Korean Journal of Biological Psychiatry (생물정신의학)

The Korean Society of Biological Psychiatry (대한생물정신의학회)
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Screening of Differentially Expressed Genes between PC12 Cells and A123.7 Cells

PC12 세포와 A123.7 세포에서 차별적으로 발현되는 유전자의 검색

  • Baik, Seung-Youn (Department of Biochemistry and Molecular Biology, College of Science, Hanyang University) ;
  • Yang, Byung-Hwan (Department of Neuropsychiatry, College of Medicine, Hanyang University) ;
  • Chai, Young-Gyu (Department of Biochemistry and Molecular Biology, College of Science, Hanyang University)
  • 백승연 (한양대학교 이과대학 생화학 및 분자생물학과) ;
  • 양병환 (한양대학교 의과대학 신경정신학과교실) ;
  • 채영규 (한양대학교 이과대학 생화학 및 분자생물학과)
  • Published : 1999.06.25
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Abstract

The cAMP-dependent protein kinase(PKA) is an intracellular enzyme with serine-threonine kinase activity that plays a key role in cell growth, differentiation, and apoptosis in eukaryotes. In order to understand the PKA signal transduction pathway regulating cell life cycle and identify its role, we focused on the characterization of up-/down-regulated genes by PKA using the differential display polymerase chain reaction. Seven differentially expressed sequence tags(DEST) have been obtained. Among these DESTs, 2 DESTs were homologous to the sequence of genes from BLAST search result. KC1-5 DEST that was up-regulated in A123.7 cells was highly corresponded to mouse apoptosis-related gene(MA-3) or mouse mRNA for topoisomerase inhibitor suppressed(TIS). MA-3 was induced in various types of apoptosis, specially in NGF-deprived apoptotic PC12 cells. TIS was down-regulated in the RVC lymphoma cells incubated with topoisomerase inhibitor that induces DNA strand breakages. PG1-1 DEST that was highly expressed in PC12 cells was corresponded to transposon Tn10 3'-end. Tnansposon Tn10 was up-regulated in differentiated myeloblastic ML-1 cells by 12-O-tetradecanoylphorbol-13-acetate. This study illuminates that MA-3/TIS was down-regulated by PKA activity, and transposon Tn10 was up-regulated by it.

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