Effect of Sanghongbaekchul-san on Anti-metastatic and Immunopotentiating Activities

상홍백출산(桑紅白朮散)이 생쥐 대장암세포의 간전이억제와 면역활성화에 미치는 효과

  • Oh, Se-Soon (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University, Institute of Oriental Medicine, Kyunghee University) ;
  • Kang, Hee (Brain Korea 21 Oriental Medical Science Center) ;
  • Shim, Bum-Sang (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University, Institute of Oriental Medicine, Kyunghee University) ;
  • Kim, Sung-Hoon (Cancer Preventive Material Development Research Center) ;
  • Choi, Seung-Hoon (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University, Institute of Oriental Medicine, Kyunghee University) ;
  • Ahn, Kyoo-Seok (Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University, Institute of Oriental Medicine, Kyunghee University)
  • 오세순 (경희대학교 한의과대학 병리학교실, 경희대학교 한의학연구소) ;
  • 강희 (BK21경희대학교 한의과학사업단) ;
  • 심범상 (경희대학교 한의과대학 병리학교실, 경희대학교 한의학연구소) ;
  • 김성훈 (암예방소재개발연구센터) ;
  • 최승훈 (경희대학교 한의과대학 병리학교실, 경희대학교 한의학연구소) ;
  • 안규석 (경희대학교 한의과대학 병리학교실, 경희대학교 한의학연구소)
  • Published : 2008.04.25

Abstract

We evaluated the effect of SHBCS on adhesion and invasion of colon L5-26 adenocarcinoma cell line in vitro in vitro and experimental liver metastasis in vivo. SHBCS showed little inhibitory effect on colon 26-L5 cell proliferation. At the concentration of up to 500 mg/ml of SHBCS 80% of cells were viable. SHBCS showed no inhibitory effect on adhesion and invasion of colon 26-L5 cells, which were placed on matrigel. In a dose dependent manner, oral administration of SHBCS showed a significantly inhibitory effect on liver metastasis from colon 26-L5 injected mice. When mice were depleted of NK cells or macrophages before tumor inoculation, SHBCS significantly decreased liver metastasis fromf the tumor injected mice. Compared with the control mice, SHBCS increased the populations of macrophages and NK cells by 30%, 18%(10 mg/mouse, 50 mg/mouse) and 5%, 1% (10 mg/mouse, 50 mg/mouse) respectively. Compared with the control mice, SHBCS increased the populations of CD4 cells by 5%, 18% (10 mg/mouse, 50 mg/mouse) respectively. Spelenocytes from mice administerd with SHBCS were stimulated with LPS plus ConA, proliferation of splenocytes from mice administerd with SHBCS was 140%, 146%(10 mg/mouse, 50 mg/mouse) compared with th control mice. In conclusion, the present study suggests that SHBCS may have an inhibitory effect on liver metastasis through immunopotentiating activity which is associated with macrophages and NK cells.

Keywords

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