• 대한한방내과학회지
• /
• 제21권2호
• /
• pp.243-250
• /
• 2000

Objectives : Yukmi-Jihwang-Tang(YJT) has been used in Oriental Medicine as a drug for tonifying and nourishing yin. So, the purpose of this Study was to investigate effects of Yukmi-Jihwang-Tang extract(YJTE) on the T-lymphocytes and peritoneal macrophages in mice. Methods : YJTE consists of the following components; Rehmaniae Radix Preparata(熟地黃), Dioscoreae Rhizoma(山藥), Corni-Fructus(山茱萸), Hoelen alba(白茯), Moutan Cortex Radicis(牧丹皮), Alismatis Rhizoma(澤瀉). Result : The results of this Study were obtained as follow; The administration of YJTE did not affect T-lymphocytes apoptosis. YJTE decreased sub-population of TH and TC cells, and proliferation of T-lymphocytes too. But YJTE accelerated phagocytic activity and Nitric Oxide(NO) production from peritoneal macrophages in mice. Conclusions : These results suggest that the administeration of YJTE suppresses T-cell mediated immunity, but activates peritoneal macrophages.

• 약학회지
• /
• 제8권2호
• /
• pp.54-58
• /
• 1964

Effects on the bacteria gwoth of 96 kinds of crude drugs described in "Pen-Tsao-Kang-Mu" was investigated. Galla Rhois, Moutan Cortex Radicis, Aconiti Tuber, Euphorbiae Radix and PAeoniae Radix showed the antibacterial activity but Cnidii Rhizoma, Coicis Semen, Angelicae gigantis Radix, Asparagi Radix and Liriopes Tuber show the gwoth promoting action of all bacteria used in this experience. It can be seen that 10 kinds of crude drugs classified as poisonous plants inhibited the growth or had no effect by never promoted. 32 kinds of crude drugs as drugs acting on the gastrointestinal system showed antibacterial activity on E. coli $O_{55}$ and E. coli $O_{111}$. 18 kinds on inflammation showed antibacterial activity on Staphylococcus aureus and Sarcina lutea.

• 대한약학회:학술대회논문집
• /
• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
• /
• pp.210.1-210.1
• /
• 2003

Keokhachukeu-tang is the one of Chinese traditional prescription used for the treatment of liver disease. This prescription consists of Carthami Flos (6g), Persicae Semen (9g), Pteropi Faeces (9g), Corydalis Tuber (9g), Moutan Radicis Cortex (6g), Paeoniae Radix rubra (9g), Angelicae gigantis Radix (9g), Cnidii Rhizoma (9g), Linderae Radix (12g), Cyperi Rhizoma (12g), Aurantii Fructus (9g), and Glycyrrhizae Radix (3g). Water extract of Keokhachukeu-tang showed a moderate hepatoprotective effect on tacrine-induced cytotoxicity in Hep G2 cells. (omitted)

• 동의생리병리학회지
• /
• 제20권5호
• /
• pp.1334-1336
• /
• 2006

Effects of twenty-three aqueous herbal extracts used in oriental medicines on heme oxygenase (HO)-1 expression were estimated in a mouse hippocampal cell line, HT22. HO-1 is one of the cytoprotective enzymes activated various stimuli, and Western blot analysis was used for evaluated HO-1 expression. Six aqueous extracts such as Rhei Rhizoma, Paeoniae Radix, Uncariae Ramulus et Uncus, Theae Folium, Prunellae Spica, and Coptidis Rhizoma significantly increased HO-1 expression in HT22 cells at the concentration of 300 ${\mu}$g/ml. In Addition, four aqueous extracts including Eucommiae Cortex, Moutan Cortex Radicis, Ginseng Radix Rubra, and Scutellariae Radix moderately increased HO-1 expression. These results would be usefulfor the isolation and identification of their neuroprotective principles.

• 한국식품위생안전성학회지
• /
• 제23권2호
• /
• pp.169-176
• /
• 2008

죽상경화증(arteriosclerosis)의 예방과 치료를 목적으로 조성된 새로운 한방처방인 WK-38을 웅성과 자성 랫트에 13주간 반복 투여하여 독성을 평가하였다. WK-38은 대황(大黃, Rhei Rhizoma), 후박(厚朴, Magonoliae Cortx), 목단피(牧丹皮, Moutan Cortex Radicis)의 복합물로 구성되었다. 실험동물에게 5 mg/kg, 50 mg/kg 또는 500 mg/kg을 경구로 투여하였다. 투여기간 동안 사망, 일반증상, 섭이량, 섭수량, 및 체중증가 등을 관찰하였다. 투여된 WK-38 모든 용량에서 사망하는 개체는 없었다. 시험기간 동안 체중의 지속적인 증가가 관찰되었으며 통계학적으로 유의적인 차이는 나타나지 않았다. 안검사 및 뇨검사에서 모든 투척군에서 대조군과 비교하여 시험물질 투여에 기인한 유의성 있는 변화는 관찰되지 않았다. WK-38 투여는 혈액학적 검사 및 혈액 생화학적 검사 결과 시험물질에 의한 독성학적 변화로는 판단되는 지표는 없었다. 이상의 결과에 근거하여 본 시험 조건하의 WK-38의 랫트에 대한 13주 반복 경구투여 시험에서는 독성학적 변화가 관찰되지 않았다. 따라서 무독성량은 500 mg/kg을 상회하는 것으로 판단된다.

