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버섯균사체 발효 뽕잎 추출물의 항산화 활성

Antioxidant Activity of Mulberry Leaves Extract Fermented by Hericium erinaceum

  • 강정훈 (청주대학교 바이오메디컬학과)
  • 투고 : 2017.11.18
  • 심사 : 2017.12.12
  • 발행 : 2017.12.30

초록

본 연구에서는 뽕잎(Morus alba)을 노루궁뎅이 버섯균사체(Hericium erinaceum)로 발효시킨 열수 추출물(MA-HE)의 항산화 활성을 확인하고자 하였다. MA-HE의 항산화 활성을 DPPH radical, ABTS radical 소거활성 측정을 통해 알아보았다. 그 결과 DPPH radical 소거활성은 $500{\mu}g/mL$ 농도에서 61.73%, ABTS radical 소거활성은 $250{\mu}g/mL$ 농도에서 97.39%로 나타났다. MA-HE은 DNA의 산화적 손상을 효과적으로 억제하였다. 또한 생체고분자물질인 사람의 혈청단백질의 산화적 손상을 억제하였다. 세포에 $H_2O_2$를 처리하였을 때 세포생존율에 비하여 발효물을 $100{\mu}g/mL$ 농도로 전 처리한 세포생존율은 8% 증가했으며, 발효물을 $50{\mu}g/mL$ 농도로 처리했을 경우 세포 내 활성산소의 축적이 유의적으로 감소되었다. 본 연구 결과는 MA-HE이 항산화 활성을 가지고 있으며 산화적 스트레스에 의해 야기되는 세포 독성에 대한 보호 작용도 뛰어난 것으로 사료되었다.

In this study, the hot water extract from Mulberry (Morus alba) Leaves fermented with Hericium erinaceum mycelium (MA-HE) was assessed for antioxidant activity. Radical scavenging activity of MA-HE evaluated using 2,2-diphenyl-1-picrylhydrazyl(DPPH) radical and 2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid)(ABTS) radical. MA-HE showed 63% DPPH radical scavenging activity at $500{\mu}g/mL$ and 98.27% ABTS radical scavenging activity at $250{\mu}g/mL$. MA-HE was shown to significantly inhibited DNA strand breakage induced by free radical. MA-HE also inhibited free radical-mediated human serum albumin modification. MA-HE effectively inhibited $H_2O_2$ induced cell death and significantly increased of the 8% cell survival at $100{\mu}g/mL$. MA-HE decreased intracellular reactive oxygen species (ROS) levels in $H_2O_2$-treated cells. The results suggested that MA-HE can contribute to antioxidant and protected cells from oxidative stress-induced cell injury.

키워드

참고문헌

  1. C. H. Jeong, O. S. Joo, K. H. Shim, "Chemical components and physiological activities of young mulberry stem", J. Korean Food Preserv., Vol.9, No.2 pp. 228-233, (2002).
  2. H. B. Kim, C. K. Kang, G. B. Sung, S. W. Kang, J. R. Lee, "Antioxidative capacity of mulberry leaf and its tea", J. Seri. Ento. Sci., Vol.49, No.1 pp. 18-22, (2007).
  3. D. C. Kim, M. J. In, H. J. Chae, "Preparation of mulberry leaves tea and its quality characteristics", J. Appl. Biol. Chem., Vol.53, No.1 pp. 56-59, (2010). https://doi.org/10.3839/jabc.2010.010
  4. J. Y. Chae, J. Y., Lee, I. S. Hoang, D. Whangbo, P. W. Choi, W. C. Lee, J. W. Kim, S. Y. Kim, S. W. Choi, S. J. Rhee, "Analysis of functional components of leaves of different mulberry cultivars", J. Korean Soc. Food Sci. Nutr., Vol.32, No.1 pp. 15-21, (2003). https://doi.org/10.3746/jkfn.2003.32.1.015
  5. M. Y. Park, K. S. Lee, M. K Sung, "Effects of dietary mulberry, Korean red ginseng, and banaba on glucose homeostatis in relation to PPAR-${\alpha}$, PPAR-${\gamma}$, and LPL mRNA expressions", Life Sci., Vol.77, No.26 pp. 3344-3354, (2005). https://doi.org/10.1016/j.lfs.2005.05.043
  6. J. R. Lee, Y. J. Hah, J. W. Lee, Y. M. Song, S. K. Jin, I. S. Kim, K. H. Hah, S. J. Kwak, "Physico-chemical and sensory properties of emulsified sausages containing mulberry and persimmon leaf powder", Korean J. Food Sci. Ani. Resour., Vol.22, No.4 pp. 330-336, (2002).
