• 한국식품영양과학회지
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• 제31권1호
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• pp.155-159
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• 2002

AEY의 acetone 추출물이 mouse liver microsome의 산화에 미치는 영향을 조사하였다. Mouse liver microsome에 함유된 단백질 AEY 처리가 CEY 처리구에 비해 상대적으로 7~48%정도가 증가하였다. 지방산 분석 결과, CEY 처리에 비해 AEY 처리구에서 stearic acid를 포함하는 포화지방산의 함량비가 상대적으로 높았으며, oleic acid, linoleic acid 등을 함유하는 불포화지방산의 함량비가 현저히 감소하였다. AEY 처리구는 F $e^{2+}$가 관여하는 Asc/F $e^{2+}$, NADPH/F $e^{+2}$ 를 산화유도물질로 사용한 경우에 TC와 비슷하거나 보다 강력한 항산화 효과를 보였다. ABIN이나 CuOOH가 산화유도물질로 작용할 시에는 AEY처리에 의해 유의성있는 항산화 효과가 나타나지 않았다.

• 한국발생생물학회지:발생과생식
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• 제22권4호
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• pp.331-339
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• 2018

NUCB2/nesfatin-1 is first known to be expressed in the hypothalamus while controlling appetite and energy metabolism. However, recent studies have shown that NUCB2/nesfatin-1 was expressed in the various organs as well as the hypothalamus. Our previous reports also demonstrated that NUCB2/nesfatin-1 was expressed in the ovary, testis, pituitary gland, lung, kidney, and stomach of fetal and adult mice. However, the role of NUCB2/nesfatin-1 in mouse fetus remains unknown. Thus, the aim of this study was to investigate whether NUCB2/nestatin-1 is expressed in mouse fetus at the developmental stage in which organogenesis begins. To do this, we performed in situ hybridization (ISH) and immunohistochemistry (IHC) staining to examine the distribution of NUCB2 mRNA and nesfatin-1 protein in the mouse fetal organs during early developmental stages, especially at embryonic day (E) 10.5. As a result of ISH, NUCB2 mRNA positive signals were more frequent in the liver, but there were relatively few positive signals in heart. On the other hand, no positive signals were detected in other organs. These ISH results were validated by IHC staining and qRT-PCR analysis. Expression of nesfatin-1 protein detected by IHC staining was similar to that of NUCB2 mRNA detected by ISH in the liver and heart. In addition, the levels of NUCB2 mRNA expression analyzed by qRT-PCR were significantly increased in the liver and heart compared to other organs of the mouse fetus at E13.5, whereas its level was extensively decreased in the liver, but increased in the lung, stomach, and kidney of the mouse fetus at E17.5. These results suggest that NUCB2/nesfatin-1 may play an important role in liver and heart development and physiological functions in the developmental process of mouse fetus. Further studies are needed on the function of NUCB2/nesfatin-1, which is highly expressed in the various organs, including liver and heart during mouse development.

• Applied Microscopy
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• 제17권1호
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• pp.115-130
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• 1987

The present experiment was performed to investigate the acute effects of cadmium on ultrastructural and biochemical changes in mouse kidney and compare these changes with liver damage. Mouse were injected with cadmium chloride at a dose of 5 mg/kg body weight. After treatment, mouse were sacrificed at time intervals of 6, 12, 24 and 48 hours. It was observed that ultrastructural changes in mouse kidney were composed of swelling of mitochondria, dilation in endoplasmic reticulum, wrinkling at basal infolded membrane, formation of autophagosome and partial loss of microvilli in brush. border, and that ultrastructural changes in liver were mitochondrial change, dilation and deterioration of rough endoplasmic reticulum and proliferation of smooth endoplasmic reticulum. Biochemical effects of cadmium were more severe on liver than kidney. Therefore, acutely injected cadmium caused not only liver damage, but also kidney damage.

