• Journal of Life Science
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• v.33 no.11
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• pp.868-875
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• 2023

Kinesin-1 is a motor protein identified as the first member of the kinesin superfamily (KIF), which plays a role in intracellular cargo transport by acting as microtubule-dependent motor proteins within cells. Kinesin-1 consists of two heavy chains (KHCs, also known as KIF5s) and two light chains (KLCs). The 93 amino acids in the carboxyl (C)-terminal tail region of KIF5A are not homologous to the C-terminal tail region of KIF5B or the C-terminal tail region of KIF5C. In this study, we used a yeast two-hybrid screen to identify the binding proteins that interacted with the C-terminal region of KIF5A. We found an association between KIF5A and CUE domain containing 2 (CUEDC2), which is proposed to function as an adaptor protein involved in ubiquitination pathways and protein trafficking. CUEDC2 bound to the C-terminal region of KIF5A and did not interact with KIF5B (the motor of kinesin-1), KIF3A (the motor of kinesin-2), or kinesin light chain 1 (KLC1). KIF5A specifically bound to the C-terminal region of CUEDC2. Furthermore, KIF5A did not interact with another isoform: CUEDC1. In addition, glutathione S-transferase (GST) pull-downs showed that KIF5A directly bound GST-CUEDC2 but did not interact with GST-CUEDC1 and GST alone. When myc-KIF5A and EGFP-CUEDC2 were co-expressed in HEK-293T cells, CUEDC2 co-immunoprecipitated with kinesin-1, and myc-KIF5A and FLAG-CUEDC2 colocalized in the cells. These results suggest that in intracellular cargo transport by kinesin-1, CUEDC2 serves as an adaptor protein connecting kinesin-1 and cargo by binding to KIF5A.

• Journal of rehabilitation welfare engineering & assistive technology
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• v.2 no.1
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• pp.35-43
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• 2009

In this paper, we introduce a reading training program offering visual-auditory cue with noise cancellation function (RT program) developed by us. The RT program provides some training sentences with visual-auditory cues. Motor speech disorder patients can use the visual and/or auditory cues for reading training. To provide convenient estimation of training result, we developed a noise cancellation algorithm. The function of the algorithm is to remove noise and auditory-cues which are recorded with reading speech at the same time while patient read the sentences in PC monitor. In addition, we developed a function for finding out the first starting time of reading sound after a patient sees a sentence and begins to read the sentence. The recorded speeches are acquired from six people(three male, three female) in four noisy environments (interior noise, white noise, car interior noise, babble noise). We evaluated the timing error for starting time between original recorded speech and processed speech in condition of executing noise cancellation function and not executing. The timing error was improved as much as $4.847{\pm}2.4235[ms]$ as the effect of noise cancellation. It is expected that the developed RT program helps motor speech disorder patient in reading training and symptom evaluation.

• Journal of Biomedical Engineering Research
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• v.30 no.5
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• pp.428-437
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• 2009

The Performance research about EEG-BCI algorithm in BCI-naive subjects is very important for evaluating the applicability to the public. We analyzed the result of the performance evaluation experiment about the EEG-BCI algorithm in BCI-naive subjects on three different aspects. The EEG-BCI algorithm used in this paper is composed of the common spatial pattern(CSP) and the least square linear classifier. CSP is used for obtaining the characteristic of event related desynchronization, and the least square linear classifier classifies the motor imagery EEG data of the left hand or right hand. The performance evaluation experiments about EEG-BCI algorithm is conducted for 40 men and women whose age are 23.87${\pm}$2.47. The performance evaluation about EEG-BCI algorithm in BCI-naive subjects is analyzed in terms of the accuracy, the relation between the information transfer rate and the accuracy, and the performance changes when the different types of cue were used in the training session and testing session. On the result of experiment, BCI-naive group has about 20% subjects whose accuracy exceed 0.7. And this results of the accuracy were not effected significantly by the types of cue. The Information transfer rate is in the inverse proportion to the accuracy. And the accuracy shows the severe deterioration when the motor imagery is less then 2 seconds.

