• IMMUNE NETWORK
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• v.3 no.2
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• pp.126-135
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• 2003

Background: One of the important factors in the prognosis of chronic hepatitis B patient is the degree of replication of hepatitis B virus (HBV). It has been known that HBV DNA polymerase plays the essential role in the replication of HBV. HBV DNA polymerase is composed of four domains, TP (Terminal protein), spacer, RT (Reverse transcriptase) and RNaseH. Among these domains, tyrosine, the $65^{th}$ residue of TP is an important residue in protein-priming reaction that initiates reverse transcription. If monoclonal antibody that recognizes around tyrosine residue were selected, it could be applied to further study of HBV replication. Methods: To produce TP-specific scFv (single-chain Fv) by phage display, mice were immunized using synthetic TP-peptide contains $57{\sim}80^{th}$ amino acid residues of TP domain. After isolation of mRNA of heavy-variable region ($V_H$) and light-chain variable region ($V_L$) from the spleen of the immunized mouse, DNA of $V_H$ and $V_L$ were obtained by RT-PCR and joined by a DNA linker encoding peptide (Gly4Ser)3 as a scFv DNA fragments. ScFv DNA fragments were cloned into a phagemid vector. scFv was expressed in E.coli TG1 as a fusion protein with E tag and phage gIII. To select the scFv that has specific affinity to TP-peptide from the phage-antibody library, we used two cycles of panning and colony lift assay. Results: The TP-peptide-specific scFv was isolated by selection process using TP-peptide as an antigen. Selected scFv had 30 kDa of protein size and its nucleotide sequences were analyzed. Indirect- and competitive-ELISA revealed that the selected scFv specifically recognized both TP-peptide and the HBV DNA polymerase. Conclusion: The scFv that recognizes the TP domain of the HBV DNA polymerase was isolated by phage display.

• Reproductive and Developmental Biology
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• v.36 no.3
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• pp.155-161
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• 2012

Germ cell-specific hyaluronidases such as sperm adhesion molecule 1 (SPAM1) and hyaluronoglucosaminidase 5 (Hyal5) are in part responsible for dispersal of the cumulus cell mass, which is a critical step in establishing fertilization in mammals. In this study, we identified two testis-hyaluronidases, SPAM1 and Hyal5, in hamster and rat. These two genes were expressed specifically in the testis. At the protein level, hamster SPAM1 and Hyal5 display 78.7% and 75.4% identity with mouse SPAM1 and Hyal5. Further, the activity of the enzymes with respect to cumulus cell dispersion did not differ, although we observed that the enzymatic activity differed in pH range. These studies suggest that different sperm hyaluronidases are capable of dispersing the cumulus cell mass despite differences in enzyme activity.

• Proceedings of the IEEK Conference
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• 2003.11a
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• pp.241-244
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• 2003

Nowadays image processing is very useful for some field of traffic applications. The one reason is we can construct the system in a low price, the other is the improvement of hardware processing power, it can be more fast to processing the data. In this study, I propose the traffic monitoring system that implement on the embedded system environment. The whole system consists of two main part, one is host controller board, the other is image processing board. The part of host controller board take charge of control the total system, interface of external environment. and OSD(On screen display). The part of image processing board takes charge of image input and output using video encoder and decoder, image classification and memory control of using FPGA, control of mouse signal. And finally, fer stable operation of host controller board, uC/OS-II operating system is ported on the board.

• Proceedings of the IEEK Conference
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• 2003.11b
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• pp.83-86
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• 2003

This paper suggests user's interface with computer by means of detecting gaze under HMD, head mounted display, environment. System is derived as follows; firstly, calibrate a camera in HMD, which determines geometrical relationship between monitor and captured image. Second, detect the center of pupil using algorithm of the center of mass and represent a gazing position on the computer screen. If user blinks or stares at a certain position for a while, message is sent to computer. Experimental results show the center of mass is robust against glint effects, and detecting error was 7.1%. and 4.85% in vertical and horizontal direction, respectively. To adjust detailed movement of a mouse takes 0.8 sec more. The 98% of blinking is detected successfully and 94% of clicking detection is resulted.

• Proceedings of the KOSOMBE Conference
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• v.1998 no.11
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• pp.193-194
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• 1998

This paper describes a multi-functional EEG (electroencephalogram) software. The software manages the patient's EEG data systematically and analyzes the signal and display the parameters on a PC monitor in real-time. Since the software provides various parameters simultaneously, user can observe patients multilaterally. Reference patterns of CSA and DSA can be captured and displayed on top of the monitor. And user can mark events of surgical operation or patient's conditions, so it is possible to jump to the points of events directly, when reviewing the recorded file afterwards. Many convenient functions are equipped and these are operated by mouse clicks.

• Proceedings of the Korean Society of Computer Information Conference
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• 2015.07a
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• pp.196-197
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• 2015

본 논문에서는 산업용 컴퓨터에서 사용할 마우스와 터치스크린을 이용한 산업용 컴퓨터 인터페이스 장치 구현에 대하여 연구하였다. 산업용 제어 현장에서 사용되는 제어용 컴퓨터의 입출력 장치는 확장성을 고려하여 설계되지 않았기 때문에 현재의 컴퓨터 주변장치를 직접 사용할 수 없는 경우가 대부분이다. 본 논문에서는 산업용컴퓨터에서 사용되는 라이트펜과 모니터를 현재 주로 사용하는 마우스와 LCD모니터로 입출력이 가능하도록 하는 인터페이스 장치를 개발하여, 생산 공정에서 사용하는 산업용 컴퓨터의 활용도를 높이고자 한다. 산업용 컴퓨터 인터페이스장치는 ARM 기반 MPU를 이용하여 영상 신호와 외부 입력장치의 자료를 처리하고, CPLD에서 생성된 신호를 제어용 컴퓨터로 입력하였다. 제어컴퓨터의 영상신호를 다양한 모니터와 인터페이스 할 수 있도록 비디오 스케일러(Video Scaler)를 사용하였다. 구현한 인터페이스 장치는 다양한 장치들을 산업용컴퓨터에서 사용이 가능함을 보였다.

