• Title/Summary/Keyword: MsrB

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Investigation into Whether Agronomic Traits Are Fixed for the Breeding of Drought Tolerance and BPH-resistant Crosses (내건성 및 BPH 내성 계통의 육성을 위한 농업형질 고정여부 조사)

  • Lee, So Young;Kim, Eun-Gyeong;Park, Jae-Ryoung;Kim, Kyung-Min
    • Journal of Life Science
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    • v.30 no.9
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    • pp.798-803
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    • 2020
  • Late in the 1960s, South Korea established cultivation technology and began breeding new "Tongil" type rice cultivars by crossing indica and japonica. To date, this cultivation technique has been used to produce a wide variety of cultivars to meet consumer preferences and adapt to extreme weather conditions. Once major genetic traits are no longer segregated through advanced generations, varieties obtained from a cross between different crops may become a new variety. In this study, we confirmed the agronomic traits of F4, F5, and F6 by advanced generation a population in which drought tolerance and brown planthopper (BPH) resistance were crossed. HV23, Ilmi/HV23, and Drimi2ho/HV23 were used. HV23 was drought-tolerant, and Drimi2ho was resistant to BPH. As a result, it was possible to consider that the agronomic traits were fixed because none of them showed a significant difference from the others. While it takes more than 10 years for the pedigree method of breeding, this study confirmed that the agronomic traits were fixed in 4-6 years. In the future, we will investigate the homology of the CaMsrB2 gene and the Bph1 gene, to confirm that both genes are closely related to each other, and analyze the stable inheritance of the introduced gene for multiple successive generations.

Antibiotic Resistance and Safety Assessment of Enterococcus faecium CKDB003 for Using as Probiotics (프로바이오틱스 Enterococcus faecium CKDB003의 항생제 내성 및 안전성 평가)

  • Kim, Han Jun;Kang, Soon Ah
    • The Korean Journal of Food And Nutrition
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    • v.33 no.3
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    • pp.223-236
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    • 2020
  • In this study, a safety evaluation was conducted to confirm if the Enterococcus faecium CKDB003 strain obtained by selection from a mixed fermentation of fruit and milk is suitable for use as a probiotic. The MIC value for the 10 antibiotics specified in the EFSA guidance was below the acceptable cut-off value. The antibiotic resistance genes aac(6')-li, eatAv, and msr(C) exist by whole genome sequencing, but are in the chromosome and not in the plasmid, thus confirming that there is no possibility of transmission to other microorganisms. It was confirmed that cytolysin (cylA, cylB, cylI, cylL-l, cylL-s, cylM, cylR1, cylR2), aggregation substance (asa1, asp1), collagen adhesion (ace), enterococcal surface protein (esp), endocarditis antigen (efaA), hyaluronidase (hyl) and gelatinase (gelE) were not present in the genome by examining the genes of factors related to virulence. Also, the biochemical analysis showed no toxic enzyme activities, and no virulence genes were detected by the PCR method. Thus, the E. faecium CKDB003 strain can be safely used as a health functional food probiotic, based on the results of the safety assessment.

Finite Element Analysis and Measurement on the Release of Residual Stress and Non-linear Behavior in Weldments by Mechanical Loading(I) -Experimental Examination-

  • Jang, K.B.;Yoon, H.S.;Cho, S.M.
    • International Journal of Korean Welding Society
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    • v.2 no.1
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    • pp.40-44
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    • 2002
  • Residual stress by welding should be reduced because that decreases the reliability on strength of welded structure. The reason is that the total stiffness of structure decreases by non-linear behavior of weldment under external load. The release of residual stress by mechanical loading and unloading is often performed in the fabrication of box structure for steel bridge. The proper degree of loading and unloading is significant at release method of residual stress by mechanical loading because that degree is changed by material and geometric shape of welded structure. Therefore, the simulation model that could exactly analyze the release of residual stress by mechanical loading is to be necessary. This simulation model should be established on the based of variable and accurate measurement data. In this study, the non-linear behavior of weldments under external loading and unloading, such as the decrease and increase of structure stiffness, was investigated by monitoring of nominal stress and strain. Tensile loading and unloading test under variable load was performed and the proper degree of stress relaxation was measured by sectioning technique using strain gauge.