• 한국자원식물학회지
• /
• 제9권3호
• /
• pp.261-267
• /
• 1996

모단(牡丹) 재배시(栽培時) 적합(適合)한 비료(肥料)를 선발(選拔)하여 재배기술(栽培技術) 체계(體系)를 확립(確立)하고자 묘두(苗頭) $2{\sim}3$ 개묘(個苗)의 제천지방재래종(堤川地方在來種)을 공시(供試)하였으며, 10a당(當) 퇴비(堆肥) 2,000kg, 3요소비료(要素肥料)($N-P_2O_5-K_2O=10-10-10$), 계분비료(鷄糞肥料) 100kg, 유기질비료(有機質肥料) 200kg, 2종복합비료(重複合肥料) 100kg, 무비구등(無肥區等) 6처리(處理)를 난괴법(亂塊法) 3반복(反復)으로 하여 1990년(年) 11월(月) 12일(日)에 휴폭(畦幅) 200cm(4열(列)), 주간(株間) 40cm(1주(株) 1본(本))으로 정식(定植)하여 시험(試驗)한 결과(結果)를 요약(要約)하면 다음과 같다. 1. 출현기(出現期)는 3월(月) 29일(日)로 처리간(處理間)에 차(差)없이 균일(均一)하였고, 입수율(立手率)은 85%이상(以上)으로 양호(良好)하였으며, 초장(草長)과 엽병장(葉柄長)은 무비구(無肥區) 58cm, 35cm보다 비료(肥料) 시용(施用)에서 길었고, 2년생(年生) 줄기의 길이는 13cm보다 2종복합비료(種複合肥料)에서 짧았으나 1년생(年生) 줄기 길이는 무비구(無肥區) 25cm보다 비료(肥料) 시용(施用)에서 모두 길었다. 2. 주근장(主根長)은 무비구(無肥區) 25.4cm에 비(比)하여 2종복합비료(種複合肥料) 시용(施用)에서 1.2cm가 짧았고, 그 외(外)의 비료시용(肥料施用)에서 $2.4{\sim}5.6cm$가 각각(各各) 길었으며, 근태(根太)는 무비구(無肥區) 1.3cm 보다 퇴비(堆肥) 유기질(有機質) 비료(肥料) 시용(施用)에서 가늘었으나, 화학비료(化學肥料) 시용(施用)에서는 굵었다. 3. 모단(牡丹)의 주성분(主成分)인 paeonol 함량(含量)은 무비구(無肥區)에 비(比)하여 3요소(要素)와 2종복합비료(種複合肥料), 유기질비료(有機質肥料) 시용(施用)에 의하여 오히려 낮아지는 경향(傾向)을 보였다. 4. 10a당 생근중(生根重)은 무비구(無肥區) 840kg에 비(比)하여 계분비료시용(鷄糞肥料施用)은 15%, 유기질(有機質) 비료(肥料)는 26%, 3요소 시용(施用)은 17%가 각각(各各) 증수(增收)되었으나, 특히 퇴비시용(堆肥施用)에서 31% 증수(增收)되었고, 근피중(根皮重)은 무비구(無肥區) 151kg에 비(比)하여 퇴비(堆肥), 계분(鷄糞) 비료(肥料) 시용(施用)은 $42{\sim}49%$ 증수(增收)되어 모단(牡丹) 재배시(栽培時) 화학비료(化學肥料) 시용(施用)보다는 퇴비(堆肥) 등(等) 유기질(有機質) 비료(肥料) 시용(施用)이 효고적(效果的)이었다.