• Bulletin of the Korean Chemical Society
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• v.20 no.9
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• pp.1073-1077
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• 1999

CRAMP, a 37-amino acid cationic antimicrobial peptide was recently deduced from the cDNA cloned from mouse femoral marrow RNA. In order to investigate the structure-activity relationship and functional region of CRAMP, CRAMP and its 18-mer overlapping peptides were synthesized by the solid phase method. CRAMP showed broad spectrum antibacterial activity against both Gram-positive and Gram-negative bacterial strains (MIC: 3.125-6.25 μM) but had no hemolytic activity until 50 μM. CRAMP was found to have a potent anticancer activity (IC50: 12-23 μM) against two human small cell lung cancer cell lines. Furthermore, CRAMP was found to display faster bactericidal rate in B. subtilis rather than E. coli in the kinetics of bacterial killing. Among 18-meric overlapping fragment peptides, only CRAMP (16-33) displayed potent antibacterial activity (MIC: 12.5-50 μM) against several bacteria with no hemolytic activity. Circular dichroism (CD) spectra anal-ysis indicated that CRAMP and its analogues will form the amphipathic α-helical conformation in the cell membranes similar to other antimicrobial peptides, such as cecropins and magainins.

• Animal cells and systems
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• v.3 no.3
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• pp.319-329
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• 1999

Onset of female puberty follows a series of prepubertal cellular and molecular events including changes of synaptic plasticity, synthetic and releasing activity and gene expression. Dramatic increase of gonadal steroid level is one of the most prominent changes before the onset of puberty. Based on the importance of steroid feedback upon the hypothalamus, we adopted an estrogen sterilized rat (ESR) model where 100 ng of 17$\eta$-estradiol were administered into neonatal pubs for 7 days after birth. To identify genes involved in the onset of female puberty, we applied PCR differential display using RNA samples derived from ESR and control rat hypothalami. About 100 out of more than 1000 RNA species examined displayed differential expression patterns between a 60-day old control rat and ESR. Sequence analysis of differentially amplified PCR products showed homology with genes such as mouse kinesin superfamily-associated protein 3 (KAP3) and several cDNAs previously described by others in mouse and human tissues. Several gene products such as 2-1 and 8-1 corresponded to novel DNA sequences. We analyzed mRNA levels of KAP3, 2-1 and 8-1 genes in the hypothalami derived from neonatal, 6-, 28-, 31-, and 40-day old rats. Northern blot analysis showed that mRNAs of KAP3, 2-1 and 8-1 genes were markedly increased before the initiation of puberty. Neonatal treatment of estrogen clearly inhibited prepubertal increases in KAP3, 2-1 and 8-1 mRNA levels. Therefore, these genes may play important roles in the initiation of hypothalamic puberty. In addition, intracerebroventricular (icv) injection of antisense KAP3 oligodeoxynucleotide (ODN) clearly delayed puberty initiation determined by vaginal opening, which further confirmed that KAP3 plays an important role in the regulation of puberty initiation.

• Korean Journal of Biological Psychiatry
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• v.6 no.1
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• pp.67-73
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• 1999

The cAMP-dependent protein kinase(PKA) is an intracellular enzyme with serine-threonine kinase activity that plays a key role in cell growth, differentiation, and apoptosis in eukaryotes. In order to understand the PKA signal transduction pathway regulating cell life cycle and identify its role, we focused on the characterization of up-/down-regulated genes by PKA using the differential display polymerase chain reaction. Seven differentially expressed sequence tags(DEST) have been obtained. Among these DESTs, 2 DESTs were homologous to the sequence of genes from BLAST search result. KC1-5 DEST that was up-regulated in A123.7 cells was highly corresponded to mouse apoptosis-related gene(MA-3) or mouse mRNA for topoisomerase inhibitor suppressed(TIS). MA-3 was induced in various types of apoptosis, specially in NGF-deprived apoptotic PC12 cells. TIS was down-regulated in the RVC lymphoma cells incubated with topoisomerase inhibitor that induces DNA strand breakages. PG1-1 DEST that was highly expressed in PC12 cells was corresponded to transposon Tn10 3'-end. Tnansposon Tn10 was up-regulated in differentiated myeloblastic ML-1 cells by 12-O-tetradecanoylphorbol-13-acetate. This study illuminates that MA-3/TIS was down-regulated by PKA activity, and transposon Tn10 was up-regulated by it.

• Journal of Korean Ophthalmic Optics Society
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• v.15 no.3
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• pp.257-262
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• 2010

Purpose: Developing a calculating program for the prism power which influenced the binocular vision according to shifts of binocular visual points in the distance vision spectacles. Methods: By using the Delphi 6.0 programming language, we developed a calculating program of the relative binocular prism power according to the movements of binocular visual points in the distance vision spectacles, which was calculated by dragging the mouse along the traces of binocular visual points on the computer window. Results: We developed a calculating program for the relative binocular prism power according to the movements of binocular visual points in the distance vision spectacles. The user of the program could confirm the trace of visual points by allowing them to display the trace of binocular visual points on the computer screen with a mouse button. An application on confirming the variation of prism power by graphs in the program also allowed the user to use the program more conveniently. Conclusions: By using the developed program, the user could easily calculate the relative binocular prism power according to shifts of binocular visual points in the distance vision spectacles. We also found that the developed program helped the user to receive a lot of assistance in analyzing the asthenopia.