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Physiological Responses of Warm-Season Turfgrasses under Deficit Irrigation (소량관수로 인한 난지형 잔디의 생리적 반응)

  • Lee, Joon-Hee;Trenholm, Laurie. E.;Unruh, J. Bryan
    • Asian Journal of Turfgrass Science
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    • v.23 no.1
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    • pp.9-22
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    • 2009
  • Due to increasing concerns over issues with both water quantity and quality for turfgrass use, research was conducted to determine the response of five warm-season turfgrasses to deficit irrigation and to gain a better understanding of relative drought tolerance. St. Augustinegrass(Stenotaphrum secundatum [Walt.] Kuntze.) cultivars 'Floratam' and 'Palmetto', 'SeaIsle 1' seashore Paspalum(Paspalum vaginatumSwartz.), 'Empire' zoysiagrass(Zoysia japonica Steud.), and 'Pensacola' bahiagrass(Paspalum notatum Flugge) were established in lysimeters in the University of Florida Envirotron greenhouse facility in Gainesville. Irrigation was applied at100%, 80%, 60%, or 40% of evapotranspiration(ET). Evaluations included: a) shoot quality, leaf rolling, leaf firing; b) leaf relative water content(RWC), soil moisture content, chlorophyll content index(CCI), canopy photosynthesis(PS); c) multispectral reflectance(MSR); d) root distribution; and e) water use efficiency. Grasses irrigated at 100% and 80% of ET had no differences in visual quality, leaf rolling, leaf firing, RWC, CCI, and PS. Grasses irrigated at 60% of ET had higher values in physiological aspects than grasses irrigated at 40% of ET. 'Sealsle 1' and 'Palmetto' had a deeper root system than 'Empire' and 'Pensacola', while 'Floratam' had the least amount of root mass. Photosynthesis was positively correlated with visual assessments such as turf quality, leaf rolling, leaf firing, and sensor-based measurements such as CCI, soil moisture, and MSR. Reducing the amount of applied water by 20% did not reduce turfgrass quality and maintained acceptable physiological functioning.

Measurement of fMCG Signals using an Axial Type First-Order SQUID Gradiometer System (권선형 1차 미분계를 이용한 태아심자도 신호 측정)

  • Yu, K.K.;Kim, K.;Kang, C.S.;Kim, J.M.;Lee, Y.H.
    • Progress in Superconductivity
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    • v.10 no.2
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    • pp.139-143
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    • 2009
  • We have fabricated a low-noise 61-channel axial-type first-order gradiometer system for measuring fetal magnetocardiography(MCG) signals. Superconducting quantum interference device(SQUID) sensor was based on double relaxation oscillation SQUID(DROS) for detecting biomagnetic signal, such as MCG, magnetoencphalogram(MEG) and fetal-MCG. The SQUID sensor detected axial component of fetal MCG signal. The pickup coil of SQUID sensor was wound with 120 ${\mu}m$ NbTi wire on bobbin(20 mm diameter) and was a first-order gradiometer to reject the environment noise. The sensors have low white noise of 3 $fT/Hz^{1/2}$ at 100 Hz on average. The fetal MCG was measured from $24{\sim}36$ weeks fetus in a magnetically shielded room(MSR) with shielding factor of 35 dB at 0.1 Hz and 80 dB at 100 Hz(comparatively mild shielding). The MCG signal contained maternal and fetal MCG. Fetal MCG could be distinguished relatively easily from maternal MCG by using independent component analysis(ICA) filter. In addition, we could observe T peak as well as QRS wave, respectively. It will be useful in detecting fetal cardiac diseases.

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A Study on Automatic Module Control Robot System (자율 모듈 제어 로봇시스템에 관한 연구)

