• Food Science and Preservation
• /
• v.18 no.6
• /
• pp.1002-1008
• /
• 2011

This study aimed to examine the effect of the Schizandra chinensis fruit and Morus alba leaf on insulin expression in HIT-T15 cells, which is exposed by glucose. The total polyphenol contents of the S. chinensis fruit ethanol extract and the M. alba leaf hot-water extract were $20.11{\pm}0.35$ mg/g and $50.02{\pm}0.62$ mg/mL, respectively. The S. chinensis fruit ethanol extract and the M. alba leaf hot-water extract contained $2.85{\pm}0.15$ and $8.76{\pm}0.43$ mg/g flavonoids, respectively. The antioxidant ability of the M. alba leaf hot-water extract was found to be superior to that of the S. chinensis fruit ethanol extract. Compared to the HIT-T15-treated 10 mM 2-deoxy-D-glucose, the $100{\mu}g/mL$ S. chinensis ethanol extract was found to have a two fold increase in insulin productivity. Moreover, the $100{\mu}g/mL$ M. alba leaf hot-water extract promoted the insulin secretion of high-glucose-damaged HIT-T15 almost ten fold. The above results showed that the S. chinensis fruit ethanol extract and M. alba leaf hot-water extract may improve the insulin productivity of the beta cell with glucose-induced oxidative damage. These data suggest that the S. chinensis fruit ethanol extract and the M. alba leaf hot-water extract can be used as food materials for the regulation of insulin secretion.

• Journal of the Korean Applied Science and Technology
• /
• v.34 no.4
• /
• pp.1025-1035
• /
• 2017

In this study, the hot water extract from Mulberry (Morus alba) Leaves fermented with Hericium erinaceum mycelium (MA-HE) was assessed for antioxidant activity. Radical scavenging activity of MA-HE evaluated using 2,2-diphenyl-1-picrylhydrazyl(DPPH) radical and 2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid)(ABTS) radical. MA-HE showed 63% DPPH radical scavenging activity at $500{\mu}g/mL$ and 98.27% ABTS radical scavenging activity at $250{\mu}g/mL$. MA-HE was shown to significantly inhibited DNA strand breakage induced by free radical. MA-HE also inhibited free radical-mediated human serum albumin modification. MA-HE effectively inhibited $H_2O_2$ induced cell death and significantly increased of the 8% cell survival at $100{\mu}g/mL$. MA-HE decreased intracellular reactive oxygen species (ROS) levels in $H_2O_2$-treated cells. The results suggested that MA-HE can contribute to antioxidant and protected cells from oxidative stress-induced cell injury.

• International Journal of Industrial Entomology and Biomaterials
• /
• v.3 no.2
• /
• pp.135-139
• /
• 2001

Experiments were held in Egypt to test a new Package of practices for commercial cocoon production, including adding secondary macro and micronutrients to the mulberry garden, disease free rearing regime, low temperature rearing in young instars and natural mounting fur silkworms. This package for seed silkworm rearing lead to increase in fecundity by 67-121eggs (15.12-26.22%) and yield of standard boxes per 1 parent egg boxes by 57-58 egg boxes(48.33-51.66%) respectively in comparison th the traditional cocoon production technology.

• International Journal of Industrial Entomology and Biomaterials
• /
• v.22 no.2
• /
• pp.39-44
• /
• 2011

To evaluate the growth rate of larval and pupal parameters of silkworm Bombyx mori fed with Silver Nanoparticles (AgNps) treated $MR_2$ mulberry leaves, the following works have been considered. The AgNp was synthesized by chemical reduction method, it was diluted by different concentrations such as 25%, 50%, 75% and 100% (without dilution). Fresh mulberry leaves (Morus alba L.) were sprayed by each concentration and were fed to silkworms, from $3^{rd}$, $4^{th}$ and $5^{th}$ instar, five feedings/day. Group $T_1$ larvae received $MR_2$ mulberry leaves sprayed with distilled water and served as control, group $T_2$, $T_3$, $T_4$ and $T_5$ larvae received 25%, 50%, 75% and 100% AgNps sprayed mulberry leaves, respectively. Silkworm larvae fed on M. alba ($MR_2$) leaves sprayed with 25% concentration of AgNps (group $T_2$) was significantly increased the larvae and cocoon length, width and weight as compared to those fed on control (group $T_1$) $MR_2$ mulberry leaves and other groups ($T_3$, $T_4$ and $T_5$). Hence, 25% AgNps dose was fixed as an effective dose. It has been observed from the present study that 25% AgNps treated (group $T_2$) leaves fed by silkworms have enhanced the larval and pupal growth and quantity of silk production than control.