• Korean Journal of Cognitive Science
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• v.27 no.4
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• pp.573-597
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• 2016

Recent fMRI and EEG research for neural representations of action concepts insist that processing of action concepts evoke the simulation of sensory-motor information. Moreover, there are several behavioral studies showing that understanding of action verbs or sentences describing actions interfere or facilitate current action performance. However, it is unclear that online interaction between processing of action concepts and current action is based on the simulation of sensory-motor information, or other neural mechanisms. The present research aims to explore the underlying neural mechanism that how the perception of action language influence the performance of current action using high-spacial temporal resolution EEG and multiple source analysis techniques. For this, participants were asked to perform a cued-motor reaction task in which button-pressing hand action and pedal-stepping foot action were required according to the color of the cue, and we presented auditorily action verbs describing the responding actions (i.e., /press/, /step/, /stop/) just before the color cue and examined the interaction effect from the semantic congruency between the action verbs and the current action. Behavioral results revealed consistently a facilitatory effect when action verbs and responding actions were semantically congruent in both button-pressing and pedal-stepping actions, and an inhibitory effect when semantically incongruent in the button-pressing action condition. In the results of EEG source waveform analysis, the semantic congruency effects between action verbs and the responding actions were observed in the Wernicke's area during the perception of action verbs, in the anterior cingulate gyrus and the supplementary motor area (SMA) at the time when the motor-cue was presented, and in the SMA and primary motor cortex (M1) during action execution stage. Based on the current findings, we argue that perceived action verbs evoke the facilitation/inhibition effect by influencing the expectation and preparation stage of following actions rather than the directly activating the particular motor cortex. Finally we discussed the implication on the neural representation of action concepts and methodological limitations of the current research.

• Journal of Music and Human Behavior
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• v.11 no.1
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• pp.1-19
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• 2014

The purpose of this study was to investigate the immediate effect of Patterned Sensory Enhancement (PSE) technique on the motor function of the affected upper limb in hemiplegic stroke patients by comparing the use of PSE and simple rhythmic cue. A total of 16 stroke patients were recruited from rehabilitative hospitals. The participants were assigned to the experimental group (n = 8) and control group (n = 8). While performing six different upper limb motions, musical stimuli applying the PSE technique was presented for the experimental group and simple rhythmic cue using the metronome was applied for the control group. The results showed that while the significantly increased range of motion (ROM) was found in the experimental group with the immediate use of PSE (p < .05), the control group did not show no significant change. This study implies that the use of musical elements in cueing for upper limb motion immediately leads to significant improvement in ROM by providing sufficient temporal, spatial, and dynamic information for expected motor performance.

• Journal of The Korean Society of Integrative Medicine
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• v.8 no.4
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• pp.291-305
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• 2020

Purpose : The duration of speech segments reflects children's speech motor development. The purpose of this study was to determine whether segmental sound and word duration varies by age among preschool children. Methods : A total of 60 children aged 4~5 years participated in this study. Participants took the picture-naming test to produce single-word speech data. The duration of the consonant at the initial position of the word and the final position of the word, the voice onset time of plosive, the duration of the vowel following the initial consonant, and the duration of the word were measured. Results : As age increased, the duration of the initial consonant, the duration of the word, and the voice onset time decreased significantly. The main effects of age, manner of articulation, and place of articulation on the duration of the initial consonant were significant. The duration of consonants in the nasal sound and plosives and the duration of bilabial and alveolar sound differed significantly between groups. The main effects of age and vocal type on voice onset time were significant. The main effect of age on the duration of the consonant in the final position of word and on the duration of the vowel were not statistically significant. Conclusion : The results of this study showed that the duration of segmental sound and the word were associated with speech development between 4 and 5 years old. Accordingly, duration of the segmental sound and the word may serve as an acoustic cue as they reflect speech development and speech motor control maturity.

• The Journal of Korean Physical Therapy
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• v.34 no.3
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• pp.98-103
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• 2022

Purpose: New therapeutic approaches have emerged to improve gait ability in patients with brain damage, such as action observation learning (AOT), auditory cueing, motor imagery etc. We attempted to investigate the effects of AOT with auditory cueing (AOTAC) on gait function in patients with stroke. Methods: The eighteen stroke patients with a unilateral hemiparesis were randomly divided into three groups; the AOTAC, AOT, and control groups. The AOTAC group (n=8) received training via observing a video that showed normal gait with sound of footsteps as an auditory cue; the AOT group (n=6) receive action observation without auditory stimulation; the control group (n=5) observed the landscape video image. Intervention time of three groups was 30 minutes per day, five times a week, for four weeks. Gait parameters, such as cadence, velocity, stride length, stance phase, and swing phase were collected in all patients before and after each training session. Results: Significant differences were observed among the three groups with respect to the parameters, such as cadence, velocity, stride length, and stance/swing phase. Post-hoc analysis indicated that the AOTAC group had a greater significant change in all of parameters, compared with the AOT and control groups. Conclusion: Our findings suggest that AOTAC may be an effective therapeutic approach to improve gait symmetry and function in patients with stroke. We believe that this effect is attributable to the change of cortical excitability on motor related to cortical areas.