  7. C. Hansawasdi, J. Kawabata, "${\alpha}$-Glucosidase inhibitory effect of mulberry (Morus alba) leaves on Caco-2", Fitoterapia, Vol.77, No.7-8 pp. 568-573, (2006). https://doi.org/10.1016/j.fitote.2006.09.003
  8. M. G. Kang, W. S. Jo, W. H. Kim, S. Y. Choi, S. D. Park, "The difference of occurring pattern of Hericium erinaceus by pinheading induction methods", J. Mushrooms, Vol.13, No.1 pp. 11-15, (2015). https://doi.org/10.14480/JM.2015.13.1.11
  9. T. Mizuno, T. Wasa, H Ito, C. Suzuki, N. Ukai, "Antitumor-active polysaccharides isolated from the fruiting body of Hericium erinaceum, an edible and medicinal mushrooms called yamabushiotake or houtou", Biosci. Biotechnol. Biochem., Vol.56, No.2 pp. 347-348, (1992). https://doi.org/10.1271/bbb.56.347
  10. T. Mizuno,"Yamabushitake, Hericium rinaceum: Bioactive substances and medicinal utilization", Food Reviews International, Vol.11 pp. 173-178, (1995). https://doi.org/10.1080/87559129509541027
  11. K. Mori, Y. Obara, M. Hirota, Y. Azumi, S. Kinugasa, S. Inatomi, N. Nakahata, "Nerve growth factor-inducing activity of Hericium erinaceus in 1321N1 human astrocytoma cells", Biol. Pharm. Bull., Vol.31, No.9 pp. 1727-1732, (2008). https://doi.org/10.1248/bpb.31.1727
  12. K. Mori, S. Inatomi, K. Ouchi, Y. Azumi, T. Tuchida, "Improving effects of the mushroom Yamabushiotake (Hericium erinaceus) on mild cognitive impairment: a double-blind placebo-controlled clinicaltrial", Phytoher. Res., Vol.23, No.3 pp. 367-372, (2009). https://doi.org/10.1002/ptr.2634
  13. H. Kawagishi, A. Shimada, S. Hosokawa, H. Mori, H. Sakamoto, Y. Ishiguro, S. Sakemi, J. Bordner, N. Kojima, S. Furukawa, Erinacines E, F and G, "Stimulators of nerve growth factor (NGF)-synthesis, from the mycelia of Hericium erinaceum", Tetrahedron Letters, Vol.37, No.41 pp. 7399-7402, (1996). https://doi.org/10.1016/0040-4039(96)01687-5
  14. J. H. Ha, H. S. Jeog, S. H. Oh, S. S. Jeong, M. H. Jeong, H. S. Jeong, J. H. Jeong, K. W. Yu, H. Y. Lee, "Comparision of Immuno activities of fresh Ginseng cultured Phelinus linteus and Hericium erinaceum mycelium associated whit ultrasonification extraction", Korean J. Medicinal Crop. Sci., Vol.17, No.5 pp. 311-320, (2009).
