Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Effect of Moutan Cortex Radicis on Gene Expression Profile of Differentiated PC12 Rat Cells Oxidative-stressed with Hydrogen Peroxide

모단피의 PC12 cell 항산화 효과와 관련 HO, MIF, COMT 유전자 발현에 미치는 영향

  • Son Mu Song (Department of Physiology, College of Oriental Medicine, Kyunghee University) ;
  • Rho Sam Woong (Department of Physiology, College of Oriental Medicine, Kyunghee University) ;
  • Ko Eun Jung (Department of Physiology, College of Oriental Medicine, Kyunghee University) ;
  • Na Youn Gin (Purimed R&D Institute) ;
  • Bae Hyun Su (Department of Physiology, College of Oriental Medicine, Kyunghee University, Purimed R&D Institute) ;
  • Hong Moo Chang (Department of Physiology, College of Oriental Medicine, Kyunghee University) ;
  • Shin Min Kyu (Department of Physiology, College of Oriental Medicine, Kyunghee University)
  • 손무성 (경희대학교 한의과대학 생리학교실) ;
  • 노삼웅 (경희대학교 한의과대학 생리학교실) ;
  • 고은정 (경희대학교 한의과대학 생리학교실) ;
  • 나영은 (퓨피메드(주) 기업부설연구소) ;
  • 배현수 (경희대학교 한의과대학 생리학교실, 퓨피메드(주) 기업부설연구소) ;
  • 홍무창 (경희대학교 한의과대학 생리학교실) ;
  • 신민규 (경희대학교 한의과대학 생리학교실)
  • Published : 2003.08.01
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Abstract

Oriental medicine explains aging as the weakening of Kidney-ai, and Kidney-strengthening herbal medicines such as Yukmijihwang-tang have been studied for anti-aging effects. In Western Medicine, the hypothesis that reactive oxidant species(ROS) contribute to the aging process is generally accepted. It has been reported that Moutan Cortex Radicis extract (MCR) was the most effective constituent of Yukmijihwang-tang in decreasing ROS production in oxidative-stressed cells. The purpose of this study is to confirm the anti-oxidant effect of MCR on PC12 cells, the expression of Heme oxygenase (HO), Macrophage migradon inhibitory factor (MIF), Catechol-O-methyltransferase (COMT) using real time RT PCR. PC12 cells were treated without or with hydrogen peroxide in the presence or absence of MCR using MTS assay. Hydrogen peroxide decreased the viability of PC12 cells by 53% and MCR did not influence that of stressed PC 12 cells irrespective of dose or incubation period. However, MCR showed an inhibitory effect on production of ROS in stressed cells, both dose and incubation time dependently. In particular, 1 ㎎/㎖ of MCR for 24 h culture almost returned to normal level. In the quantiation of anti-aging related gene expression, MCR at 1 ㎎/㎖ increased the expression of HO by 370%, MIF by 180% and COMT by 280% through real time RT PCR. In conclusion, MCR treatment protected PC12 cells from hydrogen peroxide and decreased ROS production and enhanced anti-oxidative gene expression such as HO, COMT and MIF, which suggests that MCR is involved in controlling anti-aging of nerve cells through elimination of cytotoxic stimuli.

Keywords

References

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