• 한국식품영양과학회지
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• 제12권2호
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• pp.84-92
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• 1983

자동 산화유가 mouse의 간장 효소에 미치는 영향을 구명하기 위하여 autoxidized methyl linoleate의 일정량을 mouse에게 1일 1회 20일 동안 경구 투여하여 간에서의 효소 활성을 조직화학적 및 생화학적 방법으로 검토한 결과는 다음과 같다. 실험군의 간에서의 POV, COV 및 TBA값은 정상군에 비해 크게 증가하여 AOML이 많이 흡수되어 있음을 알 수 있었다. 실험군의 간에서의 phospholipid, triglyceride 및 total cholesterol의 양은 정상군에 비해 증가하였다. 실험군의 간에서의 acid phosphatase, alkaline phosphatase의 활성은 정상군에 비해 증가하였으며, ATPase의 활성은 감소하였다. 실험군의 간에서의 형태학적 변화는 정상군에 비해 핵산의 감소, 지질의 축적 및 간 세포의 손상이 있었다. 이상의 결과로부터 mouse에 경구 투여한 자동 산화 methyl linoleate는 간에서의 효소 활성과 간의 형태학적 변화에 크게 영향을 주고 있음을 알 수 있었다.

• 한국자원식물학회지
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• 제8권3호
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• pp.275-280
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• 1995

Mouse의 $CCl_4$에 의한 급성간장해에 있어서 마늘분말은 농도의존적으로 간세포보호효과, 간독성억제효과를 갖는 것이 인정되었으며, 이러한 결과는 GOT, GPT등의 혈청학적 수치의 결과와 일치하였다. 그러나 마늘 extract 투여군에서의 간장 보호효과는 마늘분말보다도 미약하고 오히려 고농도 투여군인 10ml/kg 투여군에 비하여 저농도투여군인 1ml/kg에서 보다 뚜렷한 간장보호효과가 관찰되어 마늘 extract에 관한 약효, 약리작용에 관한 검토 및 독성에 관한 연구가 보다 필요한 것으로 사료되었다.

• 한국환경성돌연변이발암원학회지
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• 제26권3호
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• pp.86-88
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• 2006

Cytochrome P450 1A2 (CYP1A2) is a xenobiotic metabolizing enzyme that is tissue-specifically expressed in the mammalian liver. In this study, the extent of CYP1A2 promoter methylation was analyzed to determine its potential role in the regulation of CYP1A2 in diethylnitrosamine (DEN)-induced mouse liver tumors. CYP1A2 mRNA was under-expressed about three fold in DEN-induced liver tumors compared to age-matched control livers. The CYP1A2 promoter was hypermethylated in DEN-induced liver tumors compared to controls, especially in a promoter domain close to the coding region. These results suggest that promoter methylation is involved in the regulation of CYP1A2 in mouse liver tumors.

• 한국식품영양학회지
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• 제17권4호
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• pp.442-449
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• 2004

The purpose of this study was to examine the antioxidative activities and tissue cell of mouse liver added caffeine. The body weight of all experimental groups increased during experimental periods, but the body weight of caffeine-containing groups were lower than those of any other experimental groups. Superoxide dismutase and catalase activities tended to decrease significantly with caffeine-containing groups, but increased in control and ginseng-containing groups. Hydroperoxide contents were increase significantly with caffeine-containing groups. Lipid peroxidation levels decreased in ginseng-containing groups, but it increased significantly with caffeine-containing group. Protein contents were a tendency of similar between control and ginseng-containing groups, but it showed a increasing tendency in caffeine-containing groups. Microscopic observation of mouse liver cell were similar tissue in ginseng and caffeine-containing groups, but it showed somewhat more injuring only at the liver cell of anhydrous caffeine group, and became the suspicion in liver diseases. This results show that antioxidative activities are slightly higher in non-caffeine and ginseng-containing drinks than caffeine-cotaining drinks. From this standpoint, we suggest that too much drinking of caffeine-containing drinks for a long time is undesirable.

• 한국식품영양과학회지
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• 제13권2호
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• pp.169-174
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• 1984

해바라기 화분립이 mouse의 간장에서 cholesterol 대사에 어떤 영향을 미치는가를 조사하기 위해서 해바라기 화분립으로부터 추출한 지질을 column chromatography, thin-layer chromatography 및 gas-liquid chromatography를 이용해서 분석했다. 또한 mouse에 화분립을 투여하였을 때 간장에서의 cholesterol치에 미치는 영향에 대해서도 조사하였다. 1. 화분립의 지질은 neutral lipid 84.10%, glycolipid 10.50%, phospholipid 5.40%를 함유했다. 2. 중성지방질의 조성은 triglyceride가 71.20%로 가장 많고, sterol ester, diglyceride, monoglyceride, free fatty acid, sterol의 순이다. 3. neutral lipid, glycolipid, phospholip의 주요지방산은 linoleic acid 28.48${\sim}$33.70%, palmitic acid 12.90${\sim}$47.50%, oleic acid 11.20${\sim}$12.20% 이다. 그러나 phospholipid는 다른 지질보다 palmitic acid를 더 많이 함유하고 있다. 4. 해바라기 화분립 투여 mouse의 체중은 대조군 보다 현저하게 증가했다. 5. 화분립을 투여한 mouse의 간장지질대사가 대조군보다 활발함을 알 수 있었다. 6. 화분립 투여 mouse의 간 cholesterol치가 초기에는 증가했으나, 말기에는 대조군과 비슷한 수준으로 감소했다.