  15. C. K. Park, H. Kim, Qi. Tu, K. W. Yu, H. S, Jeong, H. Y. Lee, J. H Jeong, "Chemical composition and immunostimulating activity of the fermented Korean Ginseng (Panax ginseng C.A. Meyer) with mushroom mycelium by solid culture", J. Korean Soc. Food Sci. Nutr., Vol.38, No.9 pp. 1145-1152, (2009). https://doi.org/10.3746/jkfn.2009.38.9.1145
  16. Y. Jang, S. H. Shin, B. I. Choi, D. S. Park, J. H. Jeon, Y. M. Cho, J. H. Cho, D. H. Jung, S. Y. Hwang, B. W. Ahn, M. S. Jeong, J. H. Jeong, Y. B. Kim, "Effects of Erinacol$^{(R)}$, Extract of Hericium erinaceum Cultivated with Artemisia iwayomogi, on Alcoholic Hangover and Immune Function", Lab. Anim. Res., Vol.22, No.2 pp. 157-163, (2006).
  17. S. H. Shin, J. Y. Jang, B. I. Choi, JH. H. Jeon, H. J. Ji, S. H. Moon, D. H. Jung, Y. G. Yeo, S. J. Baek, S. J. Hwang, J. H. Chung, Y. B. Kim, "Effects of Erinacol$^{(R)}$, Extract of Hericium erinaceum Cultivated with Artemisia iwayomogi, on Alcoholic Fatty Liver", Lab. Anim. Res., Vol.22, No.2 pp. 165-170, (2006).
  18. B. I. Choi, S. H. Shin, J. Y. Jang, D. S. Park, D. H. Kang, S. S Nahm, Y. G. Yeo, J. H. Cho, S. Y. Hwang, M. S. Jeong, M. S. Jeong, J. H. Jeong, Y. B. Kim, "Single-and repeated-dose toxicities of Erinacol$^{(R)}$, extract of Hericium erinacium cultivated with Artemisia iwayomogi, in rats", Lab. Anim. Res., Vol.22, No.2 pp. 171-179, (2006).
  19. S. C. Kwon, "Biological activities of ethanol extract from Hericium erinaceus mycelium on Angelica keiskei and Angelica keiskei pomace", J. Korean Soc. Food Sci. Nutr., Vol.40, No.12 pp. 1648-1653, (2011). https://doi.org/10.3746/jkfn.2011.40.12.1648
  20. S. S. Kim, I. G. Kyeong, M. L. Lee, D. G. Kim, J. Y. Shin, J. Y. Yang, G. H. Lee, W. S. Eum, J. H. Kang, "Protective effects of Sea Buckthorn (Hippophae rhamnoides L.) leaves fermented with Hericium erinaceum mycelium against oxidative modification of biological macromolecules and cell death", J. Korean Soc. Food Sci. Nutr., Vol.44, No.1 pp. 35-43, (2015). https://doi.org/10.3746/jkfn.2015.44.1.035
  21. W. Brand-Williams, M. E. Cuvelier, C. Berset, "Use of a free radical method to evaluate antioxidant activity", Food Sci. Technol., Vol.28, No.1 pp. 25-30, (1995).
  22. K. Hiramoto, H. Johkoh, K. Sako, K. Kikugawa, "DNA breaking activity of the carbon-centered radical generated from 2 , 2' - azobis(2-amidinopropane) hydrochloride (AAPH)", Free Radic. Res. Commun., Vol.19, No.5 pp. 323-332, (1993). https://doi.org/10.3109/10715769309056521
  23. E. H. Ahn, D. W. Kim, M. J. Shin, Y. N. Kim, H. R. Kim, S. J. Woo, S. M. Kim, J. Kim, J. Park, W. S. Eum, S. Y. Choi, "PEP-1-ribosomal protein S3 protects dopaminergic neurons in an MPTP-induced Parkinson's disease mouse model", Free Radic. Biol. Med., Vol.55 pp. 36-45, (2013). https://doi.org/10.1016/j.freeradbiomed.2012.11.008
  24. M. J. Kim, D. W. Kim, J. H. Park, S. J. Kim, C. H. Lee, J.I. Yong, E. J. Ryu, S. B. Cho, H. J. Yeo, J. Hyeon, S. W. Cho, D. S. Kim, O. Son, J. Park, K. H. Han, Y. S. Cho, W. S. Eum, S. Y. Choi, "PEP-1-SIRT2 inhibits inflammatory response and oxidative stress-induced cell death via expression of antioxidant enzymes in murine macrophages", Free Radic. Biol. Med., Vol.63 pp. 432-445, (2013). https://doi.org/10.1016/j.freeradbiomed.2013.06.005
  25. H. Aoshima, H. Tsunoue, H, HKoda, Y. Kiso, "Aging of whiskey increases 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity", J. Agric. Food Chem., Vol.52, No.16 pp. 5240-5244, (2004). https://doi.org/10.1021/jf049817s
  26. H. K. Kim, Y. E. Kim, J. R. Do, Y. C. Lee, B. Y. Lee, "Antioxidative activity and physiological activity of some Korean nedicinal plants", Korean J. Food Sci. Technol., Vol.27, No.1 pp. 80-85, (1995).