  • Lee, Jeong-Ick
    • Proceedings of the KAIS Fall Conference
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    • 2010.05b
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    • pp.610-613
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    • 2010
  • 본 과제를 통하여 기술적으로는 로봇 개발에 있어 기존 기술을 최대한 활용하고 새로운 기능을 추가하는 형태로 발전해 갈 수 있도록, 각 요소별 기능을 표준화 모듈화 하여 새로운 제품의 개발이 손쉽게 이루어 질 수 있는 기본 platform으로의 역할이 가능해졌다. 구동부 제작 및 설계, 금형 비용 등을 절감하기 위한 다목적 완구로봇 플랫폼을 확보할 수 있었다. 로봇이 복잡한 환경을 인식하고, 자율적으로 작업을 수행하며, 예측하지 못한 문제에 적절한 대처를 할 수 있도록 하기 위한 핵심 기술을 제공하게 될 것으로 생각된다. 로봇 개발에 있어 전문지식이 없는 초보자들이 직접 제작 구동해 볼 수 있는 비주얼한 개발환경을 지원할 뿐만 아니라 GUI 개발환경 및 TEXT Programming 으로 상호 전환이 가능한 코딩 방법을 지원(MSRS와의 차별화를 시도하였다. 기존 하드웨어 지식과 소프트웨어지식이 없는 상태에서도 제작자의 창의력에 따라 다양한 형태의 로봇을 제작 적용이 용의 하며 초보자들에 대한 로봇개발에 흥미를 유발할 수 있도록 하였다. 고급사용자를 위한 별도의 다운로딩 포트를 제공하였다. 로봇공학의 기본지식을 습득(아날로그/디지털 회로를 이해하고, 각종 센서의 이해하며, 스템/서보/DC모터의 제어기술, 펌웨어개발능력, 기본수준의 소프트웨어개발 능력) 활용할 수 있다. 또한, 경제 및 산업적 측면으로는 다음과 같은 장점이 있다. 기존 기술들의 장점을 취합하여 추가적인 기술을 적용할 수 있는 Base형태의 로봇을 개발할 경우 이를 활용한 제품 개발 및 Base자체로도 충분한 시장을 형성 할 수 있을 것으로 기대된다. 인터넷기반 로봇 제어 기술의 확보로 로봇시스템의 생산비용을 낮추고 TTM (Time-to-Market) 제약을 해결시켜 준다. 다른 응용 시스템에 쉽게 적용이 가능하고 지식기반 로봇 서비스의 요소기술 개발은 멀티미디어 콘텐츠분야(교육/게임/오락 등)의 활성화가 가능하기 때문에 타 IT산업에의 파급효과가 예상된다. B2B를 통한 실질적인 판매를 통한 동종기업의 연구 개발비 및 개발 기간의 단축이 예상된다.

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A CEPHALOMETRIC STUDY ON FACIAL MORPHOLOGY IN ANGLE'S CLASS III MALOCCLUSION PATIENTS WITH FACIAL ASYMMETRY (안면비대칭을 동반한 Angle III급 부정교합자의 안모형태에 관한 두부방사선계측학적 연구)

  • Kim, Mee-Kyung;Kang, Jeung-Suk;Kim, Jong-Ryoul;Son, Woo-Sung
    • The korean journal of orthodontics
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    • v.24 no.4 s.47
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    • pp.787-798
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    • 1994
  • The purpose of this study was three-fold: i) to investigate the degree of asymmetry in Angle's Class III malocclusion patients and normal adults; ii) to determine the nature of difference existed between two groups; and iii) to investigate the correlationship between the degree of asymmetry and ANB and overbite in Angle's Class III malocclusion patients. The subjects consisted of 25 Angle's Class III malocclusion patients and 25 normal adults and the mean ages were 22.0 and 24.5 years, respectively. Their posteroanterior and lateral cephalograms were traced and analysed with three-dimensional approach. The results were as follows: 1. Asymmetry of Angle's Class III malocclusion group was significant in all regions except cranial base. Their horizontal asymmetry was seen in mandibular angle, maxillary and mandibular 1st molar, mandibular midline and menton. Vertical asymmetry was observed in maxillary 1st molar and mandibular shape and anteroposterior asymmetry in mandibular angle. 2. Nine variables indicating asymmetry were selected and each variable had similar discriminant score. 3. There was a little correlationship between An and asymmetric variable(MSR-B6) and its correlation coefficients was 0.3564. 4. There was no significant correlationship between overbite and asymmetric variables.