• Proceedings of the PSK Conference
• /
• 2002.10a
• /
• pp.377.2-377.2
• /
• 2002

The MeOH extract of the twig of Marus alba L. (Moraceae) inhibited strong lipid peroxidation activity. Five antioxidative compounds were isolated through activity-guided fractionation. and identified as 6-geranylapigenin (1). 6-geranylnorartocarpetin(2). resveratrol (3). oxyresveratrol (4). quercetin (5) by physicochemical and spectrometric methods. In order to evaluate the antioxidant effect of these compounds. the lipid peroxidation inhibitory activity test were performed. Compounds 1-5 showed greater activity than tocopherol. (omitted)

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.44 no.6
• /
• pp.823-831
• /
• 2015

Evaluation of biological activity and analysis of functional constituents from water and ethanol extracts of four different parts of mulberry (Morus alba L.) tree were carried out to develop functional ingredients and foods using extracts of mulberry tree. The water and ethanol extracts of four different parts of mulberry tree were prepared and their biological activities and functional constituents determined by in vitro assays and HPLC, respectively. In general, ethanol extracts showed stronger biological activities and higher functional constituents than water extracts. Ethanol extracts of mulberry fruit, root bark, and twig showed stronger antioxidant ($IC_{50}=128.4{\mu}g/mL$), ${\alpha}$-glucosidase ($IC_{50}=12.0{\mu}g/mL$), and lipoxygenase ($IC_{50}=36.3{\mu}g/mL$) and tyrosinase ($IC_{50}=410.3{\mu}g/mL$) inhibitory activities, respectively, than those of other parts. Mulberry fruit and leaf showed the highest contents of anthocyanin (cyanidin 3-glucoside: 213.20 mg/100 g) and chlorogenic acid (514.97 mg/100 g), and especially ethanol extract of mulberry leaf contained higher quercetin 3-O-(6-O-malonyl)glucoside (143.25 mg/100 g) and kaempferol 3-O-(6-O-malonyl)glucoside (30.25 mg/100 g) contents without water extract of mulberry leaf. Meanwhile, mulberry twig contained both oxyresveratrol glycoside (48.90 mg/100 g) and its aglycone (21.88 mg/100 g), whereas mulberry root bark contained mostly oxyresveratrol glycoside (724.05 mg/100 g). Additionally, mulberry root bark and leaf contained much higher ${\gamma}$-aminobutyric acid (223.90 mg/100 g) and 1-deoxynojirimycin (86.07 mg/100 g) contents, respectively, than other parts of mulberry tree. These results suggest that high quality processed foods and functional foods using mixtures of mulberry fruits, leaves, twigs, and root barks should be developed for prevention and inhibition of several pathological disorders.

• Journal of Sericultural and Entomological Science
• /
• v.31 no.1
• /
• pp.1-11
• /
• 1989

To investigate changes of dry weight, C/N ratio, carbohydrate, ratein and nucleic acids in the barks of the hardwood cutting of mulberry plants, the barks of the hardwood cutting were taken every 10days from the cutting day to 40days after cutting. Results were summarized as follows : Growth of shoot and root in Keomseolppong (Morus bimbycis KoIDZ.) after cutting was better than that in kaeryangppon (Morus alba L.) and Daeryugppong(Morus Lhou(SER.) KoIDZ.). Dry weight in the barks of the hardwood cutting decreased up to 40days after cutting (decrease percentage of dry weight : about 14%) and Keomiseolppong showed the highest decrease precentage of dry weight. Change of C/N ratio decreased in the initiation, whereas that from 10days after cutting increased with the growth of new shoot, C/N ratio was the highest in Keomseolppong and the lowest in Kaeryangppong. Total sugar showed decreasing tendency up to 40 days after cutting the increase from 10 days to 20 days. Reducing sugar increased in the frist 20days, followed gradually decrease up to 40 days after cutting Starch levels decreased up to 40 days. Change in the content of starch closely paralleled to that of dry weight of bark of the hardwood cutting. Content of total sugar and starch in Keomseolppong was the highest, and was consumed more than that in the rest of varieties. Crude protein and soluble protein increased up to 10 days after cutting, followed decrease up to 40 days. Protein content of Keomseolppong was smaller than that in the rest of varieties. RNA content showed the same tendency with that of protein content, but Keomseolppong showed always the highest. Content of DNA did not show any change and difference among three varieties.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
• /
• v.15 no.2
• /
• pp.80-103
• /
• 2002