  27. Y. J. Oh, H. R. Seo, Y. M. Choi, D. S. Jung, "Evaluation of antioxidant activity of the extracts from the aerial parts of Cnidium officinale Makino', Korean J. of Medicinal Crop. Science, Vol.18, No.6 pp. 373-378, (2010).
  28. B. J. An. J. T. Lee, S. A. Lee, J. H. Kwak, J. M. Park, J. Y. Lee, J. H Son, "Antioxidant effects and application as natural ingredients of Korean Sanguisorbae officinalis L.", J. Kor. Soc. Apple. Biol. Chem., Vol.47, No.2 pp. 244-250, (2004).
  29. O. Hassan, L. S. Fan, "The anti-oxidation potential of polyphenol extract from cocoa leaves on mechanically deboned chicken meat (MDCM)", LWT-Food Sci. Technol., Vol.38, No.4 pp. 315-321, (2005). https://doi.org/10.1016/j.lwt.2004.06.013
  30. T. A. Dix, J. Aikens, "Mechanisms and biological relevance of lipid peroxidation initaiation", Chem. Res. Toxicol., Vol.6, No.1 pp. 2-18, (1993). https://doi.org/10.1021/tx00031a001
  31. B. N. Ames, "Dietary carcinogens and anticarcinogens. Oxygen radicals and degenerative diseases", Science, Vol.221, No.4617 pp. 1256-1264, (1983). https://doi.org/10.1126/science.6351251
  32. P. A. Cerutti, "Prooxidant states and tumor promotion", Science, Vol.227, No.4685 pp. 375-381, (1985). https://doi.org/10.1126/science.2981433
  33. J. L. Sagripanti, K. H. Kraemer, "Site-specific oxidative DNA damage at polyguanosines produced by copper plus hydrogen peroxide", J. Biol. Chem., Vol.264, No.3 pp. 1729-1734, (1989).
  34. T. Finkel, N. J. Holbrook, "Oxidants, oxidative stress and the biology of ageing", Nature, Vol.408, No.6809 pp. 239-247, (2000). https://doi.org/10.1038/35041687
  35. S. Kawanishi, Y. Hiraku, S. Oikawa, "Mechanism of guanine-specific DNA damage by oxidative stress and its role in carcinogenesis and aging", Mutat. Res., Vol.488, No.1 pp. 65-76, (2001). https://doi.org/10.1016/S1383-5742(00)00059-4
  36. E. R. Stadtman, B. S. Berlett, "Fenton chemistry. Amino acid oxidation", J. Biol. Chem., Vol.266, No.26 pp. 17201-17211, (1991).
  37. K. J. Davies, M. E. Delsignore, "Protein damage and degradation by oxygen radicals. III. Modification of secondary and tertiary structure", J. Biol. Chem., Vol.262, No.20 pp. 9908-9913, (1987).