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Differential Symbiotic Response of Phage-typed Strains of Bradyrhizobium japonicum with Soybean Cultivars

  • Appunu Chinnaswamy;Dhar Banshi
    • Journal of Microbiology
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    • v.44 no.3
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    • pp.363-368
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    • 2006
  • In this study, native Byadyrhizobium strains were isolated from the host plant, Glycine max, harvested from fields in Madhya Pradesh, India, and were typed by Iytic rhizobiophages. Eight indigenous (Soy2, ASR011, ASR031, ASR032, MSR091, ISR050, ISR076 and ISR078) and two exotic strains (USDA123 and CB1809), all of which evidenced a distinct reaction with six phages, were employed in this study. The symbiotic interaction of these strains was studied initially using soybean cultivar JS335 in a sand culture in a controlled environment, and the efficiency was assessed based on the nodule number, nodule dry weight, plant dry weight, nitrogenase activity, and total accumulation of N per plant. Symbiotic effectiveness was found to be highest with the native phage-sensitive isolate ASR011, whereas it was at a minimum with the phage-resistant isolates, ISR050 and ISR078. Additionally, the effectiveness of these strains was evaluated using six soybean cultivars belonging to different maturity groups; namely, Brags, Lee, Pusa20, PK416, JS33S and NRC37. Analysis of variance data evidenced significant differences due to both symbionts, for the majority of the tested parameters. The CB1809, USDA123, and ASR011 strains evidenced relatively superior symbiotic effectiveness with soybean cultivars Brags, Lee and JS335. Strain ISR078 evidenced no significant responses with any of the cultivars. The ASR031 strain performed moderately well with all tested cultivars. The symbiotic response of all the strains was quite poor with cultivar PK416. Our studies showed that a significant relationship existed between the phage sensitivity and symbiotic efficiency of the bacterial strains with the host-cultivars.

Identification of Genes Modulated by High Extracellular Calcium in Coculture of Mouse Osteoblasts and Bone Marrow Cells by Oligo Chip Assay

  • Kim, Hyung-Keun;Song, Mi-Na;Jun, Ji-Hae;Woo, Kyung-Mi;Kim, Gwan-Shik;Baek, Jeong-Hwa
    • International Journal of Oral Biology
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    • v.31 no.2
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    • pp.53-65
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    • 2006
  • Calcium concentration in the bone resorption lacunae is high and is in the mM concentration range. Both osteoblast and osteoclast have calcium sensing receptor in the cell surface, suggesting the regulatory role of high extracellular calcium in bone metabolism. In vitro, high extracellular calcium stimulated osteoclastogenesis in coculture of mouse osteoblasts and bone marrow cells. Therefore we examined the genes that were commonly regulated by both high extracellular calcium and $1,25(OH)_2vitaminD_3(VD3)$ by using mouse oligo 11 K gene chip. In the presence of 10 mM $[Ca^{2+}]e$ or 10 nM VD3, mouse calvarial osteoblasts and bone marrow cells were co-cultured for 4 days when tartrate resistant acid phosphatase-positive multinucleated cells start to appear. Of 11,000 genes examined, the genes commonly regulated both by high extracellular calcium and by VD3 were as follows; 1) the expression of genes which were osteoclast differentiation markers or were associated with osteoclastogenesis were up-regulated both by high extracellular calcium and by VD3; trap, mmp9, car2, ctsk, ckb, atp6b2, tm7sf4, rab7, 2) several chemokine and chemokine receptor genes such as sdf1, scya2, scyb5, scya6, scya8, scya9, and ccr1 were up-regulated both by high extracellular calcium and by VD3, 3) the genes such as mmp1b, mmp3 and c3 which possibly stimulate bone resorption by osteoclast, were commonly up-regulated, 4) the gene such as c1q and msr2 which were related with macrophage function, were commonly down-regulated, 5) the genes which possibly stimulate osteoblast differentiation and/or mineralization of extracellular matrix, were commonly down-regulated; slc8a1, admr, plod2, lox, fosb, 6) the genes which possibly suppress osteoblast differentiation and/or mineralization of extracellular matrix, were commonly up-regulated; s100a4, npr3, mme, 7) the genes such as calponin 1 and tgfbi which possibly suppress osteoblast differentiation and/or mineralization of extracellular matrix, were up-regulated by high extracellular calcium but were down-regulated by VD3. These results suggest that in coculture condition, both high extracellular calcium and VD3 commonly induce osteoclastogenesis but suppress osteoblast differentiation/mineralization by regulating the expression of related genes.