fish. Developmental and Comparative Immunology 2(Suppl) 123
  4. Handy RD, Eddy FB, and Roman G (1989) In vitro evidence for the ionoregulatory role of rainbow trout mucus in acid, acid/ aluminium and zinc toxicity. J Fish Biol 35: 737-747 https://doi.org/10.1111/j.1095-8649.1989.tb03024.x
  5. Harrell LW, Etlinger HM, and Hodgins HO (1976) Humoral factors important in resistances of salmonid fish to bacterial disease. II. Anti Vibrio anguillarum activity in the mucus and observations on complement. Aquaculture 7: 363-370 https://doi.org/10.1016/0044-8486(76)90133-2
  6. Jeong GN and Jo UB (2007) Mucosubstance histochemistry of the epidermis in yellowtail, striped beakperch, brown spotted grouper, sea chub, and multicolorfin rainbowfish. Integ Biosci 11: 183-189
  7. Kazuyori Y and Motoyoshi Y (1975) Morphochemical analysis of mucosubstances in some epithelial tissues of the eel (Anguilla japonica). Histochemistry 43: 161-172 https://doi.org/10.1007/BF00492444
  8. Kim JW, Chin P, Jin YG, and Lee JS (2002) Ultrastructure on the integumentary epidermis of the bastard halibut, Paralichthys olivaceus (Teleostei: Pleuronectidae). Korean J Electron Microscopy 32(2): 121-129. (in Korean)
  9. Lee JS and Kim JW (1999) Fine structure of the integumentary supporting cell and gland cell of the sea bass, Lateolabrax japonicus (Teleostei: Moronidae). Korean J Electron Microscopy 29(4): 417-426. (in Korean)
  10. Lee JS, An CM, and Huh SH (2000) Integumentary ultrastructure of the blenny, Pholis nebulosa (Teleostei: Pholidae). J Korean Fish Soc 33: 148-152
  11. Lev R and Spicer SS (1964) Specific staining of sulfated group with alcian blue at low pH. J Histochem Cytochem 12: 309 https://doi.org/10.1177/12.4.309
  12. McManus JFA (1968) Periodic acid Schiff's reaction. Stain Techn 23: 99-108, 1948(AFIP modification). Copyright by manual of histologic staining methods of the armed forces
  13. Mittal AK and Banerjee TK (1974) Structure and keratinization of the skin of a fresh-water teleost Notopterus notopterus. J Zool Lond 174: 341-355 https://doi.org/10.1111/j.1469-7998.1974.tb03162.x
  14. Mittal AK and Munshi JSD (1971) A comparative study of the structure of the skin of certain air-breathing fresh-water teleosts. J Zool Lond 163: 515-532 https://doi.org/10.1111/j.1469-7998.1971.tb04547.x
  15. Mittal AK, Ueda T, Fujimori O, and Yamada K (1994) Histochemical analysis of glycoproteins in the epidermal mucous cells and sacciform cells of an Indian swamp eel, Monopterus cuchia (Hamilton) (Synbranchiformes, Pisces). Acta Histochem Cytochem 27(3): 193-204 https://doi.org/10.1267/ahc.27.193
  16. Mittal AK, Whitear M, and Agarwal SK (1980) Fine structure and histochemistry of the epidermis of the fish, Monopterus cuchia. J Zool Lond 191: 107-125 https://doi.org/10.1111/j.1469-7998.1980.tb01452.x
  17. Mowry RW (1963) The special value or method that color both acidic and vicinal hydrate groups in the histochemical study of mucin. Ann NY Acad Sci 106: 402-423 https://doi.org/10.1111/j.1749-6632.1963.tb16654.x
  18. Olafsen JA (1995) Bacterial antigen priming of marine fish larvae. Advances in Experimental Medicine and Biology 371A: 349-352
  19. Park JY and Kim CH (2007) Habitats and air uptake based on analysis of skin structure of two Korean bullheads, Pseudobagrus brevicorpus and P. koreanus (Pisces; Bagridae). Integ Biosci 11: 155-160
  20. Park JY and Kim IS (2000) Structure and cytochemistry of skin in spined loach, Iksookimia longicorpus (Pisces, Cobitidae). Korean J Ichthyol 12: 25-32
  21. Park JY, Kim IS, and Kim SY (2001) Morphology and histochemistry of the skin of the mud loach, Misgurnus mizolepis, in relation to cutaneous respiration. Korean J Biol Sci 5: 303-308 https://doi.org/10.1080/12265071.2001.9647619
  22. Park JY, Kim IS, Lee YJ, and Baek HA (2005) Mucous cells and their structure on the epidermis of five appendages in the Korean flat-headed goby, Luciogobius guttatus (Pisces; Perciformes). Korean J Ichthyol 17(3): 167-172
  23. Pickering AD and Macey DJ (1977) Structure, histochemistry and the effect of handling in the mucous cells of the epidermis of the char Salvelinus alpinus (L.). J Fish Biol 10: 505-512 https://doi.org/10.1111/j.1095-8649.1977.tb04083.x
  24. Pickering AD and Richards RH (1980) Factors influencing the structure, function and biota of the salmonid epidermis. Proceeding of the Royal Society of Edinburgh 79B: 93-104
  25. Rosen MW and Conford NE (1971) Fluid friction of fish slime. Nature 234: 49-51 https://doi.org/10.1038/234049a0
  26. Shephard KL (1994) Functions for fish mucus. Rev Fish Biol Fish 4(4): 401-429 https://doi.org/10.1007/BF00042888
  27. Singh SK and Mittal AK (1990) A comparative study of the epidermis of the common carp and the three Indian major carp. J Fish Biol 36: 9-19 https://doi.org/10.1111/j.1095-8649.1990.tb03515.x
  28. Spicer SS and Meyer DB (1960) Histochemical differentiation of acid mucopolysaccharides by means of combined aldehyde fuchsin-alcian blue staining. Am J Clin Pathol 33: 454-460
  29. Spicer SS, Horn RG, and Leppi TJ (1967) Histochemistry of connective tissue mucopolysaccharides. In: Wagner BM and Smith DE (eds), The connective tissue, Williams & Wilkins, Baltimore, pp 251-303
  30. Whitear M and Mittal AK (1984) Surface secretions of the skin of Blennius (Lipophrys) pholis L. J Fish Biol 25: 317-331 https://doi.org/10.1111/j.1095-8649.1984.tb04879.x
  31. Zhang J, Taniguchi T, Takita T, and Ali AB (2003) A study on the epidermal structure of Periophthalmodon and Periophthalmus mudskippers with reference to their terrestrial adaptation. Ichthyol Res 50: 310-317 https://doi.org/10.1007/s10228-003-0173-7