To screen the effective materials for hair loss treatment, several natural extracts were tested using in vivo and in vitro test models. Firstly, all test materials were applicated onto the back skin of C57BL／6 mouse and then hair growth pormoting effect were measured using hair growth index As a result, Polygonum muitifiorum Thunb and Terrninalia chebula Retz. showed potent hair growth promoting effect, ranking as 1.5-2.0 of hair growth index. However, there were no plant extracts, which have remarkable potential of growth promotion of human hair dermal papilla cells cultured in vitro. In the experiments of 5${\alpha}$-reductase type Ⅱ inhibition assay, Morus alba L., Chaenomelis Fructus, Saussureae Radix, Angelicae Gigantis Radix, Polygonum multifiorum Thunb, and Angelica dahurica (Fischer) Bentham et Hooker f. showed effective potential to inhibit the activity of 5${\alpha}$-reductase type Ⅱ. To investigate the possible involvement of effects of several plant extracts on the gene expression of growth factors in human hair dermal papilla cells, RT-PCR analyses were performed. As a consequences, Mentha haplocalyx Briq., Cimicifuga foetida L., Eclipta prostrata (L.) L., Pinus densiflora S. et. Z, and Polygonum muitifiorum Thunb revealed the regulatory roles on the expression of growth factors such as IGF-I, KGF, HGF and VEGF in the dermal papilla cells. Another test for inhibition of microbial such as P. acne and P. ovale were also carried out to find whether these plant extracts have anti-microbial activities. Morus alba L. and Chaenomelis Fructus showed anti-microbial effects on Propionibacterium acnes, which is believed as a pathogen of acne. Together, these results showed several plant extracts can be used for hair growth promotion.

• Preventive Nutrition and Food Science
• /
• v.17 no.4
• /
• pp.280-285
• /
• 2012

Six polyphenolic compounds, such as chlorogenic acid (CA), rutin (RT), isoquercitrin (IQT), quercetin-3-O-(6-O-malonyl)-${\beta}$-D-glucoside (QMG), astragalin (AG), kaempferol-3-O-(6-O-malonyl)-${\beta}$-D-glucoside (KMG), were isolated from mulberry leaves by a series of isolation procedures, such as Diaion HP-20, silica-gel, Sephadex LH-20, and ODS-A column chromatographies. The chemical structures of the phenolic compounds were identified by UV and NMR spectral analyses. Levels of polyphenols in mulberry leaves from six different mulberry cultivars ranged from 1,042.16 to 1,871.97 mg% per dry weight; Guksang cultivar showed the highest levels of polyphenols, whereas Gaeryangdaehwa contained the least polyphenol contents. Generally, levels of polyphenols in mulberry leaves decreased with increasing harvest time, except for Yoolmok, but increased with heat processing time, except QMG and KMG. These results suggest that the heat processed mulberry leaves of Guksang cultivar harvested in early May can be potentially useful sources for production of high quality mulberry leaf teas.

• Journal of the East Asian Society of Dietary Life
• /
• v.25 no.4
• /
• pp.643-650
• /
• 2015

The principal objective of this study was to evaluate the quality of Yukwa prepared with mulberry leaf (Morus alba Linne) powder. The moisture, crude protein, crude lipid, crude ash, dietary fiber, and rutin contents of used mulberry leaf powder were 6.65%, 19.50%, 3.26%, 18.24, 38.91 and 0.54%, respectively. The tested concentrations of mulberry leaf powder were 0, 3, 5, and 7%. The Yukwa were stored at $45^{\circ}C$ for 40 days. Yukwa dough prepared with mulberry leaf powder had a lower pH compared to the control group. As more mulberry leaf powder was added, the spread ratio, moisture content, and volume increased. As more mulberry leaf powder was added, L-value decreased while a and b values increased with increasing mulberry leaf powder content. The hardness of Yukwa made with 7% mulberry leaf powder was the highest among the samples. In the sensory tests, Yukwa with 5% mulberry leaf powder received the highest scores. The acid value and peroxide values were lower in Yukwa prepared with mulberry leaf powder than control. Yukwa prepared with mulberry leaf powder strongly inhibited oxidation of lipids. The thiobarbituric values were lower in Yukwa prepared with 5% mulberry leaf powder than in Yukwa prepared with 3% and 7% mulberry leaf powders and control Yukwa. These results suggest that mulberry leaf powder can be applied to Yukwa to achieve high quality and functionality.