• Asia Marketing Journal
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• v.15 no.3
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• pp.159-172
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• 2013

In the present study, we apply the multiple cue probability learning (MCPL) paradigm to examine consumer learning from feedback in repeated trials. This paradigm is useful in investigating consumer learning, especially learning the relationships between the overall quality and attributes. With this paradigm, we can analyze what people learn from repeated trials by using the lens model, i.e., whether it is knowledge or consistency. In addition to introducing this paradigm, we aim to demonstrate that knowledge people gain from repeated trials with feedback is robust enough to weaken one of the most often examined contextual effects, the asymmetric dominance effect. The experiment consists of learning session and a choice task and stimuli are sport rafting boats with motor engines. During the learning session, the participants are shown an option with three attributes and are asked to evaluate its overall quality and type in a number between 0 and 100. Then an expert's evaluation, a number between 0 and 100, is provided as feedback. This trial is repeated fifteen times with different sets of attributes, which comprises one learning session. Depending on the conditions, the participants do one (low) or three (high) learning sessions or do not go through any learning session (no learning). After learning session, the participants then are provided with either a core or an extended choice set to make a choice to examine if learning from feedback would weaken the asymmetric dominance effect. The experiment uses a between-subjects experimental design (2 × 3; core set vs. extended set; no vs. low vs. high learning). The results show that the participants evaluate the overall qualities more accurately with learning. They learn the true trade-off rule between attributes (increase in knowledge) and become more consistent in their evaluations. Regarding the choice task, there is a significant decrease in the percentage of choosing the target option in the extended sets with learning, which clearly demonstrates that learning decreases the magnitude of the asymmetric dominance effect. However, these results are significant only when no learning condition is compared either to low or high learning condition. There is no significant result between low and high learning conditions, which may be due to fatigue or reflect the characteristics of learning curve. The present study introduces the MCPL paradigm in examining consumer learning and demonstrates that learning from feedback increases both knowledge and consistency and weakens the asymmetric dominance effect. The latter result may suggest that the previous demonstrations of the asymmetric dominance effect are somewhat exaggerated. In a single choice setting, people do not have enough information or experience about the stimuli, which may lead them to depend mostly on the contextual structure among options. In the future, more realistic stimuli and real experts' judgments can be used to increase the external validity of study results. In addition, consumers often learn through repeated choices in real consumer settings. Therefore, what consumers learn from feedback in repeated choices would be an interesting topic to investigate.

• Journal of Music and Human Behavior
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• v.16 no.1
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• pp.47-72
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• 2019

This study reviewed and analyzed English-written studies using a rhythmic tapping task for motor control of children with autism spectrum disorder (ASD). Inclusion criteria for the participants were children with ASD and typically developing (TD) children. The keywords used for the outcome variables included rhythmic tapping, timed movement, and synchronization. Ten studies were included in the final analysis. The included studies were analyzed in terms of target variables, auditory stimuli, and measurements. A meta-analysis was also conducted to examine how children with ASD performed rhythmic tapping tasks compared to children with TD. In the identified studies, five variables were used: timed movement control, timing reproduction, bimanual coordination, synchronization, and interpersonal synchronization. It was found that rhythmic tapping performance was analyzed in terms of accuracy and precision of the movement and reported as significantly correlated to social skills measures. The meta-analysis results showed that there were no significant differences between the ASD and TD groups in continuing rhythmic movements when the presented auditory stimuli ended, whereas there were significant group differences in their ability to maintain their motor performance consistently and to synchronize with auditory cue or with others. These results support the rhythmic tapping task as an effective measure for not only motor control but also social skills development in children with ASD.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.14 no.1
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• pp.344-350
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• 2013

A recent study reporting significantly reduced symmetry in arm swing amplitude in early Parkinson's disease (PD), as measured during gait by auditory cues velocity, led to this investigation of arm swing symmetry and amplitude in PD. The subjects were 14 elderly patients diagnosed with PD. Patients were measured of three conditions performed in random order: slow, general, fast. The auditory cue velocity consisted of a metronome beat ${\pm}20%$ than the subject's general gait speed. Using a motion analysis measurement system, changes in kinematic variables were compared to arm swing analysis. PD groups showed a highly significant reduction of the arm swing amplitude on the more affected body side(MAS)(p<.05). Comparison between the auditory cues velocity, there was a significant increase arm swing amplitude in fast velocity gait than slow and general velocity gait(p<.05). We conclude that motion analysis during gait by auditory cues velocity allows reliable investigation of asymmetric arm movements in early PD patients which attenuate with ongoing disease. The measurement of limb kinematics during gait by auditory cues velocity can broaden our methodological line-up for the analysis of complex motor programs in movement disorders.