Effect of Egg Yolks from Laying Hens Intubated Astaxanthin on the Oxidation of Liver Microsome of Mouse

Effect of the egg yolky from laying hens intubated, p.o., astaxanthin (designated AEY) on the oxidation of mouse liver microtome was investigated using female ICR mouse (6~7 weeks of age). Mice were adapted in a temperature- and humidity-controlled house for one week and randomly divided into 7 treatment groups (10 mice/cage/treatment). Mice were intubated p.o., AEY (50, 100 and 150 mg) or control egg yolk (CEY, 150 mg) every week for 3 weeks. BHT (5 mg) and e -tocopherol (50 mg) were fed to mice as positive control. At week 4, the liver microsome was prepared from sacrificed mice. Protein content of mouse liver microsome with AEY treatment was relatively higher than that with CEY treatment. AEY treatment remarkably lowered the content of unsaturated fatty acids including oleic acid and linoleic acid, but raised that of the saturated fatty acids including stearic acid. AEY group showed relatively higher antioxidative activity than CEY, when used Asc/F $e^{+2}$ or NADPH/F $e^{+2}$ as oxidant. Antioxidative activity of AEY was more effective than $\alpha$-tocopherol, but less effective than BHT.

Astaxanthin처리 산란계로부터 생산된 난황이 Mouse Liver Microsome의 산화에 미치는 영향

AEY의 acetone 추출물이 mouse liver microsome의 산화에 미치는 영향을 조사하였다. Mouse liver microsome에 함유된 단백질 AEY 처리가 CEY 처리구에 비해 상대적으로 7~48%정도가 증가하였다. 지방산 분석 결과, CEY 처리에 비해 AEY 처리구에서 stearic acid를 포함하는 포화지방산의 함량비가 상대적으로 높았으며, oleic acid, linoleic acid 등을 함유하는 불포화지방산의 함량비가 현저히 감소하였다. AEY 처리구는 F $e^{2+}$가 관여하는 Asc/F $e^{2+}$, NADPH/F $e^{+2}$ 를 산화유도물질로 사용한 경우에 TC와 비슷하거나 보다 강력한 항산화 효과를 보였다. ABIN이나 CuOOH가 산화유도물질로 작용할 시에는 AEY처리에 의해 유의성있는 항산화 효과가 나타나지 않았다.