• The Korean Journal of Physiology
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• 제6권2호
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• pp.57-63
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• 1972

In an attempt to better understand the radioprotective effect of reduced glutathione(GSH), and to observe a possible radioprotective effect of Ginseng extract, whole body X-irradiation of 1,200 r was administered to the mouse either independently or immediately following the injection of GSH or Ginseng extract to the mouse intraperitoneally. The non-protein sulfhydryl (NP-SH) and non-protein disulfide (NP-SS) levels of the liver, and NP-SH level of NP-SH of the blood of the mouse were measured at 30, 60 and 120 minutes, and results were compared with the normal. The results thus obtained are summarized as follows; 1) The normal values of NP-SH and NP-SS of the mouse liver were $5.90{\pm}0.46\;{\mu}\;mol/gm\;wet\;wt.,\;and\;3.02{\pm}0.42\;{\mu}\;mol/ml$ wet wt., respectively, and the normal value of NP-SH of NP-SH of the mouse blood was $3.98{\pm}1.29\;{\mu}\;mol/ml$ 2) The injection of both GSH and Ginseng extract produced the highest values of NP-SH in the liver at 30 minutes, but a gradual decrease to the normal was observed thereafter. When X-irradiation alone was applied, the liver NP-SH value was lower than the normal at 60 minutes post-irradiation and thereafter. When Ginseng extract was injected immediately prior to X-irradiation, the liver NP-SH was lower than the normal throughout the experiment with the lowest value at 60 minutes. However, the combination of GSH and X-irradiation produced higher than the normal values throughout the entire experiment. 3) The liver NP-SS value was most significantly elevated at 30 minutes after the injection of GSH, hut the recovery to the normal was observed thereafter. The injection of Ginseng extract produced slightly higher liver NP-SS values at 30 and 60 minutes, but the value at 120 minutes was similar to the normal. The single application of X-irradiation resulted in the lower then normal liver NP-SS values throughout the entire experiment. When GSH was injected price to X-irradiation, the liver NP-SS values were higher than the normal at 30 and 60 minutes followed b the recovery to the normal at 120 minutes. The combination of Ginseng extract and X-irradiation showed generally lower liver NP-SS values throughout the experiment. 4) The blood NP-SH showed the higher than the normal values in all the experimental groups except when GSH was injected prior to X-irradiation alone produced e significantly elevated blood NP-SS value at 30 minutes post-irradiation.

• 동의생리병리학회지
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• 제22권2호
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• pp.282-289
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• 2008

We evaluated the effect of SHBCS on adhesion and invasion of colon L5-26 adenocarcinoma cell line in vitro in vitro and experimental liver metastasis in vivo. SHBCS showed little inhibitory effect on colon 26-L5 cell proliferation. At the concentration of up to 500 mg/ml of SHBCS 80% of cells were viable. SHBCS showed no inhibitory effect on adhesion and invasion of colon 26-L5 cells, which were placed on matrigel. In a dose dependent manner, oral administration of SHBCS showed a significantly inhibitory effect on liver metastasis from colon 26-L5 injected mice. When mice were depleted of NK cells or macrophages before tumor inoculation, SHBCS significantly decreased liver metastasis fromf the tumor injected mice. Compared with the control mice, SHBCS increased the populations of macrophages and NK cells by 30%, 18%(10 mg/mouse, 50 mg/mouse) and 5%, 1% (10 mg/mouse, 50 mg/mouse) respectively. Compared with the control mice, SHBCS increased the populations of CD4 cells by 5%, 18% (10 mg/mouse, 50 mg/mouse) respectively. Spelenocytes from mice administerd with SHBCS were stimulated with LPS plus ConA, proliferation of splenocytes from mice administerd with SHBCS was 140%, 146%(10 mg/mouse, 50 mg/mouse) compared with th control mice. In conclusion, the present study suggests that SHBCS may have an inhibitory effect on liver metastasis through immunopotentiating activity which is associated with macrophages and NK cells.