  38. R. L. Levine, C. N. Oliver, R. M. Fulks, E. R. Stadtman, "Turnover of bacterial glutamine synthetase: oxidative inactivation precedes proteolysis", Proc. Natl. Acad.Sci. U S A, Vol.78, No.4 pp. 2120-2124, (1981). https://doi.org/10.1073/pnas.78.4.2120
  39. A. J. Rivett, R. L. Levine, "Metal-catalyzed oxidation of Escherichia coli glutamine synthetase: correlation of structural and functional changes", Arch. Biochem. Biophys., Vol.278, No.1 pp. 26-34, (1990). https://doi.org/10.1016/0003-9861(90)90226-O
  40. M. E. Sitar, S. Aydin, U. Cakatay, "Human serum albumin and its relation with oxidative stress", Clin. Lab., Vol.59, No.9-10 pp. 945-952, (2013)
  41. R. E. Racifici, K. J. Davies, "Protein degradation as an index of oxidative stress", Methods Enzymol, Vol.186 pp. 485-502, (1990)
  42. H. Terawaki, K. Yoshimura, T. Haseqawa, Y. Matsuyama, T. Neqawa, K. Yamada, M. Matsushima, M. Nakayam, T. Hosoya, S. Era, "Oxidative stress is enhanced in correlation with renal dysfunction: examination with the redox state of albumin", Kidney Int., Vol.66, No.5 pp. 1988-1993, (2004). https://doi.org/10.1111/j.1523-1755.2004.00969.x
  43. D. R. Rosen, T. Siddique, D. Patterson, D. Figlewiez, P. Sapp, A. Hentati, D. Donaldson, J. Goto, J. P. O'Regan, H. X. Deng, Z. Rahmani, A. Krizus, D. McKJenna-Yasek, A. Cayabyab, S. M. Gaston, R. Berger, R. E. Tnzi, J. J. Halperin, B. Herzfeldt, R. Van den Bergh, W. Y. Hung, T. Bird, G. Deng, D. W. Mulder, C. Smyth, N. G. Laing, E. Soriano, M. A. Pericak-Vance, J. Hains, G. A. Rouleau, J. S. Gusella, H. R. Horvitz, and R. H. Jr. Brown, "Mutation in Cu,Zn-superoxide dismutase gene are associated wigh familial amyotrophic lateral sclerosis", Nature, Vol.362, No. pp. 59-62, (1993). https://doi.org/10.1038/362059a0
  44. B. Halliwell, J. M. Gutteridge, "The importance of free radicals and catalytic metal irons in human disease", Mol. Aspects Med., Vol.8, No.2 pp. 89-193, (1985). https://doi.org/10.1016/0098-2997(85)90001-9
  45. Z. Radak, S. Kumagai, A. W. Taylor, H. Naito, S. Goto, "Effects of exercise on brain function: role of free radical", Appl. Physiol. Nutr. Metab., Vol.32, No.5 pp. 942-946, (2007). https://doi.org/10.1139/H07-081
  46. C. F. Chen, S. Y. Lang, P. P. Zuo, N. Yang, X. Q. Wang, C. Xia, "Effects of D-galactose on the expression of hippocampal peripheral-type benzodiazepine receptor and spatial memory performances in rats", Psychoneuroendocrinology, Vol.31, No.7 pp. 805-811, (2006). https://doi.org/10.1016/j.psyneuen.2006.03.004
  47. X. Cui, P. Zuo, Q. Zhang, X. Li, Y. Hu, J. Long, L. Packer, J. Liu, "Chronic systemic D-galactose exposure induces memory loss, neurodegeneration, and oxidative damage in mice: protective effects of R-alpha-lipoic acid", J. Neurosci. Res., Vol.83, No.8 pp. 1584-1590, (2006). https://doi.org/10.1002/jnr.20845
  48. W. S. Sun, H. Q. Yu, H. Zhang, Y. L. Zheng, J. J. Wang, L. Luo, "Quercetin attenuates spontaneous behavior and spatial memory impairment in D-galactose-treated mice by increasing brain antioxidant capacity", Nutrition Research, Vol.27, No.3 pp. 169-175, (2007). https://doi.org/10.1016/j.nutres.2007.01.010