• 동의생리병리학회지
• /
• 제17권4호
• /
• pp.905-913
• /
• 2003

Oriental medicine explains aging as the weakening of Kidney-ai, and Kidney-strengthening herbal medicines such as Yukmijihwang-tang have been studied for anti-aging effects. In Western Medicine, the hypothesis that reactive oxidant species(ROS) contribute to the aging process is generally accepted. It has been reported that Moutan Cortex Radicis extract (MCR) was the most effective constituent of Yukmijihwang-tang in decreasing ROS production in oxidative-stressed cells. The purpose of this study is to confirm the anti-oxidant effect of MCR on PC12 cells, the expression of Heme oxygenase (HO), Macrophage migradon inhibitory factor (MIF), Catechol-O-methyltransferase (COMT) using real time RT PCR. PC12 cells were treated without or with hydrogen peroxide in the presence or absence of MCR using MTS assay. Hydrogen peroxide decreased the viability of PC12 cells by 53% and MCR did not influence that of stressed PC 12 cells irrespective of dose or incubation period. However, MCR showed an inhibitory effect on production of ROS in stressed cells, both dose and incubation time dependently. In particular, 1 ㎎/㎖ of MCR for 24 h culture almost returned to normal level. In the quantiation of anti-aging related gene expression, MCR at 1 ㎎/㎖ increased the expression of HO by 370%, MIF by 180% and COMT by 280% through real time RT PCR. In conclusion, MCR treatment protected PC12 cells from hydrogen peroxide and decreased ROS production and enhanced anti-oxidative gene expression such as HO, COMT and MIF, which suggests that MCR is involved in controlling anti-aging of nerve cells through elimination of cytotoxic stimuli.

• Toxicological Research
• /
• 제21권1호
• /
• pp.63-70
• /
• 2005

Gami-Honghwa-Tang (KH-19), a herbal prescription for reducing the side effect of radiotherapy, is composed of eight crude herbs such as Rehmanniae Radix Preparata, Angelicae Gigantis Radix, Cnidii Rhizoma, Paeoniae Radix, Corni Fructus, Moutan Cortex Radicis, Lycii Fructus, Carthami Flos, and Glycyrrhizae Radix. In this study, marker substances in KH-19 were analyzed by high performance liquid chromatography-diode array detector (HPLC-DAD) and safety evaluation of standardized KH-19 was evaluated by good laboratory practices (GLP) guideline of Korea Food and Drug Administration. HPLC-DAD was employed to determine the quantities and the qualities of several marker substances such as 5-hydroxymethyl-2-furaldehyde (5-HMF), paeonol, loganin, paeoniflorin, glycyrrhizin, and decursin in the KH-19. In acute oral toxicity study on rat, transient inhibition of body weight was shown, but change in general symptom was not detected. No dead animal was observed up to 5,000 mg/kg in both male and female animals. In acute oral toxicity study on Beagle dog, transient vomiting, diarrhea, anorexia, and body weight reduction were observed, However, no dead animal was observed up to 2,000 mg/kg in both male and female animals.

• Biomolecules & Therapeutics
• /
• 제11권3호
• /
• pp.174-177
• /
• 2003

The present experiment was performed to investigate the protective effects of paeonol isolated from Moutan Cortex Radicis on primary cultured rat hepatocytes exposed to Br-A23187 ($Ca^{2+}$ ionophore). Br-A23187 is frequently used as a model of cell killing as inducing both necrotic and apoptotic cell death. Hepatocytes were isolated by collagenase perfusion from livers of fasted male Sprague Dawley rats and cultured overnight. Cell viability was determined by propidium iodide using fluorocytometry in Krebs-Ringer-HEPES buffer at pH 7.4. In addition, intracellular calcium was measured by excitation at 340 and 380 nm and emission at 505 nm using a luminescence spectrophotometer. Paeonol (20-100 ${\mu}M$) inhibited cell killing induced by 10 ${\mu}M$ Br-A23187, in a dose-dependent manner. Paeonol also reduced increased intracellular calcium level when hepatocytes were exposed to Br-A23187. Therefore, the present results suggest that paeonol protects the hepatocytotoxicity induced by Br-A23187, via inhibiting the influx of calcium into into rat hepatocytes.

• Biomolecules & Therapeutics
• /
• 제22권4호
• /
• pp.341-346
• /
• 2014

Paeonol is a major phenolic micromolecular component of Moutan cortex Radicis, a traditional Chinese Medicine. It has shown antitumor effects in previous studies; however, the underlying mechanisms remain unknown. This study investigated the mechanism by giving treatments of placebo, cyclophosphamide, paeonol of 150 and 300 mg/kg to 4 groups of mice bearing EMT6 breast cancer. Apoptosis in tumor cells were confirmed by morphology analysis, including hematoxylin, eosin staining and TUNEL staining. The results showed that the weight of EMT6 breast tumor was significantly reduced in the groups treated with both 150 and 300 mg/kg of paeonol. Immunohistochemical and Western blot results showed that the expression of Bcl-2 was down-regulated while the expression of Bax, caspase 8 and caspase 3 was up-regulated respectively. These results suggest that paeonol exhibits antitumor effects and the mechanism of the inhibition is via induction of apoptosis, regulation of Bcl-2 and Bax expression, and activation of caspase 8 and caspase 3.